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The map label for this gene is hemK [C]

Identifier: 187918070

GI number: 187918070

Start: 193639

End: 194472

Strand: Direct

Name: hemK [C]

Synonym: BH0197

Alternate gene names: 187918070

Gene position: 193639-194472 (Clockwise)

Preceding gene: 187918069

Following gene: 187918071

Centisome position: 21.0

GC content: 29.86

Gene sequence:

>834_bases
ATGACAATAAACGAAGCAATAAAGAGTTCAAAACAACATAACTTAAATACTCTTGAGATTTTATTACTACTTGAAAAAAT
CTTAAAGAATCGAAAAGAATTAATTCTGGCAAATACAAATAAGACTTTAACAAAACAAGAAGAACATAAATTACTGAGTC
AAATAAAAAACATAAAATCAGGCACACCCATAAACTACATACTTGGAACAAAGGAATTCATGGGAATCAAGTTTTACATA
AACAAACATGTGCTAATTCCTAGAGCAGATACAGAATGCCTAGTAGAAGAAGCTTTAATTCAAATTAAAAAGCACAACTT
AAATAGAATCTTAGATTTATGTTGCGGGAGTGGGTGCATTGGATTAACAATTGCATATTATCTTAAGCGCAAAGTAACAC
TATCAGATATTTCGACTAAAGCCTTAAGGGTATCATTAAGAAACACACAAAGACTAAAATTAGAAAATTATATAGAAATA
CAACATTCGGATCTGTTAAAATACATAGATAAAGAGTTTGAGCTAATAATCACTAATCCTCCTTATTTAAATAAAGATGA
ACTAAAAATAAAGGAAAAATTAGCAAAAGAACCAAGAATAGCTCTTTTAGGATTTGGAAGAGATGGGCTTAAGATTCCAA
AAAAAATAATAATACAGGCAAAACATAAGCTCACCAAAAATGGCCTCTTAATAGTAGAAATGGCTCCATGGCAAACAAAC
TCTCTAAAAAAATTTGCAATCCAGGAAGGTTTTGAATATTTGAAAACCATATATGACATTGAAAACAGAGAAAGGGCATT
GGTCCTAAGGATAAAGCATGATACAAGTCTATGA

Upstream 100 bases:

>100_bases
AGAAGAAATTATGCAAGGAGAACTTGATTTTCTTCTCGACACACTAGCTCTAAAATTTCAAGAACAATCACTAAAAGATA
ATTCAATTTAATTTCTTCTA

Downstream 100 bases:

>100_bases
AATTGCATACCTACTTAAAATAAATGATATTGATAAACTAAAAAACATTTTTAGAAAAATTGTCAATAATACTTATCAAG
ATGAGATTCAAAAAAAATTA

Product: peptide release factor-glutamine N5-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTINEAIKSSKQHNLNTLEILLLLEKILKNRKELILANTNKTLTKQEEHKLLSQIKNIKSGTPINYILGTKEFMGIKFYI
NKHVLIPRADTECLVEEALIQIKKHNLNRILDLCCGSGCIGLTIAYYLKRKVTLSDISTKALRVSLRNTQRLKLENYIEI
QHSDLLKYIDKEFELIITNPPYLNKDELKIKEKLAKEPRIALLGFGRDGLKIPKKIIIQAKHKLTKNGLLIVEMAPWQTN
SLKKFAIQEGFEYLKTIYDIENRERALVLRIKHDTSL

Sequences:

>Translated_277_residues
MTINEAIKSSKQHNLNTLEILLLLEKILKNRKELILANTNKTLTKQEEHKLLSQIKNIKSGTPINYILGTKEFMGIKFYI
NKHVLIPRADTECLVEEALIQIKKHNLNRILDLCCGSGCIGLTIAYYLKRKVTLSDISTKALRVSLRNTQRLKLENYIEI
QHSDLLKYIDKEFELIITNPPYLNKDELKIKEKLAKEPRIALLGFGRDGLKIPKKIIIQAKHKLTKNGLLIVEMAPWQTN
SLKKFAIQEGFEYLKTIYDIENRERALVLRIKHDTSL
>Mature_276_residues
TINEAIKSSKQHNLNTLEILLLLEKILKNRKELILANTNKTLTKQEEHKLLSQIKNIKSGTPINYILGTKEFMGIKFYIN
KHVLIPRADTECLVEEALIQIKKHNLNRILDLCCGSGCIGLTIAYYLKRKVTLSDISTKALRVSLRNTQRLKLENYIEIQ
HSDLLKYIDKEFELIITNPPYLNKDELKIKEKLAKEPRIALLGFGRDGLKIPKKIIIQAKHKLTKNGLLIVEMAPWQTNS
LKKFAIQEGFEYLKTIYDIENRERALVLRIKHDTSL

Specific function: Methylates The Translation Termination Release Factor Rf1 On Gln-235 And Rf2 On Gln-252. [C]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=194, Percent_Identity=31.9587628865979, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1787463, Length=271, Percent_Identity=28.7822878228782, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI87082085, Length=198, Percent_Identity=32.8282828282828, Blast_Score=110, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24582226, Length=216, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR017127
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32039; Mature: 31908

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTINEAIKSSKQHNLNTLEILLLLEKILKNRKELILANTNKTLTKQEEHKLLSQIKNIKS
CCHHHHHHCHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCC
GTPINYILGTKEFMGIKFYINKHVLIPRADTECLVEEALIQIKKHNLNRILDLCCGSGCI
CCCEEEEECCHHHEEEEEEEECEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
GLTIAYYLKRKVTLSDISTKALRVSLRNTQRLKLENYIEIQHSDLLKYIDKEFELIITNP
HHHHHHHHHHCCCHHHCCHHHHEEECCCCCEEEHHHHEEEHHHHHHHHHCCCCEEEECCC
PYLNKDELKIKEKLAKEPRIALLGFGRDGLKIPKKIIIQAKHKLTKNGLLIVEMAPWQTN
CCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
SLKKFAIQEGFEYLKTIYDIENRERALVLRIKHDTSL
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCC
>Mature Secondary Structure 
TINEAIKSSKQHNLNTLEILLLLEKILKNRKELILANTNKTLTKQEEHKLLSQIKNIKS
CHHHHHHCHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCC
GTPINYILGTKEFMGIKFYINKHVLIPRADTECLVEEALIQIKKHNLNRILDLCCGSGCI
CCCEEEEECCHHHEEEEEEEECEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
GLTIAYYLKRKVTLSDISTKALRVSLRNTQRLKLENYIEIQHSDLLKYIDKEFELIITNP
HHHHHHHHHHCCCHHHCCHHHHEEECCCCCEEEHHHHEEEHHHHHHHHHCCCCEEEECCC
PYLNKDELKIKEKLAKEPRIALLGFGRDGLKIPKKIIIQAKHKLTKNGLLIVEMAPWQTN
CCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
SLKKFAIQEGFEYLKTIYDIENRERALVLRIKHDTSL
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]