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The map label for this gene is waaA [H]

Identifier: 187734981

GI number: 187734981

Start: 562895

End: 564181

Strand: Reverse

Name: waaA [H]

Synonym: Amuc_0474

Alternate gene names: 187734981

Gene position: 564181-562895 (Counterclockwise)

Preceding gene: 187734982

Following gene: 187734977

Centisome position: 21.18

GC content: 59.05

Gene sequence:

>1287_bases
ATGAAAGCCTTCCTGTTTTCCTTCTGTTACAATATCCTGTATACCGTCGGGTGGCTGGTAACGCTGCCTTCCTATCTGCT
TAAGCAGAAGAGGAGAGGCGGATTCGGCACGGGGCTTCTGGAGCGTTTCGGGCTGTACCGGGTCTCCTATAACCGGGAGC
CGAAAGGGGTGTTGTATGTGCATGCCGTGAGCGTGGGTGAAGTGGTGCTGGCTCTCAAGTTTCTCCGGGCGTGGCTCCGG
GAACGCGGAGGTTCCGCCGTGCTGGCTACCAGTACGGCTACGGGGCATGCTACGGCAGTGAGTGCGCAGATTCCCGGCGT
GCGCGTGATTTATGCCCCCTTTGACCTTCTGGGGCTGCCGGGGAGGTGTTTTGACCGTTTTGAACCGGAGGCCATTGTCC
TGGTGGAGGCCGAGTTGTGGCCGAATTTTGCCCGTGCAGCCAAGGTGCGCGGCATACCCATGGCGATGATTAATGCCCGT
ATGTCCGCCAGGTCCGAGAGCCGCTACCGCGCATTTAAATGGATTTCAAAGTATTATTTTTCCTTCCTTGACGCAATGGG
CGTACAGGACAAGGGGGATGTCCGGAGGTTTGAATCCGTGGGCGTGCGTTCCTCCATCATCCACGTGACGGGAAGCATCA
AATTTGACCAGCAAATGGCGGAGCGCAGGGAGGCTAATGCGGAGTTTGCCTCCATTTTGGACAAACTGAAACGCGGCAAG
CCTGTAGTGCTGGCCGCCAGCACGCATGACGGGGAGGAAGTTCTGATCGCAGAAGCGGCGCGCAAGGCGGGAGGCTTCCC
CCTGATCGTGCCCAGGCATGCGGAACGCCGCCATGCCGTGGTGCGGGAACTGGAGGCCCAGGGCTGGCAGTGCGTGCTGC
GGACTGATGGCGAGATTCCGGAAACCCTGAAGGATCATGTTTGTTACATCGCGGATACGACGGGGGAATTGAGGGACTGG
ACCGCGCTGGCGGATGTAGCCGTGATCGGAAAAAGCTTTTTGGCGGACGGTGGACAGAATCCGGCTGAAGCCGTCGCCTG
TGGCGTGCCCGTGCTGACGGGGCCCCATATGGAAAATTTCGATGCACTGGTCCAGCTGCTTGAAGGAGTGGACGGAATTG
CCAGGTGTGATGAAAACCGCCTGGCGGACGTGCTCAAGGAGATGCTGGACAACCCCCTGCTGGCTCATGCCCAGTCTTCC
CGCGCCCAGGTGGCGTTGAAGGCCCATTTCGGCGCTACGGCCCGGACCATTCGCATGATCTGCATCATGCTCAAGATTCC
TGTTTGA

Upstream 100 bases:

>100_bases
GGAAGGGAAGGCGCCGCGGAAATTGACGGCCTGAATCTGGTGGATACCCGCCGGTACGGGAAAAATATGCTGCTGTTCTA
CCAATTGCCTGAGAAGTAAG

Downstream 100 bases:

>100_bases
TATCGGGGGCTTTTTGCCCCTGGGGCTCCGGCCTGGAATGGGGAGGAAGGGCGAAGACTTCCCCCTGCGGAGCATCAATG
ATGATGTCAAAATGCTGGAA

Product: Three-deoxy-D-manno-octulosonic-acid transferase domain protein

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKAFLFSFCYNILYTVGWLVTLPSYLLKQKRRGGFGTGLLERFGLYRVSYNREPKGVLYVHAVSVGEVVLALKFLRAWLR
ERGGSAVLATSTATGHATAVSAQIPGVRVIYAPFDLLGLPGRCFDRFEPEAIVLVEAELWPNFARAAKVRGIPMAMINAR
MSARSESRYRAFKWISKYYFSFLDAMGVQDKGDVRRFESVGVRSSIIHVTGSIKFDQQMAERREANAEFASILDKLKRGK
PVVLAASTHDGEEVLIAEAARKAGGFPLIVPRHAERRHAVVRELEAQGWQCVLRTDGEIPETLKDHVCYIADTTGELRDW
TALADVAVIGKSFLADGGQNPAEAVACGVPVLTGPHMENFDALVQLLEGVDGIARCDENRLADVLKEMLDNPLLAHAQSS
RAQVALKAHFGATARTIRMICIMLKIPV

Sequences:

>Translated_428_residues
MKAFLFSFCYNILYTVGWLVTLPSYLLKQKRRGGFGTGLLERFGLYRVSYNREPKGVLYVHAVSVGEVVLALKFLRAWLR
ERGGSAVLATSTATGHATAVSAQIPGVRVIYAPFDLLGLPGRCFDRFEPEAIVLVEAELWPNFARAAKVRGIPMAMINAR
MSARSESRYRAFKWISKYYFSFLDAMGVQDKGDVRRFESVGVRSSIIHVTGSIKFDQQMAERREANAEFASILDKLKRGK
PVVLAASTHDGEEVLIAEAARKAGGFPLIVPRHAERRHAVVRELEAQGWQCVLRTDGEIPETLKDHVCYIADTTGELRDW
TALADVAVIGKSFLADGGQNPAEAVACGVPVLTGPHMENFDALVQLLEGVDGIARCDENRLADVLKEMLDNPLLAHAQSS
RAQVALKAHFGATARTIRMICIMLKIPV
>Mature_428_residues
MKAFLFSFCYNILYTVGWLVTLPSYLLKQKRRGGFGTGLLERFGLYRVSYNREPKGVLYVHAVSVGEVVLALKFLRAWLR
ERGGSAVLATSTATGHATAVSAQIPGVRVIYAPFDLLGLPGRCFDRFEPEAIVLVEAELWPNFARAAKVRGIPMAMINAR
MSARSESRYRAFKWISKYYFSFLDAMGVQDKGDVRRFESVGVRSSIIHVTGSIKFDQQMAERREANAEFASILDKLKRGK
PVVLAASTHDGEEVLIAEAARKAGGFPLIVPRHAERRHAVVRELEAQGWQCVLRTDGEIPETLKDHVCYIADTTGELRDW
TALADVAVIGKSFLADGGQNPAEAVACGVPVLTGPHMENFDALVQLLEGVDGIARCDENRLADVLKEMLDNPLLAHAQSS
RAQVALKAHFGATARTIRMICIMLKIPV

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=359, Percent_Identity=34.5403899721448, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 47233; Mature: 47233

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFLFSFCYNILYTVGWLVTLPSYLLKQKRRGGFGTGLLERFGLYRVSYNREPKGVLYV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEECCCCCCCEEEE
HAVSVGEVVLALKFLRAWLRERGGSAVLATSTATGHATAVSAQIPGVRVIYAPFDLLGLP
EECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCEEEEEECHHHHCCC
GRCFDRFEPEAIVLVEAELWPNFARAAKVRGIPMAMINARMSARSESRYRAFKWISKYYF
CHHHHCCCCCEEEEEEHHHCCCHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
SFLDAMGVQDKGDVRRFESVGVRSSIIHVTGSIKFDQQMAERREANAEFASILDKLKRGK
HHHHHCCCCCCHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHCCC
PVVLAASTHDGEEVLIAEAARKAGGFPLIVPRHAERRHAVVRELEAQGWQCVLRTDGEIP
CEEEEECCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCC
ETLKDHVCYIADTTGELRDWTALADVAVIGKSFLADGGQNPAEAVACGVPVLTGPHMENF
HHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCH
DALVQLLEGVDGIARCDENRLADVLKEMLDNPLLAHAQSSRAQVALKAHFGATARTIRMI
HHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCHHHHHHHH
CIMLKIPV
HHHHHCCC
>Mature Secondary Structure
MKAFLFSFCYNILYTVGWLVTLPSYLLKQKRRGGFGTGLLERFGLYRVSYNREPKGVLYV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEECCCCCCCEEEE
HAVSVGEVVLALKFLRAWLRERGGSAVLATSTATGHATAVSAQIPGVRVIYAPFDLLGLP
EECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCEEEEEECHHHHCCC
GRCFDRFEPEAIVLVEAELWPNFARAAKVRGIPMAMINARMSARSESRYRAFKWISKYYF
CHHHHCCCCCEEEEEEHHHCCCHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
SFLDAMGVQDKGDVRRFESVGVRSSIIHVTGSIKFDQQMAERREANAEFASILDKLKRGK
HHHHHCCCCCCHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHCCC
PVVLAASTHDGEEVLIAEAARKAGGFPLIVPRHAERRHAVVRELEAQGWQCVLRTDGEIP
CEEEEECCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCC
ETLKDHVCYIADTTGELRDWTALADVAVIGKSFLADGGQNPAEAVACGVPVLTGPHMENF
HHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCH
DALVQLLEGVDGIARCDENRLADVLKEMLDNPLLAHAQSSRAQVALKAHFGATARTIRMI
HHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCHHHHHHHH
CIMLKIPV
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]