Click here to switch to the map view.

The map label for this gene is carA [H]

Identifier: 187929115

GI number: 187929115

Start: 2144391

End: 2145527

Strand: Reverse

Name: carA [H]

Synonym: Rpic_2036

Alternate gene names: 187929115

Gene position: 2145527-2144391 (Counterclockwise)

Preceding gene: 187929116

Following gene: 187929114

Centisome position: 54.42

GC content: 63.32

Gene sequence:

>1137_bases
GTGTTGCCGTCTTTCCCGCCCGCCATTCTCGCGCTTGCAGACGGCACGGTCTTTCGTGGCTATTCCATCGGTGCGGCCGG
TCATACGATCGGCGAAGTGGTGTTCAACACCGCAATCACCGGCTATCAGGAAATCCTTACCGATCCGAGCTACTCGCGCC
AGATCGTCACGCTCACGTATCCGCATATCGGTAACGTTGGCGTGAACCGTGAGGATGTCGAAGCTACGAAAGTCCATGCC
GCCGGTCTCATCATCAAGGATCTCCCGATCCTGGCGTCGAACTTCCGCAAGGAGCACACGCTCTCGCATTACCTCAAGGG
CGAGAAAGTTGTCGCCATTGCCGGGATCGATACGCGCAAGCTGACCCGAATCCTGCGCGAGAAGGGCGCCCAGAACGGTT
GCATTCTCGCGGGCGAAGACAACGTGCAGAAGGCCATCGACCTCGCGCGCTCGTTCCCGGGCCTGTCGGGCATGGATCTG
GCGAAAGTCGTGTCCGTGACCCAGCCGTACGAGTGGACCCAGACCGAATGGGCGCTGGGCCGCGGCTACGGCAAGCAGGA
CGCCCCGAAGTATCACGTGGTCGCGTACGACTTTGGCGTCAAGTTCAACATCCTGCGCATGCTCGCCGAGCGTGGCTGCC
GCGTGACGGTGGTGCCGGCACAAACCAGCGCAGCCGACGTGCTGGCGCATAACCCGGATGGCGTCTTCCTTTCCAACGGC
CCCGGCGATCCGGAGCCGTGCGACTACGCTATCGCCGCCACCAGGGAGTTCCTGGAGCGTCGCCTGCCGACGTTCGGCAT
CTGTCTGGGCCACCAGATCATGGGCCTGGCTGTCGGTGCCAAGACGCTCAAGATGAAGACCGGCCACCACGGCGCGAATC
ACCCGGTCAAGGATCTGCAGGACGGCCGCGTGGTCATCACCTCGCAGAACCACGGTTTTGCAGTGGACCCGGAATCGCTG
CCGGCCAACGCGCGCGTGACGCACGTCTCGCTGTTCGACGGCACGCTGCAGGGCTTCGAGCTGACCGACCGTCCGGCGTT
CTGCTTCCAGGGCCACCCGGAAGCGTCGCCCGGTCCGCATGACATCGCGTATCTGTTTGACCGCTTCACCGCGGCGATGG
ACGCAGCCAAACAGTAA

Upstream 100 bases:

>100_bases
ACTCTCGGCGCGCAAACGGCCCGGATTCCGCATAGCCGGAATTCCGCTTCGGCGCCAAGGTGATCAGGTCGGCACAGGGT
GAATCCAGCAGGAGCTACCC

Downstream 100 bases:

>100_bases
AGCAACGAGAGCGCATCTGCCATGAACGCCTTCATGCTCGCCCACTTCGGCATCACCGAGTTCTGGACCTTTCTGCTCGG
CACGATTTTCATCGTGCTGC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MLPSFPPAILALADGTVFRGYSIGAAGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTYPHIGNVGVNREDVEATKVHA
AGLIIKDLPILASNFRKEHTLSHYLKGEKVVAIAGIDTRKLTRILREKGAQNGCILAGEDNVQKAIDLARSFPGLSGMDL
AKVVSVTQPYEWTQTEWALGRGYGKQDAPKYHVVAYDFGVKFNILRMLAERGCRVTVVPAQTSAADVLAHNPDGVFLSNG
PGDPEPCDYAIAATREFLERRLPTFGICLGHQIMGLAVGAKTLKMKTGHHGANHPVKDLQDGRVVITSQNHGFAVDPESL
PANARVTHVSLFDGTLQGFELTDRPAFCFQGHPEASPGPHDIAYLFDRFTAAMDAAKQ

Sequences:

>Translated_378_residues
MLPSFPPAILALADGTVFRGYSIGAAGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTYPHIGNVGVNREDVEATKVHA
AGLIIKDLPILASNFRKEHTLSHYLKGEKVVAIAGIDTRKLTRILREKGAQNGCILAGEDNVQKAIDLARSFPGLSGMDL
AKVVSVTQPYEWTQTEWALGRGYGKQDAPKYHVVAYDFGVKFNILRMLAERGCRVTVVPAQTSAADVLAHNPDGVFLSNG
PGDPEPCDYAIAATREFLERRLPTFGICLGHQIMGLAVGAKTLKMKTGHHGANHPVKDLQDGRVVITSQNHGFAVDPESL
PANARVTHVSLFDGTLQGFELTDRPAFCFQGHPEASPGPHDIAYLFDRFTAAMDAAKQ
>Mature_378_residues
MLPSFPPAILALADGTVFRGYSIGAAGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTYPHIGNVGVNREDVEATKVHA
AGLIIKDLPILASNFRKEHTLSHYLKGEKVVAIAGIDTRKLTRILREKGAQNGCILAGEDNVQKAIDLARSFPGLSGMDL
AKVVSVTQPYEWTQTEWALGRGYGKQDAPKYHVVAYDFGVKFNILRMLAERGCRVTVVPAQTSAADVLAHNPDGVFLSNG
PGDPEPCDYAIAATREFLERRLPTFGICLGHQIMGLAVGAKTLKMKTGHHGANHPVKDLQDGRVVITSQNHGFAVDPESL
PANARVTHVSLFDGTLQGFELTDRPAFCFQGHPEASPGPHDIAYLFDRFTAAMDAAKQ

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=382, Percent_Identity=38.7434554973822, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI169790915, Length=381, Percent_Identity=36.2204724409449, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI21361331, Length=381, Percent_Identity=36.2204724409449, Blast_Score=215, Evalue=5e-56,
Organism=Escherichia coli, GI1786215, Length=375, Percent_Identity=66.4, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1789760, Length=136, Percent_Identity=30.1470588235294, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=383, Percent_Identity=38.1201044386423, Blast_Score=244, Evalue=6e-65,
Organism=Saccharomyces cerevisiae, GI6324878, Length=378, Percent_Identity=40.4761904761905, Blast_Score=238, Evalue=9e-64,
Organism=Saccharomyces cerevisiae, GI6322331, Length=395, Percent_Identity=36.7088607594937, Blast_Score=236, Evalue=3e-63,
Organism=Drosophila melanogaster, GI45555749, Length=383, Percent_Identity=39.9477806788512, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24642586, Length=383, Percent_Identity=39.9477806788512, Blast_Score=233, Evalue=1e-61,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40895; Mature: 40895

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPSFPPAILALADGTVFRGYSIGAAGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTY
CCCCCCCCEEEEECCCEEECEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
PHIGNVGVNREDVEATKVHAAGLIIKDLPILASNFRKEHTLSHYLKGEKVVAIAGIDTRK
CCCCCCCCCCCCCHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHH
LTRILREKGAQNGCILAGEDNVQKAIDLARSFPGLSGMDLAKVVSVTQPYEWTQTEWALG
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHH
RGYGKQDAPKYHVVAYDFGVKFNILRMLAERGCRVTVVPAQTSAADVLAHNPDGVFLSNG
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCHHEEECCCEEEECCC
PGDPEPCDYAIAATREFLERRLPTFGICLGHQIMGLAVGAKTLKMKTGHHGANHPVKDLQ
CCCCCCCCHHHHHHHHHHHHHCCEEEHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHCC
DGRVVITSQNHGFAVDPESLPANARVTHVSLFDGTLQGFELTDRPAFCFQGHPEASPGPH
CCEEEEEECCCCEEECCCCCCCCCEEEEEEEECCCCCCCEECCCCCEEECCCCCCCCCCH
DIAYLFDRFTAAMDAAKQ
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLPSFPPAILALADGTVFRGYSIGAAGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTY
CCCCCCCCEEEEECCCEEECEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
PHIGNVGVNREDVEATKVHAAGLIIKDLPILASNFRKEHTLSHYLKGEKVVAIAGIDTRK
CCCCCCCCCCCCCHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHH
LTRILREKGAQNGCILAGEDNVQKAIDLARSFPGLSGMDLAKVVSVTQPYEWTQTEWALG
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHH
RGYGKQDAPKYHVVAYDFGVKFNILRMLAERGCRVTVVPAQTSAADVLAHNPDGVFLSNG
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCHHEEECCCEEEECCC
PGDPEPCDYAIAATREFLERRLPTFGICLGHQIMGLAVGAKTLKMKTGHHGANHPVKDLQ
CCCCCCCCHHHHHHHHHHHHHCCEEEHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHCC
DGRVVITSQNHGFAVDPESLPANARVTHVSLFDGTLQGFELTDRPAFCFQGHPEASPGPH
CCEEEEEECCCCEEECCCCCCCCCEEEEEEEECCCCCCCEECCCCCEEECCCCCCCCCCH
DIAYLFDRFTAAMDAAKQ
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]