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The map label for this gene is panB [H]

Identifier: 187734767

GI number: 187734767

Start: 312720

End: 313508

Strand: Reverse

Name: panB [H]

Synonym: Amuc_0255

Alternate gene names: 187734767

Gene position: 313508-312720 (Counterclockwise)

Preceding gene: 187734768

Following gene: 187734766

Centisome position: 11.77

GC content: 53.99

Gene sequence:

>789_bases
ATGAATCAATCTTTTGAGAAAGCGGAACGAATCCGCGCCTGCAAGAACAGGCGGCATGTGACTCTGGTTACTGCTTATGA
CTATCCTACAGGCCGTCTTTTGGATGAGGCCGGCGTAGATATTGTGCTGGTAGGGGATTCCTTGGGGATGGTGGTTTTGG
GCTTTCCGGATACCACTCATGTGACGCTGGATCATATGCTTCATCATGTGGAAGCTACTGCGCGCGGAGTGAAGAACGCC
ATGCTGGTGGGAGATATGCCCATACATTCTTATGATACGGTGGAACAGGCCGTGCAATCCGCAAAGAAATTGTTTTTGGC
TGGAGCGGATGCTGTCAAGCTGGAAGGGGGGGCAGCCCAGGCAGGAAATATCCGTGCTATTGTGGAGGCCGGTATTCCCG
TAGTGGGTCATATCGGCCTCCTGCCGCAGAAAGTGCTGGAGGAGGGAGGATATAAGATCAAAGGGAAATCCTCCGCGGAG
GAGGAAGCCTTGGTAAGGGATGTGGAGGCTGTGGCAGAGGCGGGGGCTTGTGCTGTGGTTGTGGAAGGCGTAACGCCTCA
TGCGGCCCGACAAGTTACCGTACATTCTTCCATTCCCACGTTGGGTATTGGGTCCGGCTCCCATACATGTGATGGCGATG
TTGTGGTGATTCATGATCTGGTGGGCGCATTTCCGTGGTTTATTCCGGCTTTCGTCAAACCTCGTGCGGATGTCGCTGGA
AGCATGACTGCCGCTGTCAAGGAGTGGATGGAAGATTGCTACACGGAACCCGGCTCCATCAGCTCATAA

Upstream 100 bases:

>100_bases
GCATAAAACGGTTGCCTGCCTGTTGGCGGAATTGGATACGGATGAGGCTGATCCGCTCCTGGTATCGGAACGCTGGTAAA
TTGTATGTTTATTGAACGCC

Downstream 100 bases:

>100_bases
CAATTGATTTTTTTATGCTGGTATTTTCTACGTGCTGGAATTCCCACCGTCATCAGGACGGGGAAGAAATGATTGATGAG
ATTCTTTCTTTGGGTTTTGA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MNQSFEKAERIRACKNRRHVTLVTAYDYPTGRLLDEAGVDIVLVGDSLGMVVLGFPDTTHVTLDHMLHHVEATARGVKNA
MLVGDMPIHSYDTVEQAVQSAKKLFLAGADAVKLEGGAAQAGNIRAIVEAGIPVVGHIGLLPQKVLEEGGYKIKGKSSAE
EEALVRDVEAVAEAGACAVVVEGVTPHAARQVTVHSSIPTLGIGSGSHTCDGDVVVIHDLVGAFPWFIPAFVKPRADVAG
SMTAAVKEWMEDCYTEPGSISS

Sequences:

>Translated_262_residues
MNQSFEKAERIRACKNRRHVTLVTAYDYPTGRLLDEAGVDIVLVGDSLGMVVLGFPDTTHVTLDHMLHHVEATARGVKNA
MLVGDMPIHSYDTVEQAVQSAKKLFLAGADAVKLEGGAAQAGNIRAIVEAGIPVVGHIGLLPQKVLEEGGYKIKGKSSAE
EEALVRDVEAVAEAGACAVVVEGVTPHAARQVTVHSSIPTLGIGSGSHTCDGDVVVIHDLVGAFPWFIPAFVKPRADVAG
SMTAAVKEWMEDCYTEPGSISS
>Mature_262_residues
MNQSFEKAERIRACKNRRHVTLVTAYDYPTGRLLDEAGVDIVLVGDSLGMVVLGFPDTTHVTLDHMLHHVEATARGVKNA
MLVGDMPIHSYDTVEQAVQSAKKLFLAGADAVKLEGGAAQAGNIRAIVEAGIPVVGHIGLLPQKVLEEGGYKIKGKSSAE
EEALVRDVEAVAEAGACAVVVEGVTPHAARQVTVHSSIPTLGIGSGSHTCDGDVVVIHDLVGAFPWFIPAFVKPRADVAG
SMTAAVKEWMEDCYTEPGSISS

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate [H]

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family [H]

Homologues:

Organism=Escherichia coli, GI1786326, Length=238, Percent_Identity=37.8151260504202, Blast_Score=167, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6319653, Length=257, Percent_Identity=31.9066147859922, Blast_Score=132, Evalue=7e-32,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003700
- InterPro:   IPR015813 [H]

Pfam domain/function: PF02548 Pantoate_transf [H]

EC number: =2.1.2.11 [H]

Molecular weight: Translated: 27644; Mature: 27644

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSFEKAERIRACKNRRHVTLVTAYDYPTGRLLDEAGVDIVLVGDSLGMVVLGFPDTTH
CCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHCCCEEEEECCCCCEEEEECCCCCH
VTLDHMLHHVEATARGVKNAMLVGDMPIHSYDTVEQAVQSAKKLFLAGADAVKLEGGAAQ
HHHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
AGNIRAIVEAGIPVVGHIGLLPQKVLEEGGYKIKGKSSAEEEALVRDVEAVAEAGACAVV
CCCEEEEEECCCCCEEHHCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEE
VEGVTPHAARQVTVHSSIPTLGIGSGSHTCDGDVVVIHDLVGAFPWFIPAFVKPRADVAG
EECCCCCHHEEEEEECCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHH
SMTAAVKEWMEDCYTEPGSISS
HHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNQSFEKAERIRACKNRRHVTLVTAYDYPTGRLLDEAGVDIVLVGDSLGMVVLGFPDTTH
CCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHCCCEEEEECCCCCEEEEECCCCCH
VTLDHMLHHVEATARGVKNAMLVGDMPIHSYDTVEQAVQSAKKLFLAGADAVKLEGGAAQ
HHHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
AGNIRAIVEAGIPVVGHIGLLPQKVLEEGGYKIKGKSSAEEEALVRDVEAVAEAGACAVV
CCCEEEEEECCCCCEEHHCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEE
VEGVTPHAARQVTVHSSIPTLGIGSGSHTCDGDVVVIHDLVGAFPWFIPAFVKPRADVAG
EECCCCCHHEEEEEECCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHH
SMTAAVKEWMEDCYTEPGSISS
HHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA