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The map label for this gene is kdsB [H]

Identifier: 187734682

GI number: 187734682

Start: 207638

End: 208387

Strand: Direct

Name: kdsB [H]

Synonym: Amuc_0169

Alternate gene names: 187734682

Gene position: 207638-208387 (Clockwise)

Preceding gene: 187734681

Following gene: 187734683

Centisome position: 7.79

GC content: 60.53

Gene sequence:

>750_bases
ATGGCAGCAGATACTTCCCATCATATCATCGGCCTCATTCCGTCACGCTGGGGTTCCTCCAGATTTCCGGGCAAGCCCCT
TCATCCCATTGCAGGAAAACCTCTTGTCCAGCACGTATGGGAACGCGTCTGCCGCTGTTCCCGCCTGGACGCCATCGCCA
TCGCCACGGACGACCAGCGCATTTTTGACGCCGCCGTATCCTTTGGAGCCAGGGCCATCATGACCTCTCCGGACCATCCC
AGCGGCAGTGACCGCCTGGCGGAAGCGGTACGTTCCTTCCCTGCCGCCACCCATGTCGTCAATATTCAGGGGGACGAACC
TCTCATTGACCCGGCCCTGATTGACAGGCTGGCGGAAGCCCTGGTCTCGGACGAAGCCCTCTCCATGGCCACCGTCGCCT
GCCCCATCAGCACCCGGGAGGATCTGGACAACCCGAACATTGTCAAAGTGGCCCTTGCCCGTAACGGAGACGCCCTGTAC
TTCTCCCGTTCCGTCATTCCCTATGCCCGCCACCCCCGCGTAGCACCGCCTTTGCGCCATCTGGGTATTTACGCCTACCG
CCGCGACTTCCTTGAAAACTATGTGCGCTGGGCCCCGACCCCCCTGGAACAGACGGAATCCCTGGAACAACTGCGCGCAC
TGGAAAACGGGGCCAGAATCCGGGTCATCCTGACGGACCACGTCAGTGTGGGCGTGGATACGCCGGAACAGGCGGAACAG
GTGGAACAAATCTTATTAAACATACACTAG

Upstream 100 bases:

>100_bases
CCGGCTGACTGTTCCTTCCCGGGAAAAACAGGCGTCCCCTATGCTTGAGGCTGGACTAATTCAAAGGGAGTTGCTACCTT
TCGGGGCGGTTCCTTTTCCC

Downstream 100 bases:

>100_bases
GAAGCAAATCATGAAATATATCTTCGTCACAGGCGGTGTTGTCTCCTCTCTCGGCAAAGGACTGGCGGCGGCATCCATCG
GCACGCTGCTGGAACGCTGC

Product: 3-deoxy-D-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MAADTSHHIIGLIPSRWGSSRFPGKPLHPIAGKPLVQHVWERVCRCSRLDAIAIATDDQRIFDAAVSFGARAIMTSPDHP
SGSDRLAEAVRSFPAATHVVNIQGDEPLIDPALIDRLAEALVSDEALSMATVACPISTREDLDNPNIVKVALARNGDALY
FSRSVIPYARHPRVAPPLRHLGIYAYRRDFLENYVRWAPTPLEQTESLEQLRALENGARIRVILTDHVSVGVDTPEQAEQ
VEQILLNIH

Sequences:

>Translated_249_residues
MAADTSHHIIGLIPSRWGSSRFPGKPLHPIAGKPLVQHVWERVCRCSRLDAIAIATDDQRIFDAAVSFGARAIMTSPDHP
SGSDRLAEAVRSFPAATHVVNIQGDEPLIDPALIDRLAEALVSDEALSMATVACPISTREDLDNPNIVKVALARNGDALY
FSRSVIPYARHPRVAPPLRHLGIYAYRRDFLENYVRWAPTPLEQTESLEQLRALENGARIRVILTDHVSVGVDTPEQAEQ
VEQILLNIH
>Mature_248_residues
AADTSHHIIGLIPSRWGSSRFPGKPLHPIAGKPLVQHVWERVCRCSRLDAIAIATDDQRIFDAAVSFGARAIMTSPDHPS
GSDRLAEAVRSFPAATHVVNIQGDEPLIDPALIDRLAEALVSDEALSMATVACPISTREDLDNPNIVKVALARNGDALYF
SRSVIPYARHPRVAPPLRHLGIYAYRRDFLENYVRWAPTPLEQTESLEQLRALENGARIRVILTDHVSVGVDTPEQAEQV
EQILLNIH

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria [H]

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family [H]

Homologues:

Organism=Escherichia coli, GI1787147, Length=241, Percent_Identity=46.4730290456432, Blast_Score=188, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003329
- InterPro:   IPR004528 [H]

Pfam domain/function: PF02348 CTP_transf_3 [H]

EC number: =2.7.7.38 [H]

Molecular weight: Translated: 27492; Mature: 27360

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADTSHHIIGLIPSRWGSSRFPGKPLHPIAGKPLVQHVWERVCRCSRLDAIAIATDDQR
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHH
IFDAAVSFGARAIMTSPDHPSGSDRLAEAVRSFPAATHVVNIQGDEPLIDPALIDRLAEA
HHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHH
LVSDEALSMATVACPISTREDLDNPNIVKVALARNGDALYFSRSVIPYARHPRVAPPLRH
HHHHHHHHHHHEECCCCCHHCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCHHH
LGIYAYRRDFLENYVRWAPTPLEQTESLEQLRALENGARIRVILTDHVSVGVDTPEQAEQ
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCHHHHHH
VEQILLNIH
HHHHHHCCC
>Mature Secondary Structure 
AADTSHHIIGLIPSRWGSSRFPGKPLHPIAGKPLVQHVWERVCRCSRLDAIAIATDDQR
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHH
IFDAAVSFGARAIMTSPDHPSGSDRLAEAVRSFPAATHVVNIQGDEPLIDPALIDRLAEA
HHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHH
LVSDEALSMATVACPISTREDLDNPNIVKVALARNGDALYFSRSVIPYARHPRVAPPLRH
HHHHHHHHHHHEECCCCCHHCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCHHH
LGIYAYRRDFLENYVRWAPTPLEQTESLEQLRALENGARIRVILTDHVSVGVDTPEQAEQ
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCHHHHHH
VEQILLNIH
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11889109 [H]