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The map label for this gene is gltK [H]

Identifier: 186477319

GI number: 186477319

Start: 2891166

End: 2891843

Strand: Reverse

Name: gltK [H]

Synonym: Bphy_2571

Alternate gene names: 186477319

Gene position: 2891843-2891166 (Counterclockwise)

Preceding gene: 186477320

Following gene: 186477318

Centisome position: 83.12

GC content: 60.47

Gene sequence:

>678_bases
ATGCATCATTTCGACTGGAGCGGTATTCCGGGCGCACTGCCGACGCTGTGGACGGGTGCCATCGTCACGCTCAAGATCAC
GATTCTCGCGATTGTGATCGGCATCGTGTGGGGGACCGTGCTCGCGCTGTTGCGCCTTTCGGGCATCAAGCCGCTGGAGT
GGTTTGCGAAACTTTACGTGACGCTATTCCGCTCGATCCCGCTGGTGATGGTGCTGCTGTGGTTCTTCCTGATCGTGCCG
CAGGTGCTGCAGAACGTGCTGGGCCTTTCCGCGGACATCGACATTCGCCTCGCCTCGGCGATGGTCGCGTTCTCGCTGTT
CGAAGCCGCGTACTATTCGGAAATCATCCGTGCGGGTATTCAGGCCGTGCCGCGCGGGCAAGTAAACGCGTCGTACGCGC
TCGGCATGAGCTATCCGCAGGCGATGCGTCTCGTCGTGCTGCCGCAGGCGTTCCGCGCGATGGTGCCGCTGCTGCTCACG
CAGGCCATCGTGCTCTTTCAGGATACGTCGCTCGTCTACGTGATCAGCCTCGCCGACTTCTTCCGCACGGCCACGAATAT
TGGCGACCGTGACGGTACGAACGTCGAGATGGTACTGTTCGCCGGTGCGTGTTACTTCGTGATCTGCGTGGTCGCGTCGA
GCCTCGTCAAAAGTCTTCAGAAAAAGGTCGCAAGATGA

Upstream 100 bases:

>100_bases
CCGTCACGGTTGCGTATCTGCTCATCAACCTGATCGTGATGACGCTGATGCGCATCGTCGAAGCCAAGAGCCGGCTGCCT
GGCTATATCGGAGGCAAGTG

Downstream 100 bases:

>100_bases
TCTCTATCAAGAATGTGTCCAAGTGGTACGGTCAGTTCCAGGTGCTGACCGACTGCACGACGGAAATCAAAAAGGGCGAA
GTGGTCGTCGTGTGCGGCCC

Product: polar amino acid ABC transporter inner membrane subunit

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MHHFDWSGIPGALPTLWTGAIVTLKITILAIVIGIVWGTVLALLRLSGIKPLEWFAKLYVTLFRSIPLVMVLLWFFLIVP
QVLQNVLGLSADIDIRLASAMVAFSLFEAAYYSEIIRAGIQAVPRGQVNASYALGMSYPQAMRLVVLPQAFRAMVPLLLT
QAIVLFQDTSLVYVISLADFFRTATNIGDRDGTNVEMVLFAGACYFVICVVASSLVKSLQKKVAR

Sequences:

>Translated_225_residues
MHHFDWSGIPGALPTLWTGAIVTLKITILAIVIGIVWGTVLALLRLSGIKPLEWFAKLYVTLFRSIPLVMVLLWFFLIVP
QVLQNVLGLSADIDIRLASAMVAFSLFEAAYYSEIIRAGIQAVPRGQVNASYALGMSYPQAMRLVVLPQAFRAMVPLLLT
QAIVLFQDTSLVYVISLADFFRTATNIGDRDGTNVEMVLFAGACYFVICVVASSLVKSLQKKVAR
>Mature_225_residues
MHHFDWSGIPGALPTLWTGAIVTLKITILAIVIGIVWGTVLALLRLSGIKPLEWFAKLYVTLFRSIPLVMVLLWFFLIVP
QVLQNVLGLSADIDIRLASAMVAFSLFEAAYYSEIIRAGIQAVPRGQVNASYALGMSYPQAMRLVVLPQAFRAMVPLLLT
QAIVLFQDTSLVYVISLADFFRTATNIGDRDGTNVEMVLFAGACYFVICVVASSLVKSLQKKVAR

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786873, Length=224, Percent_Identity=69.6428571428571, Blast_Score=316, Evalue=6e-88,
Organism=Escherichia coli, GI1788226, Length=214, Percent_Identity=35.981308411215, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI87082239, Length=209, Percent_Identity=34.4497607655502, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786874, Length=179, Percent_Identity=34.0782122905028, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1787030, Length=221, Percent_Identity=29.4117647058824, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788645, Length=217, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787087, Length=227, Percent_Identity=27.3127753303965, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI48994930, Length=130, Percent_Identity=33.0769230769231, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788646, Length=208, Percent_Identity=25.9615384615385, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1787086, Length=212, Percent_Identity=25, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 24739; Mature: 24739

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHFDWSGIPGALPTLWTGAIVTLKITILAIVIGIVWGTVLALLRLSGIKPLEWFAKLYV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
TLFRSIPLVMVLLWFFLIVPQVLQNVLGLSADIDIRLASAMVAFSLFEAAYYSEIIRAGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAVPRGQVNASYALGMSYPQAMRLVVLPQAFRAMVPLLLTQAIVLFQDTSLVYVISLADF
HHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FRTATNIGDRDGTNVEMVLFAGACYFVICVVASSLVKSLQKKVAR
HHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHHFDWSGIPGALPTLWTGAIVTLKITILAIVIGIVWGTVLALLRLSGIKPLEWFAKLYV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
TLFRSIPLVMVLLWFFLIVPQVLQNVLGLSADIDIRLASAMVAFSLFEAAYYSEIIRAGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAVPRGQVNASYALGMSYPQAMRLVVLPQAFRAMVPLLLTQAIVLFQDTSLVYVISLADF
HHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FRTATNIGDRDGTNVEMVLFAGACYFVICVVASSLVKSLQKKVAR
HHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]