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The map label for this gene is citH [H]

Identifier: 187928495

GI number: 187928495

Start: 1498966

End: 1500270

Strand: Direct

Name: citH [H]

Synonym: Rpic_1409

Alternate gene names: 187928495

Gene position: 1498966-1500270 (Clockwise)

Preceding gene: 187928493

Following gene: 187928496

Centisome position: 38.02

GC content: 66.28

Gene sequence:

>1305_bases
ATGAACGACGCCACCTTGCAGACTACGGCCCTGCCCGCCTCGGCCGTTGCTGCGCCCAAGCCCGCCGGCCAGGCGCGATT
GATCGCCGCGTGCTCGATCGGCAATGCGCTGGAAATGTACGACTTCACGGTCTACAGCTTCTTCGCGCTGATGATCGGCA
AGCTGTTTTTTCCCTCCGACAGCGCGTACGGCTCGTTGCTGCTCGCGGTGGCGACGTTCGGCATCGGCTTTGTGATGCGG
CCGCTGGGCGGTTTCGTCATCGGCAACTACGCAGACCGCCACGGACGCAAGGCCGCGATGACGCTGACAATCGGGCTGAT
GGTCATCGGCACGCTGTGCCTGGCGGTTGCGCCCACATACGCCACCGCAGGCCTGCTCGGCCCGCTGGTGATCCTTGCCG
GGCGGTTGCTGCAGGGCTTCTCGCTGGGCGGTGAAATCGGCGCGTCGACGGCGATGCTGATGGAGTCCGGCGGTATCCGC
GGGCGCGGTTTTCGCGTGAGCTGGCAGTTGGGCAGCCAGGGCATTGCAGCGCTGTGCGGCGCGCTGACTGCGGCCATTCT
GTACGGCAGCCTGTCGCCGGAAGCGCTGCAGAGCTGGGGCTGGCGTGTGCCGTTCTTCCTTGGCCTGCTGATTGCGCCGG
TGGGCCTGTATATCCGCTCGCACCTGGATGAAACGCACACCGCCGAGGCGCATGCGCCGAGCCCGCTCGGCACCTTGTTT
CGCGACCACGGCGGGCTGGTCATCAAGGGCGTGCTCACGATCATCGGCGGGACAGTCGGCACGTATCTGGTCATCTACTT
CATGCCGACCTACATGATCCGCGAGCTGCATCTGCCGGCTTCGCTGTCGCTGCTGGCCGGTTGCGTGACGGGGCTGACGA
GCTTCGGCGCGTCGCTCCTTTCCGGTTGGCTGGCCGACCGCCTGCCGCGCCGCAAGCCTCTGGTGGTGGGCGCAACGCTG
GTCACCGTGGCGGCGCTCTATCCGGCCTTCTGGCTGATGACGCATTACCCGAGCGTGCCGCTGGTGCTGGCACTGTCGGC
GTTGCTCACCGGCACGCTGTACCTGGGCACCACGCCCATGCTGCTCATGATGATGGAGCTGCTGCCCATCAAGGTGCGCG
CCAGCGGCCTGTCGGTCATCTACGCGATCGGCGTCACGGTGTTTGGCGGCTCGTGCCAGTTTGTCGTGACGTGGCTGCTG
GCCCGCACCGGCAATCCGCTCTCCCCTGCCTTTTACATGATGGCGTGCAGCGCCATCACGCTGCTGACCGTGCTGAGCCT
GCGCGAAGCGCGCGAAACTGATTGA

Upstream 100 bases:

>100_bases
GAGATGAATATTTGCGATTTTTTCAGCGCCCGCGTTCCCTGAATACTGCAAGCACCCAAGGCTCAGAGAAGCCCTTTCCA
ACATCGTGGACGGAGACCCC

Downstream 100 bases:

>100_bases
CCCCGGATTCCCATCCCCAACCCCGGAGCCCCGACATGACGAAAGCCCCCACGCTCACGCCGTTCCAACTGTTGCCGCAT
CTCCTGCCGCCCGTCCATAT

Product: major facilitator superfamily protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MNDATLQTTALPASAVAAPKPAGQARLIAACSIGNALEMYDFTVYSFFALMIGKLFFPSDSAYGSLLLAVATFGIGFVMR
PLGGFVIGNYADRHGRKAAMTLTIGLMVIGTLCLAVAPTYATAGLLGPLVILAGRLLQGFSLGGEIGASTAMLMESGGIR
GRGFRVSWQLGSQGIAALCGALTAAILYGSLSPEALQSWGWRVPFFLGLLIAPVGLYIRSHLDETHTAEAHAPSPLGTLF
RDHGGLVIKGVLTIIGGTVGTYLVIYFMPTYMIRELHLPASLSLLAGCVTGLTSFGASLLSGWLADRLPRRKPLVVGATL
VTVAALYPAFWLMTHYPSVPLVLALSALLTGTLYLGTTPMLLMMMELLPIKVRASGLSVIYAIGVTVFGGSCQFVVTWLL
ARTGNPLSPAFYMMACSAITLLTVLSLREARETD

Sequences:

>Translated_434_residues
MNDATLQTTALPASAVAAPKPAGQARLIAACSIGNALEMYDFTVYSFFALMIGKLFFPSDSAYGSLLLAVATFGIGFVMR
PLGGFVIGNYADRHGRKAAMTLTIGLMVIGTLCLAVAPTYATAGLLGPLVILAGRLLQGFSLGGEIGASTAMLMESGGIR
GRGFRVSWQLGSQGIAALCGALTAAILYGSLSPEALQSWGWRVPFFLGLLIAPVGLYIRSHLDETHTAEAHAPSPLGTLF
RDHGGLVIKGVLTIIGGTVGTYLVIYFMPTYMIRELHLPASLSLLAGCVTGLTSFGASLLSGWLADRLPRRKPLVVGATL
VTVAALYPAFWLMTHYPSVPLVLALSALLTGTLYLGTTPMLLMMMELLPIKVRASGLSVIYAIGVTVFGGSCQFVVTWLL
ARTGNPLSPAFYMMACSAITLLTVLSLREARETD
>Mature_434_residues
MNDATLQTTALPASAVAAPKPAGQARLIAACSIGNALEMYDFTVYSFFALMIGKLFFPSDSAYGSLLLAVATFGIGFVMR
PLGGFVIGNYADRHGRKAAMTLTIGLMVIGTLCLAVAPTYATAGLLGPLVILAGRLLQGFSLGGEIGASTAMLMESGGIR
GRGFRVSWQLGSQGIAALCGALTAAILYGSLSPEALQSWGWRVPFFLGLLIAPVGLYIRSHLDETHTAEAHAPSPLGTLF
RDHGGLVIKGVLTIIGGTVGTYLVIYFMPTYMIRELHLPASLSLLAGCVTGLTSFGASLLSGWLADRLPRRKPLVVGATL
VTVAALYPAFWLMTHYPSVPLVLALSALLTGTLYLGTTPMLLMMMELLPIKVRASGLSVIYAIGVTVFGGSCQFVVTWLL
ARTGNPLSPAFYMMACSAITLLTVLSLREARETD

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=421, Percent_Identity=30.8788598574822, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1788942, Length=403, Percent_Identity=32.5062034739454, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1788292, Length=414, Percent_Identity=28.743961352657, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1789941, Length=428, Percent_Identity=31.0747663551402, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI87082231, Length=222, Percent_Identity=30.1801801801802, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45691; Mature: 45691

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDATLQTTALPASAVAAPKPAGQARLIAACSIGNALEMYDFTVYSFFALMIGKLFFPSD
CCCCCEEECCCCHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCC
SAYGSLLLAVATFGIGFVMRPLGGFVIGNYADRHGRKAAMTLTIGLMVIGTLCLAVAPTY
CHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ATAGLLGPLVILAGRLLQGFSLGGEIGASTAMLMESGGIRGRGFRVSWQLGSQGIAALCG
HHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHH
ALTAAILYGSLSPEALQSWGWRVPFFLGLLIAPVGLYIRSHLDETHTAEAHAPSPLGTLF
HHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHH
RDHGGLVIKGVLTIIGGTVGTYLVIYFMPTYMIRELHLPASLSLLAGCVTGLTSFGASLL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SGWLADRLPRRKPLVVGATLVTVAALYPAFWLMTHYPSVPLVLALSALLTGTLYLGTTPM
HHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHH
LLMMMELLPIKVRASGLSVIYAIGVTVFGGSCQFVVTWLLARTGNPLSPAFYMMACSAIT
HHHHHHHHCHHEECCCHHHHEEHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LLTVLSLREARETD
HHHHHHHHHHCCCC
>Mature Secondary Structure
MNDATLQTTALPASAVAAPKPAGQARLIAACSIGNALEMYDFTVYSFFALMIGKLFFPSD
CCCCCEEECCCCHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCC
SAYGSLLLAVATFGIGFVMRPLGGFVIGNYADRHGRKAAMTLTIGLMVIGTLCLAVAPTY
CHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ATAGLLGPLVILAGRLLQGFSLGGEIGASTAMLMESGGIRGRGFRVSWQLGSQGIAALCG
HHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHH
ALTAAILYGSLSPEALQSWGWRVPFFLGLLIAPVGLYIRSHLDETHTAEAHAPSPLGTLF
HHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHH
RDHGGLVIKGVLTIIGGTVGTYLVIYFMPTYMIRELHLPASLSLLAGCVTGLTSFGASLL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SGWLADRLPRRKPLVVGATLVTVAALYPAFWLMTHYPSVPLVLALSALLTGTLYLGTTPM
HHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHH
LLMMMELLPIKVRASGLSVIYAIGVTVFGGSCQFVVTWLLARTGNPLSPAFYMMACSAIT
HHHHHHHHCHHEECCCHHHHEEHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LLTVLSLREARETD
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]