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The map label for this gene is pyrE [H]

Identifier: 187736588

GI number: 187736588

Start: 2570344

End: 2570913

Strand: Reverse

Name: pyrE [H]

Synonym: Amuc_2110

Alternate gene names: 187736588

Gene position: 2570913-2570344 (Counterclockwise)

Preceding gene: 187736592

Following gene: 187736587

Centisome position: 96.5

GC content: 59.3

Gene sequence:

>570_bases
ATGAGCGAACAATACGATCTTCTTAAAAGCATTCTTTTGGAAAAATCCGTGCGTACGGGAACTTTTACACTGGCTTCCGG
CAAGGTGTCCGATTTGTATGTGGACTGCCGCATGACAGCCCTGGACCCCGTAGGAGCCACTCTGGTGGGCGCTCTGGGCT
GGAAGCTGGTGAAGGAGGATATTTTGCCCCGTTTCCCGGAGGTGGCGGCCATCGGCGGCATGACCATGGGGGCGGACCCT
ATTTCCCTGGCCGTCGGCATGACCAGCGCTCTGGAAGGGGCGGAAAAGACGCTTCAGGTGTTCACCGTGCGCAAAGAACC
GAAGGACCATGGCCGCGGCCGCCGTATTGAAGGCAATTTCCAGGCCGGGGATACCGTGGTCGTGGTGGATGACGTTATCA
CGACGGGAGGCTCTACGCTGAAGGCTATTGATGCCATTGAGGCGGAAGGGGGCAGGGTTGCCGCCGCCCTGGTGCTGCTG
GACCGAGAGGAAGGCGGACGCCAGGCGATTGAGGAACGCGGCATTCCCGTTTACCCGCTGTACACCAGGACTTCCTTGTT
GGGCCATTAA

Upstream 100 bases:

>100_bases
GCCTGTCCTCCTTTTTGTCAAGATTTGCATGCTGTTATCCGTTGCTTATTCCGGGGGTATCTGTTATACACGGCGAACCA
TTTCATTCATCCCTATTTTC

Downstream 100 bases:

>100_bases
TCTTTTTTCGTCCATGCTCCCTGCCCTGATCGGCATAAGCGGCCATGAAGTGGGCGCGGAGGAGGAGGCTGCCATCCGGC
GTTTGCAGCCGGCCGGATTC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MSEQYDLLKSILLEKSVRTGTFTLASGKVSDLYVDCRMTALDPVGATLVGALGWKLVKEDILPRFPEVAAIGGMTMGADP
ISLAVGMTSALEGAEKTLQVFTVRKEPKDHGRGRRIEGNFQAGDTVVVVDDVITTGGSTLKAIDAIEAEGGRVAAALVLL
DREEGGRQAIEERGIPVYPLYTRTSLLGH

Sequences:

>Translated_189_residues
MSEQYDLLKSILLEKSVRTGTFTLASGKVSDLYVDCRMTALDPVGATLVGALGWKLVKEDILPRFPEVAAIGGMTMGADP
ISLAVGMTSALEGAEKTLQVFTVRKEPKDHGRGRRIEGNFQAGDTVVVVDDVITTGGSTLKAIDAIEAEGGRVAAALVLL
DREEGGRQAIEERGIPVYPLYTRTSLLGH
>Mature_188_residues
SEQYDLLKSILLEKSVRTGTFTLASGKVSDLYVDCRMTALDPVGATLVGALGWKLVKEDILPRFPEVAAIGGMTMGADPI
SLAVGMTSALEGAEKTLQVFTVRKEPKDHGRGRRIEGNFQAGDTVVVVDDVITTGGSTLKAIDAIEAEGGRVAAALVLLD
REEGGRQAIEERGIPVYPLYTRTSLLGH

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=173, Percent_Identity=30.0578034682081, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17554964, Length=182, Percent_Identity=28.021978021978, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17508631, Length=186, Percent_Identity=29.0322580645161, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17933654, Length=192, Percent_Identity=27.6041666666667, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 20129; Mature: 19998

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQYDLLKSILLEKSVRTGTFTLASGKVSDLYVDCRMTALDPVGATLVGALGWKLVKED
CCHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHH
ILPRFPEVAAIGGMTMGADPISLAVGMTSALEGAEKTLQVFTVRKEPKDHGRGRRIEGNF
HCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCEECCCC
QAGDTVVVVDDVITTGGSTLKAIDAIEAEGGRVAAALVLLDREEGGRQAIEERGIPVYPL
CCCCEEEEEECEECCCCCHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHCCCCEEEH
YTRTSLLGH
HHHHHHCCC
>Mature Secondary Structure 
SEQYDLLKSILLEKSVRTGTFTLASGKVSDLYVDCRMTALDPVGATLVGALGWKLVKED
CHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHH
ILPRFPEVAAIGGMTMGADPISLAVGMTSALEGAEKTLQVFTVRKEPKDHGRGRRIEGNF
HCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCEECCCC
QAGDTVVVVDDVITTGGSTLKAIDAIEAEGGRVAAALVLLDREEGGRQAIEERGIPVYPL
CCCCEEEEEECEECCCCCHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHCCCCEEEH
YTRTSLLGH
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]