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The map label for this gene is leuA1 [H]

Identifier: 187929302

GI number: 187929302

Start: 2333409

End: 2334950

Strand: Reverse

Name: leuA1 [H]

Synonym: Rpic_2223

Alternate gene names: 187929302

Gene position: 2334950-2333409 (Counterclockwise)

Preceding gene: 187929303

Following gene: 187929300

Centisome position: 59.22

GC content: 62.78

Gene sequence:

>1542_bases
ATGAGCGACAAACTGATCATTTTCGATACCACCTTGCGCGACGGCGAGCAGTCCCCCGGGGCGTCGATGACCAAGGAAGA
GAAGATCCGCATCGCCAAGCAGCTGGAGCGGCTCAAGGTGGACGTGATCGAGGCCGGTTTTGCGGCCAGCTCCAATGGTG
ACTTCGAGGCGATCCGCGCGATTGCGCAGTCGGTCAAGGATTCGCGCATTTGCTCGCTGGCCCGTGCGAACGACCGTGAT
ATTTCCCGCGCTGCCGAGGCGCTCAAGCCGGCGGGGCAGTTCCGCATCCACACGTTCATCGCCACATCGGCGCTGCACAT
GGAAAAGAAGCTGCGCATGTCGCCGGATCAGGTGTACGAGCAGGCGCGCCTGGCGGTGCGTTTCGCGCGCCAGTTCACCG
ATGACATCGAGTTCTCGCCGGAAGATGGCAGCCGCTCCGATATGGATTTCCTCTGCCGCGTGCTGGAAGGCGTGATCGCC
GAGGGCGCCACCACCATCAACCTGCCGGATACGGTGGGCTACGCGGTGCCGGAAGGTTATGCCGCCCTGATCCGCTCGGT
GCGCGAGCGCATCCCGAATTCCGACAAGGCGGTCTGGTCGGTGCATTGCCATAACGACCTGGGCATGGCGGTCGCCAACT
CCCTGGCCGCTGTCAAACTGGGCGGCGCACGCCAGATTGAATGCACGATCAACGGCCTGGGCGAGCGCGCCGGCAACACC
AGCCTCGAAGAGGTGGTGATGGCCGTGAAGACGCGCCGCGACTACTTCAATCTCGACGTGGGTGTCGATACCACGCAGAT
CGTGCCGGCTTCCAAGCTGGTCTCGCAGATCACCGGTTTCGTTGTGCAGCCGAACAAGGCTGTCGTGGGCGCCAACGCTT
TCGCGCACGCTTCGGGCATCCACCAGGATGGCGTGCTCAAGGCGCGCGACACGTACGAAATCATGCGTGCCGAAGACGTG
GGCTGGACGGCCAACAAGATCGTCCTGGGCAAACTCTCGGGCCGCAACGCGTTCAAGCAGCGTCTGCAGGAACTGGGCAT
TGAACTGGATTCGGAAGCCGAACTGAACGCTGCCTTCACCCGCTTCAAGGAGCTGGCCGATCAGAAGGCTGAGATCTTCG
ACGAAGACATCGTCGCCATCGTCTCGAACGAAGCGCAGCACAGCGAGGGCGAGCACTTCCGTTTCGTGTCGCTGGCCCAG
CGTTCGGAAACCGGCGAGCGTCCGCACGCGCGCATCGTCTTCGTCGCGGATGGCAAGGAAGTGACGGGTGAAGCGGAGGG
CAATGGCCCGGTTGACGCCACGCTCAATGCGATTGAGAGCAAGGTCGCCAGCGGCGCCGAGCAACTGCTGTATTCGGTCA
ACGCCATCACGACGGGCACGCAGGCGCAGGGCGAAGTGACGGTGCGCCTGTCCAAGTCGGGCCGTATCGTCAACGGTGTG
GGTACTGACCCGGACATCGTTGCGGCTTCGGCCAAGGCTTACCTTGCCGCTCTGAACAAGCTGCAGGACAAGAGCAGCGA
AAAACTCAACCCGCAGATCTGA

Upstream 100 bases:

>100_bases
ACCATGCACGCCGGGGCGTTTTGCATTCTGCGCCGCAAAAAACCATTCGAGACACCCACTAAGACCGACGCCAAAAACGT
CACCCCACCAAGGAGCCGCC

Downstream 100 bases:

>100_bases
TCGCGGGTTTCATGAACACAGGCAGGAGTCAGCCCAGACCCCAACACGATCACCACTGAGCGCGCACCCGGCTATCCACC
GGAACGCGAAGCGGGTGCGG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1 [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MSDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERLKVDVIEAGFAASSNGDFEAIRAIAQSVKDSRICSLARANDRD
ISRAAEALKPAGQFRIHTFIATSALHMEKKLRMSPDQVYEQARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIA
EGATTINLPDTVGYAVPEGYAALIRSVRERIPNSDKAVWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNT
SLEEVVMAVKTRRDYFNLDVGVDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWTANKIVLGKLSGRNAFKQRLQELGIELDSEAELNAAFTRFKELADQKAEIFDEDIVAIVSNEAQHSEGEHFRFVSLAQ
RSETGERPHARIVFVADGKEVTGEAEGNGPVDATLNAIESKVASGAEQLLYSVNAITTGTQAQGEVTVRLSKSGRIVNGV
GTDPDIVAASAKAYLAALNKLQDKSSEKLNPQI

Sequences:

>Translated_513_residues
MSDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERLKVDVIEAGFAASSNGDFEAIRAIAQSVKDSRICSLARANDRD
ISRAAEALKPAGQFRIHTFIATSALHMEKKLRMSPDQVYEQARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIA
EGATTINLPDTVGYAVPEGYAALIRSVRERIPNSDKAVWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNT
SLEEVVMAVKTRRDYFNLDVGVDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWTANKIVLGKLSGRNAFKQRLQELGIELDSEAELNAAFTRFKELADQKAEIFDEDIVAIVSNEAQHSEGEHFRFVSLAQ
RSETGERPHARIVFVADGKEVTGEAEGNGPVDATLNAIESKVASGAEQLLYSVNAITTGTQAQGEVTVRLSKSGRIVNGV
GTDPDIVAASAKAYLAALNKLQDKSSEKLNPQI
>Mature_512_residues
SDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERLKVDVIEAGFAASSNGDFEAIRAIAQSVKDSRICSLARANDRDI
SRAAEALKPAGQFRIHTFIATSALHMEKKLRMSPDQVYEQARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIAE
GATTINLPDTVGYAVPEGYAALIRSVRERIPNSDKAVWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNTS
LEEVVMAVKTRRDYFNLDVGVDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVG
WTANKIVLGKLSGRNAFKQRLQELGIELDSEAELNAAFTRFKELADQKAEIFDEDIVAIVSNEAQHSEGEHFRFVSLAQR
SETGERPHARIVFVADGKEVTGEAEGNGPVDATLNAIESKVASGAEQLLYSVNAITTGTQAQGEVTVRLSKSGRIVNGVG
TDPDIVAASAKAYLAALNKLQDKSSEKLNPQI

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=502, Percent_Identity=49.601593625498, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6324682, Length=538, Percent_Identity=27.5092936802974, Blast_Score=147, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6324225, Length=538, Percent_Identity=27.3234200743494, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320019, Length=382, Percent_Identity=28.5340314136126, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6320071, Length=374, Percent_Identity=28.6096256684492, Blast_Score=129, Evalue=9e-31,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55545; Mature: 55414

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERLKVDVIEAGFAASSNGDFEAIRA
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAQSVKDSRICSLARANDRDISRAAEALKPAGQFRIHTFIATSALHMEKKLRMSPDQVYE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHH
QARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIAEGATTINLPDTVGYAVPEGY
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHH
AALIRSVRERIPNSDKAVWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNT
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCHHEEEECCCEEEEEEEECCCCCCCCC
SLEEVVMAVKTRRDYFNLDVGVDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCCEEEECCCCCHHCCCHHHHHHHHHCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGIELDSEAELNAAFT
CCCCCEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHH
RFKELADQKAEIFDEDIVAIVSNEAQHSEGEHFRFVSLAQRSETGERPHARIVFVADGKE
HHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEHHHHHCCCCCCCCEEEEEEECCCE
VTGEAEGNGPVDATLNAIESKVASGAEQLLYSVNAITTGTQAQGEVTVRLSKSGRIVNGV
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCCEEECC
GTDPDIVAASAKAYLAALNKLQDKSSEKLNPQI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERLKVDVIEAGFAASSNGDFEAIRA
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAQSVKDSRICSLARANDRDISRAAEALKPAGQFRIHTFIATSALHMEKKLRMSPDQVYE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHH
QARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIAEGATTINLPDTVGYAVPEGY
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHH
AALIRSVRERIPNSDKAVWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNT
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCHHEEEECCCEEEEEEEECCCCCCCCC
SLEEVVMAVKTRRDYFNLDVGVDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCCEEEECCCCCHHCCCHHHHHHHHHCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGIELDSEAELNAAFT
CCCCCEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHH
RFKELADQKAEIFDEDIVAIVSNEAQHSEGEHFRFVSLAQRSETGERPHARIVFVADGKE
HHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEHHHHHCCCCCCCCEEEEEEECCCE
VTGEAEGNGPVDATLNAIESKVASGAEQLLYSVNAITTGTQAQGEVTVRLSKSGRIVNGV
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCCEEECC
GTDPDIVAASAKAYLAALNKLQDKSSEKLNPQI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]