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The map label for this gene is purH [H]

Identifier: 186477301

GI number: 186477301

Start: 2874883

End: 2876448

Strand: Direct

Name: purH [H]

Synonym: Bphy_2553

Alternate gene names: 186477301

Gene position: 2874883-2876448 (Clockwise)

Preceding gene: 186477300

Following gene: 186477302

Centisome position: 82.63

GC content: 63.28

Gene sequence:

>1566_bases
ATGATCAAGCAAGCGCTCATCTCCGTTTCCGACAAGTCCGGCATCGTCGATTTCGCGAAATCGCTGTCGGACCTCGGCGT
CAAGATCCTGTCGACGGGCGGCACCGCGAAACTGCTCGCGGACGCCGGCCTTCCTGTTACCGAAGTGGCCGACTACACGG
GCTTTCCGGAAATGCTGGATGGGCGCGTGAAGACGCTGCACCCGAAGGTGCACGGCGGCATCCTGGCTCGCCGCGATCTG
CCCGAGCACATGGCCGCGCTCGAAAAGCACGACATTCCGACCATCGACCTGCTCGTCGTGAACCTGTACCCGTTCGTGCA
GACTGTGTCGAAGGAAGAATGCACGCTCGAAGACGCGATCGAGAACATCGATATCGGCGGCCCGACCATGCTGCGTTCGG
CGGCGAAGAATCATCGTGACGTGACCGTCGTCGTCGACCCGGCCGACTATGCGACCGTGCTCGACGAAATGCGCGCAAAC
GGCAACACGGTCGGCTACAAGACGAACTTCCGCCTCGCGACCAAGGTGTTCGCGCACACCGCGCAGTACGATGGCGCGAT
TACGAACTACCTGACGAGCCTCACGGAACAGCTGCAACACCGTGACCGCAACACGTACCCGGCCACGCTGAACATGGCGT
TCGAGAAGGTGCAGGATCTGCGCTACGGTGAGAATCCGCACCAAAGCGCTGCGTTCTATCGCGATATCGCGGCGCCTGCT
GGCGCGCTGGCCAACTATCGCCAGCTGCAGGGCAAGGAACTGTCGTACAACAACATCGCCGACTCGGATGCCGCATGGGA
ATGCGTGAAGACGTTCGACGCGCCCGCCTGCGTGATCATCAAGCACGCGAACCCGTGCGGCGTGGCCGTCGGCGCGAACC
CGCACGAAGCGTATTCGAAGGCGTTCCAGACCGATCCGACCTCGGCGTTCGGCGGCATCATCGCGTTCAACCGCGAAGTC
GACGAAACGGCCGCGCAGGCTGTCGCGAAGCAGTTCGTCGAAGTGCTGATCGCGCCGTCGTTCAGCGAAGCGGCGAAGCA
ACTGTTCGCCGCCAAGCAGAACGTGCGTCTGCTCGAAATCGCGCTGGGTGAAGGCCATAACGCATTCGATCTGAAGCGCG
TGGGCGGCGGTCTGCTGGTGCAATCGCTCGATGCGAAGAACGTGCAGCCGCACGAACTGCGCGTCGTCACGAAACGTCAT
CCGACGCCGAAGGAAATGGACGACTTGCTGTTCGCATGGCGGGTGGCGAAGTTCGTGAAGTCGAACGCGATCGTGTTCTG
CGCGAACGGCATGACGATGGGCGTCGGCGCCGGACAGATGAGCCGCGTGGACTCGGCGCGCATCGCGAGCATCAAGGCGC
AGAACGCCGGCCTGACGCTCTCGGGTACGGCGGTGGCGTCGGATGCGTTCTTCCCGTTCCGCGACGGCCTCGACGTGGTC
GTGAACGCGGGCGCAACCTGCGTCATCCAGCCGGGCGGCTCGATGCGCGACGACGAAGTGATCGCCGCCGCCGACGAGCA
CAATATCGCAATGGTCGTGACGGGCATTCGCCACTTCCGCCACTGA

Upstream 100 bases:

>100_bases
TCGGCATCAACCGCAATACGCTGCGCAAGAAGCTGCAACAGCACGGCCTGCTGTAGTTTCGGCGCCCTGCCACGCCTTTC
ATCGGCTATTTGTTCCCATC

Downstream 100 bases:

>100_bases
TCGGCTGTTCTGCTTGTTCCGCAACGGCCCGGCAGTTTGCAACTGCCGGGCCGTTTTTGCATTTGATTCGTTGTAGTATC
GCAGGCACTGAATACGCACG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MIKQALISVSDKSGIVDFAKSLSDLGVKILSTGGTAKLLADAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMAALEKHDIPTIDLLVVNLYPFVQTVSKEECTLEDAIENIDIGGPTMLRSAAKNHRDVTVVVDPADYATVLDEMRAN
GNTVGYKTNFRLATKVFAHTAQYDGAITNYLTSLTEQLQHRDRNTYPATLNMAFEKVQDLRYGENPHQSAAFYRDIAAPA
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGANPHEAYSKAFQTDPTSAFGGIIAFNREV
DETAAQAVAKQFVEVLIAPSFSEAAKQLFAAKQNVRLLEIALGEGHNAFDLKRVGGGLLVQSLDAKNVQPHELRVVTKRH
PTPKEMDDLLFAWRVAKFVKSNAIVFCANGMTMGVGAGQMSRVDSARIASIKAQNAGLTLSGTAVASDAFFPFRDGLDVV
VNAGATCVIQPGGSMRDDEVIAAADEHNIAMVVTGIRHFRH

Sequences:

>Translated_521_residues
MIKQALISVSDKSGIVDFAKSLSDLGVKILSTGGTAKLLADAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMAALEKHDIPTIDLLVVNLYPFVQTVSKEECTLEDAIENIDIGGPTMLRSAAKNHRDVTVVVDPADYATVLDEMRAN
GNTVGYKTNFRLATKVFAHTAQYDGAITNYLTSLTEQLQHRDRNTYPATLNMAFEKVQDLRYGENPHQSAAFYRDIAAPA
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGANPHEAYSKAFQTDPTSAFGGIIAFNREV
DETAAQAVAKQFVEVLIAPSFSEAAKQLFAAKQNVRLLEIALGEGHNAFDLKRVGGGLLVQSLDAKNVQPHELRVVTKRH
PTPKEMDDLLFAWRVAKFVKSNAIVFCANGMTMGVGAGQMSRVDSARIASIKAQNAGLTLSGTAVASDAFFPFRDGLDVV
VNAGATCVIQPGGSMRDDEVIAAADEHNIAMVVTGIRHFRH
>Mature_521_residues
MIKQALISVSDKSGIVDFAKSLSDLGVKILSTGGTAKLLADAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMAALEKHDIPTIDLLVVNLYPFVQTVSKEECTLEDAIENIDIGGPTMLRSAAKNHRDVTVVVDPADYATVLDEMRAN
GNTVGYKTNFRLATKVFAHTAQYDGAITNYLTSLTEQLQHRDRNTYPATLNMAFEKVQDLRYGENPHQSAAFYRDIAAPA
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGANPHEAYSKAFQTDPTSAFGGIIAFNREV
DETAAQAVAKQFVEVLIAPSFSEAAKQLFAAKQNVRLLEIALGEGHNAFDLKRVGGGLLVQSLDAKNVQPHELRVVTKRH
PTPKEMDDLLFAWRVAKFVKSNAIVFCANGMTMGVGAGQMSRVDSARIASIKAQNAGLTLSGTAVASDAFFPFRDGLDVV
VNAGATCVIQPGGSMRDDEVIAAADEHNIAMVVTGIRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=478, Percent_Identity=37.0292887029289, Blast_Score=274, Evalue=1e-73,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=61.7142857142857, Blast_Score=655, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=518, Percent_Identity=34.7490347490347, Blast_Score=262, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI71985574, Length=350, Percent_Identity=26.2857142857143, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71985556, Length=130, Percent_Identity=33.8461538461538, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=480, Percent_Identity=35.4166666666667, Blast_Score=260, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6323056, Length=489, Percent_Identity=35.7873210633947, Blast_Score=255, Evalue=9e-69,
Organism=Drosophila melanogaster, GI24649832, Length=485, Percent_Identity=37.1134020618557, Blast_Score=270, Evalue=2e-72,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56217; Mature: 56217

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKQALISVSDKSGIVDFAKSLSDLGVKILSTGGTAKLLADAGLPVTEVADYTGFPEMLD
CCCHHEEECCCCCCHHHHHHHHHHCCEEEEECCCCEEEHCCCCCCHHHHHHCCCCHHHHC
GRVKTLHPKVHGGILARRDLPEHMAALEKHDIPTIDLLVVNLYPFVQTVSKEECTLEDAI
CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHH
ENIDIGGPTMLRSAAKNHRDVTVVVDPADYATVLDEMRANGNTVGYKTNFRLATKVFAHT
HHCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHH
AQYDGAITNYLTSLTEQLQHRDRNTYPATLNMAFEKVQDLRYGENPHQSAAFYRDIAAPA
HHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCH
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGANPHEAYSK
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEECCCHHHHHHH
AFQTDPTSAFGGIIAFNREVDETAAQAVAKQFVEVLIAPSFSEAAKQLFAAKQNVRLLEI
HHCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEE
ALGEGHNAFDLKRVGGGLLVQSLDAKNVQPHELRVVTKRHPTPKEMDDLLFAWRVAKFVK
EECCCCCCEEHHHCCCCCEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
SNAIVFCANGMTMGVGAGQMSRVDSARIASIKAQNAGLTLSGTAVASDAFFPFRDGLDVV
CCCEEEEECCCEEECCCCHHHHCCCHHHEEEEECCCCEEEECCEEECCCCCCCCCCCCEE
VNAGATCVIQPGGSMRDDEVIAAADEHNIAMVVTGIRHFRH
EECCCEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC
>Mature Secondary Structure
MIKQALISVSDKSGIVDFAKSLSDLGVKILSTGGTAKLLADAGLPVTEVADYTGFPEMLD
CCCHHEEECCCCCCHHHHHHHHHHCCEEEEECCCCEEEHCCCCCCHHHHHHCCCCHHHHC
GRVKTLHPKVHGGILARRDLPEHMAALEKHDIPTIDLLVVNLYPFVQTVSKEECTLEDAI
CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHH
ENIDIGGPTMLRSAAKNHRDVTVVVDPADYATVLDEMRANGNTVGYKTNFRLATKVFAHT
HHCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHH
AQYDGAITNYLTSLTEQLQHRDRNTYPATLNMAFEKVQDLRYGENPHQSAAFYRDIAAPA
HHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCH
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGANPHEAYSK
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEECCCHHHHHHH
AFQTDPTSAFGGIIAFNREVDETAAQAVAKQFVEVLIAPSFSEAAKQLFAAKQNVRLLEI
HHCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEE
ALGEGHNAFDLKRVGGGLLVQSLDAKNVQPHELRVVTKRHPTPKEMDDLLFAWRVAKFVK
EECCCCCCEEHHHCCCCCEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
SNAIVFCANGMTMGVGAGQMSRVDSARIASIKAQNAGLTLSGTAVASDAFFPFRDGLDVV
CCCEEEEECCCEEECCCCHHHHCCCHHHEEEEECCCCEEEECCEEECCCCCCCCCCCCEE
VNAGATCVIQPGGSMRDDEVIAAADEHNIAMVVTGIRHFRH
EECCCEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA