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The map label for this gene is pilT [H]

Identifier: 187929985

GI number: 187929985

Start: 3030571

End: 3031710

Strand: Reverse

Name: pilT [H]

Synonym: Rpic_2917

Alternate gene names: 187929985

Gene position: 3031710-3030571 (Counterclockwise)

Preceding gene: 187929986

Following gene: 187929984

Centisome position: 76.9

GC content: 61.23

Gene sequence:

>1140_bases
ATGCTGGACCGCGAAGCCGCCACCAAATACATCCACGAACTCTTGCAGTTGATGGTGAACAGCCGTGGCTCGGACTTGTT
CATCACGGCAGAATTTCCGCCCGCGATCAAGGTGGACGGCAAGGTCACGCCGATCTCGCAGCAGCCGCTGAATGCCCAGC
AGGCCGTCGGTCTGGTCAAGGCGATCATGAACGAGAAGCAGCTGCGCGAGTACGAAGACAGCTCGGAATGCAACTTCGCC
ATCACGGCGCCGGGCGCCGGGCGATTCCGCGTGTCGGCGTTCATGCAGCAAGGTCGCGCCGGCATGGTGCTGCGGACCAT
CAACACGAAGATCCCGACGCTGGGCGAGTTGGACCTGCCGCCGATCCTCAATGAGATCGTGATGAGCAAGCGCGGCCTCG
TGATCGTCGTGGGCGCCACGGGCTCGGGTAAGTCGACCTCGCTGGCGGCGATGGTGGGCTATCGCAATGCGAATTCGTAC
GGCCACATCATCACCATTGAAGATCCGGTGGAATACGTGCACGCGCACCAGAACTGCGTGGTGACGCAGCGCGAGGTGGG
TGTGGATACCGAGTCGTGGCACGTGGCGCTGAAGAACACGCTGCGGCAGGCGCCGGACGTGATCCTGATCGGCGAAATCC
GGGATCGCGACACGATGGAATACGCCATCCAGTACGCGGAAACCGGCTACTTGTGCCTTGCCACGCTGCACGCCAACAGC
TCGAACCAGGCGATCGACCGGATCATCAACTTCTTCCCGGAAGAGAAGCGTCAGCAGCTGCTGATCGACCTGTCGCTGAA
CCTGCGCGCGATGATTGCGCAACGCCTGCTGCCGCGTCAGGGCAAGAAGGGGCGCGTACCGGCCGTCGAGATCATGCTGG
CGACCCCGCTGGTGCAAGACCTCATCTTCAAGGGCGAGGTGCACGAGCTGAAGGAGGTCATGAAGAAATCCCGCGAGCAA
GGGATGATCTCGTTCGACCAGGCGCTGTTCGATTTGTACGAAGAAAACAAGATCACGTACGAAGACGCGCTGCGTAATGC
CGACTCTCTCAACGATCTGCGGCTGCAGATCAAACTGCACAGCAAGCGGGGCGGGCAGACGGACCTGTCGGCCGGCACCG
AGCATTTGAATGTCGTCTGA

Upstream 100 bases:

>100_bases
CATTGCCAAGAACCCAGACGCGTTCGTGAGCTGATGCGCGAACGGATGCGCTGACTGACACGTCAGCGCGCTTTCCAATT
CTTTCCAGGAGGGGCACGCC

Downstream 100 bases:

>100_bases
TGTGGTCCAGGCGCTACACCATTTGAGGTAATTTGCGGCGCGCAACGTTTGCGCTTCGCCCTGTACCCACCCGTCGTCAT
TAACTTTTCTTAATATTTGC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MLDREAATKYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLNAQQAVGLVKAIMNEKQLREYEDSSECNFA
ITAPGAGRFRVSAFMQQGRAGMVLRTINTKIPTLGELDLPPILNEIVMSKRGLVIVVGATGSGKSTSLAAMVGYRNANSY
GHIITIEDPVEYVHAHQNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGYLCLATLHANS
SNQAIDRIINFFPEEKRQQLLIDLSLNLRAMIAQRLLPRQGKKGRVPAVEIMLATPLVQDLIFKGEVHELKEVMKKSREQ
GMISFDQALFDLYEENKITYEDALRNADSLNDLRLQIKLHSKRGGQTDLSAGTEHLNVV

Sequences:

>Translated_379_residues
MLDREAATKYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLNAQQAVGLVKAIMNEKQLREYEDSSECNFA
ITAPGAGRFRVSAFMQQGRAGMVLRTINTKIPTLGELDLPPILNEIVMSKRGLVIVVGATGSGKSTSLAAMVGYRNANSY
GHIITIEDPVEYVHAHQNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGYLCLATLHANS
SNQAIDRIINFFPEEKRQQLLIDLSLNLRAMIAQRLLPRQGKKGRVPAVEIMLATPLVQDLIFKGEVHELKEVMKKSREQ
GMISFDQALFDLYEENKITYEDALRNADSLNDLRLQIKLHSKRGGQTDLSAGTEHLNVV
>Mature_379_residues
MLDREAATKYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLNAQQAVGLVKAIMNEKQLREYEDSSECNFA
ITAPGAGRFRVSAFMQQGRAGMVLRTINTKIPTLGELDLPPILNEIVMSKRGLVIVVGATGSGKSTSLAAMVGYRNANSY
GHIITIEDPVEYVHAHQNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGYLCLATLHANS
SNQAIDRIINFFPEEKRQQLLIDLSLNLRAMIAQRLLPRQGKKGRVPAVEIMLATPLVQDLIFKGEVHELKEVMKKSREQ
GMISFDQALFDLYEENKITYEDALRNADSLNDLRLQIKLHSKRGGQTDLSAGTEHLNVV

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=318, Percent_Identity=36.7924528301887, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1789723, Length=163, Percent_Identity=39.2638036809816, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786296, Length=378, Percent_Identity=27.2486772486773, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 42251; Mature: 42251

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDREAATKYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLNAQQAVGLVK
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEECCEECCCCCCCCCHHHHHHHHH
AIMNEKQLREYEDSSECNFAITAPGAGRFRVSAFMQQGRAGMVLRTINTKIPTLGELDLP
HHHCHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
PILNEIVMSKRGLVIVVGATGSGKSTSLAAMVGYRNANSYGHIITIEDPVEYVHAHQNCV
HHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCEEEEECCHHHHHHHCCCEE
VTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGYLCLATLHANS
EEHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCC
SNQAIDRIINFFPEEKRQQLLIDLSLNLRAMIAQRLLPRQGKKGRVPAVEIMLATPLVQD
CHHHHHHHHHHCCCCCHHEEEEEECCCHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHH
LIFKGEVHELKEVMKKSREQGMISFDQALFDLYEENKITYEDALRNADSLNDLRLQIKLH
HHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEEEEE
SKRGGQTDLSAGTEHLNVV
CCCCCCCCHHCCCCCCCCC
>Mature Secondary Structure
MLDREAATKYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLNAQQAVGLVK
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEECCEECCCCCCCCCHHHHHHHHH
AIMNEKQLREYEDSSECNFAITAPGAGRFRVSAFMQQGRAGMVLRTINTKIPTLGELDLP
HHHCHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
PILNEIVMSKRGLVIVVGATGSGKSTSLAAMVGYRNANSYGHIITIEDPVEYVHAHQNCV
HHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCEEEEECCHHHHHHHCCCEE
VTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGYLCLATLHANS
EEHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCC
SNQAIDRIINFFPEEKRQQLLIDLSLNLRAMIAQRLLPRQGKKGRVPAVEIMLATPLVQD
CHHHHHHHHHHCCCCCHHEEEEEECCCHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHH
LIFKGEVHELKEVMKKSREQGMISFDQALFDLYEENKITYEDALRNADSLNDLRLQIKLH
HHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEEEEE
SKRGGQTDLSAGTEHLNVV
CCCCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]