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The map label for this gene is pilT [H]

Identifier: 187929986

GI number: 187929986

Start: 3031777

End: 3032820

Strand: Reverse

Name: pilT [H]

Synonym: Rpic_2918

Alternate gene names: 187929986

Gene position: 3032820-3031777 (Counterclockwise)

Preceding gene: 187929989

Following gene: 187929985

Centisome position: 76.93

GC content: 61.59

Gene sequence:

>1044_bases
ATGGACATCGCGCAGCTTCTGGCGTTTTCCGCCAAGAACAAGGCGTCGGACTTGCACCTGTCGGCCGGACTGCCGCCGAT
GATCCGGATCCACGGCGACATGCGCCGGATCAACGTGCCCCCGCTCACGCACCAAGATGTGCACTCCATGGTGTACGACA
TCATGAGCGACGGGCAGCGCAAGGTCTACGAAGAAAACCTGGAAGTCGACTTCTCGTTTGAGATCCCGGGGCTCTCGCGT
TTCCGGGTCAATGCGTTCAACCAGAATCGCGGCGCTTCGGCCGTGTTCCGGACGATTCCGTCGAAGGTGCTCTCGCTGGA
CGACCTGAAAGCGCCGGCCGTGTTTGCCGATCTGGCGATGAAGCCGCGCGGTCTGGTGCTCGTCACGGGCCCGACCGGCT
CGGGCAAGTCGACGACGCTTGCCGCGATGGTCAACCACCGCAACGAAAACGACATGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCACGAATCGAAGAAGAGCCTGATCAACCAGCGTGAACTCGGGCCCCATACCCATTCGTTCGCCAA
CGCGCTGAAGTCGGCGCTGCGTGAAGACCCGGATGTGGTGCTGGTCGGCGAATTGCGTGACCTTGAAACCATTCGCCTCG
CGCTTACCGCCGCAGAAACCGGCCACTTGGTGTTCGGCACGCTGCACACCAGCTCGGCAGCCAAGACCATCGACCGGGTC
GTCGACGTGTTTCCGTCGGACGAAAAAGAGATGGTCCGGACCATGCTTTCCGAATCGCTGGAGGCGGTGATCTCGCAGAC
GCTGCTCAAGACGCGCGATGGTTCGGGCCGGATCGCGGCGCACGAAATCATGATTGCCACGCCGGCCATTCGTCACTTGA
TCCGCGAGAACAAGATCTCGCAGATGTACTCGATGATGCAGACCAGCAGCGGCATGGGCATGCAGACGCTCGACCAGTGC
CTGTCGGAGCTGATCAAGCGCTCGGCGATCAACTACGCAGACGCGCGCGCCATTGCCAAGAACCCAGACGCGTTCGTGAG
CTGA

Upstream 100 bases:

>100_bases
ATAAACAAGGTTCCGGCACGTCGAATGAACGGCTCGGAGCGCGTCTGGAGGCGCCATTGCGACGCCCGGGCGACATAAAA
AAGTCGGGGGTCATTATAAG

Downstream 100 bases:

>100_bases
TGCGCGAACGGATGCGCTGACTGACACGTCAGCGCGCTTTCCAATTCTTTCCAGGAGGGGCACGCCATGCTGGACCGCGA
AGCCGCCACCAAATACATCC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MDIAQLLAFSAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHSMVYDIMSDGQRKVYEENLEVDFSFEIPGLSR
FRVNAFNQNRGASAVFRTIPSKVLSLDDLKAPAVFADLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNENDMGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPSDEKEMVRTMLSESLEAVISQTLLKTRDGSGRIAAHEIMIATPAIRHLIRENKISQMYSMMQTSSGMGMQTLDQC
LSELIKRSAINYADARAIAKNPDAFVS

Sequences:

>Translated_347_residues
MDIAQLLAFSAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHSMVYDIMSDGQRKVYEENLEVDFSFEIPGLSR
FRVNAFNQNRGASAVFRTIPSKVLSLDDLKAPAVFADLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNENDMGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPSDEKEMVRTMLSESLEAVISQTLLKTRDGSGRIAAHEIMIATPAIRHLIRENKISQMYSMMQTSSGMGMQTLDQC
LSELIKRSAINYADARAIAKNPDAFVS
>Mature_347_residues
MDIAQLLAFSAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHSMVYDIMSDGQRKVYEENLEVDFSFEIPGLSR
FRVNAFNQNRGASAVFRTIPSKVLSLDDLKAPAVFADLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNENDMGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPSDEKEMVRTMLSESLEAVISQTLLKTRDGSGRIAAHEIMIATPAIRHLIRENKISQMYSMMQTSSGMGMQTLDQC
LSELIKRSAINYADARAIAKNPDAFVS

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=319, Percent_Identity=47.6489028213166, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1789723, Length=348, Percent_Identity=32.7586206896552, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786296, Length=313, Percent_Identity=32.5878594249201, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38195; Mature: 38195

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIAQLLAFSAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHSMVYDIMSDGQR
CCHHHHHHHHCCCCCCCEEECCCCCCEEEEECCEEECCCCCCCHHHHHHHHHHHHCCCHH
KVYEENLEVDFSFEIPGLSRFRVNAFNQNRGASAVFRTIPSKVLSLDDLKAPAVFADLAM
HHHHCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCC
KPRGLVLVTGPTGSGKSTTLAAMVNHRNENDMGHILTVEDPIEFVHESKKSLINQRELGP
CCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCC
HTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
CHHHHHHHHHHHHHCCCCEEEEECCCCHHHEEEEEEECCCCCEEEEEEECCHHHHHHHHH
VDVFPSDEKEMVRTMLSESLEAVISQTLLKTRDGSGRIAAHEIMIATPAIRHLIRENKIS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHEEEECHHHHHHHHHHHHH
QMYSMMQTSSGMGMQTLDQCLSELIKRSAINYADARAIAKNPDAFVS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDIAQLLAFSAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHSMVYDIMSDGQR
CCHHHHHHHHCCCCCCCEEECCCCCCEEEEECCEEECCCCCCCHHHHHHHHHHHHCCCHH
KVYEENLEVDFSFEIPGLSRFRVNAFNQNRGASAVFRTIPSKVLSLDDLKAPAVFADLAM
HHHHCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCC
KPRGLVLVTGPTGSGKSTTLAAMVNHRNENDMGHILTVEDPIEFVHESKKSLINQRELGP
CCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCC
HTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
CHHHHHHHHHHHHHCCCCEEEEECCCCHHHEEEEEEECCCCCEEEEEEECCHHHHHHHHH
VDVFPSDEKEMVRTMLSESLEAVISQTLLKTRDGSGRIAAHEIMIATPAIRHLIRENKIS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHEEEECHHHHHHHHHHHHH
QMYSMMQTSSGMGMQTLDQCLSELIKRSAINYADARAIAKNPDAFVS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]