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The map label for this gene is rimO

Identifier: 187928991

GI number: 187928991

Start: 2015478

End: 2016839

Strand: Reverse

Name: rimO

Synonym: Rpic_1911

Alternate gene names: 187928991

Gene position: 2016839-2015478 (Counterclockwise)

Preceding gene: 187928992

Following gene: 187928990

Centisome position: 51.16

GC content: 64.98

Gene sequence:

>1362_bases
ATGTCCGACGTCAGTACCCAGAGCCCCAAGGTGGGGTTCGTCAGTCTCGGTTGTCCCAAGGCCTTGGTCGATTCCGAGCA
GATCATCACCCAACTGCGCGCGGAGGGGTATGAGATTTCCGGCACGTACGGCGGGGCGGACCTGGTGGTCGTCAACACCT
GCGGCTTCATCGACGAGGCTGTGCAGGAAAGCCTGGATGCGATTGGCGAGGCGCTGGCCGAGAACGGCAAGGTGATCGTG
ACCGGCTGCCTGGGTGCCAAGAAGGACGCTTCGGGCCAGGACATCATCACCAGCGTGCACCCGAAGGTGCTGGCTGTGAC
GGGCCCCCATGCGCTGGGCGAGGTGATGGAAGCGGTGCACACGCACCTGCCCAAGCCGCACGACCCGTTCATTGACCTGG
TGCCGCCGCAGGGCATCAAGCTCACGCCGAAGCACTACGCGTATCTGAAGATTTCCGAGGGCTGCAACCACCGGTGCAGC
TTCTGCATCATCCCGTCGATGCGCGGCGATCTCGTTTCGCGCCCGGTGGCCGAAGTGATGCTGGAGGCGGAAAACCTGCT
GAAGGCCGGCGTGAAGGAACTGCTCGTTATCTCGCAGGACACCAGCGCTTATGGCGTCGATGTCAAATTCCGCATGGGCT
TCTGGAACGGCCGCCCGCTGAAGACGCGCATGACCGAGCTGGTGGGCGCGCTCGGCGAATTGGCGTCGCAGTACGGCGCC
TGGGTGCGCCTGCACTACGTCTACCCCTATCCGAGCGTGGATGAGGTGATGCCCCTGATGGCTGAGGGCAAGGTCCTTCC
CTATCTGGACGTGCCGCTGCAGCACGCCCACCCGGACGTGCTCAAGCGCATGAAGCGCCCCGCCAACGCCGAGAAGACGC
TGGACCGCATCCGCGCCTGGCGCGAGGTGTGCCCGGAGCTGACCATCCGCAGCACGTTCATCGCGGGTTTCCCGGGTGAA
ACGGAGGAAGAGTTCCAAACGCTGCTCGACTTCATTGCCGAGGCCGAACTGGACCGCGTGGGCTGCTTCGCGTATTCGCC
GGTCGAGGGCGCGACCGCCAACGACCTGCCGGGCGCATTGCCCGATGAGGTGCGCGAGGAGCGCCGCGCGCGCTTCATGG
AAGTCGCCGAGCGCGTGTCTGCGCGCGGTCTGCAGCGCAAGGTTGGCAAGTCGCTGCGCGTGCTTGTGGACGAAGTGAAT
CAGGACGGCGGCATCGGCCGTTCATCGGCGGATGCCCCGGAGATTGACGGCCTCGTCTATATCGCGCCGCCGTCGAAGCC
GTACAAGCGCTATAAGGCGGGCGATTTCGTGTCGGTCAAGATTACCGGCGCGGATGGCCACGACCTGTGGGGCGAGGTCT
GA

Upstream 100 bases:

>100_bases
TCGGCTTGGCCGCCCAGGCGCGGCGAACGGTTAAAATACGCAACCGCGCGGCACACCCCACGTGCGCAGCGCAAATCCTC
TCCCACATCAGGTTGTTCCC

Downstream 100 bases:

>100_bases
TCCTCTTAGGTAGTCGTACCTAAATTTTGCGAACGGTCGTGCGTTTATACTGGCCGCCATCCAATTTCGATGGCACAGGA
GACAGCAATGGCACGTGAAG

Product: 30S ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSDVSTQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYGGADLVVVNTCGFIDEAVQESLDAIGEALAENGKVIV
TGCLGAKKDASGQDIITSVHPKVLAVTGPHALGEVMEAVHTHLPKPHDPFIDLVPPQGIKLTPKHYAYLKISEGCNHRCS
FCIIPSMRGDLVSRPVAEVMLEAENLLKAGVKELLVISQDTSAYGVDVKFRMGFWNGRPLKTRMTELVGALGELASQYGA
WVRLHYVYPYPSVDEVMPLMAEGKVLPYLDVPLQHAHPDVLKRMKRPANAEKTLDRIRAWREVCPELTIRSTFIAGFPGE
TEEEFQTLLDFIAEAELDRVGCFAYSPVEGATANDLPGALPDEVREERRARFMEVAERVSARGLQRKVGKSLRVLVDEVN
QDGGIGRSSADAPEIDGLVYIAPPSKPYKRYKAGDFVSVKITGADGHDLWGEV

Sequences:

>Translated_453_residues
MSDVSTQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYGGADLVVVNTCGFIDEAVQESLDAIGEALAENGKVIV
TGCLGAKKDASGQDIITSVHPKVLAVTGPHALGEVMEAVHTHLPKPHDPFIDLVPPQGIKLTPKHYAYLKISEGCNHRCS
FCIIPSMRGDLVSRPVAEVMLEAENLLKAGVKELLVISQDTSAYGVDVKFRMGFWNGRPLKTRMTELVGALGELASQYGA
WVRLHYVYPYPSVDEVMPLMAEGKVLPYLDVPLQHAHPDVLKRMKRPANAEKTLDRIRAWREVCPELTIRSTFIAGFPGE
TEEEFQTLLDFIAEAELDRVGCFAYSPVEGATANDLPGALPDEVREERRARFMEVAERVSARGLQRKVGKSLRVLVDEVN
QDGGIGRSSADAPEIDGLVYIAPPSKPYKRYKAGDFVSVKITGADGHDLWGEV
>Mature_452_residues
SDVSTQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYGGADLVVVNTCGFIDEAVQESLDAIGEALAENGKVIVT
GCLGAKKDASGQDIITSVHPKVLAVTGPHALGEVMEAVHTHLPKPHDPFIDLVPPQGIKLTPKHYAYLKISEGCNHRCSF
CIIPSMRGDLVSRPVAEVMLEAENLLKAGVKELLVISQDTSAYGVDVKFRMGFWNGRPLKTRMTELVGALGELASQYGAW
VRLHYVYPYPSVDEVMPLMAEGKVLPYLDVPLQHAHPDVLKRMKRPANAEKTLDRIRAWREVCPELTIRSTFIAGFPGET
EEEFQTLLDFIAEAELDRVGCFAYSPVEGATANDLPGALPDEVREERRARFMEVAERVSARGLQRKVGKSLRVLVDEVNQ
DGGIGRSSADAPEIDGLVYIAPPSKPYKRYKAGDFVSVKITGADGHDLWGEV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=488, Percent_Identity=25.4098360655738, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI93277076, Length=411, Percent_Identity=27.007299270073, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI28872782, Length=488, Percent_Identity=25.4098360655738, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1787057, Length=452, Percent_Identity=65.7079646017699, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI1786882, Length=405, Percent_Identity=25.9259259259259, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71996771, Length=304, Percent_Identity=28.2894736842105, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21356207, Length=488, Percent_Identity=26.4344262295082, Blast_Score=141, Evalue=9e-34,
Organism=Drosophila melanogaster, GI19922432, Length=464, Percent_Identity=25.8620689655172, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_RALPJ (B2UFU5)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001899478.1
- ProteinModelPortal:   B2UFU5
- SMR:   B2UFU5
- GeneID:   6289698
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_1911
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   PRK14862
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49423; Mature: 49292

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVSTQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYGGADLVVVNTCGFIDEA
CCCCCCCCCCCEEEECCCCHHHHCHHHHHHHHHHCCEEEECCCCCCEEEEEECCCHHHHH
VQESLDAIGEALAENGKVIVTGCLGAKKDASGQDIITSVHPKVLAVTGPHALGEVMEAVH
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHCCCCEEEEEECCHHHHHHHHHHH
THLPKPHDPFIDLVPPQGIKLTPKHYAYLKISEGCNHRCSFCIIPSMRGDLVSRPVAEVM
HHCCCCCCCCEECCCCCCCEECCCCEEEEEECCCCCCCCCEEEECCCCCHHHHCHHHHHH
LEAENLLKAGVKELLVISQDTSAYGVDVKFRMGFWNGRPLKTRMTELVGALGELASQYGA
HHHHHHHHHHHHHEEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCC
WVRLHYVYPYPSVDEVMPLMAEGKVLPYLDVPLQHAHPDVLKRMKRPANAEKTLDRIRAW
EEEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHH
REVCPELTIRSTFIAGFPGETEEEFQTLLDFIAEAELDRVGCFAYSPVEGATANDLPGAL
HHHCCCCEEHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCC
PDEVREERRARFMEVAERVSARGLQRKVGKSLRVLVDEVNQDGGIGRSSADAPEIDGLVY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEE
IAPPSKPYKRYKAGDFVSVKITGADGHDLWGEV
ECCCCCHHHHCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SDVSTQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYGGADLVVVNTCGFIDEA
CCCCCCCCCCEEEECCCCHHHHCHHHHHHHHHHCCEEEECCCCCCEEEEEECCCHHHHH
VQESLDAIGEALAENGKVIVTGCLGAKKDASGQDIITSVHPKVLAVTGPHALGEVMEAVH
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHCCCCEEEEEECCHHHHHHHHHHH
THLPKPHDPFIDLVPPQGIKLTPKHYAYLKISEGCNHRCSFCIIPSMRGDLVSRPVAEVM
HHCCCCCCCCEECCCCCCCEECCCCEEEEEECCCCCCCCCEEEECCCCCHHHHCHHHHHH
LEAENLLKAGVKELLVISQDTSAYGVDVKFRMGFWNGRPLKTRMTELVGALGELASQYGA
HHHHHHHHHHHHHEEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCC
WVRLHYVYPYPSVDEVMPLMAEGKVLPYLDVPLQHAHPDVLKRMKRPANAEKTLDRIRAW
EEEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHH
REVCPELTIRSTFIAGFPGETEEEFQTLLDFIAEAELDRVGCFAYSPVEGATANDLPGAL
HHHCCCCEEHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCC
PDEVREERRARFMEVAERVSARGLQRKVGKSLRVLVDEVNQDGGIGRSSADAPEIDGLVY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEE
IAPPSKPYKRYKAGDFVSVKITGADGHDLWGEV
ECCCCCHHHHCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA