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The map label for this gene is prfA

Identifier: 187734557

GI number: 187734557

Start: 57297

End: 58373

Strand: Direct

Name: prfA

Synonym: Amuc_0041

Alternate gene names: 187734557

Gene position: 57297-58373 (Clockwise)

Preceding gene: 187734556

Following gene: 187734558

Centisome position: 2.15

GC content: 58.22

Gene sequence:

>1077_bases
ATGGATTACACACCTCTCATTGAAAAACGCCGCCAGCGCCTTGAAGAATTGGAAACAGTCATTGCCGAACCGGACTTCTT
CAATGACCAGAAAAAGGCCTCGGAAATCATGAGAGAGCACCGCCGCCTGAAGGAACTCATGGAAACATGGGATTCCCTGA
ACGCCACGGAACAACAGCTGGCGGACAACCAGGAACTGGCCAAGACGGACGATCCGGAATTAGCGGAACTGGCCGCCCTG
GAGATTCCAGAACTGGAAGCCGCCCTGGAAAAACTGCGGAGCGACGTGCAGTACAGCCTGCTACCCCGCGACACGACGGA
AGACCGGGACGCCATCATTGAAATCCGCGCCGGAACGGGCGGGGATGAAGCCTCCCTGTTTGCCGGAGACCTGCTGCGGA
TGTACCAGCGTTTTGCGGAAGAACGCGGCTGGCGCTTCGAGCATCTGGAAAGCAGCCCGTCGGACGTGGGCGGATTCAAG
GAAGTCGTCTGCCGCATTGCCGGGGAAGAAGTGTTCCGCTTCCTGAAATATGAAGGCGGCGTGCACCGTGTGCAGCGCGT
TCCCGCTACGGAAACGCAGGGGCGCATCCATACTTCCACCGCCACCGTGGCCGTCATGCCGGAAGCGGAGGAAGTGGACA
TAGAAATCCGCCCGGAAGATCTCCGCATCGAAGTATGCCGTTCCGGCGGAGCCGGAGGCCAGCATGTGAACAAAACGGAA
TCCGCCGTTCAGATATGGCACATACCCACGGGCGTTTATGTCCGGTGCGAGGAAGAGCGCAGCCAGATGAAAAACCGAGA
AAAAGGCATGAAAATCCTGCGCGCCAAGCTCTTTGAAGCCAAAAAACGGGAAGAGGCGGAAAAATACTCCGCCGCGCGCC
GCAATCTCATCGGCTCCGGCGGCCGTGAAGAAAAGATACGCACATACAACTTCCCGCAGAACCGTCTGACAGACCACCGC
ATCGGCTACACGTCCCACAATCTGGACGGTATCCTGATGGGGCAGCTGGAAGACCTGATTATGGCCCTCCAGCATGCGGA
AATGCAGGAACGCCTGGCGGAGGCCGGAATGTCCTAA

Upstream 100 bases:

>100_bases
ATCCCTCCCTGCTTTTAAATGCCGGAACATGTCTTACATCCCCCGGATTTGGCTTTCAGAAAGCCCCGCCATCTTTTAAA
ATCCTTTACACGCAAACGCC

Downstream 100 bases:

>100_bases
CTTCCTTTTTCCTTTCATGAAAACTTTACTGGAAGTCCTTCAGGCCGGCACGGATTATCTGGCCCGCCAGGGGTGCGACG
AGGCACGCGCCACCATGCAG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MDYTPLIEKRRQRLEELETVIAEPDFFNDQKKASEIMREHRRLKELMETWDSLNATEQQLADNQELAKTDDPELAELAAL
EIPELEAALEKLRSDVQYSLLPRDTTEDRDAIIEIRAGTGGDEASLFAGDLLRMYQRFAEERGWRFEHLESSPSDVGGFK
EVVCRIAGEEVFRFLKYEGGVHRVQRVPATETQGRIHTSTATVAVMPEAEEVDIEIRPEDLRIEVCRSGGAGGQHVNKTE
SAVQIWHIPTGVYVRCEEERSQMKNREKGMKILRAKLFEAKKREEAEKYSAARRNLIGSGGREEKIRTYNFPQNRLTDHR
IGYTSHNLDGILMGQLEDLIMALQHAEMQERLAEAGMS

Sequences:

>Translated_358_residues
MDYTPLIEKRRQRLEELETVIAEPDFFNDQKKASEIMREHRRLKELMETWDSLNATEQQLADNQELAKTDDPELAELAAL
EIPELEAALEKLRSDVQYSLLPRDTTEDRDAIIEIRAGTGGDEASLFAGDLLRMYQRFAEERGWRFEHLESSPSDVGGFK
EVVCRIAGEEVFRFLKYEGGVHRVQRVPATETQGRIHTSTATVAVMPEAEEVDIEIRPEDLRIEVCRSGGAGGQHVNKTE
SAVQIWHIPTGVYVRCEEERSQMKNREKGMKILRAKLFEAKKREEAEKYSAARRNLIGSGGREEKIRTYNFPQNRLTDHR
IGYTSHNLDGILMGQLEDLIMALQHAEMQERLAEAGMS
>Mature_358_residues
MDYTPLIEKRRQRLEELETVIAEPDFFNDQKKASEIMREHRRLKELMETWDSLNATEQQLADNQELAKTDDPELAELAAL
EIPELEAALEKLRSDVQYSLLPRDTTEDRDAIIEIRAGTGGDEASLFAGDLLRMYQRFAEERGWRFEHLESSPSDVGGFK
EVVCRIAGEEVFRFLKYEGGVHRVQRVPATETQGRIHTSTATVAVMPEAEEVDIEIRPEDLRIEVCRSGGAGGQHVNKTE
SAVQIWHIPTGVYVRCEEERSQMKNREKGMKILRAKLFEAKKREEAEKYSAARRNLIGSGGREEKIRTYNFPQNRLTDHR
IGYTSHNLDGILMGQLEDLIMALQHAEMQERLAEAGMS

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=343, Percent_Identity=45.4810495626822, Blast_Score=294, Evalue=8e-80,
Organism=Homo sapiens, GI34577120, Length=328, Percent_Identity=39.3292682926829, Blast_Score=251, Evalue=5e-67,
Organism=Homo sapiens, GI166795305, Length=237, Percent_Identity=43.4599156118143, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI1787462, Length=352, Percent_Identity=46.0227272727273, Blast_Score=301, Evalue=4e-83,
Organism=Escherichia coli, GI2367172, Length=346, Percent_Identity=37.2832369942197, Blast_Score=217, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI17542784, Length=320, Percent_Identity=35, Blast_Score=188, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=327, Percent_Identity=39.1437308868502, Blast_Score=220, Evalue=3e-58,
Organism=Drosophila melanogaster, GI19921226, Length=246, Percent_Identity=50, Blast_Score=240, Evalue=1e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_AKKM8 (B2UL99)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001876669.1
- ProteinModelPortal:   B2UL99
- SMR:   B2UL99
- GeneID:   6275158
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_0041
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40974; Mature: 40974

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYTPLIEKRRQRLEELETVIAEPDFFNDQKKASEIMREHRRLKELMETWDSLNATEQQL
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ADNQELAKTDDPELAELAALEIPELEAALEKLRSDVQYSLLPRDTTEDRDAIIEIRAGTG
HCCCHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCCC
GDEASLFAGDLLRMYQRFAEERGWRFEHLESSPSDVGGFKEVVCRIAGEEVFRFLKYEGG
CCHHHHHHHHHHHHHHHHHHHCCCEEHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VHRVQRVPATETQGRIHTSTATVAVMPEAEEVDIEIRPEDLRIEVCRSGGAGGQHVNKTE
HHHHHCCCCCCCCCEEEECCEEEEECCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCC
SAVQIWHIPTGVYVRCEEERSQMKNREKGMKILRAKLFEAKKREEAEKYSAARRNLIGSG
CCEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GREEKIRTYNFPQNRLTDHRIGYTSHNLDGILMGQLEDLIMALQHAEMQERLAEAGMS
CCCCCCEECCCCHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDYTPLIEKRRQRLEELETVIAEPDFFNDQKKASEIMREHRRLKELMETWDSLNATEQQL
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ADNQELAKTDDPELAELAALEIPELEAALEKLRSDVQYSLLPRDTTEDRDAIIEIRAGTG
HCCCHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCCC
GDEASLFAGDLLRMYQRFAEERGWRFEHLESSPSDVGGFKEVVCRIAGEEVFRFLKYEGG
CCHHHHHHHHHHHHHHHHHHHCCCEEHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VHRVQRVPATETQGRIHTSTATVAVMPEAEEVDIEIRPEDLRIEVCRSGGAGGQHVNKTE
HHHHHCCCCCCCCCEEEECCEEEEECCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCC
SAVQIWHIPTGVYVRCEEERSQMKNREKGMKILRAKLFEAKKREEAEKYSAARRNLIGSG
CCEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GREEKIRTYNFPQNRLTDHRIGYTSHNLDGILMGQLEDLIMALQHAEMQERLAEAGMS
CCCCCCEECCCCHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA