Click here to switch to the map view.

The map label for this gene is hipO [H]

Identifier: 187928496

GI number: 187928496

Start: 1500306

End: 1501520

Strand: Direct

Name: hipO [H]

Synonym: Rpic_1410

Alternate gene names: 187928496

Gene position: 1500306-1501520 (Clockwise)

Preceding gene: 187928495

Following gene: 187928497

Centisome position: 38.05

GC content: 64.53

Gene sequence:

>1215_bases
ATGACGAAAGCCCCCACGCTCACGCCGTTCCAACTGTTGCCGCATCTCCTGCCGCCCGTCCATATCGATGCAGAAACGTT
CGTCAACATCCGCCGTCAGATCCACGCGCAGCCCGAACTCGGTTTCGAGGTGGGCGCCACCAGCACGTTGGTGGCCGATC
TGCTCAAGAGTTGGGGTTACGAGGTGCACACGGGCATCGGCAAGAGCGGCGTGGTGGGCCAACTGAAGCTGGGCAACGGC
ACGCGCCGCCTTGGCATTCGCGCGGACATGGACGCCCTGCCCATTGTTGAAGCGACCGGCCTGCCTTACGCCAGCCGCAA
CCACGGCAAGATGCATGCGTGCGGCCACGACGGGCACACCGCCATCCTGCTCGCCGCAGCCAAGGCGCTGGCCGAGAGCC
GCGATTTTGATGGCACGCTGAACCTCATCTTCCAGCCCGATGAAGAAAACCTGTGCGGTGCGCGCGCCATGATCGAAGAC
GGCCTCTTCGAGCGCTTCCCCTGCGATGCCGTCTATGCCTTGCACAACATGCCGGGCGTGCCGGCCGGCAGCTTCCGCGT
GCTGCCGGGACCGGTGAGCCTGTCCTCCGACGTGGCCGACGTCACCATCAAGGGCATGGGCGGACACGGTGCGATGCCGC
ATCGCGCGCGTGATCCGATTGCCGCGTCGGCCGCCATCATCACCGCGTTGCAAACCGTGGTCGCACGCAATGTCGCGCCT
GACGACACGGCGGTGGTGTCGGTCGGTTTCATTCGCGGCGGTGCAACGCACAACGTGATTCCCGAATCGGTGACGATTGG
CCTGAATGTGCGCGCAGCGCGGCCGGAAACGCGTGCGCTGGTGGAAATGCGCATTCGCGAGATTGTCAGCCTGACCGCAC
AGGCGCATGGCGTCGAGGCGCAGATCGACTATCGTCAATTGACACCGCCCATGGTCAATACCGAGGCCGAGACGCAACTG
GCGCAGCAGGTCTGTACTGAACTGGTGGGTGCGGCCAACGTCGTCACCCAGGCGCCCAAGGGGCTCAACGGCAGCGAAGA
CTTTGCCTGGATGCTCAATGAAGTGCCGGGCTGCTACTTGATCCTGGGTAATGGCGAAGGCGAATTCGGCGGCTGCATGG
TGCACAACCCCGGCTACGATTTCAACGACCAGGTGTTGCCGTTGGGCGCAGCCGCCTGGGTGCGTCTGGCGCAGGCCTAT
CTGACCGCCGCGTGA

Upstream 100 bases:

>100_bases
TGGCGTGCAGCGCCATCACGCTGCTGACCGTGCTGAGCCTGCGCGAAGCGCGCGAAACTGATTGACCCCGGATTCCCATC
CCCAACCCCGGAGCCCCGAC

Downstream 100 bases:

>100_bases
AGTGAACTTCGAGTCAAGCAATGCAAGCGAATCCGAGCCACGCTGCAACACCTGCGGCCGCCAACGAAGAGATCGTCCAA
TGGAGCGCGATCGAACTGTC

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MTKAPTLTPFQLLPHLLPPVHIDAETFVNIRRQIHAQPELGFEVGATSTLVADLLKSWGYEVHTGIGKSGVVGQLKLGNG
TRRLGIRADMDALPIVEATGLPYASRNHGKMHACGHDGHTAILLAAAKALAESRDFDGTLNLIFQPDEENLCGARAMIED
GLFERFPCDAVYALHNMPGVPAGSFRVLPGPVSLSSDVADVTIKGMGGHGAMPHRARDPIAASAAIITALQTVVARNVAP
DDTAVVSVGFIRGGATHNVIPESVTIGLNVRAARPETRALVEMRIREIVSLTAQAHGVEAQIDYRQLTPPMVNTEAETQL
AQQVCTELVGAANVVTQAPKGLNGSEDFAWMLNEVPGCYLILGNGEGEFGGCMVHNPGYDFNDQVLPLGAAAWVRLAQAY
LTAA

Sequences:

>Translated_404_residues
MTKAPTLTPFQLLPHLLPPVHIDAETFVNIRRQIHAQPELGFEVGATSTLVADLLKSWGYEVHTGIGKSGVVGQLKLGNG
TRRLGIRADMDALPIVEATGLPYASRNHGKMHACGHDGHTAILLAAAKALAESRDFDGTLNLIFQPDEENLCGARAMIED
GLFERFPCDAVYALHNMPGVPAGSFRVLPGPVSLSSDVADVTIKGMGGHGAMPHRARDPIAASAAIITALQTVVARNVAP
DDTAVVSVGFIRGGATHNVIPESVTIGLNVRAARPETRALVEMRIREIVSLTAQAHGVEAQIDYRQLTPPMVNTEAETQL
AQQVCTELVGAANVVTQAPKGLNGSEDFAWMLNEVPGCYLILGNGEGEFGGCMVHNPGYDFNDQVLPLGAAAWVRLAQAY
LTAA
>Mature_403_residues
TKAPTLTPFQLLPHLLPPVHIDAETFVNIRRQIHAQPELGFEVGATSTLVADLLKSWGYEVHTGIGKSGVVGQLKLGNGT
RRLGIRADMDALPIVEATGLPYASRNHGKMHACGHDGHTAILLAAAKALAESRDFDGTLNLIFQPDEENLCGARAMIEDG
LFERFPCDAVYALHNMPGVPAGSFRVLPGPVSLSSDVADVTIKGMGGHGAMPHRARDPIAASAAIITALQTVVARNVAPD
DTAVVSVGFIRGGATHNVIPESVTIGLNVRAARPETRALVEMRIREIVSLTAQAHGVEAQIDYRQLTPPMVNTEAETQLA
QQVCTELVGAANVVTQAPKGLNGSEDFAWMLNEVPGCYLILGNGEGEFGGCMVHNPGYDFNDQVLPLGAAAWVRLAQAYL
TAA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=421, Percent_Identity=27.5534441805226, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 42847; Mature: 42715

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAPTLTPFQLLPHLLPPVHIDAETFVNIRRQIHAQPELGFEVGATSTLVADLLKSWGY
CCCCCCCCHHHHHHHHCCCCEECHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHCCC
EVHTGIGKSGVVGQLKLGNGTRRLGIRADMDALPIVEATGLPYASRNHGKMHACGHDGHT
EEECCCCCCCCEEEEEECCCCEEECEEECCCCCCEEEECCCCCCCCCCCCEEECCCCCCH
AILLAAAKALAESRDFDGTLNLIFQPDEENLCGARAMIEDGLFERFPCDAVYALHNMPGV
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHCCCHHHHHHHCCCCC
PAGSFRVLPGPVSLSSDVADVTIKGMGGHGAMPHRARDPIAASAAIITALQTVVARNVAP
CCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
DDTAVVSVGFIRGGATHNVIPESVTIGLNVRAARPETRALVEMRIREIVSLTAQAHGVEA
CCCEEEEEEEEECCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEE
QIDYRQLTPPMVNTEAETQLAQQVCTELVGAANVVTQAPKGLNGSEDFAWMLNEVPGCYL
EECHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEE
ILGNGEGEFGGCMVHNPGYDFNDQVLPLGAAAWVRLAQAYLTAA
EEECCCCCCCCEEEECCCCCCCCCEEECHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKAPTLTPFQLLPHLLPPVHIDAETFVNIRRQIHAQPELGFEVGATSTLVADLLKSWGY
CCCCCCCHHHHHHHHCCCCEECHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHCCC
EVHTGIGKSGVVGQLKLGNGTRRLGIRADMDALPIVEATGLPYASRNHGKMHACGHDGHT
EEECCCCCCCCEEEEEECCCCEEECEEECCCCCCEEEECCCCCCCCCCCCEEECCCCCCH
AILLAAAKALAESRDFDGTLNLIFQPDEENLCGARAMIEDGLFERFPCDAVYALHNMPGV
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHCCCHHHHHHHCCCCC
PAGSFRVLPGPVSLSSDVADVTIKGMGGHGAMPHRARDPIAASAAIITALQTVVARNVAP
CCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
DDTAVVSVGFIRGGATHNVIPESVTIGLNVRAARPETRALVEMRIREIVSLTAQAHGVEA
CCCEEEEEEEEECCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEE
QIDYRQLTPPMVNTEAETQLAQQVCTELVGAANVVTQAPKGLNGSEDFAWMLNEVPGCYL
EECHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEE
ILGNGEGEFGGCMVHNPGYDFNDQVLPLGAAAWVRLAQAYLTAA
EEECCCCCCCCEEEECCCCCCCCCEEECHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]