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The map label for this gene is ppnK

Identifier: 186477253

GI number: 186477253

Start: 2815259

End: 2816161

Strand: Direct

Name: ppnK

Synonym: Bphy_2504

Alternate gene names: 186477253

Gene position: 2815259-2816161 (Clockwise)

Preceding gene: 186477249

Following gene: 186477254

Centisome position: 80.92

GC content: 64.67

Gene sequence:

>903_bases
ATGCAAGCTAACAGCCAGTTCAAGACCGTTGCGCTCGTCGGGCGCAGCAACACGCCGGGCATCGGGGAACCGCTGACCGC
GCTCGCCACGTGCATCGAGAAGCTGGGCTTCGAGATCGCGTTCGAGGCGGAAACCGCGCAGGAAATCGGCGTCTCGCGCT
GGCCCGCGCTGCAGCCGGCGGAAATTGGCGCGCGCGCGGACGTGGCGATCGTTCTCGGCGGCGACGGCACGATGCTCGGC
ATCGGCCGACAGCTGGCGCCATACAAGACACCGCTGATCGGCATCAACCACGGCCGGCTCGGCTTCATCACCGACATCCC
GTTCTCCGACATGCGCGAGATCATCCCGCAGATGCTGTCGGGCAGCTTCGAACGGGAAGAGCGCTCGCTGCTCGAATCGC
GGATCATGCGCGACGGCCAGCCGATCTACCACGCGCTCGCGTTCAACGACGTCGTTGTGAACCGTTCGGGCTTTTCGGGC
ATGGCCGAACTGCGCGTGTCGGTGGACGGACGCTTCATGTACAACCAGCGCTCGGACGGGTTGATCGTCGCCACGCCGAC
GGGCTCGACGGCCTACGCGCTGTCGTCGCAAGGGCCGATTCTGCATCCGCAGCTGCAGGGCCTCGTGCTCGTGCCGATTG
CCCCGCACGCACTGTCCAACCGGCCTATCGTGATTCCGGACGACTCGAAGGTCAGCATCCAGATCATCTCTGGCCGCGAC
GTCAACGTGAACTTCGACATGCAGTCGTTCACCGCGCTGGAGCTGAACGACACGATCGACGTGCGCCGCTCACGCCATAC
CGTGCCTTTCCTGCATCCCGTCGGCTACAGCTATTACGCGACGCTGCGCAAGAAGCTGCACTGGAACGAGTACCCGTCAC
ACGAAGACGACCCGGACGACTGA

Upstream 100 bases:

>100_bases
AGGCTGGCCGCCAACACGCGGCCGCCGGGCATCCGCCCCAGCCGGCGCGCCGCCGCCGCCAACGGTTCTTTTCAGGTCGC
ACCTATGGTGTAATGCCGGC

Downstream 100 bases:

>100_bases
ACGCACACCGGACACAAGCGGCGCAGCCAGTCCGCAGAGCCCGAACGTCACCGCAAACGACCAGACCAACTCACGCAAGC
CACGCCAGACACACGAGCAT

Product: NAD(+)/NADH kinase family protein

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MQANSQFKTVALVGRSNTPGIGEPLTALATCIEKLGFEIAFEAETAQEIGVSRWPALQPAEIGARADVAIVLGGDGTMLG
IGRQLAPYKTPLIGINHGRLGFITDIPFSDMREIIPQMLSGSFEREERSLLESRIMRDGQPIYHALAFNDVVVNRSGFSG
MAELRVSVDGRFMYNQRSDGLIVATPTGSTAYALSSQGPILHPQLQGLVLVPIAPHALSNRPIVIPDDSKVSIQIISGRD
VNVNFDMQSFTALELNDTIDVRRSRHTVPFLHPVGYSYYATLRKKLHWNEYPSHEDDPDD

Sequences:

>Translated_300_residues
MQANSQFKTVALVGRSNTPGIGEPLTALATCIEKLGFEIAFEAETAQEIGVSRWPALQPAEIGARADVAIVLGGDGTMLG
IGRQLAPYKTPLIGINHGRLGFITDIPFSDMREIIPQMLSGSFEREERSLLESRIMRDGQPIYHALAFNDVVVNRSGFSG
MAELRVSVDGRFMYNQRSDGLIVATPTGSTAYALSSQGPILHPQLQGLVLVPIAPHALSNRPIVIPDDSKVSIQIISGRD
VNVNFDMQSFTALELNDTIDVRRSRHTVPFLHPVGYSYYATLRKKLHWNEYPSHEDDPDD
>Mature_300_residues
MQANSQFKTVALVGRSNTPGIGEPLTALATCIEKLGFEIAFEAETAQEIGVSRWPALQPAEIGARADVAIVLGGDGTMLG
IGRQLAPYKTPLIGINHGRLGFITDIPFSDMREIIPQMLSGSFEREERSLLESRIMRDGQPIYHALAFNDVVVNRSGFSG
MAELRVSVDGRFMYNQRSDGLIVATPTGSTAYALSSQGPILHPQLQGLVLVPIAPHALSNRPIVIPDDSKVSIQIISGRD
VNVNFDMQSFTALELNDTIDVRRSRHTVPFLHPVGYSYYATLRKKLHWNEYPSHEDDPDD

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=260, Percent_Identity=28.4615384615385, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1788968, Length=288, Percent_Identity=37.5, Blast_Score=201, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6320794, Length=241, Percent_Identity=29.4605809128631, Blast_Score=125, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6322509, Length=267, Percent_Identity=29.5880149812734, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6325068, Length=177, Percent_Identity=32.2033898305085, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28573828, Length=251, Percent_Identity=30.2788844621514, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28573832, Length=251, Percent_Identity=30.2788844621514, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI161077047, Length=251, Percent_Identity=30.2788844621514, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI28573830, Length=251, Percent_Identity=30.2788844621514, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI28573826, Length=251, Percent_Identity=30.2788844621514, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI20129957, Length=276, Percent_Identity=27.8985507246377, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281363321, Length=276, Percent_Identity=27.8985507246377, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281363323, Length=276, Percent_Identity=27.8985507246377, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653424, Length=276, Percent_Identity=27.8985507246377, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653422, Length=276, Percent_Identity=27.8985507246377, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_BURP8 (B2JGE9)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001858723.1
- ProteinModelPortal:   B2JGE9
- SMR:   B2JGE9
- GeneID:   6243988
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2504
- HOGENOM:   HBG713904
- OMA:   FESFMID
- ProtClustDB:   PRK02155
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 32940; Mature: 32940

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQANSQFKTVALVGRSNTPGIGEPLTALATCIEKLGFEIAFEAETAQEIGVSRWPALQPA
CCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHCCCCCCCCCCCH
EIGARADVAIVLGGDGTMLGIGRQLAPYKTPLIGINHGRLGFITDIPFSDMREIIPQMLS
HCCCCCCEEEEECCCCEEEECCCCCCCCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHC
GSFEREERSLLESRIMRDGQPIYHALAFNDVVVNRSGFSGMAELRVSVDGRFMYNQRSDG
CCCCHHHHHHHHHHHHHCCCCEEEEEEECEEEEECCCCCCEEEEEEEECCEEEECCCCCC
LIVATPTGSTAYALSSQGPILHPQLQGLVLVPIAPHALSNRPIVIPDDSKVSIQIISGRD
EEEECCCCCEEEEECCCCCEECCCCCCEEEEEECCHHCCCCCEEEECCCEEEEEEECCCE
VNVNFDMQSFTALELNDTIDVRRSRHTVPFLHPVGYSYYATLRKKLHWNEYPSHEDDPDD
EEEEECCCCEEEEEECCCEEHHHCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQANSQFKTVALVGRSNTPGIGEPLTALATCIEKLGFEIAFEAETAQEIGVSRWPALQPA
CCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHCCCCCCCCCCCH
EIGARADVAIVLGGDGTMLGIGRQLAPYKTPLIGINHGRLGFITDIPFSDMREIIPQMLS
HCCCCCCEEEEECCCCEEEECCCCCCCCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHC
GSFEREERSLLESRIMRDGQPIYHALAFNDVVVNRSGFSGMAELRVSVDGRFMYNQRSDG
CCCCHHHHHHHHHHHHHCCCCEEEEEEECEEEEECCCCCCEEEEEEEECCEEEECCCCCC
LIVATPTGSTAYALSSQGPILHPQLQGLVLVPIAPHALSNRPIVIPDDSKVSIQIISGRD
EEEECCCCCEEEEECCCCCEECCCCCCEEEEEECCHHCCCCCEEEECCCEEEEEEECCCE
VNVNFDMQSFTALELNDTIDVRRSRHTVPFLHPVGYSYYATLRKKLHWNEYPSHEDDPDD
EEEEECCCCEEEEEECCCEEHHHCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA