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The map label for this gene is glnG [H]

Identifier: 187928182

GI number: 187928182

Start: 1160591

End: 1162099

Strand: Direct

Name: glnG [H]

Synonym: Rpic_1089

Alternate gene names: 187928182

Gene position: 1160591-1162099 (Clockwise)

Preceding gene: 187928181

Following gene: 187928185

Centisome position: 29.44

GC content: 64.74

Gene sequence:

>1509_bases
ATGAAGCCAATCTGGATAGTCGACGACGATCAATCCATCCGCTGGGTCCTTGAGAAGGCGCTGGCGCGTGAGAGCCTGCT
CTCGCGCAGCTTTACCAACGTGCGCGATGCGCTCAACGCGCTCGACGAAGAAACACCGCAAGTCCTGATCTCGGACGTCC
GTATGCCGGGCGGCTCGGGGCTGGATCTGCTGCAGGCCATCAAGGCCAAACACCCCGGACTGCCGGTCATCATCATGACC
GCATATTCGGACCTGGACAGCGCCGTGGCGGCGTTCCAGGGCGGCGCGTTTGAATACCTGGCCAAGCCGTTCGACGTGGA
TCGTGCGGTCGAACTGATCCGCCGCGCGCTGGAAGAGAGCCTGCGCGAAGAGGAGATCGACGATCGCCTGAGCGACGCGC
CTGAGATTCTCGGGCAGGCGCCGGCCATGCAGGACGTGTTCCGCGCGATCGGGCGGCTGTCGCAGTCCAACGTGACGGTG
CTGATCACCGGCGAATCCGGCACGGGTAAGGAGCTGGTGGCACGGGCGCTGCATAAGCACAGCCCGCGTGCGAGCGGCCC
CTTTATCGCACTGAACACCGCGGCGATTCCGAAAGACTTGCTGGAATCCGAGCTGTTTGGCCATGAGCGCGGCGCCTTTA
CTGGCGCGCAGACCATGCGCCGCGGCCGCTTTGAGCAAGCCGAGGGCGGCACGCTGTTCCTCGATGAAATCGGCGACATG
CCGTTCGATCTGCAGACGCGCTTACTGCGCGTGCTGTCGGACGGCCACTTCTATCGCGTGGGCGGCCACAACCCGCTCAA
GGCCAACGTGCGCGTCATCGCGGCCACGCACCAGAATCTTGAAACCCGCGTCAAGGACGGTCTGTTCCGCGAGGACTTGT
TTCACCGCCTGAACGTGATCCGTCTGCGCTTGCCGGCGCTGCGCGAACGCCCGGAAGACATCACGCTGCTGGCGCGCCAT
TTCTTGCAGAAGAGTGCCAAGGAGCTGGGCGTGGAGCCCAAGCGCATGTCGGACGATGCGCTGGCGCATATCGCCACGCT
GCCGTTCCCCGGCAACGTGCGGCAACTGGAAAACCTCTGCAACTGGCTCACTGTGATGGCGCCGGCGCAGACCATTGAAA
GCAAGGATCTGCCCGCCGACCTGGTGCGAGGCAACGCGCAGATGCTACCGGTCATGGGCGAGGCCGCGGCTCGCCCAGCC
TTCATCGGCGAGCCGTCGCATGGTGGCGAACCGTATGGTGTTACGCCGTCGGTCGATACGTTGCTCTCAAGCTCTGCAGC
GGGGGCATCCATCGCAGCAGGTTGGGAGCGCGCGCTCGCGGGCGAGGCCAAGGCCATGCTCGAAGCGGGCCAGCCCGACG
TCATGGACGCACTTACGCGACGCTTCGAGAAGGCGATTCTTGAGGCTGCGCTGGGTGTCACGCGCGGGCGCCGTGTTGAA
GCGGCAACGCGTCTGGGCATCGGCCGCAACACCATCACGCGCAAATTGCAGGAGCTTGGCTTCGACTGA

Upstream 100 bases:

>100_bases
GATTTCCGCATTCTCGTGCCGCTGCACTGAAGCGCCTCAGCTTCAATAACGCAGCGCGAGGCGGCCCAGGCGAGACGAAG
TCAAGCAGACGAACCAACAC

Downstream 100 bases:

>100_bases
CGTGCCGTCAGCGGGGCCAGTCCACTGGATATCGTCGAGCTTGGCGATTGGGATTGGGCGGATTTCCGATAAGGGAATCC
GCCCTTTTTTTGGCGGCCGA

Product: nitrogen metabolism transcriptional regulator, NtrC, Fis Family

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MKPIWIVDDDQSIRWVLEKALARESLLSRSFTNVRDALNALDEETPQVLISDVRMPGGSGLDLLQAIKAKHPGLPVIIMT
AYSDLDSAVAAFQGGAFEYLAKPFDVDRAVELIRRALEESLREEEIDDRLSDAPEILGQAPAMQDVFRAIGRLSQSNVTV
LITGESGTGKELVARALHKHSPRASGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTLFLDEIGDM
PFDLQTRLLRVLSDGHFYRVGGHNPLKANVRVIAATHQNLETRVKDGLFREDLFHRLNVIRLRLPALRERPEDITLLARH
FLQKSAKELGVEPKRMSDDALAHIATLPFPGNVRQLENLCNWLTVMAPAQTIESKDLPADLVRGNAQMLPVMGEAAARPA
FIGEPSHGGEPYGVTPSVDTLLSSSAAGASIAAGWERALAGEAKAMLEAGQPDVMDALTRRFEKAILEAALGVTRGRRVE
AATRLGIGRNTITRKLQELGFD

Sequences:

>Translated_502_residues
MKPIWIVDDDQSIRWVLEKALARESLLSRSFTNVRDALNALDEETPQVLISDVRMPGGSGLDLLQAIKAKHPGLPVIIMT
AYSDLDSAVAAFQGGAFEYLAKPFDVDRAVELIRRALEESLREEEIDDRLSDAPEILGQAPAMQDVFRAIGRLSQSNVTV
LITGESGTGKELVARALHKHSPRASGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTLFLDEIGDM
PFDLQTRLLRVLSDGHFYRVGGHNPLKANVRVIAATHQNLETRVKDGLFREDLFHRLNVIRLRLPALRERPEDITLLARH
FLQKSAKELGVEPKRMSDDALAHIATLPFPGNVRQLENLCNWLTVMAPAQTIESKDLPADLVRGNAQMLPVMGEAAARPA
FIGEPSHGGEPYGVTPSVDTLLSSSAAGASIAAGWERALAGEAKAMLEAGQPDVMDALTRRFEKAILEAALGVTRGRRVE
AATRLGIGRNTITRKLQELGFD
>Mature_502_residues
MKPIWIVDDDQSIRWVLEKALARESLLSRSFTNVRDALNALDEETPQVLISDVRMPGGSGLDLLQAIKAKHPGLPVIIMT
AYSDLDSAVAAFQGGAFEYLAKPFDVDRAVELIRRALEESLREEEIDDRLSDAPEILGQAPAMQDVFRAIGRLSQSNVTV
LITGESGTGKELVARALHKHSPRASGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTLFLDEIGDM
PFDLQTRLLRVLSDGHFYRVGGHNPLKANVRVIAATHQNLETRVKDGLFREDLFHRLNVIRLRLPALRERPEDITLLARH
FLQKSAKELGVEPKRMSDDALAHIATLPFPGNVRQLENLCNWLTVMAPAQTIESKDLPADLVRGNAQMLPVMGEAAARPA
FIGEPSHGGEPYGVTPSVDTLLSSSAAGASIAAGWERALAGEAKAMLEAGQPDVMDALTRRFEKAILEAALGVTRGRRVE
AATRLGIGRNTITRKLQELGFD

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=502, Percent_Identity=56.3745019920319, Blast_Score=543, Evalue=1e-156,
Organism=Escherichia coli, GI1788550, Length=505, Percent_Identity=40.5940594059406, Blast_Score=354, Evalue=8e-99,
Organism=Escherichia coli, GI1790437, Length=383, Percent_Identity=43.3420365535248, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1788905, Length=392, Percent_Identity=43.6224489795918, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI87082117, Length=392, Percent_Identity=39.7959183673469, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1789087, Length=249, Percent_Identity=48.5943775100402, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1789233, Length=258, Percent_Identity=46.1240310077519, Blast_Score=222, Evalue=5e-59,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=47.6595744680851, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1786524, Length=248, Percent_Identity=48.3870967741936, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1787583, Length=367, Percent_Identity=36.2397820163488, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI87081872, Length=226, Percent_Identity=46.4601769911504, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI87081858, Length=448, Percent_Identity=27.2321428571429, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789828, Length=163, Percent_Identity=40.4907975460123, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1790860, Length=117, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54912; Mature: 54912

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4 ; PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIWIVDDDQSIRWVLEKALARESLLSRSFTNVRDALNALDEETPQVLISDVRMPGGSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
LDLLQAIKAKHPGLPVIIMTAYSDLDSAVAAFQGGAFEYLAKPFDVDRAVELIRRALEES
HHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHH
LREEEIDDRLSDAPEILGQAPAMQDVFRAIGRLSQSNVTVLITGESGTGKELVARALHKH
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHC
SPRASGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTLFLDEIGDM
CCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEHHHHCCC
PFDLQTRLLRVLSDGHFYRVGGHNPLKANVRVIAATHQNLETRVKDGLFREDLFHRLNVI
CHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECHHHHHHHHHHCHHHHHHHHHHHHH
RLRLPALRERPEDITLLARHFLQKSAKELGVEPKRMSDDALAHIATLPFPGNVRQLENLC
HHCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHH
NWLTVMAPAQTIESKDLPADLVRGNAQMLPVMGEAAARPAFIGEPSHGGEPYGVTPSVDT
HHHHHHCCHHHHCCCCCCHHHHCCCCCEECCHHHHHCCCCCCCCCCCCCCCCCCCCCHHH
LLSSSAAGASIAAGWERALAGEAKAMLEAGQPDVMDALTRRFEKAILEAALGVTRGRRVE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AATRLGIGRNTITRKLQELGFD
HHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPIWIVDDDQSIRWVLEKALARESLLSRSFTNVRDALNALDEETPQVLISDVRMPGGSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
LDLLQAIKAKHPGLPVIIMTAYSDLDSAVAAFQGGAFEYLAKPFDVDRAVELIRRALEES
HHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHH
LREEEIDDRLSDAPEILGQAPAMQDVFRAIGRLSQSNVTVLITGESGTGKELVARALHKH
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHC
SPRASGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTLFLDEIGDM
CCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEHHHHCCC
PFDLQTRLLRVLSDGHFYRVGGHNPLKANVRVIAATHQNLETRVKDGLFREDLFHRLNVI
CHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECHHHHHHHHHHCHHHHHHHHHHHHH
RLRLPALRERPEDITLLARHFLQKSAKELGVEPKRMSDDALAHIATLPFPGNVRQLENLC
HHCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHH
NWLTVMAPAQTIESKDLPADLVRGNAQMLPVMGEAAARPAFIGEPSHGGEPYGVTPSVDT
HHHHHHCCHHHHCCCCCCHHHHCCCCCEECCHHHHHCCCCCCCCCCCCCCCCCCCCCHHH
LLSSSAAGASIAAGWERALAGEAKAMLEAGQPDVMDALTRRFEKAILEAALGVTRGRRVE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AATRLGIGRNTITRKLQELGFD
HHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]