Click here to switch to the map view.

The map label for this gene is pcaF [H]

Identifier: 187929536

GI number: 187929536

Start: 2567275

End: 2568477

Strand: Reverse

Name: pcaF [H]

Synonym: Rpic_2457

Alternate gene names: 187929536

Gene position: 2568477-2567275 (Counterclockwise)

Preceding gene: 187929537

Following gene: 187929535

Centisome position: 65.15

GC content: 67.17

Gene sequence:

>1203_bases
ATGACCGAAGCCTTTATCTGCGACGCCATCCGGACGCCCATCGGCCGCTACGGCGGCAGCCTGTCCGCCGTGCGCGCCGA
TGACCTCGGCGCGGTGCCGCTCAAGGCGCTGATGGCGCGCAACCCGCAGGTCGACTGGTCGGCCATCGACGACGTGATCT
TCGGCTGCGCCAACCAGGCGGGCGAAGACAACCGCAACGTGGCGCGCATGTCGTCGTTGCTGGCTGGCTTGCCCGATAGC
GTGCCGGGCTCGACCATCAACCGCTTGTGCGGCTCGGGCATGGACGCCACCGGCACCGCGGCGCGCGCTATTCGCTCAGG
TGAAACCGCGCTGATGATCGCCGGGGGCGTGGAGAGTATGAGCCGCGCGCCGTTCGTGATGGGCAAGGCTGCCAGCGCCT
TCTCGCGCGACGCTGCCATCTACGACACCACCATCGGCTGGCGCTTCATCAACCCGCTGATGAAGGCGCAATACGGTGTC
GACTCGATGCCCGAAACCGCCGAGAACGTCGCCACCGACTACAAGGTCAACCGAGAAGATCAGGACCGCTTCGCGCTGCG
CAGCCAGGCCAGCGCCGCGCGAGCCCAGGAAGACGGCACGCTCGCGCAGGAAATCACGCCGGTGACGATCCCGCAGAGGA
AGGGCGACGCTATCGTGGTGGACCGCGATGAGCATCCGCGTGCGACCAGCATGGAAGCGCTCGCCAAGCTCAAGGGCGTA
GTGCGCCCGGACGGCACCGTTACGGCCGGCAATGCTTCGGGCGTGAACGATGGCGCGTGCGCGCTGCTCCTCGCCAATGA
AGCGTCCGCCAAGCGCTTTGGCCTGACCCCGCGTGCGCGCATTGTCGGCATGGCCACCGCAGGCGTGCCGCCGCGCGTGA
TGGGCATTGGCCCGGCACCGGCGTCGCAGAAGCTGCTCAAGCAGCTCGGCTTGAGCATCGATCAGATGGATGTGATCGAG
CTGAACGAGGCATTTGCCGCGCAGGGTCTAGCGGTGACGCGTCAACTGGGCCTGCGTGATGACGACCCGCGCGTCAACCC
GAACGGCGGCGCCATCGCACTTGGCCACCCGCTCGGCATGAGCGGTGCGCGCCTGGTTACGACGGCGATGTATCAGTTGC
AACGCACGGGCGGACGTTATGCGCTGTGCACGATGTGCATCGGCGTGGGCCAGGGCATTGCGCTGGTGATCGAACGCGTT
TGA

Upstream 100 bases:

>100_bases
GTGGTGCGCGAGATGGCCGACGGCCTCGATTTCGATACGCTGCAGACGATGACGGCGGCGCCGTTGCGTCGTGCCTAGAA
TTAGTGGAGACAGAACAATC

Downstream 100 bases:

>100_bases
CACGGCCATGACAAGCGCATTGCTCGATCGCGCCTTCTCCACGCCGGCCATGCTGGCGGTGTGGTCCGATGCGGCCACGG
TGCGCGCAATGCTCGATGTG

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTEAFICDAIRTPIGRYGGSLSAVRADDLGAVPLKALMARNPQVDWSAIDDVIFGCANQAGEDNRNVARMSSLLAGLPDS
VPGSTINRLCGSGMDATGTAARAIRSGETALMIAGGVESMSRAPFVMGKAASAFSRDAAIYDTTIGWRFINPLMKAQYGV
DSMPETAENVATDYKVNREDQDRFALRSQASAARAQEDGTLAQEITPVTIPQRKGDAIVVDRDEHPRATSMEALAKLKGV
VRPDGTVTAGNASGVNDGACALLLANEASAKRFGLTPRARIVGMATAGVPPRVMGIGPAPASQKLLKQLGLSIDQMDVIE
LNEAFAAQGLAVTRQLGLRDDDPRVNPNGGAIALGHPLGMSGARLVTTAMYQLQRTGGRYALCTMCIGVGQGIALVIERV

Sequences:

>Translated_400_residues
MTEAFICDAIRTPIGRYGGSLSAVRADDLGAVPLKALMARNPQVDWSAIDDVIFGCANQAGEDNRNVARMSSLLAGLPDS
VPGSTINRLCGSGMDATGTAARAIRSGETALMIAGGVESMSRAPFVMGKAASAFSRDAAIYDTTIGWRFINPLMKAQYGV
DSMPETAENVATDYKVNREDQDRFALRSQASAARAQEDGTLAQEITPVTIPQRKGDAIVVDRDEHPRATSMEALAKLKGV
VRPDGTVTAGNASGVNDGACALLLANEASAKRFGLTPRARIVGMATAGVPPRVMGIGPAPASQKLLKQLGLSIDQMDVIE
LNEAFAAQGLAVTRQLGLRDDDPRVNPNGGAIALGHPLGMSGARLVTTAMYQLQRTGGRYALCTMCIGVGQGIALVIERV
>Mature_399_residues
TEAFICDAIRTPIGRYGGSLSAVRADDLGAVPLKALMARNPQVDWSAIDDVIFGCANQAGEDNRNVARMSSLLAGLPDSV
PGSTINRLCGSGMDATGTAARAIRSGETALMIAGGVESMSRAPFVMGKAASAFSRDAAIYDTTIGWRFINPLMKAQYGVD
SMPETAENVATDYKVNREDQDRFALRSQASAARAQEDGTLAQEITPVTIPQRKGDAIVVDRDEHPRATSMEALAKLKGVV
RPDGTVTAGNASGVNDGACALLLANEASAKRFGLTPRARIVGMATAGVPPRVMGIGPAPASQKLLKQLGLSIDQMDVIEL
NEAFAAQGLAVTRQLGLRDDDPRVNPNGGAIALGHPLGMSGARLVTTAMYQLQRTGGRYALCTMCIGVGQGIALVIERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=397, Percent_Identity=42.3173803526448, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=40.5541561712846, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI4501853, Length=398, Percent_Identity=40.4522613065327, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI4557237, Length=406, Percent_Identity=35.9605911330049, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=32.3185011709602, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI194353979, Length=394, Percent_Identity=30.2030456852792, Blast_Score=129, Evalue=4e-30,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=69.3266832917706, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=46.8982630272953, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=43.9506172839506, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=42.8927680798005, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1788683, Length=424, Percent_Identity=31.3679245283019, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=44.8877805486284, Blast_Score=315, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI25147385, Length=404, Percent_Identity=34.6534653465347, Blast_Score=227, Evalue=7e-60,
Organism=Caenorhabditis elegans, GI17535921, Length=406, Percent_Identity=33.743842364532, Blast_Score=200, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI17551802, Length=436, Percent_Identity=30.7339449541284, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=30.1234567901235, Blast_Score=154, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6325229, Length=404, Percent_Identity=38.1188118811881, Blast_Score=235, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6322031, Length=412, Percent_Identity=34.7087378640777, Blast_Score=213, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=44.3890274314214, Blast_Score=319, Evalue=2e-87,
Organism=Drosophila melanogaster, GI17648125, Length=406, Percent_Identity=39.9014778325123, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24640423, Length=406, Percent_Identity=34.2364532019704, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=31.8396226415094, Blast_Score=183, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41899; Mature: 41768

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAFICDAIRTPIGRYGGSLSAVRADDLGAVPLKALMARNPQVDWSAIDDVIFGCANQA
CCCHHHHHHHHCCHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
GEDNRNVARMSSLLAGLPDSVPGSTINRLCGSGMDATGTAARAIRSGETALMIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHCCCEEEEEECCHHHH
SRAPFVMGKAASAFSRDAAIYDTTIGWRFINPLMKAQYGVDSMPETAENVATDYKVNRED
HCCCEEECCHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCC
QDRFALRSQASAARAQEDGTLAQEITPVTIPQRKGDAIVVDRDEHPRATSMEALAKLKGV
HHHHHHHHHHHHHHHHCCCCHHHHCCCEECCCCCCCEEEEECCCCCCHHHHHHHHHHHCC
VRPDGTVTAGNASGVNDGACALLLANEASAKRFGLTPRARIVGMATAGVPPRVMGIGPAP
CCCCCCEECCCCCCCCCCEEEEEEECCCCCHHCCCCCCCEEEEEECCCCCCCEEECCCCC
ASQKLLKQLGLSIDQMDVIELNEAFAAQGLAVTRQLGLRDDDPRVNPNGGAIALGHPLGM
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCEEEECCCCCC
SGARLVTTAMYQLQRTGGRYALCTMCIGVGQGIALVIERV
CCHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure 
TEAFICDAIRTPIGRYGGSLSAVRADDLGAVPLKALMARNPQVDWSAIDDVIFGCANQA
CCHHHHHHHHCCHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
GEDNRNVARMSSLLAGLPDSVPGSTINRLCGSGMDATGTAARAIRSGETALMIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHCCCEEEEEECCHHHH
SRAPFVMGKAASAFSRDAAIYDTTIGWRFINPLMKAQYGVDSMPETAENVATDYKVNRED
HCCCEEECCHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCC
QDRFALRSQASAARAQEDGTLAQEITPVTIPQRKGDAIVVDRDEHPRATSMEALAKLKGV
HHHHHHHHHHHHHHHHCCCCHHHHCCCEECCCCCCCEEEEECCCCCCHHHHHHHHHHHCC
VRPDGTVTAGNASGVNDGACALLLANEASAKRFGLTPRARIVGMATAGVPPRVMGIGPAP
CCCCCCEECCCCCCCCCCEEEEEEECCCCCHHCCCCCCCEEEEEECCCCCCCEEECCCCC
ASQKLLKQLGLSIDQMDVIELNEAFAAQGLAVTRQLGLRDDDPRVNPNGGAIALGHPLGM
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCEEEECCCCCC
SGARLVTTAMYQLQRTGGRYALCTMCIGVGQGIALVIERV
CCHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11741863 [H]