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The map label for this gene is pcaB [H]

Identifier: 187929535

GI number: 187929535

Start: 2565906

End: 2567267

Strand: Reverse

Name: pcaB [H]

Synonym: Rpic_2456

Alternate gene names: 187929535

Gene position: 2567267-2565906 (Counterclockwise)

Preceding gene: 187929536

Following gene: 187929534

Centisome position: 65.12

GC content: 67.25

Gene sequence:

>1362_bases
ATGACAAGCGCATTGCTCGATCGCGCCTTCTCCACGCCGGCCATGCTGGCGGTGTGGTCCGATGCGGCCACGGTGCGCGC
AATGCTCGATGTGGAGGCCGCGCTTGCGCAGGCCGAAGGCGCGGTTGGCGTGATTCCGCCCGAAGCCGTCGCGCCGATTC
GCGCGGTGTGCGCCAAGGCGACGTTGGATCTGGACGCGCTGGGCAACGCTGCTGCCACCGCAGGCAACCTGGCGATTCCC
CTGGTCAAGCAACTGACTGCGGCCGTCGCACAGCATGATGAAAACGCCGCGCGCTACGTGCATTGGGGCGCGACCAGCCA
GGACATCATCGATACCGGTCTGATGCTGCAGTGGCGGCTATCGGCCGAACTGATCGAAGCGGATCTGCAGAAGCTGGCTG
ACGCGCTCGCGGCACTCGCGCAACGCCATCGCAATACGCCGATGGTGGCGCGCACGTGGTTGCAGCAGGCGCTGCCGACC
ACGTTCGGCCGCAAGGTTGCGGGTTGGCTGGAAGCGGTGCATCGCGCGCAGGATCGCTTTACCGCGCTGAGCGCGCATGT
GCCTGCGTTGCAGTTTGGCGGGGCGGCAGGCACGCTTGCCAGCCTGGGCGAACACGCACGGGCCGTCGCTCAAACGCTGG
CGCACGAGCTTGATCTGCCGCTGCCGGCGCTCTCGTGGCACGGCGAGCAGGATCGAATCGCCGAGATCGGCACGACGCTC
GCGTTGCTGACCGGCACACTGGGCCACTTCGCGCGTGATCTCTCGCTGATGATGCAGACAGAGGTGGGCGAGGTTTCCGA
GCCTTCCGGTGCAGGCAAGGGTGGTTCTTCCACCATGCCGCACAAACGCAACCCGGTCGGGTGCGCGACGGTGCTGGCGG
CGGCCACGCGCATGCCTGGCTTGGCGAGCACGCTGCTCGCTGCGCTACCGCAGGAGCACGAGCGCGCGCTCGGCGGTTGG
CAGGCGCAGTGGGCGACGTTGCGCGAGATGGCCTGCCTCGCGGCGGGCGCGCTCGATCGCATGCGCGACATCGTTGAAGG
TTTGCAGGTCGACGCTGCTCGCATGCGCACGAATCTGGACCTTACGCACGGCCTGATCCTTGGTGAAGCCGTGATGCTGG
CGCTGGGTGCGGATCTTGGGCGTCTGCAAGCGCACCATGTGGTCGAAGCTGCCAGCCGCCAAGCGGTACAAGAGAACAAG
ACGCTGCATGACGTACTGGCGCAAGACGCAGAGGTCCTTCGCATCTGGGGCGCGACCGCGCCGCAACGGTTGAGCGCGCT
GCTCGATCCCACCCATTACCTTGGCGATGCGGGCGCCGCTGTTGACCGCGTGCTCGCAGAACACGCCCACCGCCACCCCT
GA

Upstream 100 bases:

>100_bases
TATCAGTTGCAACGCACGGGCGGACGTTATGCGCTGTGCACGATGTGCATCGGCGTGGGCCAGGGCATTGCGCTGGTGAT
CGAACGCGTTTGACACGGCC

Downstream 100 bases:

>100_bases
ACACGTATTCCACGGAGACACCATGCCCTGGCTTGAACACGATAACGTCCGCCTGTATCACGAATTCGATGACGGCCCCG
CCACGGGTAAGCCGGTGCTG

Product: 3-carboxy-cis,cis-muconate cycloisomerase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MTSALLDRAFSTPAMLAVWSDAATVRAMLDVEAALAQAEGAVGVIPPEAVAPIRAVCAKATLDLDALGNAAATAGNLAIP
LVKQLTAAVAQHDENAARYVHWGATSQDIIDTGLMLQWRLSAELIEADLQKLADALAALAQRHRNTPMVARTWLQQALPT
TFGRKVAGWLEAVHRAQDRFTALSAHVPALQFGGAAGTLASLGEHARAVAQTLAHELDLPLPALSWHGEQDRIAEIGTTL
ALLTGTLGHFARDLSLMMQTEVGEVSEPSGAGKGGSSTMPHKRNPVGCATVLAAATRMPGLASTLLAALPQEHERALGGW
QAQWATLREMACLAAGALDRMRDIVEGLQVDAARMRTNLDLTHGLILGEAVMLALGADLGRLQAHHVVEAASRQAVQENK
TLHDVLAQDAEVLRIWGATAPQRLSALLDPTHYLGDAGAAVDRVLAEHAHRHP

Sequences:

>Translated_453_residues
MTSALLDRAFSTPAMLAVWSDAATVRAMLDVEAALAQAEGAVGVIPPEAVAPIRAVCAKATLDLDALGNAAATAGNLAIP
LVKQLTAAVAQHDENAARYVHWGATSQDIIDTGLMLQWRLSAELIEADLQKLADALAALAQRHRNTPMVARTWLQQALPT
TFGRKVAGWLEAVHRAQDRFTALSAHVPALQFGGAAGTLASLGEHARAVAQTLAHELDLPLPALSWHGEQDRIAEIGTTL
ALLTGTLGHFARDLSLMMQTEVGEVSEPSGAGKGGSSTMPHKRNPVGCATVLAAATRMPGLASTLLAALPQEHERALGGW
QAQWATLREMACLAAGALDRMRDIVEGLQVDAARMRTNLDLTHGLILGEAVMLALGADLGRLQAHHVVEAASRQAVQENK
TLHDVLAQDAEVLRIWGATAPQRLSALLDPTHYLGDAGAAVDRVLAEHAHRHP
>Mature_452_residues
TSALLDRAFSTPAMLAVWSDAATVRAMLDVEAALAQAEGAVGVIPPEAVAPIRAVCAKATLDLDALGNAAATAGNLAIPL
VKQLTAAVAQHDENAARYVHWGATSQDIIDTGLMLQWRLSAELIEADLQKLADALAALAQRHRNTPMVARTWLQQALPTT
FGRKVAGWLEAVHRAQDRFTALSAHVPALQFGGAAGTLASLGEHARAVAQTLAHELDLPLPALSWHGEQDRIAEIGTTLA
LLTGTLGHFARDLSLMMQTEVGEVSEPSGAGKGGSSTMPHKRNPVGCATVLAAATRMPGLASTLLAALPQEHERALGGWQ
AQWATLREMACLAAGALDRMRDIVEGLQVDAARMRTNLDLTHGLILGEAVMLALGADLGRLQAHHVVEAASRQAVQENKT
LHDVLAQDAEVLRIWGATAPQRLSALLDPTHYLGDAGAAVDRVLAEHAHRHP

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323391, Length=374, Percent_Identity=23.5294117647059, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 47814; Mature: 47683

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSALLDRAFSTPAMLAVWSDAATVRAMLDVEAALAQAEGAVGVIPPEAVAPIRAVCAKA
CCHHHHHHHCCCCHHEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
TLDLDALGNAAATAGNLAIPLVKQLTAAVAQHDENAARYVHWGATSQDIIDTGLMLQWRL
HCCHHHCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCEEEEHH
SAELIEADLQKLADALAALAQRHRNTPMVARTWLQQALPTTFGRKVAGWLEAVHRAQDRF
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TALSAHVPALQFGGAAGTLASLGEHARAVAQTLAHELDLPLPALSWHGEQDRIAEIGTTL
HHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ALLTGTLGHFARDLSLMMQTEVGEVSEPSGAGKGGSSTMPHKRNPVGCATVLAAATRMPG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
LASTLLAALPQEHERALGGWQAQWATLREMACLAAGALDRMRDIVEGLQVDAARMRTNLD
HHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LTHGLILGEAVMLALGADLGRLQAHHVVEAASRQAVQENKTLHDVLAQDAEVLRIWGATA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHCCCC
PQRLSALLDPTHYLGDAGAAVDRVLAEHAHRHP
HHHHHHHHCHHHHHCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSALLDRAFSTPAMLAVWSDAATVRAMLDVEAALAQAEGAVGVIPPEAVAPIRAVCAKA
CHHHHHHHCCCCHHEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
TLDLDALGNAAATAGNLAIPLVKQLTAAVAQHDENAARYVHWGATSQDIIDTGLMLQWRL
HCCHHHCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCEEEEHH
SAELIEADLQKLADALAALAQRHRNTPMVARTWLQQALPTTFGRKVAGWLEAVHRAQDRF
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TALSAHVPALQFGGAAGTLASLGEHARAVAQTLAHELDLPLPALSWHGEQDRIAEIGTTL
HHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ALLTGTLGHFARDLSLMMQTEVGEVSEPSGAGKGGSSTMPHKRNPVGCATVLAAATRMPG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
LASTLLAALPQEHERALGGWQAQWATLREMACLAAGALDRMRDIVEGLQVDAARMRTNLD
HHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LTHGLILGEAVMLALGADLGRLQAHHVVEAASRQAVQENKTLHDVLAQDAEVLRIWGATA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHCCCC
PQRLSALLDPTHYLGDAGAAVDRVLAEHAHRHP
HHHHHHHHCHHHHHCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9582432; 12597275 [H]