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The map label for this gene is yeaS [C]

Identifier: 187929114

GI number: 187929114

Start: 2143695

End: 2144369

Strand: Reverse

Name: yeaS [C]

Synonym: Rpic_2035

Alternate gene names: 187929114

Gene position: 2144369-2143695 (Counterclockwise)

Preceding gene: 187929115

Following gene: 187929113

Centisome position: 54.39

GC content: 63.56

Gene sequence:

>675_bases
ATGAACGCCTTCATGCTCGCCCACTTCGGCATCACCGAGTTCTGGACCTTTCTGCTCGGCACGATTTTCATCGTGCTGCT
GCCGGGGCCGAATTCGATGTACGTGCTGTCGGTGGCGGCCCGCCGCGGTGTGCGCGCGGGCTACCAGGGCGCGTGCGGCG
TGTTTCTGGGTGATGCCATCCTGATGGTGTTGTCGGCAGCGGGCGTGGCTTCGCTGTTGAAGGCGAGTCCGGCGCTGTTC
TACGTCGTGAAGTACATCGGCGCGGCGTATCTGGCGTGGATCGGCCTGCAGATGCTGCGTGGCGCGGTTCGCAACTGGCG
TGTGCGCAAGGCCGGCGAGTCCGTCGCACAAGACGCGGCTCCGGCCGATGAATCGCACCCGTTCAAGAAGGCGCTGATCA
TCAGTCTGCTGAACCCGAAGGCGATCCTGTTCTTCATCTCGTTCTTCATCCAGTTCGTCGATCCGCACTACGCGCTGCCG
GCGGTGTCGTTCGTCGTGCTGGGGCTGGTCTGCCAGATCTGCAGCTTCCTGTACCTGACCACCATCATCTTCGTGGGCGC
GCGGCTGGCCGCGGCGTTCCGCGCGCGACGACGTTTGTCTGCCGGTATGAGCAGCGGCGTCGGCGCCATGTTTATCGGCT
TTTCCGCCAAGCTGGCGACGGCCACACTGAATTAA

Upstream 100 bases:

>100_bases
GGAAGCGTCGCCCGGTCCGCATGACATCGCGTATCTGTTTGACCGCTTCACCGCGGCGATGGACGCAGCCAAACAGTAAA
GCAACGAGAGCGCATCTGCC

Downstream 100 bases:

>100_bases
TCGAATCGAGCGGGTTATGCCAAAACGTACAGATATCAAAACCATCCTGATCATCGGCGCGGGTCCGATCATCATCGGCC
AGGCGTGTGAATTCGATTAC

Product: leucine export protein LeuE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MNAFMLAHFGITEFWTFLLGTIFIVLLPGPNSMYVLSVAARRGVRAGYQGACGVFLGDAILMVLSAAGVASLLKASPALF
YVVKYIGAAYLAWIGLQMLRGAVRNWRVRKAGESVAQDAAPADESHPFKKALIISLLNPKAILFFISFFIQFVDPHYALP
AVSFVVLGLVCQICSFLYLTTIIFVGARLAAAFRARRRLSAGMSSGVGAMFIGFSAKLATATLN

Sequences:

>Translated_224_residues
MNAFMLAHFGITEFWTFLLGTIFIVLLPGPNSMYVLSVAARRGVRAGYQGACGVFLGDAILMVLSAAGVASLLKASPALF
YVVKYIGAAYLAWIGLQMLRGAVRNWRVRKAGESVAQDAAPADESHPFKKALIISLLNPKAILFFISFFIQFVDPHYALP
AVSFVVLGLVCQICSFLYLTTIIFVGARLAAAFRARRRLSAGMSSGVGAMFIGFSAKLATATLN
>Mature_224_residues
MNAFMLAHFGITEFWTFLLGTIFIVLLPGPNSMYVLSVAARRGVRAGYQGACGVFLGDAILMVLSAAGVASLLKASPALF
YVVKYIGAAYLAWIGLQMLRGAVRNWRVRKAGESVAQDAAPADESHPFKKALIISLLNPKAILFFISFFIQFVDPHYALP
AVSFVVLGLVCQICSFLYLTTIIFVGARLAAAFRARRRLSAGMSSGVGAMFIGFSAKLATATLN

Specific function: Unknown

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI1788099, Length=216, Percent_Identity=43.9814814814815, Blast_Score=188, Evalue=2e-49,
Organism=Escherichia coli, GI48994979, Length=210, Percent_Identity=28.0952380952381, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI48994978, Length=191, Percent_Identity=26.7015706806283, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1786522, Length=122, Percent_Identity=33.6065573770492, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 24097; Mature: 24097

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAFMLAHFGITEFWTFLLGTIFIVLLPGPNSMYVLSVAARRGVRAGYQGACGVFLGDAI
CCCEEEHHCCHHHHHHHHHHHHHHHEECCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHH
LMVLSAAGVASLLKASPALFYVVKYIGAAYLAWIGLQMLRGAVRNWRVRKAGESVAQDAA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
PADESHPFKKALIISLLNPKAILFFISFFIQFVDPHYALPAVSFVVLGLVCQICSFLYLT
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
TIIFVGARLAAAFRARRRLSAGMSSGVGAMFIGFSAKLATATLN
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNAFMLAHFGITEFWTFLLGTIFIVLLPGPNSMYVLSVAARRGVRAGYQGACGVFLGDAI
CCCEEEHHCCHHHHHHHHHHHHHHHEECCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHH
LMVLSAAGVASLLKASPALFYVVKYIGAAYLAWIGLQMLRGAVRNWRVRKAGESVAQDAA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
PADESHPFKKALIISLLNPKAILFFISFFIQFVDPHYALPAVSFVVLGLVCQICSFLYLT
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
TIIFVGARLAAAFRARRRLSAGMSSGVGAMFIGFSAKLATATLN
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8169201; 10984043 [H]