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The map label for this gene is dnaB [H]

Identifier: 187734934

GI number: 187734934

Start: 510517

End: 511971

Strand: Direct

Name: dnaB [H]

Synonym: Amuc_0427

Alternate gene names: 187734934

Gene position: 510517-511971 (Clockwise)

Preceding gene: 187734933

Following gene: 187734935

Centisome position: 19.16

GC content: 56.43

Gene sequence:

>1455_bases
ATGGAAACCACGACAACCACCACTGCCGTTCCCCCGCTGGAACTGACCAGGGCACCCCGCAAAACCCCGTCCCTGGACCT
CAGAGACATTCCGCAGGCTCCCGGCCCGGAAAAAGGAGTGCTGGCCCTCATGGCCATGGACCCGGCCACTTACGTGGGGC
AGTGCGTGACCATCGGCATGACGGAGGACTACTTCTACCTCCCCGCGCACAAGCTGCTCTGGCGCCTTTTCCAGGCCCGC
TATAACAAAAACGAACCCATTGACATCGTTTCCATCACCCAAGCTTTGGAAGACATGCACCAGCTGGAAGCCGTGGGCGG
AAGCGCGGGCCTGGCGGAAATATACACCTTTACCACTACCGGAGCCTATTTTGAGCATTACCTCAATGTCCTGAAGGACA
AATTCATCCTTCGCTCCATCATTGACATAGCCAACCAGTCCACCACGCAGGCCTTTGACAATCCGGACGACGTAGCGGAA
CTGCTGGATTCTGTGGAAACCCATATTTTCCAGATACGGGAACGCTATAACAGCGCCAAGGATGAACAAAGCCTGGCCAG
CATCCTTAAACAAGCCGTCACCAATTTTGAAAAATTCATCGCAAGCAAAGGCCAAATCCAGGGACTGACCACGGGATTTG
AGGAACTGGACAAAAAAAGCAACGGGCTTAAACCGGGGGACATGTTCATCATCGCCGCCCGCCCCTCCATGGGTAAAACC
TCCTTTCTGCTCAACATCATTGAGCACATCGCGCTTAACGAAAAAAAGCCTACTCTGCTCTTTTCCTGTGAAATGCCCGC
AGTCCAAATTGTGGAACGCCTTCTTTTTGCCCGCTCCGGAGTCCGCAGCCGGGAAATCATCAAGAGAGGCAACCTCACCC
AGCTTGAAATGAAGCATTTCAAGCAAGCGGTCAAGGAAGTGGGAGCTTCCCAGCTTGTCATTGACGATACCGCAGCCATC
TCCATCAACGAACTCCGGGCCAAAGCGCGCCGTGTCATGAGGGATCAGGGAGGTCTGGCCGCCATTGGTGTGGACTATCT
CCAGCTCATGCGCTCCCACTCCAAACAGGCGGCCAACAGTCGTGAACGGGAAGTGGCGGAAATATCCGCCGGCCTGAAAG
CCCTGGCCAAGGAACTCAAAGTGCCCGTCATCGTTCTGGCCCAGCTCAACCGCGGCCCGGAAAGCCGCACGGGAGCCAGC
CTGGGCGTCCCCCGCATCTCCGACCTTCGTGAATCCGGCTCCATCGAACAGGACGCAGACATGATCGGCCTGCTCTACCG
CTCCGCCTACTATGCGGAAGATGAGGAAAAACGCCAGCAAATGGCCGGACGGGCCAATCTGCATCTGGCCAAAAACCGCA
ACGGCCCCACGGGGGACGTTCCGCTCCACTTTGAAGCGGAACTCATGCGCTTCTCCACACGTGAGGCAGATGAACACGAC
CAGGAAAGCGAATAA

Upstream 100 bases:

>100_bases
CGGAACGGAGCTTTTCCGAATTTACAACGTTGCCTATTGCCCGGCGCCCTCCGTTGTGCTATCCTTTCCGGCCGGATGCC
ACCCATCCAGCCGCCTCACC

Downstream 100 bases:

>100_bases
AACCTCTCGCTGTCTGTGCCTGTTCTCTGCGGATTAACAATCCGTTACAGGCTGAAACCGAAGATAATTCTGTTTAATTC
GGGCAAAGTTACAAGTTTCT

Product: DnaB domain protein helicase domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
METTTTTTAVPPLELTRAPRKTPSLDLRDIPQAPGPEKGVLALMAMDPATYVGQCVTIGMTEDYFYLPAHKLLWRLFQAR
YNKNEPIDIVSITQALEDMHQLEAVGGSAGLAEIYTFTTTGAYFEHYLNVLKDKFILRSIIDIANQSTTQAFDNPDDVAE
LLDSVETHIFQIRERYNSAKDEQSLASILKQAVTNFEKFIASKGQIQGLTTGFEELDKKSNGLKPGDMFIIAARPSMGKT
SFLLNIIEHIALNEKKPTLLFSCEMPAVQIVERLLFARSGVRSREIIKRGNLTQLEMKHFKQAVKEVGASQLVIDDTAAI
SINELRAKARRVMRDQGGLAAIGVDYLQLMRSHSKQAANSREREVAEISAGLKALAKELKVPVIVLAQLNRGPESRTGAS
LGVPRISDLRESGSIEQDADMIGLLYRSAYYAEDEEKRQQMAGRANLHLAKNRNGPTGDVPLHFEAELMRFSTREADEHD
QESE

Sequences:

>Translated_484_residues
METTTTTTAVPPLELTRAPRKTPSLDLRDIPQAPGPEKGVLALMAMDPATYVGQCVTIGMTEDYFYLPAHKLLWRLFQAR
YNKNEPIDIVSITQALEDMHQLEAVGGSAGLAEIYTFTTTGAYFEHYLNVLKDKFILRSIIDIANQSTTQAFDNPDDVAE
LLDSVETHIFQIRERYNSAKDEQSLASILKQAVTNFEKFIASKGQIQGLTTGFEELDKKSNGLKPGDMFIIAARPSMGKT
SFLLNIIEHIALNEKKPTLLFSCEMPAVQIVERLLFARSGVRSREIIKRGNLTQLEMKHFKQAVKEVGASQLVIDDTAAI
SINELRAKARRVMRDQGGLAAIGVDYLQLMRSHSKQAANSREREVAEISAGLKALAKELKVPVIVLAQLNRGPESRTGAS
LGVPRISDLRESGSIEQDADMIGLLYRSAYYAEDEEKRQQMAGRANLHLAKNRNGPTGDVPLHFEAELMRFSTREADEHD
QESE
>Mature_484_residues
METTTTTTAVPPLELTRAPRKTPSLDLRDIPQAPGPEKGVLALMAMDPATYVGQCVTIGMTEDYFYLPAHKLLWRLFQAR
YNKNEPIDIVSITQALEDMHQLEAVGGSAGLAEIYTFTTTGAYFEHYLNVLKDKFILRSIIDIANQSTTQAFDNPDDVAE
LLDSVETHIFQIRERYNSAKDEQSLASILKQAVTNFEKFIASKGQIQGLTTGFEELDKKSNGLKPGDMFIIAARPSMGKT
SFLLNIIEHIALNEKKPTLLFSCEMPAVQIVERLLFARSGVRSREIIKRGNLTQLEMKHFKQAVKEVGASQLVIDDTAAI
SINELRAKARRVMRDQGGLAAIGVDYLQLMRSHSKQAANSREREVAEISAGLKALAKELKVPVIVLAQLNRGPESRTGAS
LGVPRISDLRESGSIEQDADMIGLLYRSAYYAEDEEKRQQMAGRANLHLAKNRNGPTGDVPLHFEAELMRFSTREADEHD
QESE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=459, Percent_Identity=36.6013071895425, Blast_Score=278, Evalue=5e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 53836; Mature: 53836

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METTTTTTAVPPLELTRAPRKTPSLDLRDIPQAPGPEKGVLALMAMDPATYVGQCVTIGM
CCCCCCCCCCCCHHHHCCCCCCCCCCHHCCCCCCCCCCCEEEEEEECCHHHHCCEEEEEC
TEDYFYLPAHKLLWRLFQARYNKNEPIDIVSITQALEDMHQLEAVGGSAGLAEIYTFTTT
CCCEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEHHH
GAYFEHYLNVLKDKFILRSIIDIANQSTTQAFDNPDDVAELLDSVETHIFQIRERYNSAK
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DEQSLASILKQAVTNFEKFIASKGQIQGLTTGFEELDKKSNGLKPGDMFIIAARPSMGKT
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHH
SFLLNIIEHIALNEKKPTLLFSCEMPAVQIVERLLFARSGVRSREIIKRGNLTQLEMKHF
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHH
KQAVKEVGASQLVIDDTAAISINELRAKARRVMRDQGGLAAIGVDYLQLMRSHSKQAANS
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHH
REREVAEISAGLKALAKELKVPVIVLAQLNRGPESRTGASLGVPRISDLRESGSIEQDAD
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHH
MIGLLYRSAYYAEDEEKRQQMAGRANLHLAKNRNGPTGDVPLHFEAELMRFSTREADEHD
HHHHHHHHHHCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHCCCCCCC
QESE
CCCC
>Mature Secondary Structure
METTTTTTAVPPLELTRAPRKTPSLDLRDIPQAPGPEKGVLALMAMDPATYVGQCVTIGM
CCCCCCCCCCCCHHHHCCCCCCCCCCHHCCCCCCCCCCCEEEEEEECCHHHHCCEEEEEC
TEDYFYLPAHKLLWRLFQARYNKNEPIDIVSITQALEDMHQLEAVGGSAGLAEIYTFTTT
CCCEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEHHH
GAYFEHYLNVLKDKFILRSIIDIANQSTTQAFDNPDDVAELLDSVETHIFQIRERYNSAK
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DEQSLASILKQAVTNFEKFIASKGQIQGLTTGFEELDKKSNGLKPGDMFIIAARPSMGKT
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHH
SFLLNIIEHIALNEKKPTLLFSCEMPAVQIVERLLFARSGVRSREIIKRGNLTQLEMKHF
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHH
KQAVKEVGASQLVIDDTAAISINELRAKARRVMRDQGGLAAIGVDYLQLMRSHSKQAANS
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHH
REREVAEISAGLKALAKELKVPVIVLAQLNRGPESRTGASLGVPRISDLRESGSIEQDAD
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHH
MIGLLYRSAYYAEDEEKRQQMAGRANLHLAKNRNGPTGDVPLHFEAELMRFSTREADEHD
HHHHHHHHHHCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHCCCCCCC
QESE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]