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The map label for this gene is tig [H]

Identifier: 187928951

GI number: 187928951

Start: 1970323

End: 1971666

Strand: Reverse

Name: tig [H]

Synonym: Rpic_1870

Alternate gene names: 187928951

Gene position: 1971666-1970323 (Counterclockwise)

Preceding gene: 187928952

Following gene: 187928950

Centisome position: 50.01

GC content: 60.94

Gene sequence:

>1344_bases
ATGTCGACTATCGAAAACCTCGGCAAGCTTGATCGCAAAGTGACCCTGGCTGTCCCGAAGGCCGAAGTGGAGCAAGAGAA
GCAAAGCCGTCTGGCGCGTCTGTCCAAGACCGTCAAGATGTCCGGCTTCCGCCCGGGCAAGGTCCCGATGAAGATGGTCG
AGAAGCAATACGGCCAACAGGTCGAATTCGAGGTGCGCTACGACAAGGCCGCCCGTCAGTTCTTTGACATCACCAACGAA
CAAGGCGTGAAGGTTGCCGGCCAACCGCGCTTCGAGCTGAAGACCGAAGGCGTGGCCGATGACCAGGTCGCGTTCGACGC
CACCTTCGAGGTGTATCCGGAAGTCAAGCTCGGCGATCTGGCCAGCGCGGAACTGACCCGCACCAAGACCGAAATCACCG
ATGCCGAGATCGACAAGACCCTCGACATCCTGCGCAAGCAACGCGTGCACTACCACCCGCGCGGCGAAGCCGGCGAGCAT
GGTGACGGCGGTGCAGCCGTTGCCCAGGACGGCGACCGCGTGACGGTCGATTTCGTCGGCACGATTGACGGTGTGGAATT
TGCCGGTGGCAAGGCTGAAGGTTTTAGCTTCGTGCTGGGCGAAGGCCGCATGTTGCCCGAGTTCGAGCAAGCCACGCTGG
GCCTGAAGCAAGGCGAGTCGAAGAAATTCCCGCTGGCATTCCCCGAGGACTACCACGGCAAGGATGTCGCGGGTAAGACG
GCTGAATTCACCGTCACGCTCAAGCAAGTCGAGTGGGCGCACCTGCCGGAAGTCAACGAAGCGTTTGCCCAATCGCTGGG
CATCGCCGACGGCAACCTGGACAAGATGCGCGCCGATATCCGCGAGAACCTCGAGCGTGAAGTCAAGCGCCGCACGCACG
CGCTGCTCAAGGACCAAGTCATGGAAGCGCTGCTGAAGGTGGCAGAGCTGGATGTGCCGAAGTCGCTGGTCGAGCAAGAC
CAAGAGCGCCTGGTTGAAATGGCTCGCCGTGACCTCGAATCGCGCGGCATGCCGAACGCCAAGAACATGCCGATCCCGGC
CGAGATGTTCACGCAGCAAGCTGAGCGCCGCGTGAAGCTGGGCCTGGTGCTGGCCGAAGTCGTGAAGGCCAACGGCCTGG
AAGCAAAGCCGGAGCAGATCAAGGCCGAGATCGAAGAGTTCGCCAAGAGCTACGAAGATCCGAAGGAAGTCATCCGCTGG
TACTACGGCGACCAACAGCGCCTGGCCGAGATGGAAGCCTACGTGCTCGAAAACAACGTGGTAAATTTTGTGTGCGACAA
GGCCAAAGTCACTGACAAGGCCGTGTCGTTCGAAGAGCTGACTGCCACGCCGGCGCAAGGCTGA

Upstream 100 bases:

>100_bases
GCATTCCTGCAAGCCCGGCAAGACCAAGAACTTTGAACCACGGCTGCTGCTGGCGCTGCGCCGTTCGACGCAACCATCTT
CTGAATCTGGAATTACGACC

Downstream 100 bases:

>100_bases
TTCGATCGATATAGCTCCCGAAATAAGCACCGGAGAACCGCATGATCCGCAATGAACTGATCGACCAACTTGCCCGCACC
CAGGCTTCCGCGCTCGAAAC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSTIENLGKLDRKVTLAVPKAEVEQEKQSRLARLSKTVKMSGFRPGKVPMKMVEKQYGQQVEFEVRYDKAARQFFDITNE
QGVKVAGQPRFELKTEGVADDQVAFDATFEVYPEVKLGDLASAELTRTKTEITDAEIDKTLDILRKQRVHYHPRGEAGEH
GDGGAAVAQDGDRVTVDFVGTIDGVEFAGGKAEGFSFVLGEGRMLPEFEQATLGLKQGESKKFPLAFPEDYHGKDVAGKT
AEFTVTLKQVEWAHLPEVNEAFAQSLGIADGNLDKMRADIRENLEREVKRRTHALLKDQVMEALLKVAELDVPKSLVEQD
QERLVEMARRDLESRGMPNAKNMPIPAEMFTQQAERRVKLGLVLAEVVKANGLEAKPEQIKAEIEEFAKSYEDPKEVIRW
YYGDQQRLAEMEAYVLENNVVNFVCDKAKVTDKAVSFEELTATPAQG

Sequences:

>Translated_447_residues
MSTIENLGKLDRKVTLAVPKAEVEQEKQSRLARLSKTVKMSGFRPGKVPMKMVEKQYGQQVEFEVRYDKAARQFFDITNE
QGVKVAGQPRFELKTEGVADDQVAFDATFEVYPEVKLGDLASAELTRTKTEITDAEIDKTLDILRKQRVHYHPRGEAGEH
GDGGAAVAQDGDRVTVDFVGTIDGVEFAGGKAEGFSFVLGEGRMLPEFEQATLGLKQGESKKFPLAFPEDYHGKDVAGKT
AEFTVTLKQVEWAHLPEVNEAFAQSLGIADGNLDKMRADIRENLEREVKRRTHALLKDQVMEALLKVAELDVPKSLVEQD
QERLVEMARRDLESRGMPNAKNMPIPAEMFTQQAERRVKLGLVLAEVVKANGLEAKPEQIKAEIEEFAKSYEDPKEVIRW
YYGDQQRLAEMEAYVLENNVVNFVCDKAKVTDKAVSFEELTATPAQG
>Mature_446_residues
STIENLGKLDRKVTLAVPKAEVEQEKQSRLARLSKTVKMSGFRPGKVPMKMVEKQYGQQVEFEVRYDKAARQFFDITNEQ
GVKVAGQPRFELKTEGVADDQVAFDATFEVYPEVKLGDLASAELTRTKTEITDAEIDKTLDILRKQRVHYHPRGEAGEHG
DGGAAVAQDGDRVTVDFVGTIDGVEFAGGKAEGFSFVLGEGRMLPEFEQATLGLKQGESKKFPLAFPEDYHGKDVAGKTA
EFTVTLKQVEWAHLPEVNEAFAQSLGIADGNLDKMRADIRENLEREVKRRTHALLKDQVMEALLKVAELDVPKSLVEQDQ
ERLVEMARRDLESRGMPNAKNMPIPAEMFTQQAERRVKLGLVLAEVVKANGLEAKPEQIKAEIEEFAKSYEDPKEVIRWY
YGDQQRLAEMEAYVLENNVVNFVCDKAKVTDKAVSFEELTATPAQG

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=439, Percent_Identity=38.496583143508, Blast_Score=306, Evalue=1e-84,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 50041; Mature: 49910

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIENLGKLDRKVTLAVPKAEVEQEKQSRLARLSKTVKMSGFRPGKVPMKMVEKQYGQQ
CCCHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
VEFEVRYDKAARQFFDITNEQGVKVAGQPRFELKTEGVADDQVAFDATFEVYPEVKLGDL
EEEEEEHHHHHHHHHHCCCCCCCEECCCCCEEEECCCCCCCCEEEEEEEEECCCCCCCCC
ASAELTRTKTEITDAEIDKTLDILRKQRVHYHPRGEAGEHGDGGAAVAQDGDRVTVDFVG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEEEEE
TIDGVEFAGGKAEGFSFVLGEGRMLPEFEQATLGLKQGESKKFPLAFPEDYHGKDVAGKT
CCCCCEECCCCCCCCEEEECCCCCCCCHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCCC
AEFTVTLKQVEWAHLPEVNEAFAQSLGIADGNLDKMRADIRENLEREVKRRTHALLKDQV
EEEEEEEECCCHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEALLKVAELDVPKSLVEQDQERLVEMARRDLESRGMPNAKNMPIPAEMFTQQAERRVKL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
GLVLAEVVKANGLEAKPEQIKAEIEEFAKSYEDPKEVIRWYYGDQQRLAEMEAYVLENNV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCC
VNFVCDKAKVTDKAVSFEELTATPAQG
EEEEECCHHHHHHHCCHHHHCCCCCCC
>Mature Secondary Structure 
STIENLGKLDRKVTLAVPKAEVEQEKQSRLARLSKTVKMSGFRPGKVPMKMVEKQYGQQ
CCHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
VEFEVRYDKAARQFFDITNEQGVKVAGQPRFELKTEGVADDQVAFDATFEVYPEVKLGDL
EEEEEEHHHHHHHHHHCCCCCCCEECCCCCEEEECCCCCCCCEEEEEEEEECCCCCCCCC
ASAELTRTKTEITDAEIDKTLDILRKQRVHYHPRGEAGEHGDGGAAVAQDGDRVTVDFVG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEEEEE
TIDGVEFAGGKAEGFSFVLGEGRMLPEFEQATLGLKQGESKKFPLAFPEDYHGKDVAGKT
CCCCCEECCCCCCCCEEEECCCCCCCCHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCCC
AEFTVTLKQVEWAHLPEVNEAFAQSLGIADGNLDKMRADIRENLEREVKRRTHALLKDQV
EEEEEEEECCCHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEALLKVAELDVPKSLVEQDQERLVEMARRDLESRGMPNAKNMPIPAEMFTQQAERRVKL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
GLVLAEVVKANGLEAKPEQIKAEIEEFAKSYEDPKEVIRWYYGDQQRLAEMEAYVLENNV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCC
VNFVCDKAKVTDKAVSFEELTATPAQG
EEEEECCHHHHHHHCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]