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The map label for this gene is rsmD [H]

Identifier: 187734982

GI number: 187734982

Start: 564183

End: 564758

Strand: Reverse

Name: rsmD [H]

Synonym: Amuc_0475

Alternate gene names: 187734982

Gene position: 564758-564183 (Counterclockwise)

Preceding gene: 187734983

Following gene: 187734981

Centisome position: 21.2

GC content: 59.38

Gene sequence:

>576_bases
ATGCGTATCATCAGCGGAAAGGCCGGCGGCATAACCCTGTCTGTTCCCAAGGGGGAAGTTCGTCCCACGACAGACCGGGT
CAGGGAAGCCGTTTTTTCCATCCTGAACCCGCTGATGGACCGGGCCGACGTGCTGGATTTATTTGCCGGGTCCGGCGCCT
TCGGCCTGGAGGCCCTCAGCCGTGGGGCGAGAGACGCCCGCATGGTGGATTTTTCCCGTCTCTCCTGCGCGGCGGCCAGG
GCTAATCTCGTCAAGACGGGCTTGGAAGGCGGAACTGTCATCCAGGGAGATGCCGTCCAGTTCGTGAGAAGGGAACTGCT
GGCCGGCAGGAAGTACGATATTATTTTTGCAGATCCTCCTTATTGCAAGGGGCCGGCGGACCGCGACTTTATTGTTGAAC
TGGCGGAGGCCGGAGTTGCCGGACTTTTGAAGGGCGGTGGCGTGTTTATTGCGGAAGTGCAGGAGGGCTGGGGTACCGGA
AGGGAAGGCGCCGCGGAAATTGACGGCCTGAATCTGGTGGATACCCGCCGGTACGGGAAAAATATGCTGCTGTTCTACCA
ATTGCCTGAGAAGTAA

Upstream 100 bases:

>100_bases
TCCCGTCACCCGCATCGCGGGTGACCCTTTGCTGGAAAAGGCTAAGGCCAAGGCTCGCCAGTAGGGATATGATGTTCCTT
TCATTTCCGGTAAGACAACA

Downstream 100 bases:

>100_bases
GATGAAAGCCTTCCTGTTTTCCTTCTGTTACAATATCCTGTATACCGTCGGGTGGCTGGTAACGCTGCCTTCCTATCTGC
TTAAGCAGAAGAGGAGAGGC

Product: methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MRIISGKAGGITLSVPKGEVRPTTDRVREAVFSILNPLMDRADVLDLFAGSGAFGLEALSRGARDARMVDFSRLSCAAAR
ANLVKTGLEGGTVIQGDAVQFVRRELLAGRKYDIIFADPPYCKGPADRDFIVELAEAGVAGLLKGGGVFIAEVQEGWGTG
REGAAEIDGLNLVDTRRYGKNMLLFYQLPEK

Sequences:

>Translated_191_residues
MRIISGKAGGITLSVPKGEVRPTTDRVREAVFSILNPLMDRADVLDLFAGSGAFGLEALSRGARDARMVDFSRLSCAAAR
ANLVKTGLEGGTVIQGDAVQFVRRELLAGRKYDIIFADPPYCKGPADRDFIVELAEAGVAGLLKGGGVFIAEVQEGWGTG
REGAAEIDGLNLVDTRRYGKNMLLFYQLPEK
>Mature_191_residues
MRIISGKAGGITLSVPKGEVRPTTDRVREAVFSILNPLMDRADVLDLFAGSGAFGLEALSRGARDARMVDFSRLSCAAAR
ANLVKTGLEGGTVIQGDAVQFVRRELLAGRKYDIIFADPPYCKGPADRDFIVELAEAGVAGLLKGGGVFIAEVQEGWGTG
REGAAEIDGLNLVDTRRYGKNMLLFYQLPEK

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=125, Percent_Identity=40.8, Blast_Score=89, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 20477; Mature: 20477

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIISGKAGGITLSVPKGEVRPTTDRVREAVFSILNPLMDRADVLDLFAGSGAFGLEALS
CEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RGARDARMVDFSRLSCAAARANLVKTGLEGGTVIQGDAVQFVRRELLAGRKYDIIFADPP
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHCCCCEEEEECCCC
YCKGPADRDFIVELAEAGVAGLLKGGGVFIAEVQEGWGTGREGAAEIDGLNLVDTRRYGK
CCCCCCCHHHHHHHHHCCHHHEECCCCEEEEEECCCCCCCCCCCCCCCCCCEEHHHHCCC
NMLLFYQLPEK
CEEEEEECCCC
>Mature Secondary Structure
MRIISGKAGGITLSVPKGEVRPTTDRVREAVFSILNPLMDRADVLDLFAGSGAFGLEALS
CEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RGARDARMVDFSRLSCAAARANLVKTGLEGGTVIQGDAVQFVRRELLAGRKYDIIFADPP
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHCCCCEEEEECCCC
YCKGPADRDFIVELAEAGVAGLLKGGGVFIAEVQEGWGTGREGAAEIDGLNLVDTRRYGK
CCCCCCCHHHHHHHHHCCHHHEECCCCEEEEEECCCCCCCCCCCCCCCCCCEEHHHHCCC
NMLLFYQLPEK
CEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]