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The map label for this gene is folK [H]

Identifier: 187734768

GI number: 187734768

Start: 313530

End: 314039

Strand: Reverse

Name: folK [H]

Synonym: Amuc_0256

Alternate gene names: 187734768

Gene position: 314039-313530 (Counterclockwise)

Preceding gene: 187734769

Following gene: 187734767

Centisome position: 11.79

GC content: 52.55

Gene sequence:

>510_bases
ATGAAGAGAGCAGGCATTGCACTGGGGTCCAATCTTGGGGATAAAAACAGCCTGATGGTTCAGGCCAGGGATCATTTGTT
GCAGATGAGCCTGTCTGATGACCCTTTTTTGCAATCCTCCCTGTATGCCACCAGTCCTCAGGGATGTCCGTACGGTGCGG
ATGATTATTTGAATGCCGTGGTGGAATTTACCTGGCCAGGTACGGTGGAGGAATTGCTTGTGCATTGCCAGGGTATAGAA
CGGGTTCTGGGGCGCATTCGTTCCGGGATTCGCTGTGAACCCCGCACGGCGGATGTGGATATTATCTATGTGGGGGACAT
GGTGGTCAATGATCCTCCTCGTCTGATTCTCCCCCATCCCAGAGCCCATTTGAGGAAATTTGTTTTAAAACCTCTTTCCG
ATATCAGGCCGGAGCTTATCCTGCCGCGCCAGCATAAAACGGTTGCCTGCCTGTTGGCGGAATTGGATACGGATGAGGCT
GATCCGCTCCTGGTATCGGAACGCTGGTAA

Upstream 100 bases:

>100_bases
TCTGTATACCATGGAAGACGTTCTGGGGTTGTCCCAATTATAACTTTTTCGGCATCGTGGCCGTTCTTCTTTTCCGGAAC
GGCCACGATGTTTTTTGCTG

Downstream 100 bases:

>100_bases
ATTGTATGTTTATTGAACGCCATGAATCAATCTTTTGAGAAAGCGGAACGAATCCGCGCCTGCAAGAACAGGCGGCATGT
GACTCTGGTTACTGCTTATG

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MKRAGIALGSNLGDKNSLMVQARDHLLQMSLSDDPFLQSSLYATSPQGCPYGADDYLNAVVEFTWPGTVEELLVHCQGIE
RVLGRIRSGIRCEPRTADVDIIYVGDMVVNDPPRLILPHPRAHLRKFVLKPLSDIRPELILPRQHKTVACLLAELDTDEA
DPLLVSERW

Sequences:

>Translated_169_residues
MKRAGIALGSNLGDKNSLMVQARDHLLQMSLSDDPFLQSSLYATSPQGCPYGADDYLNAVVEFTWPGTVEELLVHCQGIE
RVLGRIRSGIRCEPRTADVDIIYVGDMVVNDPPRLILPHPRAHLRKFVLKPLSDIRPELILPRQHKTVACLLAELDTDEA
DPLLVSERW
>Mature_169_residues
MKRAGIALGSNLGDKNSLMVQARDHLLQMSLSDDPFLQSSLYATSPQGCPYGADDYLNAVVEFTWPGTVEELLVHCQGIE
RVLGRIRSGIRCEPRTADVDIIYVGDMVVNDPPRLILPHPRAHLRKFVLKPLSDIRPELILPRQHKTVACLLAELDTDEA
DPLLVSERW

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=146, Percent_Identity=42.4657534246575, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 18893; Mature: 18893

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRAGIALGSNLGDKNSLMVQARDHLLQMSLSDDPFLQSSLYATSPQGCPYGADDYLNAV
CCCCCEEECCCCCCCCCEEEEEHHHHHEECCCCCCCHHHCCCCCCCCCCCCCHHHHHHHH
VEFTWPGTVEELLVHCQGIERVLGRIRSGIRCEPRTADVDIIYVGDMVVNDPPRLILPHP
EEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEECCCCCEEECCC
RAHLRKFVLKPLSDIRPELILPRQHKTVACLLAELDTDEADPLLVSERW
HHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHCCCCCCCCEEEECCC
>Mature Secondary Structure
MKRAGIALGSNLGDKNSLMVQARDHLLQMSLSDDPFLQSSLYATSPQGCPYGADDYLNAV
CCCCCEEECCCCCCCCCEEEEEHHHHHEECCCCCCCHHHCCCCCCCCCCCCCHHHHHHHH
VEFTWPGTVEELLVHCQGIERVLGRIRSGIRCEPRTADVDIIYVGDMVVNDPPRLILPHP
EEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEECCCCCEEECCC
RAHLRKFVLKPLSDIRPELILPRQHKTVACLLAELDTDEADPLLVSERW
HHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]