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The map label for this gene is priA [H]

Identifier: 187917897

GI number: 187917897

Start: 13047

End: 15023

Strand: Direct

Name: priA [H]

Synonym: BH0014

Alternate gene names: 187917897

Gene position: 13047-15023 (Clockwise)

Preceding gene: 187917896

Following gene: 187917900

Centisome position: 1.41

GC content: 27.47

Gene sequence:

>1977_bases
ATGGTTGATAGTTTGGAAAACAATTTTTACTATGAAATTGCGTTTAATATTCCCATAAATAGACTTTTTTTTTATAAGTA
TAAATCAAAATTAAAAACAGGAATAAGAGTAATAACAAATTTTAATGGAAAAGACACAATTGGAATCATAATCAAAAGAT
ATGCTAAGGAAGAACTTAAGGAAGATTTTACGTTTAAAATAAAAGATATACTAAAAGTCATTGACGAGAGCGCAATAATA
ATAGAACATAATATTAATCTTGCACGCTGGATTAGTCAAAAAACATTTTCTGGATTCGGAGAAACTCTATTTTGTGGGCT
ACCTAAAATTGCAACTTCAAATAAAGGCATAAAAAATAACGACAATGAAAATTTAAACTCTAAACTACCCATTCAACTAA
ATGAAGAACAAAATAATATTTATAAAGAAATTATTGCATCAAATACACAAAACACATATTACCTATTCGGGGTTCCTGGT
TCTGGAAAAACAGAAATATTTATCAAACTATGCGAAAACTATCTAGAACAAGAAAAACAAATAATTTTCTTAATTCCTGA
AATTTCTTTGGGCTATCAAATAATTAAAAGGATCAAATCAAATTTAAGTACAAATAAAGTTTATGAATATAACTCAAAAG
TATCAAATTCAAATAAAGCCTTAATATGGAACAAAGTCAAGAATAGGGAAAGCTTAATTATAATTGGTGTTAAGAGTGCA
TTAATGTTGCCATTTACAAACTTAGGGTTAATAGTAATGGACGAAGAACATGAATATACATATAAATCTGAAAACACTCC
AAGATTTCACTCAAGACATATAGGATTTTTTCTACAAAGCACCTTTAATGCCAAATTTATAATGGGAAGCGCAACTCCAT
CACTTGAAGCATATCTTGCTATGGAAAATAATCAGATCAAAAAAATGATTTTGAAAAATAAATTTTTTGAAAGAACATTT
AAAGAACTTAAAATAATCGACATGAAAAAAGAACGCCAATTAATATCTTCAGAATTGCTTTATAGCATACAAAAAAGTTT
GCTTGATAAAAGACAAGCACTAATATTTATTAATAAAAGAGGATATTCAAAAACACTTGAATGCAAGATCTGTGGATATA
TAATTTGCTGTCCAAATTGTTCTTTCAATTTAACTTACCATAAAAACGAAAACAAGCTCATCTGTCATTACTGTAGTTAT
AAAACAAACATAATAAGCAATTGCCCTGATTGCAACTCACAAGACATCATATACAAAACATATGGAATTCAATTCATTGA
AAAAGAATTAAAAAAATTTCTACCGAATGCAAGAATAGCAAGAACAGACTCTGATCTTAATAAAAAAGAAATTATTCAAT
CAATAAATGCATTTGAAAATGGGCAATTAGATATCTTAATTGGAACACAAATTATTGCAAAAGGTTTTAATTTTAAAGAG
ATCAAAACGCTAGGCATCATTAATGCAGATATTGGAATGGGTCTTCCTGATTTTAGAAGCAGTGAAAGAATTTTTGCAAT
AATTTCACAAGTACTAGGAAGAGCTGCAAGATTCCAAAGCGATAACACAATTATTATCCAAACAAAAAACCCAGATTATT
ATGCTATAAAATACGCATATGAGGGCAAATACGAAGAATTTTATAAAGAAGAGATAAAAATTCGCAAAGAATTAAATTAC
CCTCCCTTTAAAAAAATCATTAGGATAGTAGTTAGAAGCCACAAACAGGATGCTGCTAAACATAAATGCCTAGAATTTTT
TGAAATATCTAAAAAATTATTAAATGACGAAATCGAATATCTTGGCCCATCAAAAGCCCCCATGTCAAAAATATCCAAAT
ACTATAGGTATAACATAATATATTTATCAAAATCCTTCAATCTACTTGAAAAACTAATACGTAACACAAAAGAAAAAGTA
AAATTAACAAGAGATACCTACATTGAAATAGATTATTATCCAATTTCACTGCTTTAA

Upstream 100 bases:

>100_bases
CCAATAGTATTTACATATCTTCCTACAAGTCAAGTAACAAATCCAGAACTCAAGAAAGAAATCTGGCAAGACTTAAAAAT
AATAAAAGGCATAATAAAAT

Downstream 100 bases:

>100_bases
AAAAAATCTGCACCCTCCTTCCCAAGAGTTCTTAAGAAAGATGAGAGGACATAAAGAGCCTTATCATTATGTCCTCCTTC
AGGAATAATAAGGTCAGCAT

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 658; Mature: 658

Protein sequence:

>658_residues
MVDSLENNFYYEIAFNIPINRLFFYKYKSKLKTGIRVITNFNGKDTIGIIIKRYAKEELKEDFTFKIKDILKVIDESAII
IEHNINLARWISQKTFSGFGETLFCGLPKIATSNKGIKNNDNENLNSKLPIQLNEEQNNIYKEIIASNTQNTYYLFGVPG
SGKTEIFIKLCENYLEQEKQIIFLIPEISLGYQIIKRIKSNLSTNKVYEYNSKVSNSNKALIWNKVKNRESLIIIGVKSA
LMLPFTNLGLIVMDEEHEYTYKSENTPRFHSRHIGFFLQSTFNAKFIMGSATPSLEAYLAMENNQIKKMILKNKFFERTF
KELKIIDMKKERQLISSELLYSIQKSLLDKRQALIFINKRGYSKTLECKICGYIICCPNCSFNLTYHKNENKLICHYCSY
KTNIISNCPDCNSQDIIYKTYGIQFIEKELKKFLPNARIARTDSDLNKKEIIQSINAFENGQLDILIGTQIIAKGFNFKE
IKTLGIINADIGMGLPDFRSSERIFAIISQVLGRAARFQSDNTIIIQTKNPDYYAIKYAYEGKYEEFYKEEIKIRKELNY
PPFKKIIRIVVRSHKQDAAKHKCLEFFEISKKLLNDEIEYLGPSKAPMSKISKYYRYNIIYLSKSFNLLEKLIRNTKEKV
KLTRDTYIEIDYYPISLL

Sequences:

>Translated_658_residues
MVDSLENNFYYEIAFNIPINRLFFYKYKSKLKTGIRVITNFNGKDTIGIIIKRYAKEELKEDFTFKIKDILKVIDESAII
IEHNINLARWISQKTFSGFGETLFCGLPKIATSNKGIKNNDNENLNSKLPIQLNEEQNNIYKEIIASNTQNTYYLFGVPG
SGKTEIFIKLCENYLEQEKQIIFLIPEISLGYQIIKRIKSNLSTNKVYEYNSKVSNSNKALIWNKVKNRESLIIIGVKSA
LMLPFTNLGLIVMDEEHEYTYKSENTPRFHSRHIGFFLQSTFNAKFIMGSATPSLEAYLAMENNQIKKMILKNKFFERTF
KELKIIDMKKERQLISSELLYSIQKSLLDKRQALIFINKRGYSKTLECKICGYIICCPNCSFNLTYHKNENKLICHYCSY
KTNIISNCPDCNSQDIIYKTYGIQFIEKELKKFLPNARIARTDSDLNKKEIIQSINAFENGQLDILIGTQIIAKGFNFKE
IKTLGIINADIGMGLPDFRSSERIFAIISQVLGRAARFQSDNTIIIQTKNPDYYAIKYAYEGKYEEFYKEEIKIRKELNY
PPFKKIIRIVVRSHKQDAAKHKCLEFFEISKKLLNDEIEYLGPSKAPMSKISKYYRYNIIYLSKSFNLLEKLIRNTKEKV
KLTRDTYIEIDYYPISLL
>Mature_658_residues
MVDSLENNFYYEIAFNIPINRLFFYKYKSKLKTGIRVITNFNGKDTIGIIIKRYAKEELKEDFTFKIKDILKVIDESAII
IEHNINLARWISQKTFSGFGETLFCGLPKIATSNKGIKNNDNENLNSKLPIQLNEEQNNIYKEIIASNTQNTYYLFGVPG
SGKTEIFIKLCENYLEQEKQIIFLIPEISLGYQIIKRIKSNLSTNKVYEYNSKVSNSNKALIWNKVKNRESLIIIGVKSA
LMLPFTNLGLIVMDEEHEYTYKSENTPRFHSRHIGFFLQSTFNAKFIMGSATPSLEAYLAMENNQIKKMILKNKFFERTF
KELKIIDMKKERQLISSELLYSIQKSLLDKRQALIFINKRGYSKTLECKICGYIICCPNCSFNLTYHKNENKLICHYCSY
KTNIISNCPDCNSQDIIYKTYGIQFIEKELKKFLPNARIARTDSDLNKKEIIQSINAFENGQLDILIGTQIIAKGFNFKE
IKTLGIINADIGMGLPDFRSSERIFAIISQVLGRAARFQSDNTIIIQTKNPDYYAIKYAYEGKYEEFYKEEIKIRKELNY
PPFKKIIRIVVRSHKQDAAKHKCLEFFEISKKLLNDEIEYLGPSKAPMSKISKYYRYNIIYLSKSFNLLEKLIRNTKEKV
KLTRDTYIEIDYYPISLL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=513, Percent_Identity=29.8245614035088, Blast_Score=293, Evalue=3e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259
- InterPro:   IPR006935 [H]

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII [H]

EC number: NA

Molecular weight: Translated: 76793; Mature: 76793

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDSLENNFYYEIAFNIPINRLFFYKYKSKLKTGIRVITNFNGKDTIGIIIKRYAKEELK
CCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHH
EDFTFKIKDILKVIDESAIIIEHNINLARWISQKTFSGFGETLFCGLPKIATSNKGIKNN
HCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC
DNENLNSKLPIQLNEEQNNIYKEIIASNTQNTYYLFGVPGSGKTEIFIKLCENYLEQEKQ
CCCCCCCCCCEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCE
IIFLIPEISLGYQIIKRIKSNLSTNKVYEYNSKVSNSNKALIWNKVKNRESLIIIGVKSA
EEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEECCCCCCEEEEEECCC
LMLPFTNLGLIVMDEEHEYTYKSENTPRFHSRHIGFFLQSTFNAKFIMGSATPSLEAYLA
EECCCCCCCEEEEECCCCCEECCCCCCCHHHHHHEEEEECCCCEEEEEECCCCCCEEEEE
MENNQIKKMILKNKFFERTFKELKIIDMKKERQLISSELLYSIQKSLLDKRQALIFINKR
ECCCHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
GYSKTLECKICGYIICCPNCSFNLTYHKNENKLICHYCSYKTNIISNCPDCNSQDIIYKT
CCCCEEEEEEEEEEEECCCCCEEEEEEECCCCEEEEEECCCCHHHCCCCCCCCCCEEEEE
YGIQFIEKELKKFLPNARIARTDSDLNKKEIIQSINAFENGQLDILIGTQIIAKGFNFKE
HHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCCCEEEEECHHHHHCCCCHHH
IKTLGIINADIGMGLPDFRSSERIFAIISQVLGRAARFQSDNTIIIQTKNPDYYAIKYAY
EEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEE
EGKYEEFYKEEIKIRKELNYPPFKKIIRIVVRSHKQDAAKHKCLEFFEISKKLLNDEIEY
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHHH
LGPSKAPMSKISKYYRYNIIYLSKSFNLLEKLIRNTKEKVKLTRDTYIEIDYYPISLL
CCCCCCHHHHHHHHHEEEEEEEECCHHHHHHHHHCCHHHEEEEECEEEEEEEEEEEEC
>Mature Secondary Structure
MVDSLENNFYYEIAFNIPINRLFFYKYKSKLKTGIRVITNFNGKDTIGIIIKRYAKEELK
CCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHH
EDFTFKIKDILKVIDESAIIIEHNINLARWISQKTFSGFGETLFCGLPKIATSNKGIKNN
HCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC
DNENLNSKLPIQLNEEQNNIYKEIIASNTQNTYYLFGVPGSGKTEIFIKLCENYLEQEKQ
CCCCCCCCCCEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCE
IIFLIPEISLGYQIIKRIKSNLSTNKVYEYNSKVSNSNKALIWNKVKNRESLIIIGVKSA
EEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEECCCCCCEEEEEECCC
LMLPFTNLGLIVMDEEHEYTYKSENTPRFHSRHIGFFLQSTFNAKFIMGSATPSLEAYLA
EECCCCCCCEEEEECCCCCEECCCCCCCHHHHHHEEEEECCCCEEEEEECCCCCCEEEEE
MENNQIKKMILKNKFFERTFKELKIIDMKKERQLISSELLYSIQKSLLDKRQALIFINKR
ECCCHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
GYSKTLECKICGYIICCPNCSFNLTYHKNENKLICHYCSYKTNIISNCPDCNSQDIIYKT
CCCCEEEEEEEEEEEECCCCCEEEEEEECCCCEEEEEECCCCHHHCCCCCCCCCCEEEEE
YGIQFIEKELKKFLPNARIARTDSDLNKKEIIQSINAFENGQLDILIGTQIIAKGFNFKE
HHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCCCEEEEECHHHHHCCCCHHH
IKTLGIINADIGMGLPDFRSSERIFAIISQVLGRAARFQSDNTIIIQTKNPDYYAIKYAY
EEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEE
EGKYEEFYKEEIKIRKELNYPPFKKIIRIVVRSHKQDAAKHKCLEFFEISKKLLNDEIEY
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHHH
LGPSKAPMSKISKYYRYNIIYLSKSFNLLEKLIRNTKEKVKLTRDTYIEIDYYPISLL
CCCCCCHHHHHHHHHEEEEEEEECCHHHHHHHHHCCHHHEEEEECEEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9228761; 9403685 [H]