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The map label for this gene is oprM [H]

Identifier: 187928753

GI number: 187928753

Start: 1755400

End: 1756917

Strand: Reverse

Name: oprM [H]

Synonym: Rpic_1670

Alternate gene names: 187928753

Gene position: 1756917-1755400 (Counterclockwise)

Preceding gene: 187928756

Following gene: 187928751

Centisome position: 44.56

GC content: 63.04

Gene sequence:

>1518_bases
ATGCCCGCCAACACTGCCCACGGCATGCCATCGTCCGCCACTGTTCTTCTGCGCCTGACCATCGTGGCGGCGGCATGCCT
GTTGACCGCCGCCTGCGCGGTCGGACCGGATTTCAAGACTCCGGATGCTCCGAAAGTGACTGGCTATACGCCCAAGCCGC
TGCCGCAGCAGACATCGGCTGCAGCCACGCAGGGCGGCGAAGCACAGCGCTTTGTCTCGGGGATGCCAGTTTCCGATCAA
TGGTGGAAGGCGTTCCAGTCAGAAAAGCTGAACAAGACGATTGCCGATGCGATTTCCGCCAGCCCCACGCTGGCCGCCGC
CCAGGCTGCACTGCGCGTGGCGCAGCAGCAAACGCGCGCGCAACAAGGAGCGTTCTTCCCAATGCTGCAAGGCTCGTATC
AACCGAGCCGACAGCAAAACGCCGTCGGCACGATTTCGCCCACGCTCACCTCTGGCGCACCGATCTACACCCTGCACACC
GCGCAGCTCACGGTGAGCTATGCCCCAGACGTCTTCGGCCTGAACCGCCGGCAGGTGGAATCACTGAAGGCCACCGAAGA
GGCACAGTACTTCCAGATGGAGGCGGCTCACCTCACCCTTGCGTCCAACATCGTGGTGGCGGCAGTGCAGGAAGCCTCGC
TGCGCGCGCAGATTGAAGCGCAACAACGCATTGTCGGCATCAATACGCAGTTGCTCGACAACCTGCACAAGCAGTTCGCG
CTGGGCGCGGTAACGGGGCTGGATATTGCAGCTGCCGAAACGGCGCTTGCGCAGGCCGAGCAGGCGCTACCCAACCTGCA
AAAGCAACTGGCGGTTCAGCGCGATCTGTTGGCTGCACTGGCCGGCAAGCTGCCTAGCGAAGGCATCGCCGAGACGTTCA
CCTTTGCAGACTTCACGCTGCCGCAGGACTTGCCCGTGTCGCTGCCATCGGATCTCGTCCGGCAGCGCCCTGACGTGCGC
GCAGCCGAAGAACAGCTGCACGCCGCAACAGCGCAGGTGGGCGTGGCGATCGCCAATATGCTGCCCCAACTGACGATCAC
CGGCACCAAGGGCGGCACCGCAGAAATCTTCAGCCAGATGTTCCGGGACGGCAACGTCTTCTGGAGCCTGGTCGGCAATG
TGGCGCAGACCTTCTTCGATGGTGGTGCGATGCTGGCGAAAAAACGGGCAGCCGATGCGGGGGTCGATCAAGCGGAGGCG
CAGTACCGTGGCACCGTCATCACTGCATTCCAGAACGTCGCCGATACCCTGCACGCGCTAGATGCCGATGCCGACGTGCT
GAAAGCCGCGCTCAGATCCGAACAGGCAGCACACCGAACTCTAAACATCACGGATAGGCAGCGCGCGATCGGCCAGGTCA
ACATCCTCGCTGTGCTGAATGCAGAACAGGCCTACCAGACTGCTGTGCAAGCCACGATTGGGGCGCGTGCAAACCGATTC
GCCGATACGGCAGCGCTGTTTCAAGCCCTAGGTGGCGGGTGGTGGAACCGGCCCAACGCTGACGTCGCACGGCGCTAA

Upstream 100 bases:

>100_bases
TGCTGCCCGCACGTGGAGCGGTCTACTGTGATGAATCACAGTTCGCAACGATTCGACGCCCCCATAGAATCGGCGCCATT
CGACCAAACTGGGTGCTTCC

Downstream 100 bases:

>100_bases
AACCGATGAGGCGCCAGCCTTCTCGCTGAGGGCTGACGGTTAACTCAATGGGACTTCAAAGATCCATTTGAACGTCCACC
GGTCGGTGGCGCGCGTTAAG

Product: NodT family RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MPANTAHGMPSSATVLLRLTIVAAACLLTAACAVGPDFKTPDAPKVTGYTPKPLPQQTSAAATQGGEAQRFVSGMPVSDQ
WWKAFQSEKLNKTIADAISASPTLAAAQAALRVAQQQTRAQQGAFFPMLQGSYQPSRQQNAVGTISPTLTSGAPIYTLHT
AQLTVSYAPDVFGLNRRQVESLKATEEAQYFQMEAAHLTLASNIVVAAVQEASLRAQIEAQQRIVGINTQLLDNLHKQFA
LGAVTGLDIAAAETALAQAEQALPNLQKQLAVQRDLLAALAGKLPSEGIAETFTFADFTLPQDLPVSLPSDLVRQRPDVR
AAEEQLHAATAQVGVAIANMLPQLTITGTKGGTAEIFSQMFRDGNVFWSLVGNVAQTFFDGGAMLAKKRAADAGVDQAEA
QYRGTVITAFQNVADTLHALDADADVLKAALRSEQAAHRTLNITDRQRAIGQVNILAVLNAEQAYQTAVQATIGARANRF
ADTAALFQALGGGWWNRPNADVARR

Sequences:

>Translated_505_residues
MPANTAHGMPSSATVLLRLTIVAAACLLTAACAVGPDFKTPDAPKVTGYTPKPLPQQTSAAATQGGEAQRFVSGMPVSDQ
WWKAFQSEKLNKTIADAISASPTLAAAQAALRVAQQQTRAQQGAFFPMLQGSYQPSRQQNAVGTISPTLTSGAPIYTLHT
AQLTVSYAPDVFGLNRRQVESLKATEEAQYFQMEAAHLTLASNIVVAAVQEASLRAQIEAQQRIVGINTQLLDNLHKQFA
LGAVTGLDIAAAETALAQAEQALPNLQKQLAVQRDLLAALAGKLPSEGIAETFTFADFTLPQDLPVSLPSDLVRQRPDVR
AAEEQLHAATAQVGVAIANMLPQLTITGTKGGTAEIFSQMFRDGNVFWSLVGNVAQTFFDGGAMLAKKRAADAGVDQAEA
QYRGTVITAFQNVADTLHALDADADVLKAALRSEQAAHRTLNITDRQRAIGQVNILAVLNAEQAYQTAVQATIGARANRF
ADTAALFQALGGGWWNRPNADVARR
>Mature_504_residues
PANTAHGMPSSATVLLRLTIVAAACLLTAACAVGPDFKTPDAPKVTGYTPKPLPQQTSAAATQGGEAQRFVSGMPVSDQW
WKAFQSEKLNKTIADAISASPTLAAAQAALRVAQQQTRAQQGAFFPMLQGSYQPSRQQNAVGTISPTLTSGAPIYTLHTA
QLTVSYAPDVFGLNRRQVESLKATEEAQYFQMEAAHLTLASNIVVAAVQEASLRAQIEAQQRIVGINTQLLDNLHKQFAL
GAVTGLDIAAAETALAQAEQALPNLQKQLAVQRDLLAALAGKLPSEGIAETFTFADFTLPQDLPVSLPSDLVRQRPDVRA
AEEQLHAATAQVGVAIANMLPQLTITGTKGGTAEIFSQMFRDGNVFWSLVGNVAQTFFDGGAMLAKKRAADAGVDQAEAQ
YRGTVITAFQNVADTLHALDADADVLKAALRSEQAAHRTLNITDRQRAIGQVNILAVLNAEQAYQTAVQATIGARANRFA
DTAALFQALGGGWWNRPNADVARR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=484, Percent_Identity=26.8595041322314, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI2367348, Length=367, Percent_Identity=24.2506811989101, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53588; Mature: 53457

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPANTAHGMPSSATVLLRLTIVAAACLLTAACAVGPDFKTPDAPKVTGYTPKPLPQQTSA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
AATQGGEAQRFVSGMPVSDQWWKAFQSEKLNKTIADAISASPTLAAAQAALRVAQQQTRA
HHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QQGAFFPMLQGSYQPSRQQNAVGTISPTLTSGAPIYTLHTAQLTVSYAPDVFGLNRRQVE
HCCCCCHHHCCCCCCCHHCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCHHHHH
SLKATEEAQYFQMEAAHLTLASNIVVAAVQEASLRAQIEAQQRIVGINTQLLDNLHKQFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LGAVTGLDIAAAETALAQAEQALPNLQKQLAVQRDLLAALAGKLPSEGIAETFTFADFTL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCC
PQDLPVSLPSDLVRQRPDVRAAEEQLHAATAQVGVAIANMLPQLTITGTKGGTAEIFSQM
CCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHH
FRDGNVFWSLVGNVAQTFFDGGAMLAKKRAADAGVDQAEAQYRGTVITAFQNVADTLHAL
HHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHH
DADADVLKAALRSEQAAHRTLNITDRQRAIGQVNILAVLNAEQAYQTAVQATIGARANRF
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCEEEEEEECHHHHHHHHHHHHHHHHHHHH
ADTAALFQALGGGWWNRPNADVARR
HHHHHHHHHHCCCCCCCCCCHHCCC
>Mature Secondary Structure 
PANTAHGMPSSATVLLRLTIVAAACLLTAACAVGPDFKTPDAPKVTGYTPKPLPQQTSA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
AATQGGEAQRFVSGMPVSDQWWKAFQSEKLNKTIADAISASPTLAAAQAALRVAQQQTRA
HHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QQGAFFPMLQGSYQPSRQQNAVGTISPTLTSGAPIYTLHTAQLTVSYAPDVFGLNRRQVE
HCCCCCHHHCCCCCCCHHCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCHHHHH
SLKATEEAQYFQMEAAHLTLASNIVVAAVQEASLRAQIEAQQRIVGINTQLLDNLHKQFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LGAVTGLDIAAAETALAQAEQALPNLQKQLAVQRDLLAALAGKLPSEGIAETFTFADFTL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCC
PQDLPVSLPSDLVRQRPDVRAAEEQLHAATAQVGVAIANMLPQLTITGTKGGTAEIFSQM
CCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHH
FRDGNVFWSLVGNVAQTFFDGGAMLAKKRAADAGVDQAEAQYRGTVITAFQNVADTLHAL
HHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHH
DADADVLKAALRSEQAAHRTLNITDRQRAIGQVNILAVLNAEQAYQTAVQATIGARANRF
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCEEEEEEECHHHHHHHHHHHHHHHHHHHH
ADTAALFQALGGGWWNRPNADVARR
HHHHHHHHHHCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]