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The map label for this gene is hpcH [H]

Identifier: 187929034

GI number: 187929034

Start: 2065060

End: 2065866

Strand: Reverse

Name: hpcH [H]

Synonym: Rpic_1954

Alternate gene names: 187929034

Gene position: 2065866-2065060 (Counterclockwise)

Preceding gene: 187929035

Following gene: 187929027

Centisome position: 52.4

GC content: 66.17

Gene sequence:

>807_bases
ATGCAACTTCCGGTGAACAACTTCAAGCGCGCGCTGGCCGAACGCCGGCCGCAGATCGGCCTGTGGCTGGCAATGACGCA
TCCGTACCCGGCGGAGATCGTCGCGGGCGCCGGCTTTGACTGGCTGCTGATCGACAACGAGCACGCGCCAAACCAACTGC
AATCGACGCTCGCGCAATTGCAGGCGGTTGCGCCGTATGCTTCGCATCCCATCGTGCGGCCTGCCTGGAACGATTCGGTC
GAGATCAAGCGCCTGCTGGATATCGGCGCGCAGACCCTGCTGGTGCCGATGGTCCAGAACGCCGACGAAGCGCGTGCCGC
CGTGGAAGCGATGCGCTATCCGCCCAACGGTGTTCGCGGCGTTGGCAGCGCGTTGGCTCGCGCATCGCGCTGGAACCGCG
TGGGCGATTACCTCCACCAGGCCGATGCGCAGATGTGCACGCTCGTCCAGGTCGAAACTGCAGAGAGCCTGGAGCATCTC
GACGCGATTCTTGCTGTGCAAGGCGTGGACGGCGTCTTCATCGGCCCGGGCGATCTCGCGGCGTCCATGGGGCACCTCGG
CGCGCCGGGGCATCCGGAAGTCAGCCAGGCGATCGACGGCGCGATTCAACGCATCGTCGCGTCGGGCAAGGCGGCGGGCA
TTCTGTCGGCGAATCAGGCGCAAGCGAAGCACTACCTGTCGCTCGGTGCGACCTTTGTTGCGGTAGGGACGGATGTCACC
CTGCTGGCGCGCAGCGCCGAACAGCTGGCGCAGGCATTCAAGGAAGAGGGCGGTGCCAAGCCGCAGCCGGCCGGCAGCGT
GTATTGA

Upstream 100 bases:

>100_bases
CCGCTCGGCCCGGTGATACATTCCATTGCGACTACGGTCCGCTTGGCAGCATCGCCTGTCATTTCGCCTAACCGTCTAAC
GCTCAAAGGATGGCCTCGCC

Downstream 100 bases:

>100_bases
CGGGTGTTACGCGTCGCCAGTGAAGCGCCCCATCGGCGTGCGCGTTTCCATGACGTGGATCGACTCATCGATGGCGGCGT
AGAGCGCGTCCAGCCGCGCG

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MQLPVNNFKRALAERRPQIGLWLAMTHPYPAEIVAGAGFDWLLIDNEHAPNQLQSTLAQLQAVAPYASHPIVRPAWNDSV
EIKRLLDIGAQTLLVPMVQNADEARAAVEAMRYPPNGVRGVGSALARASRWNRVGDYLHQADAQMCTLVQVETAESLEHL
DAILAVQGVDGVFIGPGDLAASMGHLGAPGHPEVSQAIDGAIQRIVASGKAAGILSANQAQAKHYLSLGATFVAVGTDVT
LLARSAEQLAQAFKEEGGAKPQPAGSVY

Sequences:

>Translated_268_residues
MQLPVNNFKRALAERRPQIGLWLAMTHPYPAEIVAGAGFDWLLIDNEHAPNQLQSTLAQLQAVAPYASHPIVRPAWNDSV
EIKRLLDIGAQTLLVPMVQNADEARAAVEAMRYPPNGVRGVGSALARASRWNRVGDYLHQADAQMCTLVQVETAESLEHL
DAILAVQGVDGVFIGPGDLAASMGHLGAPGHPEVSQAIDGAIQRIVASGKAAGILSANQAQAKHYLSLGATFVAVGTDVT
LLARSAEQLAQAFKEEGGAKPQPAGSVY
>Mature_268_residues
MQLPVNNFKRALAERRPQIGLWLAMTHPYPAEIVAGAGFDWLLIDNEHAPNQLQSTLAQLQAVAPYASHPIVRPAWNDSV
EIKRLLDIGAQTLLVPMVQNADEARAAVEAMRYPPNGVRGVGSALARASRWNRVGDYLHQADAQMCTLVQVETAESLEHL
DAILAVQGVDGVFIGPGDLAASMGHLGAPGHPEVSQAIDGAIQRIVASGKAAGILSANQAQAKHYLSLGATFVAVGTDVT
LLARSAEQLAQAFKEEGGAKPQPAGSVY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=261, Percent_Identity=52.4904214559387, Blast_Score=270, Evalue=9e-74,
Organism=Escherichia coli, GI1789514, Length=250, Percent_Identity=46.8, Blast_Score=239, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 28386; Mature: 28386

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLPVNNFKRALAERRPQIGLWLAMTHPYPAEIVAGAGFDWLLIDNEHAPNQLQSTLAQL
CCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHEECCCCEEEEEECCCCHHHHHHHHHHH
QAVAPYASHPIVRPAWNDSVEIKRLLDIGAQTLLVPMVQNADEARAAVEAMRYPPNGVRG
HHHCCHHCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHH
VGSALARASRWNRVGDYLHQADAQMCTLVQVETAESLEHLDAILAVQGVDGVFIGPGDLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCEEEECCHHHH
ASMGHLGAPGHPEVSQAIDGAIQRIVASGKAAGILSANQAQAKHYLSLGATFVAVGTDVT
HHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHCCEEEEECCCHH
LLARSAEQLAQAFKEEGGAKPQPAGSVY
HHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MQLPVNNFKRALAERRPQIGLWLAMTHPYPAEIVAGAGFDWLLIDNEHAPNQLQSTLAQL
CCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHEECCCCEEEEEECCCCHHHHHHHHHHH
QAVAPYASHPIVRPAWNDSVEIKRLLDIGAQTLLVPMVQNADEARAAVEAMRYPPNGVRG
HHHCCHHCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHH
VGSALARASRWNRVGDYLHQADAQMCTLVQVETAESLEHLDAILAVQGVDGVFIGPGDLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCEEEECCHHHH
ASMGHLGAPGHPEVSQAIDGAIQRIVASGKAAGILSANQAQAKHYLSLGATFVAVGTDVT
HHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHCCEEEEECCCHH
LLARSAEQLAQAFKEEGGAKPQPAGSVY
HHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA