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The map label for this gene is hpcG [H]

Identifier: 187929035

GI number: 187929035

Start: 2065896

End: 2066699

Strand: Reverse

Name: hpcG [H]

Synonym: Rpic_1955

Alternate gene names: 187929035

Gene position: 2066699-2065896 (Counterclockwise)

Preceding gene: 187929036

Following gene: 187929034

Centisome position: 52.42

GC content: 64.43

Gene sequence:

>804_bases
ATGCTCGACACCACGACCATCCAAACCATCGCGCGCCGCTTGCACCAGGCCGAGATCGATCGCAAGCAGATCCGCCAGGT
TTCGCTTGACCACCCCGAGATCACCATCGAAGACGCCTACGCCATCCAGCGCGAATGGGTCGCGATGAAGCAGGCAGAGG
GGCGACGCCTGAAGGGGCACAAGATCGGACTGACCTCACGTGCGATGCAGCAGTCGTCACAGATCGACGAGCCGGACTAC
GGTGCGCTGCTCGACGACATGTTCTTTGCCGAGCGCAGTGAGATCCCCGCCAGCCGTTTCATCGTGCCGCGTGTGGAAGT
GGAACTCGCCTTCGTGCTCGGTAAGCAGCTGGAGGGGCCGGAGGTGACGCTGTTCGACGTGTATGACGCGGTGGATCACG
TGATTCCCGCGCTGGAGATCATCGACGCGCGCTCGCAATCGATCGATCCCGACAGCGGGCGTCCACGCAAGGTGTTTGAC
ACGATCGCCGACAACGCCGCCAATGCCGGCGTCGTGATGGGCGGCCGGCCCATCCGCATGCGCGATGTGGATCTGCGCTG
GGTCAGCGCCATCTTTTCACGCAACGCCGTCATCGAAGAGACGGGTGTGGCGGCCGGCGTGCTGAATCACCCGGCCAACG
GTGTCGTCTGGCTGGCCAACAAGCTGGCGCCGCACGGCGTGGCGCTGGAGCCCGGGCAGGTCATCCTTGGCGGCTCGTTC
ACGCGGCCGGTGGCCGCTCGGCCCGGTGATACATTCCATTGCGACTACGGTCCGCTTGGCAGCATCGCCTGTCATTTCGC
CTAA

Upstream 100 bases:

>100_bases
GCGCCGTTATCTGGCGCTGTCGATGGAGTTGACCGAGTTTGACGAGGGCGGCAGCTACAAGCACAACAACATCCACGCGC
GTTTTCGCAAGGCTTGACGC

Downstream 100 bases:

>100_bases
CCGTCTAACGCTCAAAGGATGGCCTCGCCATGCAACTTCCGGTGAACAACTTCAAGCGCGCGCTGGCCGAACGCCGGCCG
CAGATCGGCCTGTGGCTGGC

Product: 2-oxo-hepta-3-ene-1,7-dioic acid hydratase

Products: H2O; 2-oxopent-4-enoate [C]

Alternate protein names: OHED hydratase [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MLDTTTIQTIARRLHQAEIDRKQIRQVSLDHPEITIEDAYAIQREWVAMKQAEGRRLKGHKIGLTSRAMQQSSQIDEPDY
GALLDDMFFAERSEIPASRFIVPRVEVELAFVLGKQLEGPEVTLFDVYDAVDHVIPALEIIDARSQSIDPDSGRPRKVFD
TIADNAANAGVVMGGRPIRMRDVDLRWVSAIFSRNAVIEETGVAAGVLNHPANGVVWLANKLAPHGVALEPGQVILGGSF
TRPVAARPGDTFHCDYGPLGSIACHFA

Sequences:

>Translated_267_residues
MLDTTTIQTIARRLHQAEIDRKQIRQVSLDHPEITIEDAYAIQREWVAMKQAEGRRLKGHKIGLTSRAMQQSSQIDEPDY
GALLDDMFFAERSEIPASRFIVPRVEVELAFVLGKQLEGPEVTLFDVYDAVDHVIPALEIIDARSQSIDPDSGRPRKVFD
TIADNAANAGVVMGGRPIRMRDVDLRWVSAIFSRNAVIEETGVAAGVLNHPANGVVWLANKLAPHGVALEPGQVILGGSF
TRPVAARPGDTFHCDYGPLGSIACHFA
>Mature_267_residues
MLDTTTIQTIARRLHQAEIDRKQIRQVSLDHPEITIEDAYAIQREWVAMKQAEGRRLKGHKIGLTSRAMQQSSQIDEPDY
GALLDDMFFAERSEIPASRFIVPRVEVELAFVLGKQLEGPEVTLFDVYDAVDHVIPALEIIDARSQSIDPDSGRPRKVFD
TIADNAANAGVVMGGRPIRMRDVDLRWVSAIFSRNAVIEETGVAAGVLNHPANGVVWLANKLAPHGVALEPGQVILGGSF
TRPVAARPGDTFHCDYGPLGSIACHFA

Specific function: Transforms 2-oxo-hept-3-ene-1,7-dioate (OHED) into 2,4- dihydoxy-hept-2-ene-1,7-dioic acid (HHED) [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=231, Percent_Identity=37.2294372294372, Blast_Score=151, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012690 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 29340; Mature: 29340

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDTTTIQTIARRLHQAEIDRKQIRQVSLDHPEITIEDAYAIQREWVAMKQAEGRRLKGH
CCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCC
KIGLTSRAMQQSSQIDEPDYGALLDDMFFAERSEIPASRFIVPRVEVELAFVLGKQLEGP
EECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHEEECHHHEEEEEECCCCCCCC
EVTLFDVYDAVDHVIPALEIIDARSQSIDPDSGRPRKVFDTIADNAANAGVVMGGRPIRM
CEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEECCCCEEE
RDVDLRWVSAIFSRNAVIEETGVAAGVLNHPANGVVWLANKLAPHGVALEPGQVILGGSF
ECCHHHHHHHHHHCCCEEECCCCCHHHHCCCCCCEEEEECCCCCCCEEECCCCEEECCCC
TRPVAARPGDTFHCDYGPLGSIACHFA
CCCCCCCCCCEEEECCCCCCCEEEECC
>Mature Secondary Structure
MLDTTTIQTIARRLHQAEIDRKQIRQVSLDHPEITIEDAYAIQREWVAMKQAEGRRLKGH
CCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCC
KIGLTSRAMQQSSQIDEPDYGALLDDMFFAERSEIPASRFIVPRVEVELAFVLGKQLEGP
EECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHEEECHHHEEEEEECCCCCCCC
EVTLFDVYDAVDHVIPALEIIDARSQSIDPDSGRPRKVFDTIADNAANAGVVMGGRPIRM
CEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEECCCCEEE
RDVDLRWVSAIFSRNAVIEETGVAAGVLNHPANGVVWLANKLAPHGVALEPGQVILGGSF
ECCHHHHHHHHHHCCCEEECCCCCHHHHCCCCCCEEEEECCCCCCCEEECCCCEEECCCC
TRPVAARPGDTFHCDYGPLGSIACHFA
CCCCCCCCCCEEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxy-2-ketovalerate [C]

Specific reaction: 4-hydroxy-2-ketovalerate = H2O + 2-oxopent-4-enoate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7737515 [H]