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The map label for this gene is thlA [H]

Identifier: 187928990

GI number: 187928990

Start: 2014206

End: 2015390

Strand: Reverse

Name: thlA [H]

Synonym: Rpic_1910

Alternate gene names: 187928990

Gene position: 2015390-2014206 (Counterclockwise)

Preceding gene: 187928991

Following gene: 187928989

Centisome position: 51.12

GC content: 65.57

Gene sequence:

>1185_bases
ATGGCACGTGAAGTGGTGGTGGTCAGCGGCGTTCGTACCGCAATCGGGACGTTTGGCGGCAGCTTGAAGGATGTGGCGCC
GTGCGAACTCGGCGCGCTCGTCGTGCGCGAGGCGCTGGCCCGCGCCGGGGTGAAGGGCGAGGACGTCGGCCACGTGGTGT
TTGGCCACGTGATCAACACCGAGCCGCGCGACATGTATCTCTCGCGTGTGGCCGCAGTCAACGGCGGGGTGTCGGCCGAA
ACGCCCGCATTCAACGTCAACCGCCTGTGCGGCTCTGGCCTCCAGGCCGTGGTGAGCGCAGCGCAAACCGTGCTGCTGGG
CGATGCCGATATCGCCATTGCCGGTGGCGCGGAAAGCATGAGCCGCGCGCCGTACCTGGCGCCTGCCGCCCGTTGGGGTT
CGCGCATGGGCGACGCCAAGATGGTCGACATGATGCTCGGCGCGCTGCACGACCCGTTCCACACGATCCACATGGGTGTG
ACGGCGGAAAACGTCGCCCGCGAATTCGGCATCAGCCGCGAGCAGCAAGATCAGACCGCGCTGGAGTCCCACCAGCGTGC
ATCGCGTGCCATCCAGGCCGGCTACTTCAAGGACCAGATCGTTCCGGTGACGATCAAGTCGCGCAAGGGCGATATCCAGT
TCGATACCGATGAGCACGTCCGCCACGACGCGACCATCGACGACATGACCAAGCTCAAGCCGGTCTTCACGAAAGAAAAC
GGTACGGTGACGGCGGGCAACGCTTCGGGCCTCAACGATGCAGGCGCGGCGCTGGTGCTGATGGAGCGTTCGGTGGCCGA
AAAGCGCGGCCTCAAGCCACTGGCCCGGCTGGTGTCGTACGGCCATGCGGGCGTGGATCCGAAGATCATGGGTATCGGCC
CGGTGCCGGCCACGCGCAAGGCGCTGGAGCGGGCAGGCCTGTCGGTCAAGGATCTGGATGTGATCGAAGCCAACGAGGCA
TTTGCCGCCCAGGCTTGTGCGGTGACGAAGGAACTCGGCCTGGACCCGGCCAAGGTCAACCCAAACGGTTCGGGCATTTC
GCTCGGCCACCCGATCGGCGCAACGGGTGCGCTTATCACGGTCAAGGCGTTGCACGAGCTGCAGCGCATCGGTGGTCGCT
ACGCGCTGGTGACCATGTGTATTGGTGGAGGACAGGGCATTGCGGCGGTGTTCGAGCGCATCTGA

Upstream 100 bases:

>100_bases
GGGCGAGGTCTGATCCTCTTAGGTAGTCGTACCTAAATTTTGCGAACGGTCGTGCGTTTATACTGGCCGCCATCCAATTT
CGATGGCACAGGAGACAGCA

Downstream 100 bases:

>100_bases
ACGCATTTTCTCGATTGAACGCCTGATTCGGCCAACCGGCGCGTTGCTTGCGCTGGTGCTGGCCGCAGGCGTGGCGCCGG
TGTCTGCCCAGACGCCGGCC

Product: beta-ketothiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAREVVVVSGVRTAIGTFGGSLKDVAPCELGALVVREALARAGVKGEDVGHVVFGHVINTEPRDMYLSRVAAVNGGVSAE
TPAFNVNRLCGSGLQAVVSAAQTVLLGDADIAIAGGAESMSRAPYLAPAARWGSRMGDAKMVDMMLGALHDPFHTIHMGV
TAENVAREFGISREQQDQTALESHQRASRAIQAGYFKDQIVPVTIKSRKGDIQFDTDEHVRHDATIDDMTKLKPVFTKEN
GTVTAGNASGLNDAGAALVLMERSVAEKRGLKPLARLVSYGHAGVDPKIMGIGPVPATRKALERAGLSVKDLDVIEANEA
FAAQACAVTKELGLDPAKVNPNGSGISLGHPIGATGALITVKALHELQRIGGRYALVTMCIGGGQGIAAVFERI

Sequences:

>Translated_394_residues
MAREVVVVSGVRTAIGTFGGSLKDVAPCELGALVVREALARAGVKGEDVGHVVFGHVINTEPRDMYLSRVAAVNGGVSAE
TPAFNVNRLCGSGLQAVVSAAQTVLLGDADIAIAGGAESMSRAPYLAPAARWGSRMGDAKMVDMMLGALHDPFHTIHMGV
TAENVAREFGISREQQDQTALESHQRASRAIQAGYFKDQIVPVTIKSRKGDIQFDTDEHVRHDATIDDMTKLKPVFTKEN
GTVTAGNASGLNDAGAALVLMERSVAEKRGLKPLARLVSYGHAGVDPKIMGIGPVPATRKALERAGLSVKDLDVIEANEA
FAAQACAVTKELGLDPAKVNPNGSGISLGHPIGATGALITVKALHELQRIGGRYALVTMCIGGGQGIAAVFERI
>Mature_393_residues
AREVVVVSGVRTAIGTFGGSLKDVAPCELGALVVREALARAGVKGEDVGHVVFGHVINTEPRDMYLSRVAAVNGGVSAET
PAFNVNRLCGSGLQAVVSAAQTVLLGDADIAIAGGAESMSRAPYLAPAARWGSRMGDAKMVDMMLGALHDPFHTIHMGVT
AENVAREFGISREQQDQTALESHQRASRAIQAGYFKDQIVPVTIKSRKGDIQFDTDEHVRHDATIDDMTKLKPVFTKENG
TVTAGNASGLNDAGAALVLMERSVAEKRGLKPLARLVSYGHAGVDPKIMGIGPVPATRKALERAGLSVKDLDVIEANEAF
AAQACAVTKELGLDPAKVNPNGSGISLGHPIGATGALITVKALHELQRIGGRYALVTMCIGGGQGIAAVFERI

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=391, Percent_Identity=49.3606138107417, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI167614485, Length=393, Percent_Identity=44.7837150127226, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI4501853, Length=399, Percent_Identity=44.3609022556391, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI4557237, Length=398, Percent_Identity=38.6934673366834, Blast_Score=272, Evalue=3e-73,
Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=35.7647058823529, Blast_Score=208, Evalue=7e-54,
Organism=Homo sapiens, GI194353979, Length=394, Percent_Identity=33.502538071066, Blast_Score=162, Evalue=4e-40,
Organism=Escherichia coli, GI1788554, Length=398, Percent_Identity=49.748743718593, Blast_Score=351, Evalue=4e-98,
Organism=Escherichia coli, GI87082165, Length=392, Percent_Identity=46.6836734693878, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1787663, Length=403, Percent_Identity=46.6501240694789, Blast_Score=331, Evalue=4e-92,
Organism=Escherichia coli, GI48994986, Length=402, Percent_Identity=41.044776119403, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1788683, Length=412, Percent_Identity=34.4660194174757, Blast_Score=216, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI133906874, Length=391, Percent_Identity=45.7800511508951, Blast_Score=349, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI25147385, Length=393, Percent_Identity=35.6234096692112, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17535921, Length=396, Percent_Identity=33.3333333333333, Blast_Score=221, Evalue=6e-58,
Organism=Caenorhabditis elegans, GI17551802, Length=424, Percent_Identity=32.5471698113208, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=28.1481481481481, Blast_Score=155, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6325229, Length=400, Percent_Identity=39.5, Blast_Score=276, Evalue=6e-75,
Organism=Saccharomyces cerevisiae, GI6322031, Length=403, Percent_Identity=38.4615384615385, Blast_Score=230, Evalue=4e-61,
Organism=Drosophila melanogaster, GI24655093, Length=392, Percent_Identity=45.4081632653061, Blast_Score=348, Evalue=3e-96,
Organism=Drosophila melanogaster, GI17648125, Length=392, Percent_Identity=43.3673469387755, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI24640423, Length=397, Percent_Identity=40.0503778337531, Blast_Score=274, Evalue=6e-74,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=32.5526932084309, Blast_Score=183, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41183; Mature: 41052

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAREVVVVSGVRTAIGTFGGSLKDVAPCELGALVVREALARAGVKGEDVGHVVFGHVINT
CCCCEEEECCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEHEECC
EPRDMYLSRVAAVNGGVSAETPAFNVNRLCGSGLQAVVSAAQTVLLGDADIAIAGGAESM
CHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHEEECCCCEEEECCHHHH
SRAPYLAPAARWGSRMGDAKMVDMMLGALHDPFHTIHMGVTAENVAREFGISREQQDQTA
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCHHHHHH
LESHQRASRAIQAGYFKDQIVPVTIKSRKGDIQFDTDEHVRHDATIDDMTKLKPVFTKEN
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCHHHHHCCCCHHHHHHCCCEEECCC
GTVTAGNASGLNDAGAALVLMERSVAEKRGLKPLARLVSYGHAGVDPKIMGIGPVPATRK
CEEEECCCCCCCCCCCEEEEHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCCCHHHH
ALERAGLSVKDLDVIEANEAFAAQACAVTKELGLDPAKVNPNGSGISLGHPIGATGALIT
HHHHCCCCEECCEEEECCHHHHHHHHHHHHHHCCCCCEECCCCCCEECCCCCCCCCHHHH
VKALHELQRIGGRYALVTMCIGGGQGIAAVFERI
HHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure 
AREVVVVSGVRTAIGTFGGSLKDVAPCELGALVVREALARAGVKGEDVGHVVFGHVINT
CCCEEEECCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEHEECC
EPRDMYLSRVAAVNGGVSAETPAFNVNRLCGSGLQAVVSAAQTVLLGDADIAIAGGAESM
CHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHEEECCCCEEEECCHHHH
SRAPYLAPAARWGSRMGDAKMVDMMLGALHDPFHTIHMGVTAENVAREFGISREQQDQTA
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCHHHHHH
LESHQRASRAIQAGYFKDQIVPVTIKSRKGDIQFDTDEHVRHDATIDDMTKLKPVFTKEN
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCHHHHHCCCCHHHHHHCCCEEECCC
GTVTAGNASGLNDAGAALVLMERSVAEKRGLKPLARLVSYGHAGVDPKIMGIGPVPATRK
CEEEECCCCCCCCCCCEEEEHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCCCHHHH
ALERAGLSVKDLDVIEANEAFAAQACAVTKELGLDPAKVNPNGSGISLGHPIGATGALIT
HHHHCCCCEECCEEEECCHHHHHHHHHHHHHHCCCCCEECCCCCCEECCCCCCCCCHHHH
VKALHELQRIGGRYALVTMCIGGGQGIAAVFERI
HHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]