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The map label for this gene is folD

Identifier: 187929030

GI number: 187929030

Start: 2060538

End: 2061407

Strand: Direct

Name: folD

Synonym: Rpic_1950

Alternate gene names: 187929030

Gene position: 2060538-2061407 (Clockwise)

Preceding gene: 187929029

Following gene: 187929031

Centisome position: 52.26

GC content: 67.36

Gene sequence:

>870_bases
ATGACCGCCCAACTCATTGACGGCAACGCGCTTGCCAAACAACTCCGCGCCGAGGCCGCACAGCGCGCCGCCGCCCTGAC
CGCGCGCGGCCACCAGCCCGGCCTCGCCGTCATCCTCGTCGGCGAAGACCCGGCCAGCCAGGTCTACGTGCGCAACAAGA
TCAAGGCCTGCGAAGACAACGGCTTTCTGTCCAGCTTCGACCGCTACCCCGCCGACCTGTCCGAAGCCGACTTGCTCGGC
CGCATCGACGCCCTGAACCGCGACCCGCGCATCCACGGCATCCTGGTCCAGCTGCCGCTGCCCAAGCACATCGACAGCCA
CAAGGTGCTCGAAGCCATCGCGCCCGAGAAAGACGTGGACGGTTTCCACGTGGCCAATGCCGGCGCCCTGATGACCGGCG
CCCCGCTGTTCCGCCCGTGCACGCCGTACGGCTGCATGAAGATGCTGGAATCGATCAACTACCCGGTGCGCGGCGCCAAT
GCGGTGGTGGTCGGCGCCTCGAACATCGTCGGCAAACCGATGGCGATGCTGCTGCTGCAATCCGGCGCCACCATCACGAT
CTGCAACAGCAAGACGCGTGACCTTGCCGCCCACACGCGTGAGGCCGACATCATCGTGGCCGCCGTCGGGCGCCGCAACA
TCATTACCGCCGACATGGTCAAGCCGGGCGCGGTCGTCATCGACGTGGGCATGAACCGCGACGACGCCGGCAAGCTGTGC
GGCGACGTCGACTTTGCCGGCGTCAAGGAAGTCGCCGGCTACATCACGCCGGTGCCGGGCGGTGTCGGCCCGATGACGAT
CACCATGCTGCTGATCAACACCCTGGAAGCCGCCGAGCGCGCTGCTGAAGGAGCCGCGCTGGCGGCGTAA

Upstream 100 bases:

>100_bases
CCTGATTCGGCTGCAGGAAAAACCAAGCGATCATCCGGGCGGCCGGAAGGGAGGCGGCGATTTATAATCGCGGTTTGCCC
TCTCCCTCCTCCGCCCAGCC

Downstream 100 bases:

>100_bases
GCTTCACCCTGATCCAGTTGGAAACGGAAAAAGCGTCCCCACCTGTTGGATCGGACGCTTTTTTTGTCCCCCGAGTTTCT
CTACCGAGCCCGCCATGACC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTAQLIDGNALAKQLRAEAAQRAAALTARGHQPGLAVILVGEDPASQVYVRNKIKACEDNGFLSSFDRYPADLSEADLLG
RIDALNRDPRIHGILVQLPLPKHIDSHKVLEAIAPEKDVDGFHVANAGALMTGAPLFRPCTPYGCMKMLESINYPVRGAN
AVVVGASNIVGKPMAMLLLQSGATITICNSKTRDLAAHTREADIIVAAVGRRNIITADMVKPGAVVIDVGMNRDDAGKLC
GDVDFAGVKEVAGYITPVPGGVGPMTITMLLINTLEAAERAAEGAALAA

Sequences:

>Translated_289_residues
MTAQLIDGNALAKQLRAEAAQRAAALTARGHQPGLAVILVGEDPASQVYVRNKIKACEDNGFLSSFDRYPADLSEADLLG
RIDALNRDPRIHGILVQLPLPKHIDSHKVLEAIAPEKDVDGFHVANAGALMTGAPLFRPCTPYGCMKMLESINYPVRGAN
AVVVGASNIVGKPMAMLLLQSGATITICNSKTRDLAAHTREADIIVAAVGRRNIITADMVKPGAVVIDVGMNRDDAGKLC
GDVDFAGVKEVAGYITPVPGGVGPMTITMLLINTLEAAERAAEGAALAA
>Mature_288_residues
TAQLIDGNALAKQLRAEAAQRAAALTARGHQPGLAVILVGEDPASQVYVRNKIKACEDNGFLSSFDRYPADLSEADLLGR
IDALNRDPRIHGILVQLPLPKHIDSHKVLEAIAPEKDVDGFHVANAGALMTGAPLFRPCTPYGCMKMLESINYPVRGANA
VVVGASNIVGKPMAMLLLQSGATITICNSKTRDLAAHTREADIIVAAVGRRNIITADMVKPGAVVIDVGMNRDDAGKLCG
DVDFAGVKEVAGYITPVPGGVGPMTITMLLINTLEAAERAAEGAALAA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=47.2789115646259, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=46.2585034013605, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI222136639, Length=293, Percent_Identity=41.296928327645, Blast_Score=225, Evalue=3e-59,
Organism=Homo sapiens, GI36796743, Length=223, Percent_Identity=29.5964125560538, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=54.3165467625899, Blast_Score=287, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI17568735, Length=296, Percent_Identity=42.5675675675676, Blast_Score=213, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=46.6898954703833, Blast_Score=261, Evalue=7e-71,
Organism=Saccharomyces cerevisiae, GI6321643, Length=306, Percent_Identity=42.156862745098, Blast_Score=234, Evalue=9e-63,
Organism=Saccharomyces cerevisiae, GI6322933, Length=295, Percent_Identity=26.1016949152542, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=48.6394557823129, Blast_Score=257, Evalue=6e-69,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=48.6394557823129, Blast_Score=257, Evalue=7e-69,
Organism=Drosophila melanogaster, GI62472483, Length=292, Percent_Identity=45.2054794520548, Blast_Score=250, Evalue=6e-67,
Organism=Drosophila melanogaster, GI45551871, Length=292, Percent_Identity=45.2054794520548, Blast_Score=250, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24645718, Length=292, Percent_Identity=45.2054794520548, Blast_Score=250, Evalue=7e-67,
Organism=Drosophila melanogaster, GI17137370, Length=292, Percent_Identity=45.2054794520548, Blast_Score=250, Evalue=7e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_RALPJ (B2UFY4)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001899517.1
- ProteinModelPortal:   B2UFY4
- SMR:   B2UFY4
- GeneID:   6289622
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_1950
- HOGENOM:   HBG328751
- OMA:   TYVRNKQ
- ProtClustDB:   PRK14189
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30291; Mature: 30160

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQLIDGNALAKQLRAEAAQRAAALTARGHQPGLAVILVGEDPASQVYVRNKIKACEDN
CCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCC
GFLSSFDRYPADLSEADLLGRIDALNRDPRIHGILVQLPLPKHIDSHKVLEAIAPEKDVD
CCHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
GFHVANAGALMTGAPLFRPCTPYGCMKMLESINYPVRGANAVVVGASNIVGKPMAMLLLQ
CEEEECCCCCEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCHHCCCHHHHHHHH
SGATITICNSKTRDLAAHTREADIIVAAVGRRNIITADMVKPGAVVIDVGMNRDDAGKLC
CCCEEEEECCCCHHHHHHCCCCCEEEEECCCCCEEECCCCCCCEEEEEECCCCCCHHHHC
GDVDFAGVKEVAGYITPVPGGVGPMTITMLLINTLEAAERAAEGAALAA
CCCCCCCHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TAQLIDGNALAKQLRAEAAQRAAALTARGHQPGLAVILVGEDPASQVYVRNKIKACEDN
CCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCC
GFLSSFDRYPADLSEADLLGRIDALNRDPRIHGILVQLPLPKHIDSHKVLEAIAPEKDVD
CCHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
GFHVANAGALMTGAPLFRPCTPYGCMKMLESINYPVRGANAVVVGASNIVGKPMAMLLLQ
CEEEECCCCCEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCHHCCCHHHHHHHH
SGATITICNSKTRDLAAHTREADIIVAAVGRRNIITADMVKPGAVVIDVGMNRDDAGKLC
CCCEEEEECCCCHHHHHHCCCCCEEEEECCCCCEEECCCCCCCEEEEEECCCCCCHHHHC
GDVDFAGVKEVAGYITPVPGGVGPMTITMLLINTLEAAERAAEGAALAA
CCCCCCCHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA