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The map label for this gene is mnmC [H]

Identifier: 187928952

GI number: 187928952

Start: 1971912

End: 1973930

Strand: Reverse

Name: mnmC [H]

Synonym: Rpic_1871

Alternate gene names: 187928952

Gene position: 1973930-1971912 (Counterclockwise)

Preceding gene: 187928953

Following gene: 187928951

Centisome position: 50.07

GC content: 67.11

Gene sequence:

>2019_bases
ATGCCACGCGGCCTCGTTCTCGCCACGCCTCAGCTCACGTCGGACGGCACGCCGTTCTCCGCGCAATACGACGACGTCTA
TCACAGCTCCGAAGGCGGTCTGGCCCAGGCGATTCACGTTTTCCTGGGCGGTAACCGCTTGCCGGGCGCGTGGCAAGGCA
AACGCACCTTCACCATTGTCGAAACCGGCTTCGGCCAGGGCTTGAATTTCCTGGCCACCTGGGCTGCATGGCGCGACGAT
CCACAGCGTTGTGCACGGCTCGATTTCGTCTCGATCGAGAAGCATCCGTTCGATCGCGCAGGCTTGTCGATCGTGCATCC
GACAATCGGTGCGCTGGCTCCGCTGGCCGAGACGCTCCGTCAATCATGGCCGGTGCCGGTGCCCGGCGTGCATCGACTGG
CGTTCGACGGCGGCCGCGTTACGCTGATGCTGTTTTTCGGTGATGCGATGGAGATGCTGCCGCAACTGTCGTGCGCTGCC
GACGCGTTCTATCTCGACGGTTTTTCTCCCGCCAAGAACCCCGATCTGTGGCAGCCGCGCGTGTTCAGGCAGCTCGCGCG
GGTCGCTCGCGCCGGCGCGACATTGGCCACGTACACCGCCGCAGGATTCGTGCGGCGCGGCTTGCAGGAGGCCGGCTTCG
AGGCGCACAAGGCGCACGGTTTCGGAACCAAGCGCGACATGACCGTCGCGCAATTTCCGACCCATTGGCGCACACGGCGT
GGGCCGGCTGAAGCGCCTGTCTGGAGCCAGCGTCACGCCATCGTGCTTGGCGCCGGGCTCGCGGGCTGCTCGGTTGCGGA
ACGGCTCGCGTCGCGCGGCTGGCGCATCACGCTCATCGACGCAAACGATGGCCCCGCACACGGCACGTCGGCGCACCGTG
CTGCGGCCATGCATCCGCATGTATCCATCGACGACAGCGTGCTCTCGCGCCTATCGCGCGCCGGCAACCTGCTTGCACGC
CGCCATTGGGATGCACTCGATCGCGCCGGCTTCGCCACAGGTTTCCAGCCCACCGGCGTGCTCCAACTCGCCGAACACGT
GGGCGACGCCACTGAACAGCAACGCATCGTGCGGGCGCTCGGCTACCCGGTCGACTACATCGATTGGCTCAATGTGGACG
CCGCCGCCAAGCGCGTCGGTGCCAGCGTGCCGCAGGGCGGCTGGTGGTTTGCGCAAGCGGGCTGGGTTGCGCCGCCTGAT
GTTTGCCGTGCCAATCTGGCCGCTGCAGGCGCTGCAATCGACACACGTTGGGGTACGCACGTCGAATCGCTGCGCCAAGA
CGGCGATCAATGGCAGGTCCTCGATGCGCAGGGCGATGTGATCGCGAGCGCGCCGGTCGTCATCCTCGCCAACGGTCTTG
GCGCTACGCGCCTGGCTGCGCTGACATCCGCGTCGCTCAAGCCCGTTCGCGGTCAACTCACCGACGTGCCCATCACCAGT
TTGGAAGCGGGCGCCGCGTGGCCGAAGGCCGTCGTCTGCGGCGATGGCTACTTGCTGCCCACCGAGCCGGGCGCGAAAAC
GGTGCGCACGGGCTCGTCGTTCCAGCCCGGTGAAGCCGATCTGACCGAGCGCGTGGCCGATCACGCGGGCAATCTCCGGC
GACTGTCTGGGTTGCAGCCTCAGCAATTGGGTGCACTGGCGAAGCTGGACCCTGCGCAACTGCAGGGCTACGTCGGTGTG
CGGTGCGTGTCAGCCAATCGGCTGCCGTTGATCGGCCCGCTGGTGGATGAGGCGGCGGCCTTGGCGCCGGGCTTTCGTTT
GCGCGGTCCGCATGCGCAGCTGCCTCGCCTCGCCGGGCTGTATGCCGCACTTGCGTATGGCTCGCGGGGCCTCACCTGGT
CGGTACTGGGTGCGGAGTTGCTGGCGGCCCAGATCGACGGTGGTCCGCTGCCGCTTGAATCGGACCTGGCTGCAGCGCTT
GATCCCGGCCGCTTCATCCTGCGCGCATTGCAGCTTCGACGCACGCAGGAGCAGGGCGCCGCACCCGCGGAAAGCGAAGC
TGCGGAACCTCTGGATTAA

Upstream 100 bases:

>100_bases
CGGTCCGGCATCCAGCCTGAAGACGGCAAGCGGACTGCTTCAGATCAAAGCGCAGGGGAAGGGCGGCGTTACAATTCGCT
GCTTCTTCCCAGGGTGTTCC

Downstream 100 bases:

>100_bases
CGCGCCGTTTGAAGGGTTTTCCAAGCGTTCCGACCCAAAAAAACAGGGCCGGCGAACGTTCCATGCCATAATATGCGTTT
TGCGTTTGCCAGCCTGAACG

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 672; Mature: 671

Protein sequence:

>672_residues
MPRGLVLATPQLTSDGTPFSAQYDDVYHSSEGGLAQAIHVFLGGNRLPGAWQGKRTFTIVETGFGQGLNFLATWAAWRDD
PQRCARLDFVSIEKHPFDRAGLSIVHPTIGALAPLAETLRQSWPVPVPGVHRLAFDGGRVTLMLFFGDAMEMLPQLSCAA
DAFYLDGFSPAKNPDLWQPRVFRQLARVARAGATLATYTAAGFVRRGLQEAGFEAHKAHGFGTKRDMTVAQFPTHWRTRR
GPAEAPVWSQRHAIVLGAGLAGCSVAERLASRGWRITLIDANDGPAHGTSAHRAAAMHPHVSIDDSVLSRLSRAGNLLAR
RHWDALDRAGFATGFQPTGVLQLAEHVGDATEQQRIVRALGYPVDYIDWLNVDAAAKRVGASVPQGGWWFAQAGWVAPPD
VCRANLAAAGAAIDTRWGTHVESLRQDGDQWQVLDAQGDVIASAPVVILANGLGATRLAALTSASLKPVRGQLTDVPITS
LEAGAAWPKAVVCGDGYLLPTEPGAKTVRTGSSFQPGEADLTERVADHAGNLRRLSGLQPQQLGALAKLDPAQLQGYVGV
RCVSANRLPLIGPLVDEAAALAPGFRLRGPHAQLPRLAGLYAALAYGSRGLTWSVLGAELLAAQIDGGPLPLESDLAAAL
DPGRFILRALQLRRTQEQGAAPAESEAAEPLD

Sequences:

>Translated_672_residues
MPRGLVLATPQLTSDGTPFSAQYDDVYHSSEGGLAQAIHVFLGGNRLPGAWQGKRTFTIVETGFGQGLNFLATWAAWRDD
PQRCARLDFVSIEKHPFDRAGLSIVHPTIGALAPLAETLRQSWPVPVPGVHRLAFDGGRVTLMLFFGDAMEMLPQLSCAA
DAFYLDGFSPAKNPDLWQPRVFRQLARVARAGATLATYTAAGFVRRGLQEAGFEAHKAHGFGTKRDMTVAQFPTHWRTRR
GPAEAPVWSQRHAIVLGAGLAGCSVAERLASRGWRITLIDANDGPAHGTSAHRAAAMHPHVSIDDSVLSRLSRAGNLLAR
RHWDALDRAGFATGFQPTGVLQLAEHVGDATEQQRIVRALGYPVDYIDWLNVDAAAKRVGASVPQGGWWFAQAGWVAPPD
VCRANLAAAGAAIDTRWGTHVESLRQDGDQWQVLDAQGDVIASAPVVILANGLGATRLAALTSASLKPVRGQLTDVPITS
LEAGAAWPKAVVCGDGYLLPTEPGAKTVRTGSSFQPGEADLTERVADHAGNLRRLSGLQPQQLGALAKLDPAQLQGYVGV
RCVSANRLPLIGPLVDEAAALAPGFRLRGPHAQLPRLAGLYAALAYGSRGLTWSVLGAELLAAQIDGGPLPLESDLAAAL
DPGRFILRALQLRRTQEQGAAPAESEAAEPLD
>Mature_671_residues
PRGLVLATPQLTSDGTPFSAQYDDVYHSSEGGLAQAIHVFLGGNRLPGAWQGKRTFTIVETGFGQGLNFLATWAAWRDDP
QRCARLDFVSIEKHPFDRAGLSIVHPTIGALAPLAETLRQSWPVPVPGVHRLAFDGGRVTLMLFFGDAMEMLPQLSCAAD
AFYLDGFSPAKNPDLWQPRVFRQLARVARAGATLATYTAAGFVRRGLQEAGFEAHKAHGFGTKRDMTVAQFPTHWRTRRG
PAEAPVWSQRHAIVLGAGLAGCSVAERLASRGWRITLIDANDGPAHGTSAHRAAAMHPHVSIDDSVLSRLSRAGNLLARR
HWDALDRAGFATGFQPTGVLQLAEHVGDATEQQRIVRALGYPVDYIDWLNVDAAAKRVGASVPQGGWWFAQAGWVAPPDV
CRANLAAAGAAIDTRWGTHVESLRQDGDQWQVLDAQGDVIASAPVVILANGLGATRLAALTSASLKPVRGQLTDVPITSL
EAGAAWPKAVVCGDGYLLPTEPGAKTVRTGSSFQPGEADLTERVADHAGNLRRLSGLQPQQLGALAKLDPAQLQGYVGVR
CVSANRLPLIGPLVDEAAALAPGFRLRGPHAQLPRLAGLYAALAYGSRGLTWSVLGAELLAAQIDGGPLPLESDLAAALD
PGRFILRALQLRRTQEQGAAPAESEAAEPLD

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=665, Percent_Identity=36.5413533834586, Blast_Score=370, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 71542; Mature: 71411

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRGLVLATPQLTSDGTPFSAQYDDVYHSSEGGLAQAIHVFLGGNRLPGAWQGKRTFTIV
CCCCEEEECCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEE
ETGFGQGLNFLATWAAWRDDPQRCARLDFVSIEKHPFDRAGLSIVHPTIGALAPLAETLR
ECCCCCCHHHHHHHHHHCCCHHHHHHCCEEEECCCCCCCCCCEEECHHHHHHHHHHHHHH
QSWPVPVPGVHRLAFDGGRVTLMLFFGDAMEMLPQLSCAADAFYLDGFSPAKNPDLWQPR
HCCCCCCCCCEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHH
VFRQLARVARAGATLATYTAAGFVRRGLQEAGFEAHKAHGFGTKRDMTVAQFPTHWRTRR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHCCCHHHCCC
GPAEAPVWSQRHAIVLGAGLAGCSVAERLASRGWRITLIDANDGPAHGTSAHRAAAMHPH
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHCCCC
VSIDDSVLSRLSRAGNLLARRHWDALDRAGFATGFQPTGVLQLAEHVGDATEQQRIVRAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHH
GYPVDYIDWLNVDAAAKRVGASVPQGGWWFAQAGWVAPPDVCRANLAAAGAAIDTRWGTH
CCCCCHHHHCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCHHCCCCCHH
VESLRQDGDQWQVLDAQGDVIASAPVVILANGLGATRLAALTSASLKPVRGQLTDVPITS
HHHHHCCCCCEEEEECCCCEEECCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
LEAGAAWPKAVVCGDGYLLPTEPGAKTVRTGSSFQPGEADLTERVADHAGNLRRLSGLQP
CCCCCCCCCEEEECCCEEECCCCCCCCEECCCCCCCCCHHHHHHHHHHCCCHHHHCCCCH
QQLGALAKLDPAQLQGYVGVRCVSANRLPLIGPLVDEAAALAPGFRLRGPHAQLPRLAGL
HHHCHHHCCCHHHHCCCEEEEEECCCCCCEECHHHHHHHHHCCCCEECCCCHHHHHHHHH
YAALAYGSRGLTWSVLGAELLAAQIDGGPLPLESDLAAALDPGRFILRALQLRRTQEQGA
HHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCC
APAESEAAEPLD
CCCCCCCCCCCC
>Mature Secondary Structure 
PRGLVLATPQLTSDGTPFSAQYDDVYHSSEGGLAQAIHVFLGGNRLPGAWQGKRTFTIV
CCCEEEECCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEE
ETGFGQGLNFLATWAAWRDDPQRCARLDFVSIEKHPFDRAGLSIVHPTIGALAPLAETLR
ECCCCCCHHHHHHHHHHCCCHHHHHHCCEEEECCCCCCCCCCEEECHHHHHHHHHHHHHH
QSWPVPVPGVHRLAFDGGRVTLMLFFGDAMEMLPQLSCAADAFYLDGFSPAKNPDLWQPR
HCCCCCCCCCEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHH
VFRQLARVARAGATLATYTAAGFVRRGLQEAGFEAHKAHGFGTKRDMTVAQFPTHWRTRR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHCCCHHHCCC
GPAEAPVWSQRHAIVLGAGLAGCSVAERLASRGWRITLIDANDGPAHGTSAHRAAAMHPH
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHCCCC
VSIDDSVLSRLSRAGNLLARRHWDALDRAGFATGFQPTGVLQLAEHVGDATEQQRIVRAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHH
GYPVDYIDWLNVDAAAKRVGASVPQGGWWFAQAGWVAPPDVCRANLAAAGAAIDTRWGTH
CCCCCHHHHCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCHHCCCCCHH
VESLRQDGDQWQVLDAQGDVIASAPVVILANGLGATRLAALTSASLKPVRGQLTDVPITS
HHHHHCCCCCEEEEECCCCEEECCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
LEAGAAWPKAVVCGDGYLLPTEPGAKTVRTGSSFQPGEADLTERVADHAGNLRRLSGLQP
CCCCCCCCCEEEECCCEEECCCCCCCCEECCCCCCCCCHHHHHHHHHHCCCHHHHCCCCH
QQLGALAKLDPAQLQGYVGVRCVSANRLPLIGPLVDEAAALAPGFRLRGPHAQLPRLAGL
HHHCHHHCCCHHHHCCCEEEEEECCCCCCEECHHHHHHHHHCCCCEECCCCHHHHHHHHH
YAALAYGSRGLTWSVLGAELLAAQIDGGPLPLESDLAAALDPGRFILRALQLRRTQEQGA
HHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCC
APAESEAAEPLD
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]