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The map label for this gene is prpE [H]

Identifier: 187929116

GI number: 187929116

Start: 2145873

End: 2147774

Strand: Reverse

Name: prpE [H]

Synonym: Rpic_2037

Alternate gene names: 187929116

Gene position: 2147774-2145873 (Counterclockwise)

Preceding gene: 187929117

Following gene: 187929115

Centisome position: 54.48

GC content: 66.3

Gene sequence:

>1902_bases
ATGCCCATGTCCGCCGCGTACCGCGCGCTGTATCAGCGCTCCATTGATGACCCCGCTGGCTTCTGGGGCGAACAGGCCCA
GCGCATCGAATGGCAGGGGCCGTATTCTGCCGTCCTGGACGATTCGCGCTTGCCATTCGCAAAGTGGTTCGTGGGCGGAC
GCACCAACCTGTGCCACAACGCCGTCGACCGGCATCTCGCCACGCGTGGCGAGCAGGCGGCGCTCGTGTATGTCTCCACT
GAAACCGGTCTGGAGGCCACGTACACGTATCGCCAGCTCCATCGCGAGGTCAACCGCATGGCGGCGAGCCTGCAGGCCCT
GGGCGTGAGGCGGGGCGACCGCGTGCTGATTTACCTGCCGATGATTCCTGAAGCCGCGTTTGCGATGCTGGCCTGTGCGC
GCATCGGCGCGATCCATTCGGTGGTGTTCGGCGGTTTCGCGTCCAACAGTCTCGCCACGCGCATCGACGACGCTACGCCG
CGCGTGATCGTCAGTGCCGATGCGGGCTCGCGAGCGGGCAAGGTGGTCGAGTACAAACCGTTGCTCGATGCCGCGATTGC
GCTGGCGGCCCACAAGCCCGAGCGCGTGCTGCTCGTCAATCGCCAACTCGCGCCCATGCAGCACAACCCGCGCGATGTCG
ACTACGCAACGCTCGCCGCCCAGCACGCGAATGCCGATGTGCCGTGCGAGTGGATGGAATCCAGCGAGCCCTCGTACATC
CTCTACACCTCCGGTACGACGGGCAAACCGAAAGGTGTCCAGCGCGACACCGGCGGCTATGCGGTGGCGCTCGCGGCGTC
GATGCCGCTCATTTTCGGTACGCAGCCCGGCGACACGATGTTCACCGCTTCGGACGTGGGCTGGGTGGTGGGACACAGCT
ACATCGTCTACGCGCCGCTGCTCGCGGGCCTGACGACGGTCATGTACGAAGGCACGCCAATCCGTCCCGACGGTGCGGTC
TGGTGGCGCATCGTCGAGCAATATCGGGTGAACGTGATGTTTACCGCACCGACCGCCATTCGCGTACTCAAAAAGCAGGA
TCCGGCGCTGCTGCAGCGACATGATCTGTCGAGCCTGCGCCGCCTCTTCCTTGCCGGCGAGCCGCTGGACGAGCCCACCG
CAAGCTGGATCGGCGAGGCACTGCACAAGCCCATCGTCGACAACTACTGGCAGACAGAGACCGGCTGGCCGATGCTGGCC
ATTCCGCAAGGGGTTGAGCCTTCCATGCAAAAGCTCGGCTCGCCGGGTTTCCCGGTCTATGGCTACAAGCTCGACATCCT
GGACGAGGCCACCGGCGAACCCTGCGCCCCCGGCGAGAAGGGGCTCCTCGCCGTTGCCGCGCCGCTGCCGCCGGGCTGCA
TGACGACCGTCTGGGGCGACGATGCCCGCTTTCTGCAGACATACTGGTCTGCGTTCCCGGGCCGGCACATCTATTCGAGC
TTCGACTGGGGTGTGCGCGACGAGGAGGGTTACGTGACCATCCTCGGCCGCACCGACGACGTCATCAACGTGGCGGGCCA
CCGTCTGGGCACCCGCGAGATCGAAGAAAGCCTGTCGTCGCATCCCGCCATCGCCGAGGTGGCGGTGGTGGGCGTGGCCG
ATCAGCTCAAAGGGCAGGTGGCGATGGGGTTTGCCATCGTGCGTGACGCTTCGCGCATTGCCGAGCCGACGGGGCGCCTG
GCCCTCGAAGGTGAGTTGATGCGCACAGTCGAGGTGCAGCTCGGGGCAGTGGCACGGCCCTCGCGCGTGTTCTTTGTCGG
GGCGCTGCCCAAGACGCGCTCCGGAAAGCTGCTGCGCCGGGCGATGCAGGCCGTAGCGGAAGGGCGTGACCCGGGAGATC
TGACGACCATTGAAGACCCCACGGCGCTGGCTCAGGTGCAGGCTGCCGTGCGAGGTGACTAG

Upstream 100 bases:

>100_bases
GGGGCGGCGGTAAGACCCGCGTAAGTTGGCGATCGCAAAGTAGGTGCATCAAGCACAACCAAGATTGAGCCATCGCGTAC
GATGGCCAGGAGACAACATC

Downstream 100 bases:

>100_bases
ATGATGAGAAAGTGACGTTCTCGCCAGCTCGATCACGGGCTCGTGGGCACGGCGGGACGTCACAAATTGGGCGGCACGCG
CCGTTAAGTCCTTCCTTTCA

Product: propionyl-CoA synthetase

Products: NA

Alternate protein names: Propionyl-CoA synthetase [H]

Number of amino acids: Translated: 633; Mature: 632

Protein sequence:

>633_residues
MPMSAAYRALYQRSIDDPAGFWGEQAQRIEWQGPYSAVLDDSRLPFAKWFVGGRTNLCHNAVDRHLATRGEQAALVYVST
ETGLEATYTYRQLHREVNRMAASLQALGVRRGDRVLIYLPMIPEAAFAMLACARIGAIHSVVFGGFASNSLATRIDDATP
RVIVSADAGSRAGKVVEYKPLLDAAIALAAHKPERVLLVNRQLAPMQHNPRDVDYATLAAQHANADVPCEWMESSEPSYI
LYTSGTTGKPKGVQRDTGGYAVALAASMPLIFGTQPGDTMFTASDVGWVVGHSYIVYAPLLAGLTTVMYEGTPIRPDGAV
WWRIVEQYRVNVMFTAPTAIRVLKKQDPALLQRHDLSSLRRLFLAGEPLDEPTASWIGEALHKPIVDNYWQTETGWPMLA
IPQGVEPSMQKLGSPGFPVYGYKLDILDEATGEPCAPGEKGLLAVAAPLPPGCMTTVWGDDARFLQTYWSAFPGRHIYSS
FDWGVRDEEGYVTILGRTDDVINVAGHRLGTREIEESLSSHPAIAEVAVVGVADQLKGQVAMGFAIVRDASRIAEPTGRL
ALEGELMRTVEVQLGAVARPSRVFFVGALPKTRSGKLLRRAMQAVAEGRDPGDLTTIEDPTALAQVQAAVRGD

Sequences:

>Translated_633_residues
MPMSAAYRALYQRSIDDPAGFWGEQAQRIEWQGPYSAVLDDSRLPFAKWFVGGRTNLCHNAVDRHLATRGEQAALVYVST
ETGLEATYTYRQLHREVNRMAASLQALGVRRGDRVLIYLPMIPEAAFAMLACARIGAIHSVVFGGFASNSLATRIDDATP
RVIVSADAGSRAGKVVEYKPLLDAAIALAAHKPERVLLVNRQLAPMQHNPRDVDYATLAAQHANADVPCEWMESSEPSYI
LYTSGTTGKPKGVQRDTGGYAVALAASMPLIFGTQPGDTMFTASDVGWVVGHSYIVYAPLLAGLTTVMYEGTPIRPDGAV
WWRIVEQYRVNVMFTAPTAIRVLKKQDPALLQRHDLSSLRRLFLAGEPLDEPTASWIGEALHKPIVDNYWQTETGWPMLA
IPQGVEPSMQKLGSPGFPVYGYKLDILDEATGEPCAPGEKGLLAVAAPLPPGCMTTVWGDDARFLQTYWSAFPGRHIYSS
FDWGVRDEEGYVTILGRTDDVINVAGHRLGTREIEESLSSHPAIAEVAVVGVADQLKGQVAMGFAIVRDASRIAEPTGRL
ALEGELMRTVEVQLGAVARPSRVFFVGALPKTRSGKLLRRAMQAVAEGRDPGDLTTIEDPTALAQVQAAVRGD
>Mature_632_residues
PMSAAYRALYQRSIDDPAGFWGEQAQRIEWQGPYSAVLDDSRLPFAKWFVGGRTNLCHNAVDRHLATRGEQAALVYVSTE
TGLEATYTYRQLHREVNRMAASLQALGVRRGDRVLIYLPMIPEAAFAMLACARIGAIHSVVFGGFASNSLATRIDDATPR
VIVSADAGSRAGKVVEYKPLLDAAIALAAHKPERVLLVNRQLAPMQHNPRDVDYATLAAQHANADVPCEWMESSEPSYIL
YTSGTTGKPKGVQRDTGGYAVALAASMPLIFGTQPGDTMFTASDVGWVVGHSYIVYAPLLAGLTTVMYEGTPIRPDGAVW
WRIVEQYRVNVMFTAPTAIRVLKKQDPALLQRHDLSSLRRLFLAGEPLDEPTASWIGEALHKPIVDNYWQTETGWPMLAI
PQGVEPSMQKLGSPGFPVYGYKLDILDEATGEPCAPGEKGLLAVAAPLPPGCMTTVWGDDARFLQTYWSAFPGRHIYSSF
DWGVRDEEGYVTILGRTDDVINVAGHRLGTREIEESLSSHPAIAEVAVVGVADQLKGQVAMGFAIVRDASRIAEPTGRLA
LEGELMRTVEVQLGAVARPSRVFFVGALPKTRSGKLLRRAMQAVAEGRDPGDLTTIEDPTALAQVQAAVRGD

Specific function: Catalyzes the synthesis of propionyl-CoA from propionate and CoA. Also converts acetate to acetyl-CoA but with a lower specific activity [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=635, Percent_Identity=43.1496062992126, Blast_Score=515, Evalue=1e-146,
Organism=Homo sapiens, GI28416953, Length=633, Percent_Identity=38.3886255924171, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI8923896, Length=664, Percent_Identity=36.7469879518072, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI253970489, Length=677, Percent_Identity=36.1890694239291, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI38505220, Length=616, Percent_Identity=26.1363636363636, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI31982927, Length=631, Percent_Identity=25.3565768621236, Blast_Score=170, Evalue=5e-42,
Organism=Homo sapiens, GI122937307, Length=540, Percent_Identity=29.2592592592593, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI42544132, Length=573, Percent_Identity=24.6073298429319, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI115511026, Length=558, Percent_Identity=26.3440860215054, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI58082049, Length=533, Percent_Identity=27.5797373358349, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI157311624, Length=544, Percent_Identity=27.5735294117647, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI157311622, Length=544, Percent_Identity=27.5735294117647, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI42544134, Length=362, Percent_Identity=22.6519337016575, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786529, Length=626, Percent_Identity=62.1405750798722, Blast_Score=799, Evalue=0.0,
Organism=Escherichia coli, GI1790505, Length=631, Percent_Identity=40.2535657686212, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI221142682, Length=543, Percent_Identity=23.7569060773481, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1786810, Length=529, Percent_Identity=24.007561436673, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI145693145, Length=549, Percent_Identity=23.1329690346084, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788107, Length=541, Percent_Identity=25.1386321626617, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71982997, Length=645, Percent_Identity=37.5193798449612, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71983001, Length=645, Percent_Identity=37.5193798449612, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17569507, Length=648, Percent_Identity=22.3765432098765, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71985884, Length=595, Percent_Identity=21.8487394957983, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560140, Length=542, Percent_Identity=22.8782287822878, Blast_Score=93, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17531443, Length=531, Percent_Identity=23.1638418079096, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319264, Length=645, Percent_Identity=39.3798449612403, Blast_Score=458, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6323182, Length=644, Percent_Identity=37.5776397515528, Blast_Score=422, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319699, Length=378, Percent_Identity=23.2804232804233, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355225, Length=652, Percent_Identity=40.1840490797546, Blast_Score=477, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24667955, Length=650, Percent_Identity=37.3846153846154, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI62472339, Length=559, Percent_Identity=38.6404293381038, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24667959, Length=529, Percent_Identity=37.6181474480151, Blast_Score=337, Evalue=2e-92,
Organism=Drosophila melanogaster, GI21355181, Length=537, Percent_Identity=24.2085661080074, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648260, Length=387, Percent_Identity=27.6485788113695, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648255, Length=472, Percent_Identity=22.6694915254237, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648253, Length=472, Percent_Identity=23.5169491525424, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI18859661, Length=530, Percent_Identity=22.4528301886792, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356441, Length=488, Percent_Identity=22.5409836065574, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20130357, Length=383, Percent_Identity=24.8041775456919, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR012694 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.17 [H]

Molecular weight: Translated: 68866; Mature: 68735

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00455 AMP_BINDING ; PS00201 FLAVODOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMSAAYRALYQRSIDDPAGFWGEQAQRIEWQGPYSAVLDDSRLPFAKWFVGGRTNLCHN
CCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHCCCCCCCHHHHEECCCCHHHHH
AVDRHLATRGEQAALVYVSTETGLEATYTYRQLHREVNRMAASLQALGVRRGDRVLIYLP
HHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
MIPEAAFAMLACARIGAIHSVVFGGFASNSLATRIDDATPRVIVSADAGSRAGKVVEYKP
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEEECCCCCCCCEEEEHH
LLDAAIALAAHKPERVLLVNRQLAPMQHNPRDVDYATLAAQHANADVPCEWMESSEPSYI
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHCCCCCCEE
LYTSGTTGKPKGVQRDTGGYAVALAASMPLIFGTQPGDTMFTASDVGWVVGHSYIVYAPL
EEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCCEEEECCCCHHHHCHHHHHHHH
LAGLTTVMYEGTPIRPDGAVWWRIVEQYRVNVMFTAPTAIRVLKKQDPALLQRHDLSSLR
HHHHHHHHCCCCCCCCCCHHHHHHHHHHEEEEEEECCHHHHHHHCCCHHHHHHHHHHHHH
RLFLAGEPLDEPTASWIGEALHKPIVDNYWQTETGWPMLAIPQGVEPSMQKLGSPGFPVY
HHHHCCCCCCCCHHHHHHHHHHCHHHCCCCCCCCCCEEEEECCCCCHHHHHCCCCCCCEE
GYKLDILDEATGEPCAPGEKGLLAVAAPLPPGCMTTVWGDDARFLQTYWSAFPGRHIYSS
EEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHCCCCHHCCC
FDWGVRDEEGYVTILGRTDDVINVAGHRLGTREIEESLSSHPAIAEVAVVGVADQLKGQV
CCCCCCCCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHCCCCHHHHEEEECHHHHCCCH
AMGFAIVRDASRIAEPTGRLALEGELMRTVEVQLGAVARPSRVFFVGALPKTRSGKLLRR
HHHHHHHHHHHHHCCCCCCEEECCHHHEEEEHHHCCCCCCCEEEEEECCCCCCCCHHHHH
AMQAVAEGRDPGDLTTIEDPTALAQVQAAVRGD
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PMSAAYRALYQRSIDDPAGFWGEQAQRIEWQGPYSAVLDDSRLPFAKWFVGGRTNLCHN
CCHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHCCCCCCCHHHHEECCCCHHHHH
AVDRHLATRGEQAALVYVSTETGLEATYTYRQLHREVNRMAASLQALGVRRGDRVLIYLP
HHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
MIPEAAFAMLACARIGAIHSVVFGGFASNSLATRIDDATPRVIVSADAGSRAGKVVEYKP
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEEECCCCCCCCEEEEHH
LLDAAIALAAHKPERVLLVNRQLAPMQHNPRDVDYATLAAQHANADVPCEWMESSEPSYI
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHCCCCCCEE
LYTSGTTGKPKGVQRDTGGYAVALAASMPLIFGTQPGDTMFTASDVGWVVGHSYIVYAPL
EEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCCEEEECCCCHHHHCHHHHHHHH
LAGLTTVMYEGTPIRPDGAVWWRIVEQYRVNVMFTAPTAIRVLKKQDPALLQRHDLSSLR
HHHHHHHHCCCCCCCCCCHHHHHHHHHHEEEEEEECCHHHHHHHCCCHHHHHHHHHHHHH
RLFLAGEPLDEPTASWIGEALHKPIVDNYWQTETGWPMLAIPQGVEPSMQKLGSPGFPVY
HHHHCCCCCCCCHHHHHHHHHHCHHHCCCCCCCCCCEEEEECCCCCHHHHHCCCCCCCEE
GYKLDILDEATGEPCAPGEKGLLAVAAPLPPGCMTTVWGDDARFLQTYWSAFPGRHIYSS
EEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHCCCCHHCCC
FDWGVRDEEGYVTILGRTDDVINVAGHRLGTREIEESLSSHPAIAEVAVVGVADQLKGQV
CCCCCCCCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHCCCCHHHHEEEECHHHHCCCH
AMGFAIVRDASRIAEPTGRLALEGELMRTVEVQLGAVARPSRVFFVGALPKTRSGKLLRR
HHHHHHHHHHHHHCCCCCCEEECCHHHEEEEHHHCCCCCCCEEEEEECCCCCCCCHHHHH
AMQAVAEGRDPGDLTTIEDPTALAQVQAAVRGD
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9006051; 10411265; 11677609; 10482501 [H]