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The map label for this gene is dusC [H]

Identifier: 187928992

GI number: 187928992

Start: 2016906

End: 2017862

Strand: Reverse

Name: dusC [H]

Synonym: Rpic_1912

Alternate gene names: 187928992

Gene position: 2017862-2016906 (Counterclockwise)

Preceding gene: 187928993

Following gene: 187928991

Centisome position: 51.18

GC content: 66.46

Gene sequence:

>957_bases
ATGGAAGGGGTCGCGGATTACGTCATGCGTGACGTGCTGACGCAAGCCGGCGGCTACGACGGATGCGTCTCGGAGTTTGT
CCGGGTGACGGGCTCGTTGCTCCCCGCGCGGACTTATGAGCGTGAGACGCCCGAGATCCGCAACGGCGGCTATACCGCCA
GCGGCACGCCGATGACCATCCAACTCCTCGGTAGCGATCCGGAATGGCTGGCCCGCAATGCGGCATACGCGGCAACCCTC
ACGCCACATGGCATCGACCTCAACTTCGGCTGCCCCGCCAAGGTCGTGAACCGGCATGGCGGCGGCGCCATGCTGCTTGC
CACGCCGGAAGTGCTGCACGACATCGTGCGCGCCGTGCGGGCCGCGGTGCCGGCCAACATTTCGGTGACGGCGAAGATGC
GGCTTGGCGTTTCCGATACTTCCCGTGCGATTGACTGTGCGACGGCCCTGGCGGAAGGCGGTGCGGAATCGCTCGTCGTG
CATGCCCGCACGCGCGATCACGGCTACCGTCCGCCCGCCCACTGGGACTGGATTGCACGCATCGCCGATGCCGTGAGCAT
CCCCGTGACCGCCAACGGCGAAGTCTGGACCGTGGCTGACTGGGAGCGCTGCCGCGCCGTCAGTGGCTGCAGTGACGTGA
TGATCGGACGTGGCGCCGTGTCCGACCCATTCCTGGCCCAGCGCATCCGCGCGCAGATGGACCAGGCGCCGTCCGCGGCG
GAATGGCCGATCGTGCTGGGCTATCTCGCCGACTACTTGAAAAAGCTCCATGCGCGCATCTCTGCCAAGCACGAGCATGG
CCGCGTCAAACTGTGGCTGAGCTATCTGAAGCGGACGTGGCCCCAGGCCGCCGAGCTGCACGATGCCATTCGCAGGTTGC
AGGACGCGCTGGAAATCGAGCGCGTGATTGAGCAGATGCAGCTTCGGCTTGGCCGCCCAGGCGCGGCGAACGGTTAA

Upstream 100 bases:

>100_bases
GATCACGATTCCCTCCCCGGTCTCCCATGTTTGATGTCCGCCCCCGGCGTGTGCGCCGGCTGTCTTCCGTTTTCGCATGA
GCTGCCTTTTGCTTGCCCCG

Downstream 100 bases:

>100_bases
AATACGCAACCGCGCGGCACACCCCACGTGCGCAGCGCAAATCCTCTCCCACATCAGGTTGTTCCCATGTCCGACGTCAG
TACCCAGAGCCCCAAGGTGG

Product: dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MEGVADYVMRDVLTQAGGYDGCVSEFVRVTGSLLPARTYERETPEIRNGGYTASGTPMTIQLLGSDPEWLARNAAYAATL
TPHGIDLNFGCPAKVVNRHGGGAMLLATPEVLHDIVRAVRAAVPANISVTAKMRLGVSDTSRAIDCATALAEGGAESLVV
HARTRDHGYRPPAHWDWIARIADAVSIPVTANGEVWTVADWERCRAVSGCSDVMIGRGAVSDPFLAQRIRAQMDQAPSAA
EWPIVLGYLADYLKKLHARISAKHEHGRVKLWLSYLKRTWPQAAELHDAIRRLQDALEIERVIEQMQLRLGRPGAANG

Sequences:

>Translated_318_residues
MEGVADYVMRDVLTQAGGYDGCVSEFVRVTGSLLPARTYERETPEIRNGGYTASGTPMTIQLLGSDPEWLARNAAYAATL
TPHGIDLNFGCPAKVVNRHGGGAMLLATPEVLHDIVRAVRAAVPANISVTAKMRLGVSDTSRAIDCATALAEGGAESLVV
HARTRDHGYRPPAHWDWIARIADAVSIPVTANGEVWTVADWERCRAVSGCSDVMIGRGAVSDPFLAQRIRAQMDQAPSAA
EWPIVLGYLADYLKKLHARISAKHEHGRVKLWLSYLKRTWPQAAELHDAIRRLQDALEIERVIEQMQLRLGRPGAANG
>Mature_318_residues
MEGVADYVMRDVLTQAGGYDGCVSEFVRVTGSLLPARTYERETPEIRNGGYTASGTPMTIQLLGSDPEWLARNAAYAATL
TPHGIDLNFGCPAKVVNRHGGGAMLLATPEVLHDIVRAVRAAVPANISVTAKMRLGVSDTSRAIDCATALAEGGAESLVV
HARTRDHGYRPPAHWDWIARIADAVSIPVTANGEVWTVADWERCRAVSGCSDVMIGRGAVSDPFLAQRIRAQMDQAPSAA
EWPIVLGYLADYLKKLHARISAKHEHGRVKLWLSYLKRTWPQAAELHDAIRRLQDALEIERVIEQMQLRLGRPGAANG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=180, Percent_Identity=31.6666666666667, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI239788483, Length=227, Percent_Identity=28.1938325991189, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI239788462, Length=227, Percent_Identity=28.1938325991189, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI40807366, Length=255, Percent_Identity=27.843137254902, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI8923374, Length=157, Percent_Identity=31.2101910828025, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788462, Length=310, Percent_Identity=41.9354838709677, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI1789660, Length=254, Percent_Identity=33.4645669291339, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI145693211, Length=187, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=188, Percent_Identity=32.9787234042553, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI25144369, Length=171, Percent_Identity=30.9941520467836, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17510279, Length=149, Percent_Identity=33.5570469798658, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=173, Percent_Identity=24.8554913294798, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323437, Length=203, Percent_Identity=28.0788177339901, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323560, Length=171, Percent_Identity=26.9005847953216, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323433, Length=213, Percent_Identity=26.2910798122066, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24585320, Length=231, Percent_Identity=32.034632034632, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19921524, Length=178, Percent_Identity=30.8988764044944, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24580595, Length=171, Percent_Identity=30.9941520467836, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920448, Length=171, Percent_Identity=30.9941520467836, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45549423, Length=139, Percent_Identity=35.2517985611511, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34605; Mature: 34605

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGVADYVMRDVLTQAGGYDGCVSEFVRVTGSLLPARTYERETPEIRNGGYTASGTPMTI
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCEECCCCCEEE
QLLGSDPEWLARNAAYAATLTPHGIDLNFGCPAKVVNRHGGGAMLLATPEVLHDIVRAVR
EEECCCHHHHHCCCCEEEEECCCCEECCCCCCHHHHHCCCCCEEEEECHHHHHHHHHHHH
AAVPANISVTAKMRLGVSDTSRAIDCATALAEGGAESLVVHARTRDHGYRPPAHWDWIAR
HHCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHH
IADAVSIPVTANGEVWTVADWERCRAVSGCSDVMIGRGAVSDPFLAQRIRAQMDQAPSAA
HHHHHCCCEECCCCEEEECCHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCC
EWPIVLGYLADYLKKLHARISAKHEHGRVKLWLSYLKRTWPQAAELHDAIRRLQDALEIE
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RVIEQMQLRLGRPGAANG
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEGVADYVMRDVLTQAGGYDGCVSEFVRVTGSLLPARTYERETPEIRNGGYTASGTPMTI
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCEECCCCCEEE
QLLGSDPEWLARNAAYAATLTPHGIDLNFGCPAKVVNRHGGGAMLLATPEVLHDIVRAVR
EEECCCHHHHHCCCCEEEEECCCCEECCCCCCHHHHHCCCCCEEEEECHHHHHHHHHHHH
AAVPANISVTAKMRLGVSDTSRAIDCATALAEGGAESLVVHARTRDHGYRPPAHWDWIAR
HHCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHH
IADAVSIPVTANGEVWTVADWERCRAVSGCSDVMIGRGAVSDPFLAQRIRAQMDQAPSAA
HHHHHCCCEECCCCEEEECCHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCC
EWPIVLGYLADYLKKLHARISAKHEHGRVKLWLSYLKRTWPQAAELHDAIRRLQDALEIE
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RVIEQMQLRLGRPGAANG
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]