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The map label for this gene is spoT [H]

Identifier: 187918071

GI number: 187918071

Start: 194456

End: 196459

Strand: Direct

Name: spoT [H]

Synonym: BH0198

Alternate gene names: 187918071

Gene position: 194456-196459 (Clockwise)

Preceding gene: 187918070

Following gene: 187918072

Centisome position: 21.08

GC content: 28.74

Gene sequence:

>2004_bases
ATGATACAAGTCTATGAAATTGCATACCTACTTAAAATAAATGATATTGATAAACTAAAAAACATTTTTAGAAAAATTGT
CAATAATACTTATCAAGATGAGATTCAAAAAAAATTAATATTTAAAGCTCTTGAAATATCAGAACAATTACATTACGGAC
AATATAGAGAAAGTAAAGAGCCCTACATAATCCATCCAATAATGGTTGCATTATTTCTCATAAAATTTCAACTAGACTTT
AAAACAATAATAGCTGGCCTATTGCACGACGTTCTTGAAGATACGAGTGTGAAAAAGGAAGAAATAATTAAAGAATTTGA
TCAAGAAGTTTTAAGTCTAATTGATGGTGTAACCAAAATTCATGACTTACACAATAAAACAAGAGCAATCAAAGAAGCAA
ATACCATTTCAAAAATGTTTTTTGCAATGACCCATGATATTAGAATAATAATCATAAAACTTGCAGATAAATTACATAAT
ATGGCAACCCTTTCGCACTTACCTAAAAACAGAAGGGAAAGAATTGCAAGAGACTGTCTTGCCACTTACGTACCAATTGC
AGAAAGACTTGGTATTTCATCTCTTAAAATATATCTTGAAGATTTATCATTAAAATATCTTTATCCAAAAGAATATAAAG
AAATCAAAAATTTTCTATCCGCAACAAAAATAGAGAGAGAAAAAAAATTATATAAGGGAAAATTGATCATAGAGAAAGAA
CTTAAAAAAATTGGAATCGATGTCGTAATCACAGTACGCTCTAAACATTTCTACTCAATATTTAGAAAAATGAAAACAAG
AAATAATAATATTGCTCAAATATTTGATACCCTGGGAATAAGAATAATTTGTAAAAAACAAAAAGAATGCTACGAAATAC
TAGAAATCGTACATAAGGTTTGGAAACCAATACCTGGTAGATTAAAAGACTACATAGCAATCCCTAAAGAGAATAAATAT
CAATCCCTACATACTACCGTCAGAATACCTGAAGACAACCAATTAATTGAAATACAAATTAGAACAGAAGAAATGGACAG
AATTGCTAAATATGGTGTTGCTGCTCACTGGCTTTACAAAGAACAAGTTGAATTAAAAGCAGATGATATATCATTTATCA
ACCGAATCAAAAAATGGCAACAAGAATCAGTTAACAAAAATCAATATTCAATGTACGACATACACAAAGAACTCTTAAAC
ACATTTATATATGTATATACACCGGAAGGAGAAATAGTAGAACTCCCATTTGGCTCAAACTCAATTGACTTTGCGTACAC
AATACATACAGATATTGGGGATCAAGCACTTTACGCAAAAATCAATGGCAAAATTAGCTCACTAACTAAACCATTGAAAA
ATGAACAAATTGTTGAAATATTTACCTCTCCAGAAGCAAAACCTGATGTAATTTGGCTAAATAGCGTTAGAACAAAAAAA
GCACGCTCAAAAATTCGATCTTGGCTTAATAAAAATGATAATACAATATTTGTAGATAATAATATAATTGCATACCTTAT
TGGGGAGAATAAGGAACAAAAAAGACTCTTCAGTCTGTTTAAATCTTTAACAAAAGACAAACTAAAAAGTATTACAATAG
CCTCTGACTGTAATCCATTAACAGATGAAGACATCATCGGAATAATACAAAAAGACACAATAGTAGTTCACAAAGAACAT
TGTAAAGAAATAATACATCAGAAAAAAAGCCATCTTGTAGAAGTAGAATGGGAAGCAACACCAACAAGGAAAGTATATCA
TATTATAATATTTTTAAAGAATTTAAAAGGTCTTTTTAATTATTTAGATAACCTTTTTACAATCTTTGACGTAAGACTTA
TTAGTGAAAAAATAGAAGATTGCGGGAATGGACATGGAATAATCAACATAATCATATCATCAAATGCCAAAAACGTATCA
ATGATTCTTACTTCCCTTAAAGAAAATCCCAATGTACTCCAAATAATGCAAGTAGAAGAAGATATAAAAAATTATGATAA
TTAA

Upstream 100 bases:

>100_bases
AACTCTCTAAAAAAATTTGCAATCCAGGAAGGTTTTGAATATTTGAAAACCATATATGACATTGAAAACAGAGAAAGGGC
ATTGGTCCTAAGGATAAAGC

Downstream 100 bases:

>100_bases
GATATTACTCTTAATCTTAATACACGCATTAATTCCACATAAAATAATAAAAGCTGACGAGAGAGAACAATGTAGCAAAC
AAATAAGAAAACATATTACA

Product: guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 667; Mature: 667

Protein sequence:

>667_residues
MIQVYEIAYLLKINDIDKLKNIFRKIVNNTYQDEIQKKLIFKALEISEQLHYGQYRESKEPYIIHPIMVALFLIKFQLDF
KTIIAGLLHDVLEDTSVKKEEIIKEFDQEVLSLIDGVTKIHDLHNKTRAIKEANTISKMFFAMTHDIRIIIIKLADKLHN
MATLSHLPKNRRERIARDCLATYVPIAERLGISSLKIYLEDLSLKYLYPKEYKEIKNFLSATKIEREKKLYKGKLIIEKE
LKKIGIDVVITVRSKHFYSIFRKMKTRNNNIAQIFDTLGIRIICKKQKECYEILEIVHKVWKPIPGRLKDYIAIPKENKY
QSLHTTVRIPEDNQLIEIQIRTEEMDRIAKYGVAAHWLYKEQVELKADDISFINRIKKWQQESVNKNQYSMYDIHKELLN
TFIYVYTPEGEIVELPFGSNSIDFAYTIHTDIGDQALYAKINGKISSLTKPLKNEQIVEIFTSPEAKPDVIWLNSVRTKK
ARSKIRSWLNKNDNTIFVDNNIIAYLIGENKEQKRLFSLFKSLTKDKLKSITIASDCNPLTDEDIIGIIQKDTIVVHKEH
CKEIIHQKKSHLVEVEWEATPTRKVYHIIIFLKNLKGLFNYLDNLFTIFDVRLISEKIEDCGNGHGIINIIISSNAKNVS
MILTSLKENPNVLQIMQVEEDIKNYDN

Sequences:

>Translated_667_residues
MIQVYEIAYLLKINDIDKLKNIFRKIVNNTYQDEIQKKLIFKALEISEQLHYGQYRESKEPYIIHPIMVALFLIKFQLDF
KTIIAGLLHDVLEDTSVKKEEIIKEFDQEVLSLIDGVTKIHDLHNKTRAIKEANTISKMFFAMTHDIRIIIIKLADKLHN
MATLSHLPKNRRERIARDCLATYVPIAERLGISSLKIYLEDLSLKYLYPKEYKEIKNFLSATKIEREKKLYKGKLIIEKE
LKKIGIDVVITVRSKHFYSIFRKMKTRNNNIAQIFDTLGIRIICKKQKECYEILEIVHKVWKPIPGRLKDYIAIPKENKY
QSLHTTVRIPEDNQLIEIQIRTEEMDRIAKYGVAAHWLYKEQVELKADDISFINRIKKWQQESVNKNQYSMYDIHKELLN
TFIYVYTPEGEIVELPFGSNSIDFAYTIHTDIGDQALYAKINGKISSLTKPLKNEQIVEIFTSPEAKPDVIWLNSVRTKK
ARSKIRSWLNKNDNTIFVDNNIIAYLIGENKEQKRLFSLFKSLTKDKLKSITIASDCNPLTDEDIIGIIQKDTIVVHKEH
CKEIIHQKKSHLVEVEWEATPTRKVYHIIIFLKNLKGLFNYLDNLFTIFDVRLISEKIEDCGNGHGIINIIISSNAKNVS
MILTSLKENPNVLQIMQVEEDIKNYDN
>Mature_667_residues
MIQVYEIAYLLKINDIDKLKNIFRKIVNNTYQDEIQKKLIFKALEISEQLHYGQYRESKEPYIIHPIMVALFLIKFQLDF
KTIIAGLLHDVLEDTSVKKEEIIKEFDQEVLSLIDGVTKIHDLHNKTRAIKEANTISKMFFAMTHDIRIIIIKLADKLHN
MATLSHLPKNRRERIARDCLATYVPIAERLGISSLKIYLEDLSLKYLYPKEYKEIKNFLSATKIEREKKLYKGKLIIEKE
LKKIGIDVVITVRSKHFYSIFRKMKTRNNNIAQIFDTLGIRIICKKQKECYEILEIVHKVWKPIPGRLKDYIAIPKENKY
QSLHTTVRIPEDNQLIEIQIRTEEMDRIAKYGVAAHWLYKEQVELKADDISFINRIKKWQQESVNKNQYSMYDIHKELLN
TFIYVYTPEGEIVELPFGSNSIDFAYTIHTDIGDQALYAKINGKISSLTKPLKNEQIVEIFTSPEAKPDVIWLNSVRTKK
ARSKIRSWLNKNDNTIFVDNNIIAYLIGENKEQKRLFSLFKSLTKDKLKSITIASDCNPLTDEDIIGIIQKDTIVVHKEH
CKEIIHQKKSHLVEVEWEATPTRKVYHIIIFLKNLKGLFNYLDNLFTIFDVRLISEKIEDCGNGHGIINIIISSNAKNVS
MILTSLKENPNVLQIMQVEEDIKNYDN

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=611, Percent_Identity=36.1702127659575, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1789147, Length=449, Percent_Identity=34.7438752783964, Blast_Score=289, Evalue=5e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 78043; Mature: 78043

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQVYEIAYLLKINDIDKLKNIFRKIVNNTYQDEIQKKLIFKALEISEQLHYGQYRESKE
CEEEEEEEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PYIIHPIMVALFLIKFQLDFKTIIAGLLHDVLEDTSVKKEEIIKEFDQEVLSLIDGVTKI
CEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
HDLHNKTRAIKEANTISKMFFAMTHDIRIIIIKLADKLHNMATLSHLPKNRRERIARDCL
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ATYVPIAERLGISSLKIYLEDLSLKYLYPKEYKEIKNFLSATKIEREKKLYKGKLIIEKE
HHHHHHHHHCCHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LKKIGIDVVITVRSKHFYSIFRKMKTRNNNIAQIFDTLGIRIICKKQKECYEILEIVHKV
HHHCCCEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHCEEEEECCHHHHHHHHHHHHHH
WKPIPGRLKDYIAIPKENKYQSLHTTVRIPEDNQLIEIQIRTEEMDRIAKYGVAAHWLYK
HCCCCCCHHHHEECCCCCCCCEEEEEEEECCCCCEEEEEEEHHHHHHHHHHCHHHHHHHH
EQVELKADDISFINRIKKWQQESVNKNQYSMYDIHKELLNTFIYVYTPEGEIVELPFGSN
HHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCCEEEECCCCC
SIDFAYTIHTDIGDQALYAKINGKISSLTKPLKNEQIVEIFTSPEAKPDVIWLNSVRTKK
CEEEEEEEECCCCCCEEEEEECCCHHHHHCCCCCCCEEEEEECCCCCCCEEEECCHHHHH
ARSKIRSWLNKNDNTIFVDNNIIAYLIGENKEQKRLFSLFKSLTKDKLKSITIASDCNPL
HHHHHHHHHCCCCCEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
TDEDIIGIIQKDTIVVHKEHCKEIIHQKKSHLVEVEWEATPTRKVYHIIIFLKNLKGLFN
CCHHHHEEEECCEEEEEHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
YLDNLFTIFDVRLISEKIEDCGNGHGIINIIISSNAKNVSMILTSLKENPNVLQIMQVEE
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEEHHH
DIKNYDN
HHCCCCC
>Mature Secondary Structure
MIQVYEIAYLLKINDIDKLKNIFRKIVNNTYQDEIQKKLIFKALEISEQLHYGQYRESKE
CEEEEEEEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PYIIHPIMVALFLIKFQLDFKTIIAGLLHDVLEDTSVKKEEIIKEFDQEVLSLIDGVTKI
CEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
HDLHNKTRAIKEANTISKMFFAMTHDIRIIIIKLADKLHNMATLSHLPKNRRERIARDCL
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ATYVPIAERLGISSLKIYLEDLSLKYLYPKEYKEIKNFLSATKIEREKKLYKGKLIIEKE
HHHHHHHHHCCHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LKKIGIDVVITVRSKHFYSIFRKMKTRNNNIAQIFDTLGIRIICKKQKECYEILEIVHKV
HHHCCCEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHCEEEEECCHHHHHHHHHHHHHH
WKPIPGRLKDYIAIPKENKYQSLHTTVRIPEDNQLIEIQIRTEEMDRIAKYGVAAHWLYK
HCCCCCCHHHHEECCCCCCCCEEEEEEEECCCCCEEEEEEEHHHHHHHHHHCHHHHHHHH
EQVELKADDISFINRIKKWQQESVNKNQYSMYDIHKELLNTFIYVYTPEGEIVELPFGSN
HHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCCEEEECCCCC
SIDFAYTIHTDIGDQALYAKINGKISSLTKPLKNEQIVEIFTSPEAKPDVIWLNSVRTKK
CEEEEEEEECCCCCCEEEEEECCCHHHHHCCCCCCCEEEEEECCCCCCCEEEECCHHHHH
ARSKIRSWLNKNDNTIFVDNNIIAYLIGENKEQKRLFSLFKSLTKDKLKSITIASDCNPL
HHHHHHHHHCCCCCEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
TDEDIIGIIQKDTIVVHKEHCKEIIHQKKSHLVEVEWEATPTRKVYHIIIFLKNLKGLFN
CCHHHHEEEECCEEEEEHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
YLDNLFTIFDVRLISEKIEDCGNGHGIINIIISSNAKNVSMILTSLKENPNVLQIMQVEE
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEEHHH
DIKNYDN
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]