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The map label for this gene is hemA

Identifier: 186477468

GI number: 186477468

Start: 3075470

End: 3076759

Strand: Direct

Name: hemA

Synonym: Bphy_2720

Alternate gene names: 186477468

Gene position: 3075470-3076759 (Clockwise)

Preceding gene: 186477467

Following gene: 186477469

Centisome position: 88.4

GC content: 64.19

Gene sequence:

>1290_bases
GTGCAACTGCTAACGATCGGAATCAATCACCACACAGCGCCTGTCGCCCTGCGCGAACGCGTGGCATTTCCGCTCGAACA
GATCAAGCCGGCGCTTTCTACGTTCAAGGACATCTTTCTGGGTCGCACGGCTCGCACCGCGCCGGAAGCAGCCATCCTGT
CGACCTGCAATCGCACCGAGCTCTACTGCGCCACCGACGACCACGCGGCCCGCGAAGCCGCGATTCACTGGCTGTCGGAG
TACCACAACATCGCCATCGACGAACTCGCGCCGCACGTTTATGCGCTGCCGCAGTCGGAAGCGGTGCGCCATGCGTTTCG
CGTCGCGTCGGGGCTGGATTCGATGGTGCTGGGGGAAACGCAGATCGTCGGTCAGATGAAGGATGCCGTGCGCACGGCGA
CGGAAGCCGGGGCGCTCGGTACCTATCTGAACCAGTTGTTCCAGCGCACCTTCGCGGTCGCGAAGGAAGTCCGCACCACG
ACTGAAATCGGGGCGCAATCCGTTTCCATGGCTGCGGCGGCTGTACGCCTCGCGCAGCGCATTTTCGACAAGGTGTCGAA
CCAGCGCGTGCTGTTCATCGGCGCGGGCGAAATGATCGAATTGTGCGCGACTCACTTCGCCGCACAGCAGCCGCGCGAGC
TAGTCGTCGCAAACCGCACGGCCGAACGCGGCCAGCGCCTTGCCGAGAGCTACAACGGCCGCGCGATTCCGCTGTCCGAG
TTGCCGACGCGCATGCACGAGTTCGACATCATCGTATCCTGCACGGCGTCGACGCTGCCCATCATCGGTCTCGGCGCGGT
TGAGCGCGCGGTGAAGGCGCGGCGTCATCGCCCCATTTTCATGGTCGATCTCGCCGTGCCGCGCGACATCGAACCGGAGG
TCGGCAAGCTCGAAGACGTGTTTCTGTACACCGTCGACGATCTCGGCGCGATCGTCCGCGAGGGCAATGCGTCGCGGCAA
GCTGCCGTCGCGCAGGCCGAAACCATCATCGAAACGCGCGTGCAGAATTTCATGCAGTGGCTGGACGCGCGCAGCATTGT
GCCCGTCATCCGTCACATGCACACCCAGGCCGACGCGCTGCGCCGCGCCGAAGTCGACAAGGCGCAGAAGATGCTCGCGC
GCGGCGACGATCCCGCCGCCGTGCTCGAAGCGCTGTCGCAGGCGCTGACCAACAAGCTGATCCACGGTCCGACGCATGCG
CTCAACCGTGCCAGCAGCGAAAACCGTGATCAGCTCATCGAATTGATGGGCGGCTTCTACAAGCACGCACACCCGTCTTC
CGAGCGTTAG

Upstream 100 bases:

>100_bases
ACGGATAAACCCCCGCCGGCTGAAAGAATCGTGGAAACGCTATAATATTGGGCTTAACCATAGCCCCAATACGTCGCGCC
AAAAACGCCGTAGCGTCCCC

Downstream 100 bases:

>100_bases
CGGCGCTTCTCGCCGCGGCAGCGCGCGGGTGGCCGACAGGCTCGCACGGTGCCTCATCTGCAGTGACAAGGGTGCTTTCG
CCCGCTTCCCCGAATCCAGC

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MQLLTIGINHHTAPVALRERVAFPLEQIKPALSTFKDIFLGRTARTAPEAAILSTCNRTELYCATDDHAAREAAIHWLSE
YHNIAIDELAPHVYALPQSEAVRHAFRVASGLDSMVLGETQIVGQMKDAVRTATEAGALGTYLNQLFQRTFAVAKEVRTT
TEIGAQSVSMAAAAVRLAQRIFDKVSNQRVLFIGAGEMIELCATHFAAQQPRELVVANRTAERGQRLAESYNGRAIPLSE
LPTRMHEFDIIVSCTASTLPIIGLGAVERAVKARRHRPIFMVDLAVPRDIEPEVGKLEDVFLYTVDDLGAIVREGNASRQ
AAVAQAETIIETRVQNFMQWLDARSIVPVIRHMHTQADALRRAEVDKAQKMLARGDDPAAVLEALSQALTNKLIHGPTHA
LNRASSENRDQLIELMGGFYKHAHPSSER

Sequences:

>Translated_429_residues
MQLLTIGINHHTAPVALRERVAFPLEQIKPALSTFKDIFLGRTARTAPEAAILSTCNRTELYCATDDHAAREAAIHWLSE
YHNIAIDELAPHVYALPQSEAVRHAFRVASGLDSMVLGETQIVGQMKDAVRTATEAGALGTYLNQLFQRTFAVAKEVRTT
TEIGAQSVSMAAAAVRLAQRIFDKVSNQRVLFIGAGEMIELCATHFAAQQPRELVVANRTAERGQRLAESYNGRAIPLSE
LPTRMHEFDIIVSCTASTLPIIGLGAVERAVKARRHRPIFMVDLAVPRDIEPEVGKLEDVFLYTVDDLGAIVREGNASRQ
AAVAQAETIIETRVQNFMQWLDARSIVPVIRHMHTQADALRRAEVDKAQKMLARGDDPAAVLEALSQALTNKLIHGPTHA
LNRASSENRDQLIELMGGFYKHAHPSSER
>Mature_429_residues
MQLLTIGINHHTAPVALRERVAFPLEQIKPALSTFKDIFLGRTARTAPEAAILSTCNRTELYCATDDHAAREAAIHWLSE
YHNIAIDELAPHVYALPQSEAVRHAFRVASGLDSMVLGETQIVGQMKDAVRTATEAGALGTYLNQLFQRTFAVAKEVRTT
TEIGAQSVSMAAAAVRLAQRIFDKVSNQRVLFIGAGEMIELCATHFAAQQPRELVVANRTAERGQRLAESYNGRAIPLSE
LPTRMHEFDIIVSCTASTLPIIGLGAVERAVKARRHRPIFMVDLAVPRDIEPEVGKLEDVFLYTVDDLGAIVREGNASRQ
AAVAQAETIIETRVQNFMQWLDARSIVPVIRHMHTQADALRRAEVDKAQKMLARGDDPAAVLEALSQALTNKLIHGPTHA
LNRASSENRDQLIELMGGFYKHAHPSSER

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=417, Percent_Identity=45.3237410071942, Blast_Score=350, Evalue=1e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_BURP8 (B2JHK7)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001858938.1
- ProteinModelPortal:   B2JHK7
- GeneID:   6244203
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2720
- HOGENOM:   HBG732626
- OMA:   GPILNRL
- ProtClustDB:   PRK00045
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 47266; Mature: 47266

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 56-56 BINDING 114-114 BINDING 125-125

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLLTIGINHHTAPVALRERVAFPLEQIKPALSTFKDIFLGRTARTAPEAAILSTCNRTE
CEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCE
LYCATDDHAAREAAIHWLSEYHNIAIDELAPHVYALPQSEAVRHAFRVASGLDSMVLGET
EEEECCCHHHHHHHHHHHHHHHCCHHHHHCCHHEECCCHHHHHHHHHHHHCCHHHHHCHH
QIVGQMKDAVRTATEAGALGTYLNQLFQRTFAVAKEVRTTTEIGAQSVSMAAAAVRLAQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFDKVSNQRVLFIGAGEMIELCATHFAAQQPRELVVANRTAERGQRLAESYNGRAIPLSE
HHHHHCCCEEEEEECCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCCCCCCHHH
LPTRMHEFDIIVSCTASTLPIIGLGAVERAVKARRHRPIFMVDLAVPRDIEPEVGKLEDV
HHHHHHHHHEEEEECCCCCCEEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCE
FLYTVDDLGAIVREGNASRQAAVAQAETIIETRVQNFMQWLDARSIVPVIRHMHTQADAL
EEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRAEVDKAQKMLARGDDPAAVLEALSQALTNKLIHGPTHALNRASSENRDQLIELMGGFY
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHH
KHAHPSSER
HCCCCCCCC
>Mature Secondary Structure
MQLLTIGINHHTAPVALRERVAFPLEQIKPALSTFKDIFLGRTARTAPEAAILSTCNRTE
CEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCE
LYCATDDHAAREAAIHWLSEYHNIAIDELAPHVYALPQSEAVRHAFRVASGLDSMVLGET
EEEECCCHHHHHHHHHHHHHHHCCHHHHHCCHHEECCCHHHHHHHHHHHHCCHHHHHCHH
QIVGQMKDAVRTATEAGALGTYLNQLFQRTFAVAKEVRTTTEIGAQSVSMAAAAVRLAQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFDKVSNQRVLFIGAGEMIELCATHFAAQQPRELVVANRTAERGQRLAESYNGRAIPLSE
HHHHHCCCEEEEEECCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCCCCCCHHH
LPTRMHEFDIIVSCTASTLPIIGLGAVERAVKARRHRPIFMVDLAVPRDIEPEVGKLEDV
HHHHHHHHHEEEEECCCCCCEEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCE
FLYTVDDLGAIVREGNASRQAAVAQAETIIETRVQNFMQWLDARSIVPVIRHMHTQADAL
EEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRAEVDKAQKMLARGDDPAAVLEALSQALTNKLIHGPTHALNRASSENRDQLIELMGGFY
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHH
KHAHPSSER
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA