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The map label for this gene is priA [H]

Identifier: 187930562

GI number: 187930562

Start: 3665534

End: 3667831

Strand: Reverse

Name: priA [H]

Synonym: Rpic_3497

Alternate gene names: 187930562

Gene position: 3667831-3665534 (Counterclockwise)

Preceding gene: 187930563

Following gene: 187930561

Centisome position: 93.03

GC content: 67.45

Gene sequence:

>2298_bases
ATGACCACGTCCCAAGTCGACGCCGCGTTTTCTGATGCCAAGGTGTCGGCCTCCATTGCTCGCGTGGTGATTGATACGCC
GCTCGATGCGGTGTTCGATTACCGGTGTGGGCAGCCCGTGCTGCCGGGGCAATTGGTTGTGGTGCCGTTTGGCAACCGGC
GTGTGGCGGGGTTAGTCGTCGAAACCGCCGCGACCACCGATGTGCCGCAAGAAAAGCTGCGCGATGTCGAGCGGGTGCTC
GACTGGGTGCCGCCGCTCGACGCCGAATGGCGGGCCCTGGCCGAATTTGCTGCCGGCTACTACCACCGCGCGCTCGGCGA
AGTGACGTTACCGGCGCTCCCGCCGCTGTGGCGAACCCCGGGCAGTTGGGACAGTCTCGCCCGGCAGGCCACCGAAACCG
TCTACGTGATGTTTGAACCGGCCCGGGCAGCGTTGCTGGACAGCATTCCGAAACGTGCGTCTGGCGCCATGCGATTGGCC
GAAGCGCTGACCGGCGATGGCGAGCTGTCCACCAGCGCTGCTGTTGAACTGCACGGGCAAGCCGTCGCCAAGCTGCGGGA
TTGGACGTCCGTCGGCTGGCTGCGCGCAGAACTGCGGCCGAAGGCGCTGTTGCCGCAACCGCTCGAACTGCCACTGCCTT
CCATCGCGCCCGCACTGAACGACGAACAGACCGCCGCCGTGGCAGCCATTGCCCAGGCGGGGGCGGCCGGCAACTTCAGC
CCGTTCCTGTTGTACGGCGTCACCGGCAGTGGCAAGACCGAGGTTTACCTGCACGCCATCGCCGACGCCTTGGCGCGCGA
GCCCGCCGCACAGGTCCTGATGCTGGTGCCCGAGATCAACCTGACGCCACAACTGGAGGCGCGCATTGCGGCGCGTTTTC
CCAGTGTGCCCATGGCGGCGCTGCACAGTGGTCTGGCCGAACAGCCGCGTGCGCTGAACTGGCTGGCCGCCCATCGCGGT
GAGGCGCGCATCGTGCTTGGCACGCGGCTGGCGATGCTGGCGTCGCTGCCCAAGCTCGCGCTCATCCTCGTCGACGAGGA
ACACGACACTTCGTACAAGCAGCAGGAGGGCCTGCGCTATTCCGCACGCGACCTGGCGCTATGGCGCGCCAAGCAGCGCA
ACATCCCCGTCGTGTTGGGCTCGGCCACGCCGTCGCTGGAGACCTGGTGGCGCGCGGAGCAGACGCCCACCACGCGCCTG
ACGCTCTCGCGGCGCGCGCAGGCCGAAGCAGTGCTGCCGCGCGTGTCGCTGATCGACCTGAATCTGGAGCGCCGCGCCGG
GCGCCAGATTCGCGATGGGCTGTCCAAGCCACTTGTCGATGCGATTGCCGATCGTCTGGCGCGCGGCGAACAGAGCTTGC
TGTTCCTCAACCGCCGCGGCTACGCGCCCGTGCTCTCGTGCGACGCGTGCGGCTGGCTGTCGGGCTGTCCGCGCTGCTCG
GCGTACCTGGTGCTGCACAAGCCCGAGCGGCGCCTGCGCTGTCACCACTGCGGGTACGAATCGCGCATTCCGCATCACTG
CCCAGACTGCGGCAACGTCGACATCGCGCCGCTCGGGCGCGGCACCCAGCGCATCGAAGAGACGCTCGGCGAGCTGTTTC
CGCAGGCCCGCGTCGCCCGCATCGACGCCGATTCCACGCGCCGCAAGGGCAGCGCCGAAGCGCTGTTCGACACCGTGCAC
GAAGGCAGCGTCGACATCCTGATCGGCACGCAGATGGTCGCCAAGGGGCATGACTTCCAGCGCGTGACGCTGGTGGGCGT
GGTCGCGCCCGACCCCTCGCTGTTCTCGCACGACTTTCGCGCAGGCGAGCATCTCTTTGCATCGCTGATGCAGGTGGCGG
GGCGCGCGGGCCGGGCGGGGTTGGCGGGCGAGGTGCTGATTCAGACGCGGTATCCAGATGCTCCGGCGCTGCAGGCGCTG
GTTCGGCACGACTACGATGGGTTTGCGCGCCAATTGCTGCGGGAGCGCAAGCAGGCCGGGCTACCGCCGTTTGCCTATCA
GGCGCTGCTCACTGCCGAGCACAAGGAAGTCGCGCGGGCGCTTGAATTCTTGAGTGCTGCGCGCTCGGCAGGTGAAGCCT
TGGCTGCTGAATTGGGTGCGCCAGTGTTCTTGCATGACCCGGTGCCGATGACGATGGTGCGGCTGGCCAACCGCGAGCGG
GCGCAGTTGCTGGTGGAGTCGGGTTCTCGGGCGGCTTTGCAGAAGCTTTTGACTGCCTGGACTGAGGGGTTTGCGGGGAT
CGGCAAGACGGTGAAGGGGGTGCGGTGGCAGTTGGAGATTGATCCTTTGCGGATTTGA

Upstream 100 bases:

>100_bases
CTGTAAAGGCAAGGTGAGATGGGCAAGACGCATTTTCCAGGTGCAGGATCCGCCGCTGCATCGCAACATTCCCTTTGCAA
TTCAACCGTTTGCGCCTTGC

Downstream 100 bases:

>100_bases
GCGCTGGTGGTCTGCCCCCCTTTCGGTCCCGGCAGGGGATGAAACAAGGGATGCACCAAGAACAAAAACCCCCGTCCCCC
AACCCGTAAAGCCATCCCAA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 765; Mature: 764

Protein sequence:

>765_residues
MTTSQVDAAFSDAKVSASIARVVIDTPLDAVFDYRCGQPVLPGQLVVVPFGNRRVAGLVVETAATTDVPQEKLRDVERVL
DWVPPLDAEWRALAEFAAGYYHRALGEVTLPALPPLWRTPGSWDSLARQATETVYVMFEPARAALLDSIPKRASGAMRLA
EALTGDGELSTSAAVELHGQAVAKLRDWTSVGWLRAELRPKALLPQPLELPLPSIAPALNDEQTAAVAAIAQAGAAGNFS
PFLLYGVTGSGKTEVYLHAIADALAREPAAQVLMLVPEINLTPQLEARIAARFPSVPMAALHSGLAEQPRALNWLAAHRG
EARIVLGTRLAMLASLPKLALILVDEEHDTSYKQQEGLRYSARDLALWRAKQRNIPVVLGSATPSLETWWRAEQTPTTRL
TLSRRAQAEAVLPRVSLIDLNLERRAGRQIRDGLSKPLVDAIADRLARGEQSLLFLNRRGYAPVLSCDACGWLSGCPRCS
AYLVLHKPERRLRCHHCGYESRIPHHCPDCGNVDIAPLGRGTQRIEETLGELFPQARVARIDADSTRRKGSAEALFDTVH
EGSVDILIGTQMVAKGHDFQRVTLVGVVAPDPSLFSHDFRAGEHLFASLMQVAGRAGRAGLAGEVLIQTRYPDAPALQAL
VRHDYDGFARQLLRERKQAGLPPFAYQALLTAEHKEVARALEFLSAARSAGEALAAELGAPVFLHDPVPMTMVRLANRER
AQLLVESGSRAALQKLLTAWTEGFAGIGKTVKGVRWQLEIDPLRI

Sequences:

>Translated_765_residues
MTTSQVDAAFSDAKVSASIARVVIDTPLDAVFDYRCGQPVLPGQLVVVPFGNRRVAGLVVETAATTDVPQEKLRDVERVL
DWVPPLDAEWRALAEFAAGYYHRALGEVTLPALPPLWRTPGSWDSLARQATETVYVMFEPARAALLDSIPKRASGAMRLA
EALTGDGELSTSAAVELHGQAVAKLRDWTSVGWLRAELRPKALLPQPLELPLPSIAPALNDEQTAAVAAIAQAGAAGNFS
PFLLYGVTGSGKTEVYLHAIADALAREPAAQVLMLVPEINLTPQLEARIAARFPSVPMAALHSGLAEQPRALNWLAAHRG
EARIVLGTRLAMLASLPKLALILVDEEHDTSYKQQEGLRYSARDLALWRAKQRNIPVVLGSATPSLETWWRAEQTPTTRL
TLSRRAQAEAVLPRVSLIDLNLERRAGRQIRDGLSKPLVDAIADRLARGEQSLLFLNRRGYAPVLSCDACGWLSGCPRCS
AYLVLHKPERRLRCHHCGYESRIPHHCPDCGNVDIAPLGRGTQRIEETLGELFPQARVARIDADSTRRKGSAEALFDTVH
EGSVDILIGTQMVAKGHDFQRVTLVGVVAPDPSLFSHDFRAGEHLFASLMQVAGRAGRAGLAGEVLIQTRYPDAPALQAL
VRHDYDGFARQLLRERKQAGLPPFAYQALLTAEHKEVARALEFLSAARSAGEALAAELGAPVFLHDPVPMTMVRLANRER
AQLLVESGSRAALQKLLTAWTEGFAGIGKTVKGVRWQLEIDPLRI
>Mature_764_residues
TTSQVDAAFSDAKVSASIARVVIDTPLDAVFDYRCGQPVLPGQLVVVPFGNRRVAGLVVETAATTDVPQEKLRDVERVLD
WVPPLDAEWRALAEFAAGYYHRALGEVTLPALPPLWRTPGSWDSLARQATETVYVMFEPARAALLDSIPKRASGAMRLAE
ALTGDGELSTSAAVELHGQAVAKLRDWTSVGWLRAELRPKALLPQPLELPLPSIAPALNDEQTAAVAAIAQAGAAGNFSP
FLLYGVTGSGKTEVYLHAIADALAREPAAQVLMLVPEINLTPQLEARIAARFPSVPMAALHSGLAEQPRALNWLAAHRGE
ARIVLGTRLAMLASLPKLALILVDEEHDTSYKQQEGLRYSARDLALWRAKQRNIPVVLGSATPSLETWWRAEQTPTTRLT
LSRRAQAEAVLPRVSLIDLNLERRAGRQIRDGLSKPLVDAIADRLARGEQSLLFLNRRGYAPVLSCDACGWLSGCPRCSA
YLVLHKPERRLRCHHCGYESRIPHHCPDCGNVDIAPLGRGTQRIEETLGELFPQARVARIDADSTRRKGSAEALFDTVHE
GSVDILIGTQMVAKGHDFQRVTLVGVVAPDPSLFSHDFRAGEHLFASLMQVAGRAGRAGLAGEVLIQTRYPDAPALQALV
RHDYDGFARQLLRERKQAGLPPFAYQALLTAEHKEVARALEFLSAARSAGEALAAELGAPVFLHDPVPMTMVRLANRERA
QLLVESGSRAALQKLLTAWTEGFAGIGKTVKGVRWQLEIDPLRI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=768, Percent_Identity=39.7135416666667, Blast_Score=472, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 83279; Mature: 83147

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSQVDAAFSDAKVSASIARVVIDTPLDAVFDYRCGQPVLPGQLVVVPFGNRRVAGLVV
CCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEE
ETAATTDVPQEKLRDVERVLDWVPPLDAEWRALAEFAAGYYHRALGEVTLPALPPLWRTP
EECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GSWDSLARQATETVYVMFEPARAALLDSIPKRASGAMRLAEALTGDGELSTSAAVELHGQ
CCHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEEHHH
AVAKLRDWTSVGWLRAELRPKALLPQPLELPLPSIAPALNDEQTAAVAAIAQAGAAGNFS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
PFLLYGVTGSGKTEVYLHAIADALAREPAAQVLMLVPEINLTPQLEARIAARFPSVPMAA
CEEEEEECCCCCCHHHHHHHHHHHHCCCHHHEEEEECCCCCCCCHHHHHHHHCCCCCHHH
LHSGLAEQPRALNWLAAHRGEARIVLGTRLAMLASLPKLALILVDEEHDTSYKQQEGLRY
HHCCHHCCCCHHHHHHHCCCCEEEEEHHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCCC
SARDLALWRAKQRNIPVVLGSATPSLETWWRAEQTPTTRLTLSRRAQAEAVLPRVSLIDL
CHHHHHHHHHHCCCCCEEECCCCCCHHHHHCCCCCCCCEEEHHHHHHHHHHCCCEEEEEE
NLERRAGRQIRDGLSKPLVDAIADRLARGEQSLLFLNRRGYAPVLSCDACGWLSGCPRCS
CCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCCCCCCCE
AYLVLHKPERRLRCHHCGYESRIPHHCPDCGNVDIAPLGRGTQRIEETLGELFPQARVAR
EEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEEE
IDADSTRRKGSAEALFDTVHEGSVDILIGTQMVAKGHDFQRVTLVGVVAPDPSLFSHDFR
ECCCHHCCCCCHHHHHHHHCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCCCHHHHHHH
AGEHLFASLMQVAGRAGRAGLAGEVLIQTRYPDAPALQALVRHDYDGFARQLLRERKQAG
HHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
LPPFAYQALLTAEHKEVARALEFLSAARSAGEALAAELGAPVFLHDPVPMTMVRLANRER
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCHHH
AQLLVESGSRAALQKLLTAWTEGFAGIGKTVKGVRWQLEIDPLRI
HHHHHHCCCHHHHHHHHHHHHHHHHHHCHHCCCEEEEEEECCCCC
>Mature Secondary Structure 
TTSQVDAAFSDAKVSASIARVVIDTPLDAVFDYRCGQPVLPGQLVVVPFGNRRVAGLVV
CCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEE
ETAATTDVPQEKLRDVERVLDWVPPLDAEWRALAEFAAGYYHRALGEVTLPALPPLWRTP
EECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GSWDSLARQATETVYVMFEPARAALLDSIPKRASGAMRLAEALTGDGELSTSAAVELHGQ
CCHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEEHHH
AVAKLRDWTSVGWLRAELRPKALLPQPLELPLPSIAPALNDEQTAAVAAIAQAGAAGNFS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
PFLLYGVTGSGKTEVYLHAIADALAREPAAQVLMLVPEINLTPQLEARIAARFPSVPMAA
CEEEEEECCCCCCHHHHHHHHHHHHCCCHHHEEEEECCCCCCCCHHHHHHHHCCCCCHHH
LHSGLAEQPRALNWLAAHRGEARIVLGTRLAMLASLPKLALILVDEEHDTSYKQQEGLRY
HHCCHHCCCCHHHHHHHCCCCEEEEEHHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCCC
SARDLALWRAKQRNIPVVLGSATPSLETWWRAEQTPTTRLTLSRRAQAEAVLPRVSLIDL
CHHHHHHHHHHCCCCCEEECCCCCCHHHHHCCCCCCCCEEEHHHHHHHHHHCCCEEEEEE
NLERRAGRQIRDGLSKPLVDAIADRLARGEQSLLFLNRRGYAPVLSCDACGWLSGCPRCS
CCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCCCCCCCE
AYLVLHKPERRLRCHHCGYESRIPHHCPDCGNVDIAPLGRGTQRIEETLGELFPQARVAR
EEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEEE
IDADSTRRKGSAEALFDTVHEGSVDILIGTQMVAKGHDFQRVTLVGVVAPDPSLFSHDFR
ECCCHHCCCCCHHHHHHHHCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCCCHHHHHHH
AGEHLFASLMQVAGRAGRAGLAGEVLIQTRYPDAPALQALVRHDYDGFARQLLRERKQAG
HHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
LPPFAYQALLTAEHKEVARALEFLSAARSAGEALAAELGAPVFLHDPVPMTMVRLANRER
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCHHH
AQLLVESGSRAALQKLLTAWTEGFAGIGKTVKGVRWQLEIDPLRI
HHHHHHCCCHHHHHHHHHHHHHHHHHHCHHCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]