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The map label for this gene is menD

Identifier: 187736582

GI number: 187736582

Start: 2558514

End: 2560115

Strand: Direct

Name: menD

Synonym: Amuc_2104

Alternate gene names: 187736582

Gene position: 2558514-2560115 (Clockwise)

Preceding gene: 187736581

Following gene: 187736583

Centisome position: 96.04

GC content: 65.17

Gene sequence:

>1602_bases
GTGAATTCCCCCGCATCCTTCGTCAAATCCCTGCTGGCCCAATGCTGCCTGGGCGGCATTTGCGAGTGGGTGGTCTGCCC
CGGCGCGCGCAACATGGCGCTGCTCCAGGTGCTGGCTGCCGCGGAGGATCTGGTGAAATGGACTCACTTTGACGAACGGT
CCGCCGCCTTCTTTGCGCTGGGCCGCATTCAGGATATAGGGCTGCCCGTGGCCGTGGTCACTACCTCCGGAACGGCGGCG
GCGGAACTGCTCCCCGCCGTGGTGGAGGCCTACTACCAGCGCCGCCCCCTGCTCCTGCTCACGGCGGACCGTCCGGCGGC
CTGCCGGGGTTCCGCCGCTCCCCAGGCCATCGAACAGGCGGATCTGTTCGGCATTTACGCACCCACGATTGACCTGGAAA
CGCCGGAAAGCCTGCCGGAAGACATTCTGCAAGACTGGGATTACGCCTCACCCCTCCATATCAACGTGTGCCTGCCGGAT
CCGGACCCTGCCTGGAACCCCGGCAGCTGCGACCTTTACCCGGCGGAACCTCCGGAGGAAAACGGATTCCGGGGCTCCCT
GGCAGAACTGGCCCGGGCCCTGCGCTTCAAATCGCGCGGCGGGCTGGTGGTCATGATCGGCGGCCTGGACCCCACGGAAC
AAGCCCCGGCCCGGTGGCTGGCCAACGAACTGAAAGCCCCCGTGGTGGCGGACGCCACCTCCGGACTCCGGGAAGAGCTG
GCCCATCTGGCTCTGACGGACGCAGACGCCCTGCTGAGGGAACACCCGCCCGCCGTCCTGCTCAGATTAGGGGACGTGCC
CGTGGCCCGCTTCTGGCGCGACCTGGAGGACATTCCAGCCACGGAAGTCTTCTCCGTCACCCGCACCGGCTTCTCCGGGC
TGGCCCGCCCCTCCTCCGTCGTAACCGGAGACCTGGAAGCCATTCTGCATGCGCTGGGGGATATAGATACCGTGGGCGAC
GTCAACGGCCTGCGCGCCATGAACAAACGCAGGAAAGCCCTGATGGAGGAACTGCTCATCACCTGCCCGGAAAGCGAACA
GGCCATGGTGCGCTCCTTCTCCTGCTTTGCCGCGGACGGAGACTGCATTTACCTGGGCAACTCCATGCCCGTGCGGTACT
GGAACAGCTTCGCCCAGACGTCCATCCCCACGGAAAACGTCCGCGCCAACCGGGGCACCAACGGCATTGACGGGCAAATC
TCCGGTTTTCTGGGGGTCTCCGCCCGGTGTTCCCGCTCCTGGGCTCTCGTGGGCGACCTGACCGCCATGTATGATTCCAA
TGCCCTGGCACTCCTTCCCCAACTGGACAGGGGGACCCGCGTCCTCGGCGTCATCAACAACGGCGGAGGGGGCATCTTCC
GCACCCTCCCGGGAGCAGACGGCCAGCCGGAAACCATGAGGAAGCTGCTTGTACAACCCCACGCCCATTCCTTCAAAGCC
ATTGCGGAACAATGGGGAATGCGCTACCTGACCATCCGCACGGCGGAGGACTTCGACCAGCTTGATTCCCTGGAGGAAAA
CAGCCAGACGCTTGTAGAGCTCATCCCGGACAGGGAGCAGACGGAACAGATCCGCCTCCGGCTGGCCAACGCCCAGGTGT
AA

Upstream 100 bases:

>100_bases
ATGATCCCGCCCCCGTTTTTCCCCGCATTCTTTCGGAAGAGGAAAAGGCCAAACTGGCTGAAAAAGCGGCCAGGGCGGCA
AAAAAACAAGGCTGATTCCC

Downstream 100 bases:

>100_bases
ACATGCCTTCACCAAAAACGAAAGGAAGCCGGCCTTTTGCCCGGCCATATCCTGCGATTCCTTCCAAATGGATTGAAAAT
GCCAGATAAAAATAACATTT

Product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MNSPASFVKSLLAQCCLGGICEWVVCPGARNMALLQVLAAAEDLVKWTHFDERSAAFFALGRIQDIGLPVAVVTTSGTAA
AELLPAVVEAYYQRRPLLLLTADRPAACRGSAAPQAIEQADLFGIYAPTIDLETPESLPEDILQDWDYASPLHINVCLPD
PDPAWNPGSCDLYPAEPPEENGFRGSLAELARALRFKSRGGLVVMIGGLDPTEQAPARWLANELKAPVVADATSGLREEL
AHLALTDADALLREHPPAVLLRLGDVPVARFWRDLEDIPATEVFSVTRTGFSGLARPSSVVTGDLEAILHALGDIDTVGD
VNGLRAMNKRRKALMEELLITCPESEQAMVRSFSCFAADGDCIYLGNSMPVRYWNSFAQTSIPTENVRANRGTNGIDGQI
SGFLGVSARCSRSWALVGDLTAMYDSNALALLPQLDRGTRVLGVINNGGGGIFRTLPGADGQPETMRKLLVQPHAHSFKA
IAEQWGMRYLTIRTAEDFDQLDSLEENSQTLVELIPDREQTEQIRLRLANAQV

Sequences:

>Translated_533_residues
MNSPASFVKSLLAQCCLGGICEWVVCPGARNMALLQVLAAAEDLVKWTHFDERSAAFFALGRIQDIGLPVAVVTTSGTAA
AELLPAVVEAYYQRRPLLLLTADRPAACRGSAAPQAIEQADLFGIYAPTIDLETPESLPEDILQDWDYASPLHINVCLPD
PDPAWNPGSCDLYPAEPPEENGFRGSLAELARALRFKSRGGLVVMIGGLDPTEQAPARWLANELKAPVVADATSGLREEL
AHLALTDADALLREHPPAVLLRLGDVPVARFWRDLEDIPATEVFSVTRTGFSGLARPSSVVTGDLEAILHALGDIDTVGD
VNGLRAMNKRRKALMEELLITCPESEQAMVRSFSCFAADGDCIYLGNSMPVRYWNSFAQTSIPTENVRANRGTNGIDGQI
SGFLGVSARCSRSWALVGDLTAMYDSNALALLPQLDRGTRVLGVINNGGGGIFRTLPGADGQPETMRKLLVQPHAHSFKA
IAEQWGMRYLTIRTAEDFDQLDSLEENSQTLVELIPDREQTEQIRLRLANAQV
>Mature_533_residues
MNSPASFVKSLLAQCCLGGICEWVVCPGARNMALLQVLAAAEDLVKWTHFDERSAAFFALGRIQDIGLPVAVVTTSGTAA
AELLPAVVEAYYQRRPLLLLTADRPAACRGSAAPQAIEQADLFGIYAPTIDLETPESLPEDILQDWDYASPLHINVCLPD
PDPAWNPGSCDLYPAEPPEENGFRGSLAELARALRFKSRGGLVVMIGGLDPTEQAPARWLANELKAPVVADATSGLREEL
AHLALTDADALLREHPPAVLLRLGDVPVARFWRDLEDIPATEVFSVTRTGFSGLARPSSVVTGDLEAILHALGDIDTVGD
VNGLRAMNKRRKALMEELLITCPESEQAMVRSFSCFAADGDCIYLGNSMPVRYWNSFAQTSIPTENVRANRGTNGIDGQI
SGFLGVSARCSRSWALVGDLTAMYDSNALALLPQLDRGTRVLGVINNGGGGIFRTLPGADGQPETMRKLLVQPHAHSFKA
IAEQWGMRYLTIRTAEDFDQLDSLEENSQTLVELIPDREQTEQIRLRLANAQV

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=542, Percent_Identity=28.5977859778598, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_AKKM8 (B2UPN5)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001878694.1
- ProteinModelPortal:   B2UPN5
- GeneID:   6273696
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_2104
- OMA:   FYHDLNG
- ProtClustDB:   CLSK736866
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 57783; Mature: 57783

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPASFVKSLLAQCCLGGICEWVVCPGARNMALLQVLAAAEDLVKWTHFDERSAAFFAL
CCCHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
GRIQDIGLPVAVVTTSGTAAAELLPAVVEAYYQRRPLLLLTADRPAACRGSAAPQAIEQA
HHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHH
DLFGIYAPTIDLETPESLPEDILQDWDYASPLHINVCLPDPDPAWNPGSCDLYPAEPPEE
HHEEEEECEECCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCC
NGFRGSLAELARALRFKSRGGLVVMIGGLDPTEQAPARWLANELKAPVVADATSGLREEL
CCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHH
AHLALTDADALLREHPPAVLLRLGDVPVARFWRDLEDIPATEVFSVTRTGFSGLARPSSV
HHHHHCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHH
VTGDLEAILHALGDIDTVGDVNGLRAMNKRRKALMEELLITCPESEQAMVRSFSCFAADG
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEECCC
DCIYLGNSMPVRYWNSFAQTSIPTENVRANRGTNGIDGQISGFLGVSARCSRSWALVGDL
CEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCEEEHHHH
TAMYDSNALALLPQLDRGTRVLGVINNGGGGIFRTLPGADGQPETMRKLLVQPHAHSFKA
HHHHCCCCEEEEECCCCCCEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHHH
IAEQWGMRYLTIRTAEDFDQLDSLEENSQTLVELIPDREQTEQIRLRLANAQV
HHHHHCCEEEEEECHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNSPASFVKSLLAQCCLGGICEWVVCPGARNMALLQVLAAAEDLVKWTHFDERSAAFFAL
CCCHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
GRIQDIGLPVAVVTTSGTAAAELLPAVVEAYYQRRPLLLLTADRPAACRGSAAPQAIEQA
HHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHH
DLFGIYAPTIDLETPESLPEDILQDWDYASPLHINVCLPDPDPAWNPGSCDLYPAEPPEE
HHEEEEECEECCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCC
NGFRGSLAELARALRFKSRGGLVVMIGGLDPTEQAPARWLANELKAPVVADATSGLREEL
CCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHH
AHLALTDADALLREHPPAVLLRLGDVPVARFWRDLEDIPATEVFSVTRTGFSGLARPSSV
HHHHHCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHH
VTGDLEAILHALGDIDTVGDVNGLRAMNKRRKALMEELLITCPESEQAMVRSFSCFAADG
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEECCC
DCIYLGNSMPVRYWNSFAQTSIPTENVRANRGTNGIDGQISGFLGVSARCSRSWALVGDL
CEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCEEEHHHH
TAMYDSNALALLPQLDRGTRVLGVINNGGGGIFRTLPGADGQPETMRKLLVQPHAHSFKA
HHHHCCCCEEEEECCCCCCEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHHH
IAEQWGMRYLTIRTAEDFDQLDSLEENSQTLVELIPDREQTEQIRLRLANAQV
HHHHHCCEEEEEECHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA