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The map label for this gene is livK [C]

Identifier: 187929300

GI number: 187929300

Start: 2331269

End: 2332528

Strand: Reverse

Name: livK [C]

Synonym: Rpic_2221

Alternate gene names: 187929300

Gene position: 2332528-2331269 (Counterclockwise)

Preceding gene: 187929302

Following gene: 187929299

Centisome position: 59.16

GC content: 63.17

Gene sequence:

>1260_bases
ATGGTGTTTCGTTGGTTTGTGACCGCGCTGGCGGCTGTGGGGTTGTGCGGCGCGGCAAGTGCCCAGCCCGAAGGCGCGCC
GATCCGGCTGGCCCTGATTGAAGGGCTTTCGGGCCCGTTTGCCAATGCGGGCGAGGCAGTGGAGCGCAATCTGCGCATGG
CCATCGAAGGCGTAAATGCACGCGGTGGTGTGAAGCTGCCTGATGGCCACCATCCGCTTGCGCTTGTGCGCTTCGATAGC
AAGAACACCGTCGACGAATCGTTGCTGCAACTGCAGGCCGCCACGGATGCGGGTATCCGCTTCGTGCTGCAGGGCAACAG
TTCTGCGGCAGCGGCTGCATTGTCGACGTCGATCTCGCGCTACAACGACCGCCATCCGGATGCGCGCGTGCTGTTCCTGA
ACTACGCTGCCGTCGATCCGGCGCTGACCAATGAGGGATGCAGCTTCTGGCATTTCCGCTTCGATGCGTCGGCCGACATG
CGCATGCACGCGCTGACGGAAGTGATCCGCGATGACAAGGCGATCAAAAGGGTCTACCTGATTGGGCAGGACTACACGTT
CGGCCGCCAGGTGGCGCAATCGGCGCGCCAGCAGTTGCGCGAAAAGCGACCGGACATCGCGATCGTTGGCGAGGTGCTGC
ACCCGATCGGCAAGGTGAAGGACTTTGCGCCGTATGTGGCGCGTATTGCCGCGTCGGGTGCGGATACGGTCATCACCGGC
AACTGGGGGAACGATCTGACGCTGCTCGTCAAGGCCGCGCGAGATGCCGGGCTCAAGGCGCGCTTCTTTACCTTTTATGG
CAACTCGCTTGGTGCACCAGGCGCGATGGGCGAAGCCGGCGTCGGCCGTGTGGTGGCCGTGGCGGAGTGGCATCCGAACC
TGGGCGGTGCCAAGTCCGATGCGTTCTACAAGGCCTTCCGTGCGCGTTATCCGGCACCCGCCGACGATTACCCATTGCTG
CGCGACCAGATGTTGATCGACATGCTGGCCGCCGCCATCGAGCGTGCCGAGACCACGGAAGCTGCCGCCGTGGCGCGTGC
GCTAGAACGCGCATCGTTCGACAACGGCTTCCACCGCATTACGCTGCGAGCGGAGGACCATCAAGCCATTCAGCCGCTCG
TCGTCGCGCAGATGGAGCGGGCGGGCATGCCCGGTGCGCTGTTTGATATCGAGGGCTCGGGCTATGGTTTTCGTACGCTA
AAAGTGATTCAAGGGGCGCAAACCGCATTGCCCACGACCTGCAAAATGGTGCGCTATTAA

Upstream 100 bases:

>100_bases
CGATGCCTTCAGTGTAGGAGGCCGTGCGGTCTCTGCCGGCTTGTGGGCTTGTGCCGACATGCGGCGGCGCTTTACCATCC
GCGCTTCACGGAGATTTGCC

Downstream 100 bases:

>100_bases
GCAGGGCGGCACAGCACTGTCGTCATGGCGCGGTTCTGCGCTATAATCGCGGTCTTGCTGTCCAGACCGATTTTGGATGG
GCAGCAAGATCATTGTTCAA

Product: putative transporter signal peptide protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MVFRWFVTALAAVGLCGAASAQPEGAPIRLALIEGLSGPFANAGEAVERNLRMAIEGVNARGGVKLPDGHHPLALVRFDS
KNTVDESLLQLQAATDAGIRFVLQGNSSAAAAALSTSISRYNDRHPDARVLFLNYAAVDPALTNEGCSFWHFRFDASADM
RMHALTEVIRDDKAIKRVYLIGQDYTFGRQVAQSARQQLREKRPDIAIVGEVLHPIGKVKDFAPYVARIAASGADTVITG
NWGNDLTLLVKAARDAGLKARFFTFYGNSLGAPGAMGEAGVGRVVAVAEWHPNLGGAKSDAFYKAFRARYPAPADDYPLL
RDQMLIDMLAAAIERAETTEAAAVARALERASFDNGFHRITLRAEDHQAIQPLVVAQMERAGMPGALFDIEGSGYGFRTL
KVIQGAQTALPTTCKMVRY

Sequences:

>Translated_419_residues
MVFRWFVTALAAVGLCGAASAQPEGAPIRLALIEGLSGPFANAGEAVERNLRMAIEGVNARGGVKLPDGHHPLALVRFDS
KNTVDESLLQLQAATDAGIRFVLQGNSSAAAAALSTSISRYNDRHPDARVLFLNYAAVDPALTNEGCSFWHFRFDASADM
RMHALTEVIRDDKAIKRVYLIGQDYTFGRQVAQSARQQLREKRPDIAIVGEVLHPIGKVKDFAPYVARIAASGADTVITG
NWGNDLTLLVKAARDAGLKARFFTFYGNSLGAPGAMGEAGVGRVVAVAEWHPNLGGAKSDAFYKAFRARYPAPADDYPLL
RDQMLIDMLAAAIERAETTEAAAVARALERASFDNGFHRITLRAEDHQAIQPLVVAQMERAGMPGALFDIEGSGYGFRTL
KVIQGAQTALPTTCKMVRY
>Mature_419_residues
MVFRWFVTALAAVGLCGAASAQPEGAPIRLALIEGLSGPFANAGEAVERNLRMAIEGVNARGGVKLPDGHHPLALVRFDS
KNTVDESLLQLQAATDAGIRFVLQGNSSAAAAALSTSISRYNDRHPDARVLFLNYAAVDPALTNEGCSFWHFRFDASADM
RMHALTEVIRDDKAIKRVYLIGQDYTFGRQVAQSARQQLREKRPDIAIVGEVLHPIGKVKDFAPYVARIAASGADTVITG
NWGNDLTLLVKAARDAGLKARFFTFYGNSLGAPGAMGEAGVGRVVAVAEWHPNLGGAKSDAFYKAFRARYPAPADDYPLL
RDQMLIDMLAAAIERAETTEAAAVARALERASFDNGFHRITLRAEDHQAIQPLVVAQMERAGMPGALFDIEGSGYGFRTL
KVIQGAQTALPTTCKMVRY

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=324, Percent_Identity=22.2222222222222, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI48994941, Length=236, Percent_Identity=22.4576271186441, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 45203; Mature: 45203

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFRWFVTALAAVGLCGAASAQPEGAPIRLALIEGLSGPFANAGEAVERNLRMAIEGVNA
CCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHEEECCCC
RGGVKLPDGHHPLALVRFDSKNTVDESLLQLQAATDAGIRFVLQGNSSAAAAALSTSISR
CCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHH
YNDRHPDARVLFLNYAAVDPALTNEGCSFWHFRFDASADMRMHALTEVIRDDKAIKRVYL
HCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHEEEEEE
IGQDYTFGRQVAQSARQQLREKRPDIAIVGEVLHPIGKVKDFAPYVARIAASGADTVITG
ECCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
NWGNDLTLLVKAARDAGLKARFFTFYGNSLGAPGAMGEAGVGRVVAVAEWHPNLGGAKSD
CCCCCEEEEEEEHHCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHH
AFYKAFRARYPAPADDYPLLRDQMLIDMLAAAIERAETTEAAAVARALERASFDNGFHRI
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
TLRAEDHQAIQPLVVAQMERAGMPGALFDIEGSGYGFRTLKVIQGAQTALPTTCKMVRY
EEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHCC
>Mature Secondary Structure
MVFRWFVTALAAVGLCGAASAQPEGAPIRLALIEGLSGPFANAGEAVERNLRMAIEGVNA
CCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHEEECCCC
RGGVKLPDGHHPLALVRFDSKNTVDESLLQLQAATDAGIRFVLQGNSSAAAAALSTSISR
CCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHH
YNDRHPDARVLFLNYAAVDPALTNEGCSFWHFRFDASADMRMHALTEVIRDDKAIKRVYL
HCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHEEEEEE
IGQDYTFGRQVAQSARQQLREKRPDIAIVGEVLHPIGKVKDFAPYVARIAASGADTVITG
ECCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
NWGNDLTLLVKAARDAGLKARFFTFYGNSLGAPGAMGEAGVGRVVAVAEWHPNLGGAKSD
CCCCCEEEEEEEHHCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHH
AFYKAFRARYPAPADDYPLLRDQMLIDMLAAAIERAETTEAAAVARALERASFDNGFHRI
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
TLRAEDHQAIQPLVVAQMERAGMPGALFDIEGSGYGFRTLKVIQGAQTALPTTCKMVRY
EEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA