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The map label for this gene is rlmN

Identifier: 187734933

GI number: 187734933

Start: 509337

End: 510416

Strand: Direct

Name: rlmN

Synonym: Amuc_0426

Alternate gene names: 187734933

Gene position: 509337-510416 (Clockwise)

Preceding gene: 187734932

Following gene: 187734934

Centisome position: 19.12

GC content: 56.94

Gene sequence:

>1080_bases
ATGCCACCTCTTCCCCTCATCACTGCCCAGACGGAGGAAAAGCTGCTCGCCTTCCTGACGGAACACGGGCACACTAAATT
CCGCACCCAGCAGGTTCTGGACTGGGTGTGGCGCAAGCGCGTCACCACCTTTGACGCGATGAGCAACCTCCCTCCCGCGC
TCAAAAATCTGCTGGCGGAAAATTTCCGTTTCCACACGCCGGAAATCGTGGAAATCCACGGTTCTGCGGACACCACACGA
AAATTCCTCACCAAAATGGAAGACGGCAGTCTGGTAGAATCCGTCATCATCCCTGCCGCAGCAGCGGAAAATGGAGAAAA
ATCGGAGCGCGTCACCCTTTGCGTTTCTTCCCAGGTAGGCTGCGCCTTCGGCTGCAAATTCTGCGCTTCCGGGCTGCTGG
GCCTTAAACGCCACCTCACCACGGGGGAAATCATCGGGCAGATTCTCTCCGCGGAAGCCATTGCGGGAAAACGGGTCAAT
AACATCGTCTTCATGGGCATGGGGGAACCCCTCTCCAATTTTGACAACCTGGCGGATGCCCTGGAAATCATCACTTCCCA
CCGCGGTCTGGAAATAGGGGCGCGCCACATCACCATCTCCACGTCGGGATTCGTTCCCGGCCTGAAAAAACTGGCGGCCT
ATCCCAGGCAAATCCGTCTGGCCGTTTCTCTGCACGGAGCTACGGACGAAGTTCGCGACCAAATCATGCCGGTCAACAAA
AAATGGCCGCTCTCCCAGCTCATTCCGGCCCTTGAGGAATGGAACAGGGGAAGAAACCAGATGCCCACGCTGGAATACAT
TCTCATCAGGGACATCAACGATTCTCCAAAAGACGCGTCCCATCTCGTCCGGATCGCCAAACGCCTGCACGCCAAAGTCA
ATCTCATCCCGTACAACACCGTGGAGGGGCTCCCGTGGAAACGCCCTTCGGAAGAACGCTGCCGTTCCTTCCGGGATGCA
GTTCACAAGGCGCGCATCCCCGTTACCATGCGGTATGAAAAAGGCCATGACATCAACGCCGCCTGCGGCCAGTTGAGGCT
GAGAAAGGAACAGGAAAAGAGCGGAAAGACGTCCCGTTGA

Upstream 100 bases:

>100_bases
CCCCTCCTTTCTGGGAACCGGGGAAGGAGCCGAAGACTTTGAACCCTTCAACCGGGACCGCTACGTGGATTCCCTGCTGT
AACGGGACCTCCTCAAATCC

Downstream 100 bases:

>100_bases
CGGAACGGAGCTTTTCCGAATTTACAACGTTGCCTATTGCCCGGCGCCCTCCGTTGTGCTATCCTTTCCGGCCGGATGCC
ACCCATCCAGCCGCCTCACC

Product: radical SAM enzyme, Cfr family

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MPPLPLITAQTEEKLLAFLTEHGHTKFRTQQVLDWVWRKRVTTFDAMSNLPPALKNLLAENFRFHTPEIVEIHGSADTTR
KFLTKMEDGSLVESVIIPAAAAENGEKSERVTLCVSSQVGCAFGCKFCASGLLGLKRHLTTGEIIGQILSAEAIAGKRVN
NIVFMGMGEPLSNFDNLADALEIITSHRGLEIGARHITISTSGFVPGLKKLAAYPRQIRLAVSLHGATDEVRDQIMPVNK
KWPLSQLIPALEEWNRGRNQMPTLEYILIRDINDSPKDASHLVRIAKRLHAKVNLIPYNTVEGLPWKRPSEERCRSFRDA
VHKARIPVTMRYEKGHDINAACGQLRLRKEQEKSGKTSR

Sequences:

>Translated_359_residues
MPPLPLITAQTEEKLLAFLTEHGHTKFRTQQVLDWVWRKRVTTFDAMSNLPPALKNLLAENFRFHTPEIVEIHGSADTTR
KFLTKMEDGSLVESVIIPAAAAENGEKSERVTLCVSSQVGCAFGCKFCASGLLGLKRHLTTGEIIGQILSAEAIAGKRVN
NIVFMGMGEPLSNFDNLADALEIITSHRGLEIGARHITISTSGFVPGLKKLAAYPRQIRLAVSLHGATDEVRDQIMPVNK
KWPLSQLIPALEEWNRGRNQMPTLEYILIRDINDSPKDASHLVRIAKRLHAKVNLIPYNTVEGLPWKRPSEERCRSFRDA
VHKARIPVTMRYEKGHDINAACGQLRLRKEQEKSGKTSR
>Mature_358_residues
PPLPLITAQTEEKLLAFLTEHGHTKFRTQQVLDWVWRKRVTTFDAMSNLPPALKNLLAENFRFHTPEIVEIHGSADTTRK
FLTKMEDGSLVESVIIPAAAAENGEKSERVTLCVSSQVGCAFGCKFCASGLLGLKRHLTTGEIIGQILSAEAIAGKRVNN
IVFMGMGEPLSNFDNLADALEIITSHRGLEIGARHITISTSGFVPGLKKLAAYPRQIRLAVSLHGATDEVRDQIMPVNKK
WPLSQLIPALEEWNRGRNQMPTLEYILIRDINDSPKDASHLVRIAKRLHAKVNLIPYNTVEGLPWKRPSEERCRSFRDAV
HKARIPVTMRYEKGHDINAACGQLRLRKEQEKSGKTSR

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=346, Percent_Identity=37.2832369942197, Blast_Score=233, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_AKKM8 (B2UNF2)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877045.1
- GeneID:   6275581
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_0426
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   CLSK2335923
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 40163; Mature: 40032

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPLPLITAQTEEKLLAFLTEHGHTKFRTQQVLDWVWRKRVTTFDAMSNLPPALKNLLAE
CCCCCEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
NFRFHTPEIVEIHGSADTTRKFLTKMEDGSLVESVIIPAAAAENGEKSERVTLCVSSQVG
HCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
CAFGCKFCASGLLGLKRHLTTGEIIGQILSAEAIAGKRVNNIVFMGMGEPLSNFDNLADA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHH
LEIITSHRGLEIGARHITISTSGFVPGLKKLAAYPRQIRLAVSLHGATDEVRDQIMPVNK
HHHHHHCCCCEECCEEEEEECCCCCHHHHHHHCCCHHEEEEEEECCCCHHHHHHHCCCCC
KWPLSQLIPALEEWNRGRNQMPTLEYILIRDINDSPKDASHLVRIAKRLHAKVNLIPYNT
CCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHEEEEEECCC
VEGLPWKRPSEERCRSFRDAVHKARIPVTMRYEKGHDINAACGQLRLRKEQEKSGKTSR
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PPLPLITAQTEEKLLAFLTEHGHTKFRTQQVLDWVWRKRVTTFDAMSNLPPALKNLLAE
CCCCEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
NFRFHTPEIVEIHGSADTTRKFLTKMEDGSLVESVIIPAAAAENGEKSERVTLCVSSQVG
HCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
CAFGCKFCASGLLGLKRHLTTGEIIGQILSAEAIAGKRVNNIVFMGMGEPLSNFDNLADA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHH
LEIITSHRGLEIGARHITISTSGFVPGLKKLAAYPRQIRLAVSLHGATDEVRDQIMPVNK
HHHHHHCCCCEECCEEEEEECCCCCHHHHHHHCCCHHEEEEEEECCCCHHHHHHHCCCCC
KWPLSQLIPALEEWNRGRNQMPTLEYILIRDINDSPKDASHLVRIAKRLHAKVNLIPYNT
CCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHEEEEEECCC
VEGLPWKRPSEERCRSFRDAVHKARIPVTMRYEKGHDINAACGQLRLRKEQEKSGKTSR
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA