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The map label for this gene is hemK [H]

Identifier: 187734558

GI number: 187734558

Start: 58390

End: 59295

Strand: Direct

Name: hemK [H]

Synonym: Amuc_0042

Alternate gene names: 187734558

Gene position: 58390-59295 (Clockwise)

Preceding gene: 187734557

Following gene: 187734559

Centisome position: 2.19

GC content: 57.73

Gene sequence:

>906_bases
ATGAAAACTTTACTGGAAGTCCTTCAGGCCGGCACGGATTATCTGGCCCGCCAGGGGTGCGACGAGGCACGCGCCACCAT
GCAGCATTTGCTGGCCCATGTCCTGCACTGCAACCGCACGGCTCTTTACTCCCAGTTTGACAGGCCCGTGGAAGAAGCGG
AGCTGGCACCCCTGCGCGAACTGCTGAAACGCAGGGCAGCGGGAGAACCGCTGCAGCATCTGCTGGGGGTCACGGAATTT
TTCCGGAGAGATTTCCTGACGGATGCCCGCGCCCTGATTCCCCGCCCGGAAACCGAGGAACTGGTGGAAATGGTGTTAAA
GAAAATCCCGGACCATCCGGTACGCATCCTGGACATGGGCACGGGCTCCGGCGTTATCGGCGTCACGCTGGCGCTGGAAC
TCAAGGAGCGCGCCGGAGAAGTCGTGCTGGCGGACATTTCCCCGCAGGCTCTGGATCTGGCCCTGGAAAATGCCATGCGG
CTGGGAGCCAGAGTCTCCACCATCCAGACGAATCTGTTCGCCAACATTTCCCAGGAAAAAACGGATCCGCATGCGGAGGA
TGCCGATTCTGCTCCGGAGGGAAAAAAAGGGGAAAACGGGCGGAACATGCTCTTTGACGTTATCGTGGCCAATCTGCCAT
ACATTGCAGAGGGGGAAAAACTTGCCCCGGAAGTCATGAAAGACCCCCATACAGCCCTGTTCGGAGGCCCTAAAGGCTGG
GAAATCATTGAACGCTTTCTGGCCCGCGCCAGGGATTACCTGAACGAAGACGGCTTTGTAGCCCTGGAAATCGGGTACGA
CCAGGCATCCGTCGTAACGCAAATCATGGATGGATACGGCTACAATTACATTGAAGTTCTGAAGGACATGAGCGGCGTCT
CCCGTTTCCCCTTCGGCTATCGTTAA

Upstream 100 bases:

>100_bases
CTGATGGGGCAGCTGGAAGACCTGATTATGGCCCTCCAGCATGCGGAAATGCAGGAACGCCTGGCGGAGGCCGGAATGTC
CTAACTTCCTTTTTCCTTTC

Downstream 100 bases:

>100_bases
TGTTATCCTCCTTTTCACTCCATGCAGGCGGCGCGCATTCTGGTTGACGGACAAAGCGATCTGGTACTGGATTACGGTAT
TCCTCCGGAAGCGGGAGACG

Product: protein-(glutamine-N5) methyltransferase, release factor-specific

Products: NA

Alternate protein names: HemK protein homolog; M.RcoHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKTLLEVLQAGTDYLARQGCDEARATMQHLLAHVLHCNRTALYSQFDRPVEEAELAPLRELLKRRAAGEPLQHLLGVTEF
FRRDFLTDARALIPRPETEELVEMVLKKIPDHPVRILDMGTGSGVIGVTLALELKERAGEVVLADISPQALDLALENAMR
LGARVSTIQTNLFANISQEKTDPHAEDADSAPEGKKGENGRNMLFDVIVANLPYIAEGEKLAPEVMKDPHTALFGGPKGW
EIIERFLARARDYLNEDGFVALEIGYDQASVVTQIMDGYGYNYIEVLKDMSGVSRFPFGYR

Sequences:

>Translated_301_residues
MKTLLEVLQAGTDYLARQGCDEARATMQHLLAHVLHCNRTALYSQFDRPVEEAELAPLRELLKRRAAGEPLQHLLGVTEF
FRRDFLTDARALIPRPETEELVEMVLKKIPDHPVRILDMGTGSGVIGVTLALELKERAGEVVLADISPQALDLALENAMR
LGARVSTIQTNLFANISQEKTDPHAEDADSAPEGKKGENGRNMLFDVIVANLPYIAEGEKLAPEVMKDPHTALFGGPKGW
EIIERFLARARDYLNEDGFVALEIGYDQASVVTQIMDGYGYNYIEVLKDMSGVSRFPFGYR
>Mature_301_residues
MKTLLEVLQAGTDYLARQGCDEARATMQHLLAHVLHCNRTALYSQFDRPVEEAELAPLRELLKRRAAGEPLQHLLGVTEF
FRRDFLTDARALIPRPETEELVEMVLKKIPDHPVRILDMGTGSGVIGVTLALELKERAGEVVLADISPQALDLALENAMR
LGARVSTIQTNLFANISQEKTDPHAEDADSAPEGKKGENGRNMLFDVIVANLPYIAEGEKLAPEVMKDPHTALFGGPKGW
EIIERFLARARDYLNEDGFVALEIGYDQASVVTQIMDGYGYNYIEVLKDMSGVSRFPFGYR

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=320, Percent_Identity=31.5625, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1787463, Length=285, Percent_Identity=31.2280701754386, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI87082085, Length=225, Percent_Identity=28, Blast_Score=78, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24582226, Length=288, Percent_Identity=29.1666666666667, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 33493; Mature: 33493

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLLEVLQAGTDYLARQGCDEARATMQHLLAHVLHCNRTALYSQFDRPVEEAELAPLRE
CHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHH
LLKRRAAGEPLQHLLGVTEFFRRDFLTDARALIPRPETEELVEMVLKKIPDHPVRILDMG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECC
TGSGVIGVTLALELKERAGEVVLADISPQALDLALENAMRLGARVSTIQTNLFANISQEK
CCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHC
TDPHAEDADSAPEGKKGENGRNMLFDVIVANLPYIAEGEKLAPEVMKDPHTALFGGPKGW
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCHHHHCCCCCCCCCCCCCH
EIIERFLARARDYLNEDGFVALEIGYDQASVVTQIMDGYGYNYIEVLKDMSGVSRFPFGY
HHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
R
C
>Mature Secondary Structure
MKTLLEVLQAGTDYLARQGCDEARATMQHLLAHVLHCNRTALYSQFDRPVEEAELAPLRE
CHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHH
LLKRRAAGEPLQHLLGVTEFFRRDFLTDARALIPRPETEELVEMVLKKIPDHPVRILDMG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECC
TGSGVIGVTLALELKERAGEVVLADISPQALDLALENAMRLGARVSTIQTNLFANISQEK
CCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHC
TDPHAEDADSAPEGKKGENGRNMLFDVIVANLPYIAEGEKLAPEVMKDPHTALFGGPKGW
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCHHHHCCCCCCCCCCCCCH
EIIERFLARARDYLNEDGFVALEIGYDQASVVTQIMDGYGYNYIEVLKDMSGVSRFPFGY
HHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]