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The map label for this gene is tam [H]

Identifier: 187930684

GI number: 187930684

Start: 3789850

End: 3790455

Strand: Reverse

Name: tam [H]

Synonym: Rpic_3620

Alternate gene names: 187930684

Gene position: 3790455-3789850 (Counterclockwise)

Preceding gene: 187930686

Following gene: 187930679

Centisome position: 96.14

GC content: 67.49

Gene sequence:

>606_bases
ATGCAGCATCACGCCGCTCGCCTTGAGCGCGCCATCGAGCAGGCTGCGGTTGCCGCTGACCTCGCCGGCAAAGATGAGCG
GGTGGGGCTGCAAGGCTTGTCACCGGATGCGGCGCCGCTTGGCCAGCGGATGATCGTCGCGGCAGACGAGGACATACGGA
TCGTTCGTCAGCGGCCGAAGCTGATCGGCAAGCTGGCGGCGGGTGGCCACCTGGCTGTGCAGATGCCGGACAACCTTGAC
GAACCGGCGCACCGCCTGATGCGCGAAGTGGCCGCTGTGGGGCCGTGGGCCGGCAAGCTCAAGGGGGTGGAACGGACGGA
GCGCTTCGATGCGCGCCGCTATTACGCGCTGCTCTCGCCGCTGTGCTCGCGCGTCGACGTGTGGCGCACCACGTACATGC
ATCCGCTGCAAGGCGGCGCCGATGCGGTGGTCGAATGGTTCAAGGGCAGCGCGCTGCGGCCATTCCTCGCAGCGCTGAGC
GAGGACGAGCGCCCCGTATTCCTCACGCGCTATCGCGACATGATTGCCCAGGCCTATCCGGCACTCGACGATGGCACGGT
GCTGCTGCCGTTTCCGCGCCTGTTCATCGTGGCGACACGCAAGTAG

Upstream 100 bases:

>100_bases
ACGCGCTCTACTGAAGCGCGAGCCGCAGCACCACCGCCGCGCCCAGCCCTTCCGCCACCAACTCGAGCGCCGTTGAGCTG
CGCTGCACTTCGTAGAACGA

Downstream 100 bases:

>100_bases
GCGCAATCGCAGAGGCAGAAACGCCGGTGTCAAGTCCTGCCATAGGTTGACACTGTGCTCAGTCTCCGAGCGCCCGATGC
ACCGCATCGGGCGTTTTGTA

Product: Trans-aconitate 2-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MQHHAARLERAIEQAAVAADLAGKDERVGLQGLSPDAAPLGQRMIVAADEDIRIVRQRPKLIGKLAAGGHLAVQMPDNLD
EPAHRLMREVAAVGPWAGKLKGVERTERFDARRYYALLSPLCSRVDVWRTTYMHPLQGGADAVVEWFKGSALRPFLAALS
EDERPVFLTRYRDMIAQAYPALDDGTVLLPFPRLFIVATRK

Sequences:

>Translated_201_residues
MQHHAARLERAIEQAAVAADLAGKDERVGLQGLSPDAAPLGQRMIVAADEDIRIVRQRPKLIGKLAAGGHLAVQMPDNLD
EPAHRLMREVAAVGPWAGKLKGVERTERFDARRYYALLSPLCSRVDVWRTTYMHPLQGGADAVVEWFKGSALRPFLAALS
EDERPVFLTRYRDMIAQAYPALDDGTVLLPFPRLFIVATRK
>Mature_201_residues
MQHHAARLERAIEQAAVAADLAGKDERVGLQGLSPDAAPLGQRMIVAADEDIRIVRQRPKLIGKLAAGGHLAVQMPDNLD
EPAHRLMREVAAVGPWAGKLKGVERTERFDARRYYALLSPLCSRVDVWRTTYMHPLQGGADAVVEWFKGSALRPFLAALS
EDERPVFLTRYRDMIAQAYPALDDGTVLLPFPRLFIVATRK

Specific function: Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate [H]

COG id: COG4106

COG function: function code R; Trans-aconitate methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Tam family [H]

Homologues:

Organism=Escherichia coli, GI1787798, Length=142, Percent_Identity=44.3661971830986, Blast_Score=117, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR023149 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.144 [H]

Molecular weight: Translated: 22317; Mature: 22317

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHHAARLERAIEQAAVAADLAGKDERVGLQGLSPDAAPLGQRMIVAADEDIRIVRQRPK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
LIGKLAAGGHLAVQMPDNLDEPAHRLMREVAAVGPWAGKLKGVERTERFDARRYYALLSP
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHH
LCSRVDVWRTTYMHPLQGGADAVVEWFKGSALRPFLAALSEDERPVFLTRYRDMIAQAYP
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
ALDDGTVLLPFPRLFIVATRK
CCCCCEEEEECCCEEEEEECC
>Mature Secondary Structure
MQHHAARLERAIEQAAVAADLAGKDERVGLQGLSPDAAPLGQRMIVAADEDIRIVRQRPK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
LIGKLAAGGHLAVQMPDNLDEPAHRLMREVAAVGPWAGKLKGVERTERFDARRYYALLSP
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHH
LCSRVDVWRTTYMHPLQGGADAVVEWFKGSALRPFLAALSEDERPVFLTRYRDMIAQAYP
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
ALDDGTVLLPFPRLFIVATRK
CCCCCEEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]