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The map label for this gene is citH [H]

Identifier: 187927643

GI number: 187927643

Start: 582045

End: 583340

Strand: Direct

Name: citH [H]

Synonym: Rpic_0543

Alternate gene names: 187927643

Gene position: 582045-583340 (Clockwise)

Preceding gene: 187927642

Following gene: 187927644

Centisome position: 14.76

GC content: 63.19

Gene sequence:

>1296_bases
ATGCACCCCCCGTCCAGCCTTTCGACCGGGCAATCCAAGGCCGCTGCGGTCTTCCGCGTCACCGCCGGGAATTTCCTGGA
GCAGTTCGATTTCTTCCTGTTCGGCTTTTACGCCACGCAGATTGCCAACGTCTTCTTTCCGACCGAAAGCGAATTCGCCT
CGCTGATGATGACGTTCGCTGTTTTTGGGGCTGGGTTCCTCATGCGCCCGCTGGGCGCAGTCGTGCTCGGCGCCTACATT
GACGATGTCGGTCGCCGAAAGGGCCTGATCGTCACGCTGTCGATCATGGCAAGCGGGACCGTGCTGATTGCGTTTGTTCC
CGGCTACGCCACGATCGGCCTGCTGGCACCGGCGCTCGTGTTGCTGGGGCGTCTATTGCAGGGTTTCTCGGCGGGCGCGG
AACTGGGCGGCGTATCGGTGTATCTGGCCGAGATGGCCACGCCCGGCCGCAAGGGTTTTTTCACGAGCTGGCAGTCCGCC
AGCCAGCAGGTCGCAATCGTCGTAGCGGCGGGCCTGGGCTTCGCGCTCAACCAAGGGCTGAGCGCACCGGCCATCGCCGC
ATGGGGATGGCGTGTGCCGTTTTTCGTCGGTTGCATGATCGTGCCGTTCATCTTCGTGCTGCGCCGTAATCTTGAAGAAA
CGCAGGAGTTCAAGGCACGCAAGCATCGTCCGAGCATGAAGGACGTACTCAGCACGCTGGTGCAGAACTGGGGCGTCGTC
ATCGCCGGCATGATGCTGGTGGCGATGACGACCACGAGCTTCTACCTCATCACGGTGTATGCGCCCACGTTCGGGAAGAC
GGTCCTGCACCTCAGCACGGCCGACAGCCTGCTCGTCACGCTCTGCGTGGGGGTGTCGAACTTCGTGTGGCTGCCGATTG
GCGGTGCGCTCTCCGACAGGATTGGCCGTCGGCCACTGCTCATCGGCATGACGGTGCTGACCATCGCCACTGCGTATCCG
GCGCTTTCGTTTCTCGCGCACGCGCCGAGCTTCATGAATATGCTGCTCGTGCTGCTCTGGCTGTCGTTCATGTACGGCAT
CTACAACGGCGCGATGATCGTTGCGCTCACGGAGGTGATGCCCGTGCAGGTGCGCGTTGCAGGGTTCTCGCTCGCCTACA
GCTTGGCGACGGCGGTATTCGGCGGCTTCACCCCCGCCATCTCGACAGCGGCCATTCATATGACCGGCGACAAGGCGGCC
CCTGGCTACTGGATGAGTCTTGCCGCCGTGTGCGCTCTCCTGGCGACGTTGACGCTCTATCGCCGCCGCGCGGTGACGTT
GGCTCCGGCGCACTGA

Upstream 100 bases:

>100_bases
AATTGCACTTCTCGTTTGTCGGTCTCCCGGCGAACATTGCCAAAACGACAAGCGCCGTCCAACGGCGTATTCTTTTTTTT
GGCTTCCAGGAGACACCTCG

Downstream 100 bases:

>100_bases
CGCGCAGCACGTTCCGTATATTCACAAGCAACGCACGACCTGGCACGACCCTCACCATGAAGACTCTGTTCCTGAAACTC
TGCGCAGCCGCGCTCGTCGC

Product: major facilitator superfamily metabolite/H+ symporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MHPPSSLSTGQSKAAAVFRVTAGNFLEQFDFFLFGFYATQIANVFFPTESEFASLMMTFAVFGAGFLMRPLGAVVLGAYI
DDVGRRKGLIVTLSIMASGTVLIAFVPGYATIGLLAPALVLLGRLLQGFSAGAELGGVSVYLAEMATPGRKGFFTSWQSA
SQQVAIVVAAGLGFALNQGLSAPAIAAWGWRVPFFVGCMIVPFIFVLRRNLEETQEFKARKHRPSMKDVLSTLVQNWGVV
IAGMMLVAMTTTSFYLITVYAPTFGKTVLHLSTADSLLVTLCVGVSNFVWLPIGGALSDRIGRRPLLIGMTVLTIATAYP
ALSFLAHAPSFMNMLLVLLWLSFMYGIYNGAMIVALTEVMPVQVRVAGFSLAYSLATAVFGGFTPAISTAAIHMTGDKAA
PGYWMSLAAVCALLATLTLYRRRAVTLAPAH

Sequences:

>Translated_431_residues
MHPPSSLSTGQSKAAAVFRVTAGNFLEQFDFFLFGFYATQIANVFFPTESEFASLMMTFAVFGAGFLMRPLGAVVLGAYI
DDVGRRKGLIVTLSIMASGTVLIAFVPGYATIGLLAPALVLLGRLLQGFSAGAELGGVSVYLAEMATPGRKGFFTSWQSA
SQQVAIVVAAGLGFALNQGLSAPAIAAWGWRVPFFVGCMIVPFIFVLRRNLEETQEFKARKHRPSMKDVLSTLVQNWGVV
IAGMMLVAMTTTSFYLITVYAPTFGKTVLHLSTADSLLVTLCVGVSNFVWLPIGGALSDRIGRRPLLIGMTVLTIATAYP
ALSFLAHAPSFMNMLLVLLWLSFMYGIYNGAMIVALTEVMPVQVRVAGFSLAYSLATAVFGGFTPAISTAAIHMTGDKAA
PGYWMSLAAVCALLATLTLYRRRAVTLAPAH
>Mature_431_residues
MHPPSSLSTGQSKAAAVFRVTAGNFLEQFDFFLFGFYATQIANVFFPTESEFASLMMTFAVFGAGFLMRPLGAVVLGAYI
DDVGRRKGLIVTLSIMASGTVLIAFVPGYATIGLLAPALVLLGRLLQGFSAGAELGGVSVYLAEMATPGRKGFFTSWQSA
SQQVAIVVAAGLGFALNQGLSAPAIAAWGWRVPFFVGCMIVPFIFVLRRNLEETQEFKARKHRPSMKDVLSTLVQNWGVV
IAGMMLVAMTTTSFYLITVYAPTFGKTVLHLSTADSLLVTLCVGVSNFVWLPIGGALSDRIGRRPLLIGMTVLTIATAYP
ALSFLAHAPSFMNMLLVLLWLSFMYGIYNGAMIVALTEVMPVQVRVAGFSLAYSLATAVFGGFTPAISTAAIHMTGDKAA
PGYWMSLAAVCALLATLTLYRRRAVTLAPAH

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=426, Percent_Identity=33.5680751173709, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1790550, Length=416, Percent_Identity=28.8461538461538, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1788292, Length=392, Percent_Identity=29.3367346938776, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1789941, Length=412, Percent_Identity=29.8543689320388, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI87082231, Length=200, Percent_Identity=29, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46042; Mature: 46042

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPPSSLSTGQSKAAAVFRVTAGNFLEQFDFFLFGFYATQIANVFFPTESEFASLMMTFA
CCCCCCCCCCCCHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VFGAGFLMRPLGAVVLGAYIDDVGRRKGLIVTLSIMASGTVLIAFVPGYATIGLLAPALV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEECCHHHHHHHHHHHH
LLGRLLQGFSAGAELGGVSVYLAEMATPGRKGFFTSWQSASQQVAIVVAAGLGFALNQGL
HHHHHHHHHCCCCCCCCHHEEEEHHCCCCCCCCCCCHHCCCCEEEEEEEECCCHHHHCCC
SAPAIAAWGWRVPFFVGCMIVPFIFVLRRNLEETQEFKARKHRPSMKDVLSTLVQNWGVV
CCCCHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IAGMMLVAMTTTSFYLITVYAPTFGKTVLHLSTADSLLVTLCVGVSNFVWLPIGGALSDR
HHHHHHHHHHHCCEEEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCEEEEECCCHHHHH
IGRRPLLIGMTVLTIATAYPALSFLAHAPSFMNMLLVLLWLSFMYGIYNGAMIVALTEVM
CCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHEEEEHHHC
PVQVRVAGFSLAYSLATAVFGGFTPAISTAAIHMTGDKAAPGYWMSLAAVCALLATLTLY
EEEEEEEHHHHHHHHHHHHHCCCCHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
RRRAVTLAPAH
HHHHCEECCCC
>Mature Secondary Structure
MHPPSSLSTGQSKAAAVFRVTAGNFLEQFDFFLFGFYATQIANVFFPTESEFASLMMTFA
CCCCCCCCCCCCHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VFGAGFLMRPLGAVVLGAYIDDVGRRKGLIVTLSIMASGTVLIAFVPGYATIGLLAPALV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEECCHHHHHHHHHHHH
LLGRLLQGFSAGAELGGVSVYLAEMATPGRKGFFTSWQSASQQVAIVVAAGLGFALNQGL
HHHHHHHHHCCCCCCCCHHEEEEHHCCCCCCCCCCCHHCCCCEEEEEEEECCCHHHHCCC
SAPAIAAWGWRVPFFVGCMIVPFIFVLRRNLEETQEFKARKHRPSMKDVLSTLVQNWGVV
CCCCHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IAGMMLVAMTTTSFYLITVYAPTFGKTVLHLSTADSLLVTLCVGVSNFVWLPIGGALSDR
HHHHHHHHHHHCCEEEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCEEEEECCCHHHHH
IGRRPLLIGMTVLTIATAYPALSFLAHAPSFMNMLLVLLWLSFMYGIYNGAMIVALTEVM
CCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHEEEEHHHC
PVQVRVAGFSLAYSLATAVFGGFTPAISTAAIHMTGDKAAPGYWMSLAAVCALLATLTLY
EEEEEEEHHHHHHHHHHHHHCCCCHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
RRRAVTLAPAH
HHHHCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]