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The map label for this gene is ruvC

Identifier: 186477302

GI number: 186477302

Start: 2876555

End: 2877097

Strand: Direct

Name: ruvC

Synonym: Bphy_2554

Alternate gene names: 186477302

Gene position: 2876555-2877097 (Clockwise)

Preceding gene: 186477301

Following gene: 186477304

Centisome position: 82.68

GC content: 63.35

Gene sequence:

>543_bases
ATGAGAATTCTCGGCATTGACCCTGGCTTGCGCGTGACCGGCTTCGGCGTGATCGATCAGTCCGGCCATCAGCTGACTTA
CGTGACGAGCGGCGTCATCAAAACAGCCGACGCCGATCTGCCGACGCGCCTGGGCACGATCTTCGAGGGTATTTCGACGC
TGATTCGCCAACATGCGCCCGACCACTCCGCGATCGAGAAGGTGTTCGTCAACGTCAATCCGCAGTCGACGCTGCTGCTC
GGCCAGGCGCGCGGCGCGGCGATTTGCGGACTGGTGTCGGGCGGCGTGCCCGTTGCCGAATACACGGCGCTGCAACTGAA
ACAGGCCGTGGTCGGCTACGGACGCGCGACCAAGGAGCAGATGCAACAGATGGTCGTGCGGCTGTTATGCCTGTCGGGCA
TTCCGTCCACGGATGCCGCCGACGCACTCGGCATGGCGATTTGCCATGCGCATGGCGGCGGCACATTGAGCACACTGGGT
GGTATTGCACCGGGACTCGCGAAAAAGGGCTTGCGAGTGCGTCGCGGGCGGTTGGTAGGTTAG

Upstream 100 bases:

>100_bases
GTTCTGCTTGTTCCGCAACGGCCCGGCAGTTTGCAACTGCCGGGCCGTTTTTGCATTTGATTCGTTGTAGTATCGCAGGC
ACTGAATACGCACGGCACTT

Downstream 100 bases:

>100_bases
GCGCCTTTGCCGTCAGCGTTGTCCCGCCAGTGCCAGCCGACACGCCAGGCCGGCGAACACCGAGCCGAGCAAATATTGCG
GCCAGCGCGGCGGGCGTTCG

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MRILGIDPGLRVTGFGVIDQSGHQLTYVTSGVIKTADADLPTRLGTIFEGISTLIRQHAPDHSAIEKVFVNVNPQSTLLL
GQARGAAICGLVSGGVPVAEYTALQLKQAVVGYGRATKEQMQQMVVRLLCLSGIPSTDAADALGMAICHAHGGGTLSTLG
GIAPGLAKKGLRVRRGRLVG

Sequences:

>Translated_180_residues
MRILGIDPGLRVTGFGVIDQSGHQLTYVTSGVIKTADADLPTRLGTIFEGISTLIRQHAPDHSAIEKVFVNVNPQSTLLL
GQARGAAICGLVSGGVPVAEYTALQLKQAVVGYGRATKEQMQQMVVRLLCLSGIPSTDAADALGMAICHAHGGGTLSTLG
GIAPGLAKKGLRVRRGRLVG
>Mature_180_residues
MRILGIDPGLRVTGFGVIDQSGHQLTYVTSGVIKTADADLPTRLGTIFEGISTLIRQHAPDHSAIEKVFVNVNPQSTLLL
GQARGAAICGLVSGGVPVAEYTALQLKQAVVGYGRATKEQMQQMVVRLLCLSGIPSTDAADALGMAICHAHGGGTLSTLG
GIAPGLAKKGLRVRRGRLVG

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=176, Percent_Identity=50.5681818181818, Blast_Score=140, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_BURP8 (B2JGU2)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001858772.1
- ProteinModelPortal:   B2JGU2
- SMR:   B2JGU2
- GeneID:   6244037
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2554
- HOGENOM:   HBG437552
- OMA:   DCAAIEK
- ProtClustDB:   PRK00039
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18649; Mature: 18649

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILGIDPGLRVTGFGVIDQSGHQLTYVTSGVIKTADADLPTRLGTIFEGISTLIRQHAP
CEEECCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
DHSAIEKVFVNVNPQSTLLLGQARGAAICGLVSGGVPVAEYTALQLKQAVVGYGRATKEQ
CHHHHHHEEEECCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHH
MQQMVVRLLCLSGIPSTDAADALGMAICHAHGGGTLSTLGGIAPGLAKKGLRVRRGRLVG
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHCCCHHHCCCCCC
>Mature Secondary Structure
MRILGIDPGLRVTGFGVIDQSGHQLTYVTSGVIKTADADLPTRLGTIFEGISTLIRQHAP
CEEECCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
DHSAIEKVFVNVNPQSTLLLGQARGAAICGLVSGGVPVAEYTALQLKQAVVGYGRATKEQ
CHHHHHHEEEECCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHH
MQQMVVRLLCLSGIPSTDAADALGMAICHAHGGGTLSTLGGIAPGLAKKGLRVRRGRLVG
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA