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The map label for this gene is outE [H]

Identifier: 186477815

GI number: 186477815

Start: 3451043

End: 3452590

Strand: Direct

Name: outE [H]

Synonym: Bphy_3071

Alternate gene names: 186477815

Gene position: 3451043-3452590 (Clockwise)

Preceding gene: 186477814

Following gene: 186477816

Centisome position: 99.19

GC content: 64.21

Gene sequence:

>1548_bases
GTGAGCTCGACGCCGCTTTCCGGGCCGCACGCCTCTGCCGGATCATCGGACGATGCGCGCGGCGCGCCGGAAACGCCCCC
GACCCAGACAGGACGCGAAGCGCCCTCTCCGCTTGCTGCGCGGCTCGTGCCGTACGGATTCGCGAAAAGCGGCCAGATCC
TGGTCGCGCATCAGCACGCCGATTCGATGGAAGTCTGGATGAGCGAACGCACGAACCAGGCGGCGCTTGCCGAAGTCGTG
CGCAATTTCGGCGCGATATCGGTCGTGCGCATGCCAGCGGACGAATTGGCGCAAGCCATCAACCAGGCGTACGCGCGTCA
GGACGGGAGCGCTGCGCAGGTGGTCGGCGAAGTGGAAGGCGAAGTCGATTTGTCGCGTCTGATGCAGGACATTCCCGAAG
TGGAAGACCTGCTGGAGTCCGAAGACGACGCGCCGATCATCCGCATGATCAACGCGCTGCTCACGCAGGCAGCGCGCGAA
CAGGCATCGGATATTCACATCGAGCCATTCGAAAACGCATCGGTGGTTCGCTTTCGCGTCGACGGCACGTTGCGCGACGT
CGTGCGTCCGAAGAAGGCGCTGCACGGCGCGTTGATTTCGCGGATCAAGATCATGGCGCAACTCGACATTGCCGAGAAGC
GTCTGCCGCAGGATGGCCGTATCACGCTGCGCGTCGGCGGAAGGCCCGTCGACGTGCGCGTGTCGACGTTGCCGACGGGC
CACGGCGAACGCGCCGTGCTGCGTCTGCTGGAAAAAGACGCGCAGCGTCTGAATCTCGAAGCGCTCGGCATGGCATCCGA
CACGCTCAAGCAGTTCGACAAGCTGATTTCGCGTCCGCACGGCATCGTGCTCGTGACGGGCCCGACGGGCTCGGGCAAGA
CGACCACGCTCTATGCGTCGATGTCGCGGCTCGAAACGGCGACGACCAACATCATGACCGTCGAAGACCCGATCGAATAC
GATCTGCCGGGCATTGGTCAGACTCAGGTCAACGAGCGGATCGGCATGACGTTCGCCCGCGCGCTGCGTTCCATTCTTCG
TCAGGATCCCGACATCATCATGATCGGTGAGATCCGCGATCTGGAAACGGCGCAGATTGCCGTGCAGGCATCGCTGACGG
GACACCTGGTACTCGCTACGCTGCACACCAATGACGCCGCCTCCGCCGTCACGCGTCTGACGGATATGGGCGTCGAGCCC
TATCTGCTCGCGTCGTCGCTGCTGGGCGTGCTGGCGCAGCGTCTCGTACGGCGGCTGTGTCCCGTGTGCAAGGAAGCGCG
CGAAGAAGAAGGCGGCCGCAAGGTGTGGCATCCCGTCGGTTGCGACAAGTGCGGCCATTCGGGTTATGCGGGCCGTCGCG
GCGTGTATGAACTGCTCAATGTCGACGAACCGATTCGCGCGCTGATCCACCGCAATGCTGCCGACGCCGAGATTGCCGAA
GCCGGCCGTCATCAAGGCATGCGCACCTTGCGCGAGGACGGCGAACGATGGCTCGCGTCGGGCATGACGTCGCTCGAAGA
AGTGATACGCGTGACGGGCGGGGTCTGA

Upstream 100 bases:

>100_bases
AGCCCCAAGTTCAGCCGCAGACGCAACCGCAGCCGCTGCAGGTCGAACCGGCGCAAACCGTGCCGGCTCAACCGGCCACG
AGTACGCAAAAGGTGCAGCC

Downstream 100 bases:

>100_bases
GGGCATGCCGGCATTCCGCTTCGAAGCGATCGACGCCGCAGGCAAGGCGCAAAAAGGCGTACTCGATGCTGACAGCGCGC
GCGGCGCGCGTACGCAGTTG

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Pectic enzymes secretion protein outE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSSTPLSGPHASAGSSDDARGAPETPPTQTGREAPSPLAARLVPYGFAKSGQILVAHQHADSMEVWMSERTNQAALAEVV
RNFGAISVVRMPADELAQAINQAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAARE
QASDIHIEPFENASVVRFRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTG
HGERAVLRLLEKDAQRLNLEALGMASDTLKQFDKLISRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEY
DLPGIGQTQVNERIGMTFARALRSILRQDPDIIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEP
YLLASSLLGVLAQRLVRRLCPVCKEAREEEGGRKVWHPVGCDKCGHSGYAGRRGVYELLNVDEPIRALIHRNAADAEIAE
AGRHQGMRTLREDGERWLASGMTSLEEVIRVTGGV

Sequences:

>Translated_515_residues
MSSTPLSGPHASAGSSDDARGAPETPPTQTGREAPSPLAARLVPYGFAKSGQILVAHQHADSMEVWMSERTNQAALAEVV
RNFGAISVVRMPADELAQAINQAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAARE
QASDIHIEPFENASVVRFRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTG
HGERAVLRLLEKDAQRLNLEALGMASDTLKQFDKLISRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEY
DLPGIGQTQVNERIGMTFARALRSILRQDPDIIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEP
YLLASSLLGVLAQRLVRRLCPVCKEAREEEGGRKVWHPVGCDKCGHSGYAGRRGVYELLNVDEPIRALIHRNAADAEIAE
AGRHQGMRTLREDGERWLASGMTSLEEVIRVTGGV
>Mature_514_residues
SSTPLSGPHASAGSSDDARGAPETPPTQTGREAPSPLAARLVPYGFAKSGQILVAHQHADSMEVWMSERTNQAALAEVVR
NFGAISVVRMPADELAQAINQAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQ
ASDIHIEPFENASVVRFRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGH
GERAVLRLLEKDAQRLNLEALGMASDTLKQFDKLISRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEYD
LPGIGQTQVNERIGMTFARALRSILRQDPDIIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPY
LLASSLLGVLAQRLVRRLCPVCKEAREEEGGRKVWHPVGCDKCGHSGYAGRRGVYELLNVDEPIRALIHRNAADAEIAEA
GRHQGMRTLREDGERWLASGMTSLEEVIRVTGGV

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pectate lyases and cellulases across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=420, Percent_Identity=54.5238095238095, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1786296, Length=439, Percent_Identity=43.0523917995444, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI87082188, Length=195, Percent_Identity=44.6153846153846, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 55848; Mature: 55717

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTPLSGPHASAGSSDDARGAPETPPTQTGREAPSPLAARLVPYGFAKSGQILVAHQHA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCC
DSMEVWMSERTNQAALAEVVRNFGAISVVRMPADELAQAINQAYARQDGSAAQVVGEVEG
CHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHCCC
EVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRFRV
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
DGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCC
HGERAVLRLLEKDAQRLNLEALGMASDTLKQFDKLISRPHGIVLVTGPTGSGKTTTLYAS
CCHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEHHH
MSRLETATTNIMTVEDPIEYDLPGIGQTQVNERIGMTFARALRSILRQDPDIIMIGEIRD
HHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
LETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRRLC
CCHHHEEEEEECCCEEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
PVCKEAREEEGGRKVWHPVGCDKCGHSGYAGRRGVYELLNVDEPIRALIHRNAADAEIAE
HHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCHHHHH
AGRHQGMRTLREDGERWLASGMTSLEEVIRVTGGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSTPLSGPHASAGSSDDARGAPETPPTQTGREAPSPLAARLVPYGFAKSGQILVAHQHA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCC
DSMEVWMSERTNQAALAEVVRNFGAISVVRMPADELAQAINQAYARQDGSAAQVVGEVEG
CHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHCCC
EVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRFRV
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
DGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCC
HGERAVLRLLEKDAQRLNLEALGMASDTLKQFDKLISRPHGIVLVTGPTGSGKTTTLYAS
CCHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEHHH
MSRLETATTNIMTVEDPIEYDLPGIGQTQVNERIGMTFARALRSILRQDPDIIMIGEIRD
HHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
LETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRRLC
CCHHHEEEEEECCCEEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
PVCKEAREEEGGRKVWHPVGCDKCGHSGYAGRRGVYELLNVDEPIRALIHRNAADAEIAE
HHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCHHHHH
AGRHQGMRTLREDGERWLASGMTSLEEVIRVTGGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]