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The map label for this gene is metG [H]

Identifier: 187736581

GI number: 187736581

Start: 2556856

End: 2558508

Strand: Direct

Name: metG [H]

Synonym: Amuc_2103

Alternate gene names: 187736581

Gene position: 2556856-2558508 (Clockwise)

Preceding gene: 187736580

Following gene: 187736582

Centisome position: 95.97

GC content: 59.47

Gene sequence:

>1653_bases
ATGACTTTGCTTGCAAAACGGCAGGAAAGGAGTATCGTCCCCCGCATGTACTATATCACCACGGCCATTGACTACACCAA
CGGACCGCCCCACATCGGGCACGCTTATGAAAAAGTCCTGGCGGACGTCCTCGCGCGCTGGCACCGGATGAAGGGGGAGG
AAGTCTATTTCCTGACCGGGGTGGACCAGCATGGGCAGAAAGTGCAGCAGACGGCGGAAAAGCTGGGCATTACGCCGCAG
GAGCATGTCAACAATATCACAGGGCAATTCCTGGCGTTGTGGGACCGACTGGGCATCAGCAATGACGGATGGGCCTCCAC
GACGGATCCGCGCCACAAGGCCTGCGTGCAGAAAATCCTGACGGACCTGAAGGACAAGGGGCAGCTTTACAAAAAATCCT
ACAAGGGATTCTACTCCGTGCGCCAGGAACAGTTCCTGACGGACAAGGAACGGGACGCGGAAGGCAACTTCGGCCCGGAA
TGGGGGGAAGTGGTGGAACTGGAGGAAGAAAACTGGTATTTCCGCCTGACCGACCATGTGGAATGGATGAAGCAATTCGT
GGAAAAGAGCAGTGATTTTGTCCTTCCGTCCTTCCGCAAGGCGGAAGTGCTCAACGCCATTGACTTTGACTCGGACCTCT
GCATTTCCCGCCCCAAGAGCCGCCTTTCCTGGGGAATCGAATTCCCGTTCGACCCCGAATTCGTCACCTACGTCTGGTTT
GACGCCCTGATCAACTACATTTCCTTCGCCGGCTACCTGGCGGAGCCGGACAGCGGCCTGCCGGAATTCTCCAAACTCTG
GCCCGCAGACTGCGAAGTCATCGGCAAAGACATCCTGGTGCCCGCCCACGGCGTGTACTGGCCCGCCATGCTGCATGCCA
TGGGCTTTTCCGACGAGGAAATGCCCACCCTGCTCGTGCACGGCTGGTGGAACATCAACGGGGAAAAAATGTCCAAGTCC
ATCGGCAACGTGGTGGACCCCAACCTGCTGGCGGAACAATTCGGCCCGGAACCTGTGCGCTATTACCTGGTGCGGGACAT
CACCACCGGGAAGGACGCCAACTTTGACGCGGACCGCTTGGTCATGCTGTACAATACGGAGCTGGCCAACGACCTCGGCA
ACCTGTGCAACCGCTCCATCAACATGACGCGCCGCTATTGCGACTCCGTTATCTCCGGAGCGGAGGCATATGATGACGAA
GCCTCCAAAGCCCTGCGCTCCACCATGGACCAGGCCGTCACCCTGTTTTCCAAATACATGGACGACAACATGGCCTCCGA
TGCCCTGGCGGCCCTGAACGCCCAGGTTTCCGCCTGTAACGCGTACATTGAGCAGCAGCAGCCCTGGCAGCTGGCCAAGG
ATGAAGCCTCCGCCCCGCGCCTCCGCTGCGTGCTGCGCCATTTGCTGGAATGCTGCGCCCAAACGGGCTATCTCATCGGC
TGCGTCCTCCCTGACGCCTCCGCCCGCATTCTGGGCCAGCTCAACGCCGGGGATCTGTTCCGGGGCCTTACGCCGGCCGC
GCTGAAGTGGGGAATCCTCCCCGCAGGGCACCGCATCAATGATCCCGCCCCCGTTTTTCCCCGCATTCTTTCGGAAGAGG
AAAAGGCCAAACTGGCTGAAAAAGCGGCCAGGGCGGCAAAAAAACAAGGCTGA

Upstream 100 bases:

>100_bases
CGGTGGAAAGGGAATATCTGAACAACCCCTTCCGCCGTTTCGCCCGCGGCCCCCCCTTACTCTCCCATCCCATGCTGAAA
AACACGCCCGAGATGCCGCC

Downstream 100 bases:

>100_bases
TTCCCGTGAATTCCCCCGCATCCTTCGTCAAATCCCTGCTGGCCCAATGCTGCCTGGGCGGCATTTGCGAGTGGGTGGTC
TGCCCCGGCGCGCGCAACAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MTLLAKRQERSIVPRMYYITTAIDYTNGPPHIGHAYEKVLADVLARWHRMKGEEVYFLTGVDQHGQKVQQTAEKLGITPQ
EHVNNITGQFLALWDRLGISNDGWASTTDPRHKACVQKILTDLKDKGQLYKKSYKGFYSVRQEQFLTDKERDAEGNFGPE
WGEVVELEEENWYFRLTDHVEWMKQFVEKSSDFVLPSFRKAEVLNAIDFDSDLCISRPKSRLSWGIEFPFDPEFVTYVWF
DALINYISFAGYLAEPDSGLPEFSKLWPADCEVIGKDILVPAHGVYWPAMLHAMGFSDEEMPTLLVHGWWNINGEKMSKS
IGNVVDPNLLAEQFGPEPVRYYLVRDITTGKDANFDADRLVMLYNTELANDLGNLCNRSINMTRRYCDSVISGAEAYDDE
ASKALRSTMDQAVTLFSKYMDDNMASDALAALNAQVSACNAYIEQQQPWQLAKDEASAPRLRCVLRHLLECCAQTGYLIG
CVLPDASARILGQLNAGDLFRGLTPAALKWGILPAGHRINDPAPVFPRILSEEEKAKLAEKAARAAKKQG

Sequences:

>Translated_550_residues
MTLLAKRQERSIVPRMYYITTAIDYTNGPPHIGHAYEKVLADVLARWHRMKGEEVYFLTGVDQHGQKVQQTAEKLGITPQ
EHVNNITGQFLALWDRLGISNDGWASTTDPRHKACVQKILTDLKDKGQLYKKSYKGFYSVRQEQFLTDKERDAEGNFGPE
WGEVVELEEENWYFRLTDHVEWMKQFVEKSSDFVLPSFRKAEVLNAIDFDSDLCISRPKSRLSWGIEFPFDPEFVTYVWF
DALINYISFAGYLAEPDSGLPEFSKLWPADCEVIGKDILVPAHGVYWPAMLHAMGFSDEEMPTLLVHGWWNINGEKMSKS
IGNVVDPNLLAEQFGPEPVRYYLVRDITTGKDANFDADRLVMLYNTELANDLGNLCNRSINMTRRYCDSVISGAEAYDDE
ASKALRSTMDQAVTLFSKYMDDNMASDALAALNAQVSACNAYIEQQQPWQLAKDEASAPRLRCVLRHLLECCAQTGYLIG
CVLPDASARILGQLNAGDLFRGLTPAALKWGILPAGHRINDPAPVFPRILSEEEKAKLAEKAARAAKKQG
>Mature_549_residues
TLLAKRQERSIVPRMYYITTAIDYTNGPPHIGHAYEKVLADVLARWHRMKGEEVYFLTGVDQHGQKVQQTAEKLGITPQE
HVNNITGQFLALWDRLGISNDGWASTTDPRHKACVQKILTDLKDKGQLYKKSYKGFYSVRQEQFLTDKERDAEGNFGPEW
GEVVELEEENWYFRLTDHVEWMKQFVEKSSDFVLPSFRKAEVLNAIDFDSDLCISRPKSRLSWGIEFPFDPEFVTYVWFD
ALINYISFAGYLAEPDSGLPEFSKLWPADCEVIGKDILVPAHGVYWPAMLHAMGFSDEEMPTLLVHGWWNINGEKMSKSI
GNVVDPNLLAEQFGPEPVRYYLVRDITTGKDANFDADRLVMLYNTELANDLGNLCNRSINMTRRYCDSVISGAEAYDDEA
SKALRSTMDQAVTLFSKYMDDNMASDALAALNAQVSACNAYIEQQQPWQLAKDEASAPRLRCVLRHLLECCAQTGYLIGC
VLPDASARILGQLNAGDLFRGLTPAALKWGILPAGHRINDPAPVFPRILSEEEKAKLAEKAARAAKKQG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=512, Percent_Identity=34.375, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI14043022, Length=587, Percent_Identity=24.0204429301533, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI1788432, Length=556, Percent_Identity=25.3597122302158, Blast_Score=167, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI212645096, Length=501, Percent_Identity=33.7325349301397, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI71992781, Length=394, Percent_Identity=32.48730964467, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17540794, Length=599, Percent_Identity=25.7095158597663, Blast_Score=137, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321610, Length=551, Percent_Identity=30.3085299455535, Blast_Score=226, Evalue=7e-60,
Organism=Saccharomyces cerevisiae, GI6321704, Length=575, Percent_Identity=24.8695652173913, Blast_Score=109, Evalue=9e-25,
Organism=Drosophila melanogaster, GI21358483, Length=569, Percent_Identity=32.1616871704745, Blast_Score=258, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24655311, Length=576, Percent_Identity=26.5625, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 62133; Mature: 62002

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLAKRQERSIVPRMYYITTAIDYTNGPPHIGHAYEKVLADVLARWHRMKGEEVYFLTG
CCCCCCCHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEC
VDQHGQKVQQTAEKLGITPQEHVNNITGQFLALWDRLGISNDGWASTTDPRHKACVQKIL
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
TDLKDKGQLYKKSYKGFYSVRQEQFLTDKERDAEGNFGPEWGEVVELEEENWYFRLTDHV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCEEEEHHHH
EWMKQFVEKSSDFVLPSFRKAEVLNAIDFDSDLCISRPKSRLSWGIEFPFDPEFVTYVWF
HHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCHHHCCCCCCCCCCCHHHHHHHH
DALINYISFAGYLAEPDSGLPEFSKLWPADCEVIGKDILVPAHGVYWPAMLHAMGFSDEE
HHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHCCCCEECCCCHHHHHHHHHHCCCCCC
MPTLLVHGWWNINGEKMSKSIGNVVDPNLLAEQFGPEPVRYYLVRDITTGKDANFDADRL
CCEEEEEEEECCCHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCE
VMLYNTELANDLGNLCNRSINMTRRYCDSVISGAEAYDDEASKALRSTMDQAVTLFSKYM
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
DDNMASDALAALNAQVSACNAYIEQQQPWQLAKDEASAPRLRCVLRHLLECCAQTGYLIG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEE
CVLPDASARILGQLNAGDLFRGLTPAALKWGILPAGHRINDPAPVFPRILSEEEKAKLAE
EECCCCHHHHHCCCCHHHHHHCCCHHHHHHCCCCCCCCCCCCCCHHHHHHCHHHHHHHHH
KAARAAKKQG
HHHHHHHCCC
>Mature Secondary Structure 
TLLAKRQERSIVPRMYYITTAIDYTNGPPHIGHAYEKVLADVLARWHRMKGEEVYFLTG
CCCCCCHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEC
VDQHGQKVQQTAEKLGITPQEHVNNITGQFLALWDRLGISNDGWASTTDPRHKACVQKIL
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
TDLKDKGQLYKKSYKGFYSVRQEQFLTDKERDAEGNFGPEWGEVVELEEENWYFRLTDHV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCEEEEHHHH
EWMKQFVEKSSDFVLPSFRKAEVLNAIDFDSDLCISRPKSRLSWGIEFPFDPEFVTYVWF
HHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCHHHCCCCCCCCCCCHHHHHHHH
DALINYISFAGYLAEPDSGLPEFSKLWPADCEVIGKDILVPAHGVYWPAMLHAMGFSDEE
HHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHCCCCEECCCCHHHHHHHHHHCCCCCC
MPTLLVHGWWNINGEKMSKSIGNVVDPNLLAEQFGPEPVRYYLVRDITTGKDANFDADRL
CCEEEEEEEECCCHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCE
VMLYNTELANDLGNLCNRSINMTRRYCDSVISGAEAYDDEASKALRSTMDQAVTLFSKYM
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
DDNMASDALAALNAQVSACNAYIEQQQPWQLAKDEASAPRLRCVLRHLLECCAQTGYLIG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEE
CVLPDASARILGQLNAGDLFRGLTPAALKWGILPAGHRINDPAPVFPRILSEEEKAKLAE
EECCCCHHHHHCCCCHHHHHHCCCHHHHHHCCCCCCCCCCCCCCHHHHHHCHHHHHHHHH
KAARAAKKQG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]