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The map label for this gene is nifS1 [H]

Identifier: 187734977

GI number: 187734977

Start: 558748

End: 559902

Strand: Reverse

Name: nifS1 [H]

Synonym: Amuc_0470

Alternate gene names: 187734977

Gene position: 559902-558748 (Counterclockwise)

Preceding gene: 187734981

Following gene: 187734976

Centisome position: 21.02

GC content: 59.22

Gene sequence:

>1155_bases
GTGATTTATTGGGATAATAACGCCACAACACCGCTGGCTCCGGAGGTTTACGAGGCCATGGAGCCGTTTTTAAAGGAACG
GTTTTTCAACCCGTCCGCCGGTTATGGCTGTGCCAGGCATGTGCGTGAGGCGGTGGAGGAGGCCCGTGCGGCCGTTGCCG
CTCTGTTGGGCGCATTTCCTTCGGAAATTGTATTTACTTCCGGCGGAACGGAAGCGTCCAATATGGCTTTCCGCCAGATG
GCCGGGGGACAGGAGGGGGGGATAGGCGTTTTGTCCACGGACCATGACGCCTCCTTGAAGACGGCCCTGGCCCTGGGCTA
CGGCTGTGTCTGCCCCGTGGACAGGGAAGGGAGAGCGATTCCGGAAAAATGGGAGTCGATGTGCGCAGGGGGCGTATCCG
GCGTTTCTTTTGCCTGGGCCAATAATGAGACAGGCGCATTGCAGGATGCGCCGGTTTTGTGTTCCTCCGCCAGGAAGCAT
GGAGTTCCCGTGCATTTGGATATAGTGCAGTGCGCCGGAAAAATTCCCGTGGATTTGCATGGAATGGAAGTGGATTATGC
TTCCGTATCCGCACACAAATTCCATGGCCCCAAGGGGATTGGATGTCTGTACAGGAGAGCGGGGGCTCCTTTTTCCCCTT
TGTTGTTCGGCGGGGGGCAGGAGTACGGCTTGAGGTCCGGCACGGAGAATGTTCCGGGAATCATTGGTTTTGGTGCGGCG
GCGCGGGCGGCATTGAGGCACTTGGAGGAGGACGCCGGACGGCTGTCCCGGATGCGGGATTCTTTTGAGTCTCTCCTGCG
GACACAGGTGGGCGGCGTGACGGTGCATTCCGGCGGAACGGAACGTATTCCCAATACTTCTAATCTGGCGTTTGACGGAT
GTACGGCGGAAGCGCTGATGCTGTTGCTGGAACCTGCTGGTCTGCTGTGTTCGGCAGGGTCTGCCTGCCATACGCTGCAG
CCGATGCCTTCCCACGTGCTGACGGCCATGGGATTATCTGATGAGGCGGTACGTTCCTCCCTGCGTTTTTCCCTGTCTTT
CATGACGACGCAGGAAGAGGTGGAGCAGGCTGCAGCCCTGGTGGGCTCCGCTGTTCGGAAGGTGCGCTGTGTCCAGTCTT
CGCGTACCGGCCCGGTGCTTGTGTACAGGCCGTAG

Upstream 100 bases:

>100_bases
CATTATGCGGAATCACGGGTAAAAGTGAAGAGTGATCTTGTGCAGCTGCGCATTATGTTCCGGCTTGACTCCTTCCCGCG
ATTGGGGGAGTTATGAAGGC

Downstream 100 bases:

>100_bases
TTGAAGGGTGGAAAGGCTGGTAGCTCTTTCAGATGGCCTTTTGTGTTCCATGATCCGGGGGACAGTGATGTGGTGAGGGG
GAAAGGGTGGAAAAAAGCTG

Product: aminotransferase class V

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MIYWDNNATTPLAPEVYEAMEPFLKERFFNPSAGYGCARHVREAVEEARAAVAALLGAFPSEIVFTSGGTEASNMAFRQM
AGGQEGGIGVLSTDHDASLKTALALGYGCVCPVDREGRAIPEKWESMCAGGVSGVSFAWANNETGALQDAPVLCSSARKH
GVPVHLDIVQCAGKIPVDLHGMEVDYASVSAHKFHGPKGIGCLYRRAGAPFSPLLFGGGQEYGLRSGTENVPGIIGFGAA
ARAALRHLEEDAGRLSRMRDSFESLLRTQVGGVTVHSGGTERIPNTSNLAFDGCTAEALMLLLEPAGLLCSAGSACHTLQ
PMPSHVLTAMGLSDEAVRSSLRFSLSFMTTQEEVEQAAALVGSAVRKVRCVQSSRTGPVLVYRP

Sequences:

>Translated_384_residues
MIYWDNNATTPLAPEVYEAMEPFLKERFFNPSAGYGCARHVREAVEEARAAVAALLGAFPSEIVFTSGGTEASNMAFRQM
AGGQEGGIGVLSTDHDASLKTALALGYGCVCPVDREGRAIPEKWESMCAGGVSGVSFAWANNETGALQDAPVLCSSARKH
GVPVHLDIVQCAGKIPVDLHGMEVDYASVSAHKFHGPKGIGCLYRRAGAPFSPLLFGGGQEYGLRSGTENVPGIIGFGAA
ARAALRHLEEDAGRLSRMRDSFESLLRTQVGGVTVHSGGTERIPNTSNLAFDGCTAEALMLLLEPAGLLCSAGSACHTLQ
PMPSHVLTAMGLSDEAVRSSLRFSLSFMTTQEEVEQAAALVGSAVRKVRCVQSSRTGPVLVYRP
>Mature_384_residues
MIYWDNNATTPLAPEVYEAMEPFLKERFFNPSAGYGCARHVREAVEEARAAVAALLGAFPSEIVFTSGGTEASNMAFRQM
AGGQEGGIGVLSTDHDASLKTALALGYGCVCPVDREGRAIPEKWESMCAGGVSGVSFAWANNETGALQDAPVLCSSARKH
GVPVHLDIVQCAGKIPVDLHGMEVDYASVSAHKFHGPKGIGCLYRRAGAPFSPLLFGGGQEYGLRSGTENVPGIIGFGAA
ARAALRHLEEDAGRLSRMRDSFESLLRTQVGGVTVHSGGTERIPNTSNLAFDGCTAEALMLLLEPAGLLCSAGSACHTLQ
PMPSHVLTAMGLSDEAVRSSLRFSLSFMTTQEEVEQAAALVGSAVRKVRCVQSSRTGPVLVYRP

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=383, Percent_Identity=34.7258485639687, Blast_Score=204, Evalue=9e-53,
Organism=Homo sapiens, GI156713448, Length=412, Percent_Identity=32.5242718446602, Blast_Score=190, Evalue=2e-48,
Organism=Escherichia coli, GI48994898, Length=381, Percent_Identity=36.4829396325459, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI1789175, Length=409, Percent_Identity=27.6283618581907, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25143064, Length=381, Percent_Identity=34.9081364829396, Blast_Score=204, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI17533177, Length=311, Percent_Identity=30.2250803858521, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319831, Length=379, Percent_Identity=35.3562005277045, Blast_Score=202, Evalue=5e-53,
Organism=Drosophila melanogaster, GI20129463, Length=378, Percent_Identity=34.9206349206349, Blast_Score=202, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 40518; Mature: 40518

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYWDNNATTPLAPEVYEAMEPFLKERFFNPSAGYGCARHVREAVEEARAAVAALLGAFP
CEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
SEIVFTSGGTEASNMAFRQMAGGQEGGIGVLSTDHDASLKTALALGYGCVCPVDREGRAI
CCEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCC
PEKWESMCAGGVSGVSFAWANNETGALQDAPVLCSSARKHGVPVHLDIVQCAGKIPVDLH
CHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEHHHHCCCCCEEEE
GMEVDYASVSAHKFHGPKGIGCLYRRAGAPFSPLLFGGGQEYGLRSGTENVPGIIGFGAA
CCEECHHHCCHHHCCCCCCHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCEEECCHH
ARAALRHLEEDAGRLSRMRDSFESLLRTQVGGVTVHSGGTERIPNTSNLAFDGCTAEALM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCEECCHHHHHHH
LLLEPAGLLCSAGSACHTLQPMPSHVLTAMGLSDEAVRSSLRFSLSFMTTQEEVEQAAAL
HHHCCCCHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
VGSAVRKVRCVQSSRTGPVLVYRP
HHHHHHHHHHHHCCCCCCEEEECC
>Mature Secondary Structure
MIYWDNNATTPLAPEVYEAMEPFLKERFFNPSAGYGCARHVREAVEEARAAVAALLGAFP
CEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
SEIVFTSGGTEASNMAFRQMAGGQEGGIGVLSTDHDASLKTALALGYGCVCPVDREGRAI
CCEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCC
PEKWESMCAGGVSGVSFAWANNETGALQDAPVLCSSARKHGVPVHLDIVQCAGKIPVDLH
CHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEHHHHCCCCCEEEE
GMEVDYASVSAHKFHGPKGIGCLYRRAGAPFSPLLFGGGQEYGLRSGTENVPGIIGFGAA
CCEECHHHCCHHHCCCCCCHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCEEECCHH
ARAALRHLEEDAGRLSRMRDSFESLLRTQVGGVTVHSGGTERIPNTSNLAFDGCTAEALM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCEECCHHHHHHH
LLLEPAGLLCSAGSACHTLQPMPSHVLTAMGLSDEAVRSSLRFSLSFMTTQEEVEQAAAL
HHHCCCCHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
VGSAVRKVRCVQSSRTGPVLVYRP
HHHHHHHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]