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The map label for this gene is prfA

Identifier: 187918069

GI number: 187918069

Start: 192556

End: 193629

Strand: Direct

Name: prfA

Synonym: BH0196

Alternate gene names: 187918069

Gene position: 192556-193629 (Clockwise)

Preceding gene: 187918066

Following gene: 187918070

Centisome position: 20.88

GC content: 30.63

Gene sequence:

>1074_bases
ATGTTTTTAGAAAAATTAAGTCCCATTGAAAGCAAAATAAAAATACTTGAAGAAAAATTACAAGACATAAATTTAGTGAA
AAACCAAAAAGAATATGCAAAAACAGTAAAAGAATATAACTATCTAGAAAAAATTAAAGCAAAAAAAGATGAATATGAAA
ACATATTAAGCCAAATCAATGAGAATCAAAAAATTTTGTCCGAAGAAGAAAACTTAGAAATGAAAGAACTCGTAAAACAG
GAATTAGCACATTTAAACCTCAAAAAAGATGAAGTTGAACATATGATCAAAATATTACTCTTACACCAAGATGAAAATGA
CAGTAAAAATATCATTATTGAAATTAGAGCTGGAACAGGAGGAGAAGAAGCTGCACTTTTTGCACATAATCTTTATGAAA
TGTATACAAAATATTCTGAGAAAAAAAAATGGAAAACAGAGCTTATTAACTTTAATGAAACGGAACTTGGTGGATTTAAA
GAAGTAAGTTTTGAAATAAAGGGTAAAGATGTATTTAAAAAATTAAAACATGAAAGCGGGGTACATAGAGTCCAAAGAGT
TCCTATAACAGAATCCAATGGAAGACTTCAAACCTCAGCAGCAACTGTTGCTGTGCTGCCTGAAGTTGAAGATACTGACA
TTGAAATTAATGAAAAAGATTTAAGAATAGATGTTTACAGATCCTCTGGTGCTGGGGGCCAACATGTAAACACAACAGAC
TCTGCTGTTAGAATTACACATCTACCCACAGGAATCGTGGTACAATGTCAAAATGAAAGAAGTCAGCACAAAAATAAAGA
TCAGGCTATGAAAATATTAAGAGCCAGACTTTATGAATTTGAAAACCTCAAAAAACAAGAACAACGCTCAAATGACAGAA
AGCAACAAGTAGGTTCAGGTGATAGGTCTGAAAGAATTAGAACATACAATTTCCCACAAAATAGAGTAACAGACCATAGG
GCAAATATTAGTCTTTATAAACTAGAAGAAATTATGCAAGGAGAACTTGATTTTCTTCTCGACACACTAGCTCTAAAATT
TCAAGAACAATCACTAAAAGATAATTCAATTTAA

Upstream 100 bases:

>100_bases
TATTGCAAATAAAGCCTAGTAGATAAAATTCATGGGCAATTATAACAAAAAAAATCAAAAGTAGGATAAAATATTACTAC
TTTACTAGGAGAAAATTAAG

Downstream 100 bases:

>100_bases
TTTCTTCTAATGACAATAAACGAAGCAATAAAGAGTTCAAAACAACATAACTTAAATACTCTTGAGATTTTATTACTACT
TGAAAAAATCTTAAAGAATC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MFLEKLSPIESKIKILEEKLQDINLVKNQKEYAKTVKEYNYLEKIKAKKDEYENILSQINENQKILSEEENLEMKELVKQ
ELAHLNLKKDEVEHMIKILLLHQDENDSKNIIIEIRAGTGGEEAALFAHNLYEMYTKYSEKKKWKTELINFNETELGGFK
EVSFEIKGKDVFKKLKHESGVHRVQRVPITESNGRLQTSAATVAVLPEVEDTDIEINEKDLRIDVYRSSGAGGQHVNTTD
SAVRITHLPTGIVVQCQNERSQHKNKDQAMKILRARLYEFENLKKQEQRSNDRKQQVGSGDRSERIRTYNFPQNRVTDHR
ANISLYKLEEIMQGELDFLLDTLALKFQEQSLKDNSI

Sequences:

>Translated_357_residues
MFLEKLSPIESKIKILEEKLQDINLVKNQKEYAKTVKEYNYLEKIKAKKDEYENILSQINENQKILSEEENLEMKELVKQ
ELAHLNLKKDEVEHMIKILLLHQDENDSKNIIIEIRAGTGGEEAALFAHNLYEMYTKYSEKKKWKTELINFNETELGGFK
EVSFEIKGKDVFKKLKHESGVHRVQRVPITESNGRLQTSAATVAVLPEVEDTDIEINEKDLRIDVYRSSGAGGQHVNTTD
SAVRITHLPTGIVVQCQNERSQHKNKDQAMKILRARLYEFENLKKQEQRSNDRKQQVGSGDRSERIRTYNFPQNRVTDHR
ANISLYKLEEIMQGELDFLLDTLALKFQEQSLKDNSI
>Mature_357_residues
MFLEKLSPIESKIKILEEKLQDINLVKNQKEYAKTVKEYNYLEKIKAKKDEYENILSQINENQKILSEEENLEMKELVKQ
ELAHLNLKKDEVEHMIKILLLHQDENDSKNIIIEIRAGTGGEEAALFAHNLYEMYTKYSEKKKWKTELINFNETELGGFK
EVSFEIKGKDVFKKLKHESGVHRVQRVPITESNGRLQTSAATVAVLPEVEDTDIEINEKDLRIDVYRSSGAGGQHVNTTD
SAVRITHLPTGIVVQCQNERSQHKNKDQAMKILRARLYEFENLKKQEQRSNDRKQQVGSGDRSERIRTYNFPQNRVTDHR
ANISLYKLEEIMQGELDFLLDTLALKFQEQSLKDNSI

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=316, Percent_Identity=47.4683544303797, Blast_Score=295, Evalue=3e-80,
Organism=Homo sapiens, GI34577120, Length=361, Percent_Identity=37.1191135734072, Blast_Score=234, Evalue=7e-62,
Organism=Homo sapiens, GI166795305, Length=212, Percent_Identity=42.9245283018868, Blast_Score=191, Evalue=8e-49,
Organism=Escherichia coli, GI1787462, Length=352, Percent_Identity=47.1590909090909, Blast_Score=338, Evalue=3e-94,
Organism=Escherichia coli, GI2367172, Length=346, Percent_Identity=39.5953757225434, Blast_Score=226, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17542784, Length=321, Percent_Identity=37.0716510903427, Blast_Score=194, Evalue=9e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=329, Percent_Identity=42.2492401215805, Blast_Score=232, Evalue=6e-62,
Organism=Drosophila melanogaster, GI19921226, Length=336, Percent_Identity=40.1785714285714, Blast_Score=251, Evalue=4e-67,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_BORHD (B2RZQ6)

Other databases:

- EMBL:   CP000048
- RefSeq:   YP_001883632.1
- ProteinModelPortal:   B2RZQ6
- EnsemblBacteria:   EBBORT00000006777
- GeneID:   6276935
- GenomeReviews:   CP000048_GR
- KEGG:   bhr:BH0196
- GeneTree:   EBGT00050000007150
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   BHER314723:BH0196-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41548; Mature: 41548

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLEKLSPIESKIKILEEKLQDINLVKNQKEYAKTVKEYNYLEKIKAKKDEYENILSQIN
CCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ENQKILSEEENLEMKELVKQELAHLNLKKDEVEHMIKILLLHQDENDSKNIIIEIRAGTG
CCHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCC
GEEAALFAHNLYEMYTKYSEKKKWKTELINFNETELGGFKEVSFEIKGKDVFKKLKHESG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHHHHCC
VHRVQRVPITESNGRLQTSAATVAVLPEVEDTDIEINEKDLRIDVYRSSGAGGQHVNTTD
HHHHHCCCCCCCCCEEEECCEEEEEECCCCCCCCEECCCEEEEEEEECCCCCCCCCCCCC
SAVRITHLPTGIVVQCQNERSQHKNKDQAMKILRARLYEFENLKKQEQRSNDRKQQVGSG
CEEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCC
DRSERIRTYNFPQNRVTDHRANISLYKLEEIMQGELDFLLDTLALKFQEQSLKDNSI
CCCCCCEECCCCCHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFLEKLSPIESKIKILEEKLQDINLVKNQKEYAKTVKEYNYLEKIKAKKDEYENILSQIN
CCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ENQKILSEEENLEMKELVKQELAHLNLKKDEVEHMIKILLLHQDENDSKNIIIEIRAGTG
CCHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCC
GEEAALFAHNLYEMYTKYSEKKKWKTELINFNETELGGFKEVSFEIKGKDVFKKLKHESG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHHHHCC
VHRVQRVPITESNGRLQTSAATVAVLPEVEDTDIEINEKDLRIDVYRSSGAGGQHVNTTD
HHHHHCCCCCCCCCEEEECCEEEEEECCCCCCCCEECCCEEEEEEEECCCCCCCCCCCCC
SAVRITHLPTGIVVQCQNERSQHKNKDQAMKILRARLYEFENLKKQEQRSNDRKQQVGSG
CEEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCC
DRSERIRTYNFPQNRVTDHRANISLYKLEEIMQGELDFLLDTLALKFQEQSLKDNSI
CCCCCCEECCCCCHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA