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The map label for this gene is murD

Identifier: 186477423

GI number: 186477423

Start: 3017493

End: 3019007

Strand: Reverse

Name: murD

Synonym: Bphy_2675

Alternate gene names: 186477423

Gene position: 3019007-3017493 (Counterclockwise)

Preceding gene: 186477424

Following gene: 186477422

Centisome position: 86.77

GC content: 67.13

Gene sequence:

>1515_bases
ATGTTTGGCGAGAAGTTTCGGGATCGGCAAAAGCCGATGGTGCTCGTGCTGGGGCTCGGTGAATCGGGCCTTGCGATGGC
GCGCTGGTGCGCGCGGCATGGTTGCCGCCTGCGCATCGCCGACACGCGCGAAGTGCCGCCGAACCTGTCGGCGCTCGAAG
CGCACGGCATCGAAGGCAATTTTGTCGGCGGGCCGTTTTCCGAGGTGCTGCTGGAAGGGGTCGAACTGGTCGCGATCAGT
CCGGGCCTGTCGCCGCTCGCCGCCGACCTCGTGCCGCTGATCGCCACTGCCCGCGAGCGCGGCATTCCCGTGTGGGGCGA
ACTCGAGTTCTTCGCGCAGGCGCTGAAGACGCTCGGCGAAAGCGGCTACGCGCCGAAGGTGATCGCGATCACGGGCACGA
ACGGCAAGACGACCACGACGAGCCTCACCGGCTTGCTGTGCGAACGCGCGGGCAAGAAGGTCGCGGTCGCGGGCAACATC
AGCCCAGCCGCGCTCGACAAGCTCACGGAAGCGATCGACAACACCGCGCTGCCCGACGTATGGGTGCTCGAACTGTCGAG
CTTCCAGCTGGAGACAGCGCATACGTTCGAGCCCGACGCTGCCGTGATCCTCAATATCACGCAGGATCATCTGGATTGGC
ACGGCGGCCTCGACGCGTACGCCGCCGCGAAGGGCAAGATTTTCGGCAAGAACACCGTGCGCGTGCTCAACCGCGACGAC
GCGCGCGTGATGACACTCGCTTCCTCGGAGCAGAGCGGCGCGCAACTCGTGACGTTCGGCGTCAACGAACCGGTGCGCGA
TGGCGATTACGGGCTGCTGCGTGACAACGGCATGATCTGGCTTGTGCAGGCGCAAGACCGCGACGCCACCGACGAGCCCG
CGCCCACGCGCCGCCGCAAGAGCGAATCCACGGCGCCGCCGGATATCGGCCTGAAGCGCCTGATGCCCGCCGACGCGCTG
CGCATTCGCGGTCTGCACAACGCGACGAACGCGCTCGCCGCGTTCGCCTTGGCGCGCGCCATCGGTTTGCCCGGTGCGCC
GCTGTTGCATGGCTTGCGCGAGTATCGTGGGGAGCCGCATCGCGTGGAACTGATTGCGTCGATCGAAGGCGTCGATTACG
TCGACGACAGCAAGGGCACCAATGTCGGCGCGACGGTTGCGGCGCTCGACGGTCTCGCGCAACGTGTCGTGCTGATCGCG
GGCGGAGACGGCAAGGGTCAGGCATTCGATCCGCTCGCCGAACCCGTCACGCGCTGGTGCCGCTGCGTCATGCTGATCGG
CCGTGACGCGCCGCAGATTCGCGCCGCGCTCGAGCACACCGGCATCGCGATAACCGATCACGCCACGCTCGAAGAAGCGA
CGCGCGCCGCGAGCGCCGTCGCGCAACCGGGCGACGCCGTGCTGCTGTCGCCTGCATGCGCCAGCTTCGACATGTTCAAG
GGTTACGCACATCGCGCCGCGGTTTTCCGCAGCACGGTCGAAGACATCGCTGCCGGACGGGGGACGATGATATGA

Upstream 100 bases:

>100_bases
GTTGTGCCTGTTCGGTTTGTCCACGCTCAAGCTGCGTTAAGCCGCGGTTCGAGTCGCAATCAAAGTCGGATTCACAGTCG
CAAGCAAGGGGAAGCAGTCG

Downstream 100 bases:

>100_bases
GCTGGACGGAACGCTTCGGTTCGCAACTCGGCAAGCAACGCGGCTCGATCGCTGGGCAAACCGGCAGCGCGCCGGCTTCC
GAGCGCGCATCGCGCACGGG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MFGEKFRDRQKPMVLVLGLGESGLAMARWCARHGCRLRIADTREVPPNLSALEAHGIEGNFVGGPFSEVLLEGVELVAIS
PGLSPLAADLVPLIATARERGIPVWGELEFFAQALKTLGESGYAPKVIAITGTNGKTTTTSLTGLLCERAGKKVAVAGNI
SPAALDKLTEAIDNTALPDVWVLELSSFQLETAHTFEPDAAVILNITQDHLDWHGGLDAYAAAKGKIFGKNTVRVLNRDD
ARVMTLASSEQSGAQLVTFGVNEPVRDGDYGLLRDNGMIWLVQAQDRDATDEPAPTRRRKSESTAPPDIGLKRLMPADAL
RIRGLHNATNALAAFALARAIGLPGAPLLHGLREYRGEPHRVELIASIEGVDYVDDSKGTNVGATVAALDGLAQRVVLIA
GGDGKGQAFDPLAEPVTRWCRCVMLIGRDAPQIRAALEHTGIAITDHATLEEATRAASAVAQPGDAVLLSPACASFDMFK
GYAHRAAVFRSTVEDIAAGRGTMI

Sequences:

>Translated_504_residues
MFGEKFRDRQKPMVLVLGLGESGLAMARWCARHGCRLRIADTREVPPNLSALEAHGIEGNFVGGPFSEVLLEGVELVAIS
PGLSPLAADLVPLIATARERGIPVWGELEFFAQALKTLGESGYAPKVIAITGTNGKTTTTSLTGLLCERAGKKVAVAGNI
SPAALDKLTEAIDNTALPDVWVLELSSFQLETAHTFEPDAAVILNITQDHLDWHGGLDAYAAAKGKIFGKNTVRVLNRDD
ARVMTLASSEQSGAQLVTFGVNEPVRDGDYGLLRDNGMIWLVQAQDRDATDEPAPTRRRKSESTAPPDIGLKRLMPADAL
RIRGLHNATNALAAFALARAIGLPGAPLLHGLREYRGEPHRVELIASIEGVDYVDDSKGTNVGATVAALDGLAQRVVLIA
GGDGKGQAFDPLAEPVTRWCRCVMLIGRDAPQIRAALEHTGIAITDHATLEEATRAASAVAQPGDAVLLSPACASFDMFK
GYAHRAAVFRSTVEDIAAGRGTMI
>Mature_504_residues
MFGEKFRDRQKPMVLVLGLGESGLAMARWCARHGCRLRIADTREVPPNLSALEAHGIEGNFVGGPFSEVLLEGVELVAIS
PGLSPLAADLVPLIATARERGIPVWGELEFFAQALKTLGESGYAPKVIAITGTNGKTTTTSLTGLLCERAGKKVAVAGNI
SPAALDKLTEAIDNTALPDVWVLELSSFQLETAHTFEPDAAVILNITQDHLDWHGGLDAYAAAKGKIFGKNTVRVLNRDD
ARVMTLASSEQSGAQLVTFGVNEPVRDGDYGLLRDNGMIWLVQAQDRDATDEPAPTRRRKSESTAPPDIGLKRLMPADAL
RIRGLHNATNALAAFALARAIGLPGAPLLHGLREYRGEPHRVELIASIEGVDYVDDSKGTNVGATVAALDGLAQRVVLIA
GGDGKGQAFDPLAEPVTRWCRCVMLIGRDAPQIRAALEHTGIAITDHATLEEATRAASAVAQPGDAVLLSPACASFDMFK
GYAHRAAVFRSTVEDIAAGRGTMI

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=489, Percent_Identity=37.8323108384458, Blast_Score=234, Evalue=8e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_BURP8 (B2JHG2)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001858893.1
- ProteinModelPortal:   B2JHG2
- SMR:   B2JHG2
- GeneID:   6244158
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2675
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK02006
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 53534; Mature: 53534

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGEKFRDRQKPMVLVLGLGESGLAMARWCARHGCRLRIADTREVPPNLSALEAHGIEGN
CCCCHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHEECCCCCC
FVGGPFSEVLLEGVELVAISPGLSPLAADLVPLIATARERGIPVWGELEFFAQALKTLGE
CCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
SGYAPKVIAITGTNGKTTTTSLTGLLCERAGKKVAVAGNISPAALDKLTEAIDNTALPDV
CCCCCEEEEEECCCCCEEHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCCE
WVLELSSFQLETAHTFEPDAAVILNITQDHLDWHGGLDAYAAAKGKIFGKNTVRVLNRDD
EEEEECCEEEEECCCCCCCCEEEEEEEHHHCCCCCCCCHHHHCCCCEECCCEEEEEECCC
ARVMTLASSEQSGAQLVTFGVNEPVRDGDYGLLRDNGMIWLVQAQDRDATDEPAPTRRRK
CEEEEEECCCCCCCEEEEECCCCCCCCCCCEEEECCCEEEEEEECCCCCCCCCCCCHHCC
SESTAPPDIGLKRLMPADALRIRGLHNATNALAAFALARAIGLPGAPLLHGLREYRGEPH
CCCCCCCCCCHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
RVELIASIEGVDYVDDSKGTNVGATVAALDGLAQRVVLIAGGDGKGQAFDPLAEPVTRWC
EEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCEEEEEEECCCCCCCCCCHHHHHHHHHH
RCVMLIGRDAPQIRAALEHTGIAITDHATLEEATRAASAVAQPGDAVLLSPACASFDMFK
HHHHHHCCCCHHHHHHHHHCCEEEECCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHH
GYAHRAAVFRSTVEDIAAGRGTMI
HHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFGEKFRDRQKPMVLVLGLGESGLAMARWCARHGCRLRIADTREVPPNLSALEAHGIEGN
CCCCHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHEECCCCCC
FVGGPFSEVLLEGVELVAISPGLSPLAADLVPLIATARERGIPVWGELEFFAQALKTLGE
CCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
SGYAPKVIAITGTNGKTTTTSLTGLLCERAGKKVAVAGNISPAALDKLTEAIDNTALPDV
CCCCCEEEEEECCCCCEEHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCCE
WVLELSSFQLETAHTFEPDAAVILNITQDHLDWHGGLDAYAAAKGKIFGKNTVRVLNRDD
EEEEECCEEEEECCCCCCCCEEEEEEEHHHCCCCCCCCHHHHCCCCEECCCEEEEEECCC
ARVMTLASSEQSGAQLVTFGVNEPVRDGDYGLLRDNGMIWLVQAQDRDATDEPAPTRRRK
CEEEEEECCCCCCCEEEEECCCCCCCCCCCEEEECCCEEEEEEECCCCCCCCCCCCHHCC
SESTAPPDIGLKRLMPADALRIRGLHNATNALAAFALARAIGLPGAPLLHGLREYRGEPH
CCCCCCCCCCHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
RVELIASIEGVDYVDDSKGTNVGATVAALDGLAQRVVLIAGGDGKGQAFDPLAEPVTRWC
EEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCEEEEEEECCCCCCCCCCHHHHHHHHHH
RCVMLIGRDAPQIRAALEHTGIAITDHATLEEATRAASAVAQPGDAVLLSPACASFDMFK
HHHHHHCCCCHHHHHHHHHCCEEEECCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHH
GYAHRAAVFRSTVEDIAAGRGTMI
HHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA