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The map label for this gene is fadH [C]

Identifier: 187929087

GI number: 187929087

Start: 2114820

End: 2116055

Strand: Reverse

Name: fadH [C]

Synonym: Rpic_2008

Alternate gene names: 187929087

Gene position: 2116055-2114820 (Counterclockwise)

Preceding gene: 187929090

Following gene: 187929086

Centisome position: 53.67

GC content: 67.56

Gene sequence:

>1236_bases
ATGAAGCTCTTCACGCCCCTCGTTCTGCCCAACGGCGCGGTGATCCCAAATCGCCTTGCCAAAGCCGCCATGGAAGAAAA
CATGGCCGATGCCGATCACGCGCCTTCCGACGCGTTGCTGCGCCTGTATGAGGCGTGGGCGGCAGGTGGCGCGGGCTTGA
TCCTGACCGGCAACGTGATGATCGACCACCGCGCCATGACCGGCCCGAACGGCGTCGTCCTCGAAGACGGCGCGCATCTC
GAGCGCTTCCGCCGCTGGGCCCAGGTGGCGCGTGCGCACGGCGCGCATGCGTGGGTGCAGATCAACCATCCGGGGCGCCA
GATGCCGGCATCGCTCGGGCAGCCGACGCTGGCGCCGTCGGCAGTTCCGCTGGCGTTGGGCGCGCTGTCCAAGCAGTTTC
CGGTGCCGCGCGAGATGACCGAGGTGGATATCGGCGAAGTCCAGCAGCGCTTCACGCGCAGCGCGCAACTGGCCGAGCAG
AGCGGCTTTACCGGCGTGCAGATCCACGCCGCCCATGGCTATCTGCTGAGCCAGTTTCTGTCGCCCATTTCCAACCGGCG
GCAGGATCAGTGGGGCGGCCCGATCGAAAACCGTGCGCGCCTGCTGCTCGATATCGTGCGCAGCGTGCGTGCCGCCGTGT
CGCCGCAGTTCGTGGTGGCGGTCAAGCTGAACTCGGCCGATTTCCAGCGCGGCGGCTTCAGCCCTGACGATGCGAAGCAG
GTGGTCGAGCTGCTCAACCCGCTGGGCGTCGATCTGGTCGAACTGTCCGGCGGCAGCTATGAAGTGCCTGCCATGCAAGG
GCACGCGCGCGACGGCCGCACGCTTGCCCGCGAAGCGTATTTCCTGGAATTTGCGCGCGACATTGCCGCCATCGCACGCA
TGCCGCTGATGGTCACGGGCGGCATCCGGCGTCGGGCGGTGGCGGAGCAGGTGGTCGACAGCGGTGTCGATATGGTGGGC
ATTGCGACTGCGCTGTCCATCGAACCAAACCTGCCGCGCGACTGGGCTGGCGGCAAGAACAACGCGCCCGCGCTGCAGAC
CATCACGTGGAAGAACAAGGCGCTGGGCGCACTGGCCAACATGGCGGTCGTCAAGTTTCAGCTCAACCGGCTGAGCGAGG
GGCGCACGACGGACCCGCGCGTGTCGCCGCTGCGCGCGCTGATTCAGCAGCAACTGGCCGCAGCGTGCCAGTCGCGGCGC
TATCGTCGATGGACGGCGCAACGCCAGGCGCGCTAG

Upstream 100 bases:

>100_bases
ACCAATCTTCATGTGCGTGCTCCGCAGACTTGACATTCAAGTTGACTTTAAGCTTAGGGTTGAGGTCTCGTCAAATGTCA
AATCCCACGCGGATGCCACC

Downstream 100 bases:

>100_bases
CGCGTATTTTCTGACCGGACCGGCGCAGCCTTACCGATGCGCCGCGTCTCTTGTAGATTCATGTTTTGCCTCCGATCGGA
AAGCACGACATGAAACACGT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MKLFTPLVLPNGAVIPNRLAKAAMEENMADADHAPSDALLRLYEAWAAGGAGLILTGNVMIDHRAMTGPNGVVLEDGAHL
ERFRRWAQVARAHGAHAWVQINHPGRQMPASLGQPTLAPSAVPLALGALSKQFPVPREMTEVDIGEVQQRFTRSAQLAEQ
SGFTGVQIHAAHGYLLSQFLSPISNRRQDQWGGPIENRARLLLDIVRSVRAAVSPQFVVAVKLNSADFQRGGFSPDDAKQ
VVELLNPLGVDLVELSGGSYEVPAMQGHARDGRTLAREAYFLEFARDIAAIARMPLMVTGGIRRRAVAEQVVDSGVDMVG
IATALSIEPNLPRDWAGGKNNAPALQTITWKNKALGALANMAVVKFQLNRLSEGRTTDPRVSPLRALIQQQLAAACQSRR
YRRWTAQRQAR

Sequences:

>Translated_411_residues
MKLFTPLVLPNGAVIPNRLAKAAMEENMADADHAPSDALLRLYEAWAAGGAGLILTGNVMIDHRAMTGPNGVVLEDGAHL
ERFRRWAQVARAHGAHAWVQINHPGRQMPASLGQPTLAPSAVPLALGALSKQFPVPREMTEVDIGEVQQRFTRSAQLAEQ
SGFTGVQIHAAHGYLLSQFLSPISNRRQDQWGGPIENRARLLLDIVRSVRAAVSPQFVVAVKLNSADFQRGGFSPDDAKQ
VVELLNPLGVDLVELSGGSYEVPAMQGHARDGRTLAREAYFLEFARDIAAIARMPLMVTGGIRRRAVAEQVVDSGVDMVG
IATALSIEPNLPRDWAGGKNNAPALQTITWKNKALGALANMAVVKFQLNRLSEGRTTDPRVSPLRALIQQQLAAACQSRR
YRRWTAQRQAR
>Mature_411_residues
MKLFTPLVLPNGAVIPNRLAKAAMEENMADADHAPSDALLRLYEAWAAGGAGLILTGNVMIDHRAMTGPNGVVLEDGAHL
ERFRRWAQVARAHGAHAWVQINHPGRQMPASLGQPTLAPSAVPLALGALSKQFPVPREMTEVDIGEVQQRFTRSAQLAEQ
SGFTGVQIHAAHGYLLSQFLSPISNRRQDQWGGPIENRARLLLDIVRSVRAAVSPQFVVAVKLNSADFQRGGFSPDDAKQ
VVELLNPLGVDLVELSGGSYEVPAMQGHARDGRTLAREAYFLEFARDIAAIARMPLMVTGGIRRRAVAEQVVDSGVDMVG
IATALSIEPNLPRDWAGGKNNAPALQTITWKNKALGALANMAVVKFQLNRLSEGRTTDPRVSPLRALIQQQLAAACQSRR
YRRWTAQRQAR

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=385, Percent_Identity=25.974025974026, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787939, Length=223, Percent_Identity=33.6322869955157, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=344, Percent_Identity=38.0813953488372, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17565138, Length=343, Percent_Identity=36.1516034985423, Blast_Score=217, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI72001454, Length=346, Percent_Identity=35.8381502890173, Blast_Score=211, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI17564188, Length=349, Percent_Identity=35.243553008596, Blast_Score=208, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17540738, Length=348, Percent_Identity=34.1954022988506, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17559804, Length=349, Percent_Identity=33.5243553008596, Blast_Score=201, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI17566914, Length=356, Percent_Identity=36.2359550561798, Blast_Score=184, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI17566916, Length=348, Percent_Identity=31.0344827586207, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321973, Length=149, Percent_Identity=31.5436241610738, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6325086, Length=80, Percent_Identity=42.5, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 44702; Mature: 44702

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: PS01034 GLYCOSYL_HYDROL_F16

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFTPLVLPNGAVIPNRLAKAAMEENMADADHAPSDALLRLYEAWAAGGAGLILTGNVM
CCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCEE
IDHRAMTGPNGVVLEDGAHLERFRRWAQVARAHGAHAWVQINHPGRQMPASLGQPTLAPS
EECEECCCCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHCCCCCCCCC
AVPLALGALSKQFPVPREMTEVDIGEVQQRFTRSAQLAEQSGFTGVQIHAAHGYLLSQFL
HHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHH
SPISNRRQDQWGGPIENRARLLLDIVRSVRAAVSPQFVVAVKLNSADFQRGGFSPDDAKQ
HHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHH
VVELLNPLGVDLVELSGGSYEVPAMQGHARDGRTLAREAYFLEFARDIAAIARMPLMVTG
HHHHHCCCCCEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEEC
GIRRRAVAEQVVDSGVDMVGIATALSIEPNLPRDWAGGKNNAPALQTITWKNKALGALAN
CHHHHHHHHHHHHCCCCEEEHHEEEEECCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHH
MAVVKFQLNRLSEGRTTDPRVSPLRALIQQQLAAACQSRRYRRWTAQRQAR
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLFTPLVLPNGAVIPNRLAKAAMEENMADADHAPSDALLRLYEAWAAGGAGLILTGNVM
CCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCEE
IDHRAMTGPNGVVLEDGAHLERFRRWAQVARAHGAHAWVQINHPGRQMPASLGQPTLAPS
EECEECCCCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHCCCCCCCCC
AVPLALGALSKQFPVPREMTEVDIGEVQQRFTRSAQLAEQSGFTGVQIHAAHGYLLSQFL
HHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHH
SPISNRRQDQWGGPIENRARLLLDIVRSVRAAVSPQFVVAVKLNSADFQRGGFSPDDAKQ
HHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHH
VVELLNPLGVDLVELSGGSYEVPAMQGHARDGRTLAREAYFLEFARDIAAIARMPLMVTG
HHHHHCCCCCEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEEC
GIRRRAVAEQVVDSGVDMVGIATALSIEPNLPRDWAGGKNNAPALQTITWKNKALGALAN
CHHHHHHHHHHHHCCCCEEEHHEEEEECCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHH
MAVVKFQLNRLSEGRTTDPRVSPLRALIQQQLAAACQSRRYRRWTAQRQAR
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]