Click here to switch to the map view.

The map label for this gene is fixJ [H]

Identifier: 187929029

GI number: 187929029

Start: 2059807

End: 2060442

Strand: Direct

Name: fixJ [H]

Synonym: Rpic_1949

Alternate gene names: 187929029

Gene position: 2059807-2060442 (Clockwise)

Preceding gene: 187929028

Following gene: 187929030

Centisome position: 52.25

GC content: 63.05

Gene sequence:

>636_bases
ATGAGCACAACGCCCGCCGCAGTTGCGCCGCGCAACGAAACCGTGTTCGTCGTCGATGACGATGAAGCCATGCGTGACTC
GTTGACGTGGCTGCTCGAAGGCAACGGCTACACCGTCCGCACCTACCGCAGCGCAGAAGAATTCCTGGTCGACGACAAGC
GCGGCGAAGGCGTCGGCTGCCTGATCCTTGACGTGCGCATGCAAGGCATGAGCGGCCCCGAGCTGCAAGACCGCCTGTTG
GCAGAGAACAACCGCATGCCGATCGTCTTCGTCACGGGCCACGGCGACGTGCCGTTGGCCGTGTCGACCATGAAGAAAGG
CGCGGTCGACTTCATTGAAAAGCCGTTCGACGAATCCGAACTGCGCGAGCTGGTGGAACGCATGCTCAGCAAGGCGCGCA
GCGAAGACTCGGTCGCCCGCGAGCAAAAGGCCGCCAAGGACCTGCTCGGCCGCCTGACCACACGCGAGCAGCAAGTGCTG
GAGCGCATCGTCGCCGGCCGCCTGAACAAGCAGATCGCCGACGATCTGGGCATTTCCATCAAGACGGTGGAAGCACACCG
CGCCAACATCATGGAAAAGCTCAACGTGAATACGGTGGCAGACCTGCTGCGCCTGGCCCTGTCGCGCAACGCCTGA

Upstream 100 bases:

>100_bases
AACAACGCCGATGGCATCGGCTGTACGTTTAAAATCATCTTGCCGCTGAATCCGCTGTAAATCGTTTTTAAAACATAGAA
CGTAGACCGAGGACTCCTCC

Downstream 100 bases:

>100_bases
TTCGGCTGCAGGAAAAACCAAGCGATCATCCGGGCGGCCGGAAGGGAGGCGGCGATTTATAATCGCGGTTTGCCCTCTCC
CTCCTCCGCCCAGCCATGAC

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MSTTPAAVAPRNETVFVVDDDEAMRDSLTWLLEGNGYTVRTYRSAEEFLVDDKRGEGVGCLILDVRMQGMSGPELQDRLL
AENNRMPIVFVTGHGDVPLAVSTMKKGAVDFIEKPFDESELRELVERMLSKARSEDSVAREQKAAKDLLGRLTTREQQVL
ERIVAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSRNA

Sequences:

>Translated_211_residues
MSTTPAAVAPRNETVFVVDDDEAMRDSLTWLLEGNGYTVRTYRSAEEFLVDDKRGEGVGCLILDVRMQGMSGPELQDRLL
AENNRMPIVFVTGHGDVPLAVSTMKKGAVDFIEKPFDESELRELVERMLSKARSEDSVAREQKAAKDLLGRLTTREQQVL
ERIVAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSRNA
>Mature_210_residues
STTPAAVAPRNETVFVVDDDEAMRDSLTWLLEGNGYTVRTYRSAEEFLVDDKRGEGVGCLILDVRMQGMSGPELQDRLLA
ENNRMPIVFVTGHGDVPLAVSTMKKGAVDFIEKPFDESELRELVERMLSKARSEDSVAREQKAAKDLLGRLTTREQQVLE
RIVAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSRNA

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=121, Percent_Identity=35.5371900826446, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790102, Length=195, Percent_Identity=32.3076923076923, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786747, Length=204, Percent_Identity=26.4705882352941, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788222, Length=202, Percent_Identity=26.7326732673267, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1790437, Length=126, Percent_Identity=32.5396825396825, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI1789809, Length=149, Percent_Identity=32.2147651006711, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1790860, Length=123, Percent_Identity=29.2682926829268, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23483; Mature: 23352

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTPAAVAPRNETVFVVDDDEAMRDSLTWLLEGNGYTVRTYRSAEEFLVDDKRGEGVGC
CCCCCCCCCCCCCEEEEEECCHHHHHHEEEEEECCCEEEEEECCHHHHHHCCCCCCCEEE
LILDVRMQGMSGPELQDRLLAENNRMPIVFVTGHGDVPLAVSTMKKGAVDFIEKPFDESE
EEEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHH
LRELVERMLSKARSEDSVAREQKAAKDLLGRLTTREQQVLERIVAGRLNKQIADDLGISI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEE
KTVEAHRANIMEKLNVNTVADLLRLALSRNA
EEEHHHHHHHHHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STTPAAVAPRNETVFVVDDDEAMRDSLTWLLEGNGYTVRTYRSAEEFLVDDKRGEGVGC
CCCCCCCCCCCCEEEEEECCHHHHHHEEEEEECCCEEEEEECCHHHHHHCCCCCCCEEE
LILDVRMQGMSGPELQDRLLAENNRMPIVFVTGHGDVPLAVSTMKKGAVDFIEKPFDESE
EEEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHH
LRELVERMLSKARSEDSVAREQKAAKDLLGRLTTREQQVLERIVAGRLNKQIADDLGISI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEE
KTVEAHRANIMEKLNVNTVADLLRLALSRNA
EEEHHHHHHHHHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]